Template-Type: ReDIF-Article 1.0 Author-Name: U. Czarnik Author-Workplace-Name: Department of Animal Genetics, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: M. Galiński Author-Workplace-Name: Department of Animal Genetics, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: Ch.S. Pareek Author-Workplace-Name: Department of Animal Genetics, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: T. Zabolewicz Author-Workplace-Name: Department of Animal Genetics, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Author-Name: Z. Wielgosz-Groth Author-Workplace-Name: Department of Cattle Breeding, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland Title: Study of an association between SNP 775C>T within the bovine ITBG2 gene and milk performance traits in Black and White cows Abstract: The exclusive pre-selective effect of BLAD carriers reproducing in the exposed Holstein-Friesian cattle population prompted to search for the candidate gene variants of high quality of milk performance traits within the bovine ITBG2 gene or loci linked with bovine ITBG2 gene. Theoretical considerations indicated that the &Idquo;silent mutation" C→T localized at the position of 775 bp of the gene encoding the CD18 subunit might be a potential QTL marker of high milk productivity. An association study between the polymorphism of SNP 775C>T, and the diversification of milk performance traits was carried out on the progeny of four bulls with genotypes BL/TL and 775C/T and one bull with genotypes TL/TL and 775C/T. The results documented statistically significant differences in the protein content percentage of milk in two half-sib families of bulls with BL/TL and 775C/T genotype and one half-sib family of bull with TL/TL and 775C/T genotype. It was further concluded that the polymorphism of SNP 775C>T was found to be a more efficient QTL marker than that of D128G, since in all the analysed milk performance traits for half-sib families higher values of the F coefficient were obtained for the SNP 775C>T mutation in comparison with D128G. Keywords: BLAD carriers, point mutation, lethal genes, milk performance traits, QTL marker Journal: Czech Journal of Animal Science Pages: 1-6 Volume: 52 Issue: 1 Year: 2007 DOI: 10.17221/2329-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2329-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200701-0001.txt Handle: RePEc:caa:jnlcjs:v:52:y:2007:i:1:id:2329-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: R. Kasarda Author-Workplace-Name: Slovak University of Agriculture, Nitra, Slovak Republic Author-Name: O. Kadlečík Author-Workplace-Name: Slovak University of Agriculture, Nitra, Slovak Republic Title: An economic impact of inbreeding in the purebred population of Pinzgau cattle in Slovakia on milk production traits Abstract: An increase of inbreeding in a small, endangered population is one of the important criterions in the evaluation of the degree of endangerment of the given breed. The aim was to determine an economic impact of inbreeding in the purebred population of Pinzgau cattle in Slovakia on milk production traits. Pedigree information on 1 611 purebred Pinzgau cows under milk recording was used for the analysis. Average production was 3 898 kg of milk, with 3.93% of fat and 3.31% of proteins. The pedigree of each cow was completed maximally to the 4th generation. Simultaneously, the results from a genetic evaluation of milk production traits such as milk production (M) in kg, fat (F) and protein (P) production in kg and the values of Slovak production index (SPI) of 1 611 cows were collected. Cows were born in 1998-2003. The ratio of inbred cows in the purebred population was 5.7%. Their average Fx = 4.225%. Increased inbreeding has a negative impact on SPI and EBV of milk, fat and protein production. The inbreeding depression was -39.60 SKK in SPI, -8.95 kg in EBV of milk, -0.37 kg in EBV of fat and -0.36 kg in EBV of protein. Keywords: endangered breed, selection index, breeding value, inbreeding Journal: Czech Journal of Animal Science Pages: 7-11 Volume: 52 Issue: 1 Year: 2007 DOI: 10.17221/2330-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2330-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200701-0002.txt Handle: RePEc:caa:jnlcjs:v:52:y:2007:i:1:id:2330-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Z. Eliáš Author-Workplace-Name: Slovak University of Agriculture, Nitra, Slovak Republic Author-Name: S. Hluchý Author-Workplace-Name: Slovak University of Agriculture, Nitra, Slovak Republic Author-Name: J. Mlynek Author-Workplace-Name: Slovak University of Agriculture, Nitra, Slovak Republic Title: Histological structure of the musculus longissimus lumborum et thoracis in pigs with the same ryanodine receptor genotype (CC) in relation to carcass indicators Abstract: The aim of this study was to investigate the histological structure of the longissimus muscle in pigs in relation to carcass value indicators. A total number of 16 pigs of about 101.28 kg average live weight were used. The animals were raised at a Fattening and Carcass Value Experimental Station (FCVES) of Slovak University of Agriculture in Nitra in equal conditions, receiving a standard diet fortified with vitamin-mineral mixture, and they were slaughtered in an experimental abattoir of FCVES. Samples from the musculus longissimus lumborum et thoracisi (MLLT) for histological evaluation were taken within 30 minutes post mortem, immediately frozen in liquid nitrogen and stored at a temperature of -20°C. In the experimental abattoir of FCVES feeding indicators, and indicators of meat quality and carcass value were examined. Samples were processed histochemically and single types of muscle fibres were differentiated according to reactions on SDH on the basis of Vacek's (1974) method. Nikon microscopic system, Pixelink digital camera and LUCIA software for image analyses for the morphometric analysis of MLLT structure were used. The highest abundance of white and the lowest abundance of intermediate muscle fibres was obtained in the analyzed musculus longissimus lumborum et thoracisi of pigs. Red muscle fibre abundance was only slightly higher than intermediate muscle fibre abundance. Concerning the average muscle fibre diameter, the highest values in white and the lowest values in red muscle fibres were found. Positive correlations of white muscle fibre abundance with loin meat weight, thigh meat weight, carcass length, ribcase length, hot right half weight, valuable meatiness parts in kilograms, thigh weight, thigh percent in the half-carcass and MLLT area weight were obtained. In the case of all fat content and weight indicators negative correlations were obtained except loin fat weight. Red muscle fibre content showed positive correlations with shoulder fat weight, neck meat weight, neck fat weight, head weight, thigh fat weight, average backfat thickness and MLLT area. Correlation coefficients between white muscle fibre diameter and shoulder meat weight, thigh meat weight, carcass length, ribcase length showed weak positive correlations. An increase in the white muscle fibre diameter corresponds with an increase in loin meat weight, valuable meatiness parts in kg, valuable meatiness parts in percents, thigh weight, thigh percent in the half-carcass weight and MLLT area. Concerning the red muscle fibre diameter weak positive correlations were obtained in relation to neck meat weight, thigh meat weight, thigh weight and moderate positive correlations to shoulder meat weight, loin meat weight, valuable meatiness parts in kilograms and percents, thigh percent in half-carcass and MLLT area. Keywords: pig, MLLT, muscle fibres, carcass value Journal: Czech Journal of Animal Science Pages: 12-20 Volume: 52 Issue: 1 Year: 2007 DOI: 10.17221/2328-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2328-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200701-0003.txt Handle: RePEc:caa:jnlcjs:v:52:y:2007:i:1:id:2328-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Z.G. Wang Author-Workplace-Name: College of Animal Science, Key Laboratory for Molecular Animal Nutrition, Zhejiang University, Hang Zhou, China Author-Name: Z.R. XU Author-Workplace-Name: College of Animal Science, Key Laboratory for Molecular Animal Nutrition, Zhejiang University, Hang Zhou, China Author-Name: S.D. YU Author-Workplace-Name: College of Animal Science, Key Laboratory for Molecular Animal Nutrition, Zhejiang University, Hang Zhou, China Title: Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat Abstract: The oocytes (experiment 1) were harvested by one of the four collection techniques (slicing, puncture, aspiration I and aspiration II) and the total number and the number of each grade of oocytes were counted, respectively. The good-quality oocytes (good and fair grade) were cultured for maturation. In experiment 2, the oocytes were matured in TCM-199 supplemented with 10 ng/ml of epidermal growth factor (EGF) or 10% FCS, either alone or with 1 IU/ml FSH, or the oocytes were matured in TCM-199 supplemented with 1 IU/ml FSH. After maturation, the oocytes in the two experiments were fertilized, respectively. Slicing (6.3) and puncture (5.8) of the ovaries yielded a higher (P < 0.05) number of oocytes per ovary compared to aspiration I (2.9) and aspiration II (3.1). Oocytes matured in the TCM-199 medium supplemented with EGF or FCS with FSH had a significantly higher proportion of blastocysts than the other treatments (P < 0.05). In conclusion, slicing and puncture are alternative techniques of oocyte recovery in Boer goat. The TCM-199 medium supplemented with EGF or FCS in the presence of FSH is suitable for in vitro maturation of oocytes. Keywords: Boer goat, collection techniques, oocyte maturation, ovary, medium Journal: Czech Journal of Animal Science Pages: 21-25 Volume: 52 Issue: 1 Year: 2007 DOI: 10.17221/2327-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2327-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200701-0004.txt Handle: RePEc:caa:jnlcjs:v:52:y:2007:i:1:id:2327-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: E. Tůmová Author-Workplace-Name: Department of Animal Husbandry, Czech University of Agriculture, Prague, Czech Republic Author-Name: L. Zita Author-Workplace-Name: Department of Animal Husbandry, Czech University of Agriculture, Prague, Czech Republic Author-Name: M. Hubený Author-Workplace-Name: Department of Animal Husbandry, Czech University of Agriculture, Prague, Czech Republic Author-Name: M. Skřivan Author-Workplace-Name: Department of Animal Husbandry, Czech University of Agriculture, Prague, Czech Republic Author-Name: Z. Ledvinka Author-Workplace-Name: Department of Animal Husbandry, Czech University of Agriculture, Prague, Czech Republic Title: The effect of oviposition time and genotype on egg quality characteristics in egg type hens Abstract: In an experiment with three strains of Dominant genotype the effect of oviposition on egg quality characteristics was investigated. Laying hens of the Blue strain (group 1), Plymouth Rock (group 2) and their F1 cross (group 3) from 20 to 64 weeks of age were housed on litter (9 birds per m2). The collection time was at 6.00, 10.00 and 14.00 h. Eggs were gathered in a 28-day interval, every two days all produced eggs (720 eggs in total). The time of oviposition was influenced by genotype. The highest number of eggs was collected in the Plymouth Rock strain at 6.00 (53.5%) and the lowest in the Blue strain at 14.00 (11.1%). There were interactions between the time of oviposition and genotype in egg weight. Eggs laid in the morning at 6.00 were significantly heavier (60.5 g, 64.9 g and 62.1 g) in comparison with eggs laid in the afternoon (59.3 g, 62.4 g and 62.7 g). The heaviest eggs were produced by the Plymouth Rock strain. Significant interactions were observed in eggshell weight when the heaviest eggshell was in afternoon eggs (5.6 g, 5.8 and 5.9 g) and F1 genotype. The egg shape index was also affected by the genotype and time of oviposition. Among the albumen quality characteristics only Haugh Units were influenced by genotype and significantly higher numbers were determined in the Plymouth Rock strain (85.0, 85.3 and 84.7). The genotype significantly affected also the yolk index which was the highest in F1 genotype (45.4%, 45.4% and 44.5%). Keywords: laying hens, genotype, oviposition time, egg quality Journal: Czech Journal of Animal Science Pages: 26-30 Volume: 52 Issue: 1 Year: 2007 DOI: 10.17221/2326-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2326-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200701-0005.txt Handle: RePEc:caa:jnlcjs:v:52:y:2007:i:1:id:2326-CJAS