Template-Type: ReDIF-Article 1.0 Author-Name: B. Mioč Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, University of Zagreb, Croatia Author-Name: V. Pavić Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, University of Zagreb, Croatia Author-Name: I. Vnučec Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, University of Zagreb, Croatia Author-Name: Z. Prpić Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, University of Zagreb, Croatia Author-Name: A. Kostelić Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, University of Zagreb, Croatia Author-Name: V. Sušić Author-Workplace-Name: Department of Animal Husbandry, Faculty of Veterinary Medicine, University of Zagreb, Croatia Title: Effect of olive cake on daily gain, carcass characteristics and chemical composition of lamb meat Abstract: This experiment investigated the effect of olive cake in the feed of weaned Pramenka lambs on their daily gain, carcass traits and on the chemical composition of different groups of muscles. For that purpose, 45 male lambs were divided into three groups with 15 lambs each and fed separately for 50 days. The first group (control) was fed a commercial concentrate, while the second and third (test) groups were fed a concentrate with the addition of 15% and 30% olive cake, respectively. The results showed that the high level of olive cake inclusion (30%) decreased (P < 0.01) daily gain, final weight, empty carcass weight and (P < 0.05) dressing percentage of lambs. The olive cake resulted in a significantly higher (P < 0.01) proportion of stomach and intestines in the carcass compared to the control. The content of fat, proteins and dry matter in all the analyzed groups of muscles was lowest in the carcasses of lambs fed olive cake. The results of this research suggest that the addition of 15% of olive cake to the concentrate had no significant negative effect on daily gain, carcass weight and dressing percentage of lambs. Keywords: olive cake, Pramenka lambs, fattening, carcass traits, meat chemical composition Journal: Czech Journal of Animal Science Pages: 31-36 Volume: 52 Issue: 2 Year: 2007 DOI: 10.17221/2261-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2261-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200702-0001.txt Handle: RePEc:caa:jnlcjs:v:52:y:2007:i:2:id:2261-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: D. Húska Author-Workplace-Name: Department of Plant Biology, Mendel University of Agriculture and Forestry, Brno, Czech Republic Author-Workplace-Name: Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno, Czech Republic Author-Name: O. Zítka Author-Workplace-Name: Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno, Czech Republic Author-Workplace-Name: Department of Animal Nutrition, Mendel University of Agriculture and Forestry, Brno, Czech Republic Author-Name: V. Adam Author-Workplace-Name: Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno, Czech Republic Author-Workplace-Name: Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic Author-Name: M. Beklová Author-Workplace-Name: Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Brno, Czech Republic Author-Name: S. Křížková Author-Workplace-Name: Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno, Czech Republic Author-Workplace-Name: Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic Author-Name: L. Zeman Author-Workplace-Name: Department of Veterinary Ecology and Environment Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: A. Horna Author-Workplace-Name: Department of Food Engineering and Chemistry, Faculty of Technology, Tomas Bata University, Zlín, Czech Republic Author-Name: L. Havel Author-Workplace-Name: Department of Plant Biology, Mendel University of Agriculture and Forestry, Brno, Czech Republic Author-Name: J. Zehnálek Author-Workplace-Name: Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno, Czech Republic Author-Name: R. Kizek Author-Workplace-Name: Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno, Czech Republic Title: A sensor for investigating the interaction between biologically important heavy metals and glutathione Abstract: Glutathione (GSH) is a water-soluble tripeptide playing a crucial role in the number of cellular reactions including detoxification of heavy metals. Glutathione can be found both in an oxidized (GSSG) and reduced (GSH) state. The aim of this paper was to use electrochemical methods to determine glutathiones and to investigate interactions of GSH and cadmium, zinc, copper and nickel ions or feed additive for animal nutrition. It clearly follows from the results that GSH and GSSG can be distinguished by cyclic voltammetry whereas the level of their current response is proportional to their concentration. Moreover, we studied these interactions by cyclic voltammetry. Marked changes in the GSH signal were observed and discussed. The highest decrease in a GSH reductive signal is caused by cadmium ions followed by zinc, nickel and copper ones. We also observed that the reductive GSH signal gradually decreased (about 9%) with the increasing concentration of feed additive used in our experiments. In conclusion, cyclic voltammetry offers a tool for studying interactions of thiols with different substances such as heavy metals and/or feed additives. Keywords: Keywords: reduced glutathione (GSH), oxidized glutathione (GSSG), thiols, cadmium, zinc, copper, nickel, additive, cyclic voltammetry, electrochemical metod Journal: Czech Journal of Animal Science Pages: 37-43 Volume: 52 Issue: 2 Year: 2007 DOI: 10.17221/2345-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2345-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200702-0002.txt Handle: RePEc:caa:jnlcjs:v:52:y:2007:i:2:id:2345-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: J. Čeřovský Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: S. Frydrychová Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: A. Lustyková Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: M. Rozkot Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Title: Relationship between abnormal spermatozoa and seminal plasma free amino acids in boars Abstract: The objective of this study was to provide some data on concentrations of seminal plasma free amino acids (FAAs) in order to compare these values with different total contents of morphologically abnormal spermatozoa (MAS) in the semen of 37 experimental adult boars. The boars were kept in the same housing, feeding and breeding conditions. Immediately after collection, semen gel free volume, sperm concentration and total MAS were determined microscopically. The boars were divided into two groups (A - n = 24, B - n = 13) according to the significantly different total MAS content (x: A = 9.46 ± 4.95, B = 46.00 ± 14.54%, P < 0.01). Deproteinized seminal plasma samples (supernatant) were analysed for concentrations of 13 FAAs: Tau, Asp, Thr, Ser, Glu, Gly, Ala, Val, Met, Ile, Leu, Tyr and Lys by liquid chromatography (AAA 339 M analyser). A highly significant correlation was determined between total MAS and total FAA content in all experimental boars (n = 37, r = -0.60, P < 0.01). Glutamic acid was a predominant FAA in seminal plasma in both groups of boars (x: A = 28.49 vs. B = 17.60μM/100 ml) but its concentration was significantly higher in group A (P < 0.01). The proportion (%) of glutamic acid concentration in the total content of FAAs in both groups of boars was nearly equal (A = 38.1 vs. B = 38.9%, P > 0.05) as well as the proportion index of the individual FAAs calculated from glutamic acid (= 100%). The differences in the particular FAAs recorded between group A and group B were statistically significant for 11 out of the 13 FAAs observed (P < 0.05 and P < 0.01) in favour of group A as well as in the total calculated content of FAAs per boar (x: 74.70 vs. 45.23μM/100 ml, P < 0.01). A significantly negative relationship between the MAS content and the concentration of seminal plasma FAAs (r = -0.60, P < 0.01) is the main result of this study with regard to the markers for potential boar semen fertility estimation. Keywords: boars, semen, morphologically abnormal spermatozoa, seminal plasma free amino acids Journal: Czech Journal of Animal Science Pages: 44-49 Volume: 52 Issue: 2 Year: 2007 DOI: 10.17221/2346-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2346-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200702-0003.txt Handle: RePEc:caa:jnlcjs:v:52:y:2007:i:2:id:2346-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: R. Polat Author-Workplace-Name: Department of Agricultural Machinery, Faculty of Agriculture, Harran University, Sanliurfa, Turkey Author-Name: S. Tarhan Author-Workplace-Name: Department of Agricultural Machinery, Faculty of Agriculture, Gaziosmanpasa University, Tokat, Turkey Author-Name: M. Çetin Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, Harran University, Sanliurfa, Turkey Author-Name: U. Atay Author-Workplace-Name: Department of Agricultural Machinery, Faculty of Agriculture, Harran University, Sanliurfa, Turkey Title: Mechanical behaviour under compression loading and some physical parameters of Japanese quail (Coturnix coturnix japonica) eggs Abstract: Technical information and data on the physical and mechanical properties of agricultural and animal products are necessary to design various equipments for agriculture and industry. In this study some physical properties such as mass, length, diameter, geometric mean diameter, surface area, sphericity, volume, coefficient of friction and packaging coefficient were determined for Japanese quail eggs. Furthermore, the mechanical behaviour of Japanese quail eggs was determined in terms of average rupture force, deformation and toughness (energy absorbed by the Japanese quail eggs per unit volume). Egg samples were compressed along their X and Z-axes. The average values of their mass, length, width, shell thickness, geometric mean diameter, surface area, sphericity, volume and packaging coefficient were measured to be 12.69 g, 34.87 mm, 26.20 mm, 0.27 mm, 28.82 mm, 2 608.5 mm2, 1.10, 359.17 mm3, 0.469, respectively. The values of the coefficient of friction for quail eggs on the surfaces of plywood, glass, galvanized steel and fibreglass were 0.301, 0.282, 0.274 and 0.266, respectively. The highest rupture force, deformation and toughness were obtained when Japanese quail eggs were loaded along their X-axis. Compression along the Z-axis required the least compressive force to break the eggs as compared to the other compression axes. Rupture force, deformation, absorbed energy and toughness for the X-front axis were determined to be 10.51 N, 1.5 mm, 7.88 Nmm and 0.219 Mj/mm2, respectively. Keywords: quail egg, physical properties, mechanical behaviour Journal: Czech Journal of Animal Science Pages: 50-56 Volume: 52 Issue: 2 Year: 2007 DOI: 10.17221/2262-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2262-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200702-0004.txt Handle: RePEc:caa:jnlcjs:v:52:y:2007:i:2:id:2262-CJAS