Template-Type: ReDIF-Article 1.0 Author-Name: A. Matějíček Author-Workplace-Name: University of South Bohemia, Ceske Budejovice, Czech Republic Author-Name: J. Matějíčková Author-Workplace-Name: Institute of Animal Science, Prague-Uhrineves, Czech Republic Author-Name: E. Němcová Author-Workplace-Name: Institute of Animal Science, Prague-Uhrineves, Czech Republic Author-Name: O.M. Jandurová Author-Workplace-Name: Crop Research Institute, Prague, Czech Republic Author-Name: M. Štípková Author-Workplace-Name: Institute of Animal Science, Prague-Uhrineves, Czech Republic Author-Name: J. Bouška Author-Workplace-Name: Institute of Animal Science, Prague-Uhrineves, Czech Republic Author-Name: J. Frelich Author-Workplace-Name: University of South Bohemia, Ceske Budejovice, Czech Republic Title: Joint effects of CSN3 and LGB genotypes and their relation to breeding values of milk production parameters in Czech Fleckvieh Abstract: The objective of this study was to estimate the joint effects of CSN3 and LGB genotypes on breeding values of milk production parameters. CSN3 (kappa-casein) and LGB (beta-lactoglobulin) genotypes of 120 Czech Fleckvieh sires were detected using the PCR-RFLP method. Breeding values of sires were obtained from the Official Database of Progeny Testing. Ten genotype combinations were detected. Genotypes ABAB (25.0%), ABAA (13.3%) and ABBB (13.3%) were the most frequent. Significant effects of genotype combinations on breeding values for fat and protein content were found. The highest breeding values for milk (+621 kg) and protein (+15.8 kg) yields were associated with genotype combination ABAA, while the highest breeding values for content parameters (+0.15% for protein content and +0.55% for fat content) were associated with genotype combination BBAB. Keywords: cattle, kappa-casein, beta-lactoglobulin, milk production parameters, breeding values Journal: Czech Journal of Animal Science Pages: 83-87 Volume: 52 Issue: 4 Year: 2007 DOI: 10.17221/2273-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2273-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200704-0001.txt Handle: RePEc:caa:jnlcjs:v:52:y:2007:i:4:id:2273-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: M. Simon Author-Workplace-Name: Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic Author-Name: S. Hluchý Author-Workplace-Name: Slovak University of Agriculture, Nitra, Slovak Republic Author-Name: Ľ. Horovská Author-Workplace-Name: Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic Author-Name: J. Antalíková Author-Workplace-Name: Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic Author-Name: J. Čuboň Author-Workplace-Name: Slovak University of Agriculture, Nitra, Slovak Republic Title: Immunohistochemical localization of adhesion molecules (CD62 and CD18) in the mammary gland of dairy cows Abstract: Localization of the L-selectin (CD62L) and β2-integrin (CD18) bearing cells in different tissues of the bovine mammary gland was examined. Five dairy cows of Holstein-Friesian breed in the middle of their second and third lactation cycle were used in the study. Blood, milk and udder tissue samples were collected from each cow to estimate the milk somatic cell count (SCC) and bacteriological infection of the mammary gland. The expression of CD62L and CD18 on blood cells, milk cells and parenchymal tissues of udder, Fürstenberg's rosette and the transverse section of the central part of the teat was tested. In the mammary gland quarters the value of SCC in milk secretion was also reflected in the presence of CD18+ and CD62L+ leukocytes in mammary tissues. In the quarters where SCC was higher than 105 a frequent incidence of L-selectin and β2-integrin cells was observed in the parenchyma and Fürstenberg's rosette region, while in the quarters with low SCC, none or only a few reactive cells were found. In the mammary parenchymal tissue CD18 positive cells were present in both the epithelial and the connective tissue. In Fürstenberg's rosette the cells were concentrated in the connective collagenous and loose tissue. None or only scattered L-selectin (CD62L) and β2-integrin (CD18) bearing cells were identified in the transverse section from the central part of the teat. When we compared the L-selectin and β2-integrin expression, the study revealed the down-regulation of L-selectin on the cells of mammary tissue. Keywords: cattle, L-selectin, β 2-integrin, mastitis Journal: Czech Journal of Animal Science Pages: 88-95 Volume: 52 Issue: 4 Year: 2007 DOI: 10.17221/2272-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2272-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200704-0002.txt Handle: RePEc:caa:jnlcjs:v:52:y:2007:i:4:id:2272-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: V. Vajda Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Author-Name: I. Maskaľová Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Author-Name: A. Tesfaye Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Title: Acid-base homeostasis of blood and pH of abomasum in calves fed non-acidified and acidified milk replacer Abstract: Experiments were conducted on calves divided into three groups, 6 animals in each, to determine the influence of the intake of non-acidified and acidified milk replacer on the level of acid-base homeostasis in venous blood. The milk replacer was supplied at a dose of 700 g dry matter in 8 l of the liquid in two feedings. The milk replacer was acidified by adding formic acid to ensure the concentration of 0.2% in milk replacer. Venous blood was collected before feeding and 1, 2, 4, and 6 hours after feeding. The samples were analyzed for blood pH, actual bicarbonate HCO3- (mmol/l), base excess BE (mmol/l), partial pressure of carbon dioxide pCO2 (kPa) and partial pressure of oxygen pO2 (kPa). Significant changes were observed in calves fed acidified milk of albumin type. The mean daily values of acid-base parameters in these calves were significantly lower in comparison with animals fed non-acidified milk replacer and reached the following levels: pH 7.343 ± 0.032 (P < 0.05), HCO3 24.49 ± 2.13 mmol/l (P < 0.01), BE 1.11 ± 1.97 mmol/l (P < 0.001). A similar tendency but of a more pronounced decrease in values was recorded in the group of calves fed acidified milk replacer of casein type: pH 7.312 ± 0.022 (P < 0.01), HCO3- 21.73 ± 0.75 mmol/l (P < 0.001), BE 96 ± 0.86 mmol/l (P < 0.001). In relation to the time after feeding the group of calves fed non-acidified milk replacer showed a rising tendency in the level of metabolic components (HCO3-), compensated by respiratory regulating mechanisms (rise in pCO2) conducive to the maintenance of optimum blood pH level. The group of calves fed acidified milk replacer (formic acid 2 ml/l) of the albumin type showed metabolic acidosis with subsequent gradual adjustment and compensation by means of metabolic (HCOHCO3-) rather than respiratory regulation mechanisms. The calves fed acidified casein type milk replacer displayed metabolic acidosis with insufficient metabolic regulation and more intensive respiratory compensation (decrease in pCO2). Concurrent investigations of the abomasum acidity and blood acid-base homeostasis reflected the joint action of both the acidifying effect of formic acid and significantly lower production of bicarbonate (HCO3-) related to the intake of acidified milk and the tendency to the development of metabolic acidosis. Keywords: calves, milk feeding, formic acid, acid-base balance, pH of abomasums Journal: Czech Journal of Animal Science Pages: 96-102 Volume: 52 Issue: 4 Year: 2007 DOI: 10.17221/2271-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2271-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200704-0003.txt Handle: RePEc:caa:jnlcjs:v:52:y:2007:i:4:id:2271-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: D. Škorjanc Author-Workplace-Name: Faculty of Agriculture, University of Maribor, Slovenia Author-Name: A. Šalehar Author-Workplace-Name: Zootechnical Department, Biotechnical Faculty, University of Ljubljana, Slovenia Author-Name: I. Eržen Author-Workplace-Name: Medical Faculty, Institute of Anatomy, University of Ljubljana, Slovenia Author-Name: M. Kastelic Author-Workplace-Name: Zootechnical Department, Biotechnical Faculty, University of Ljubljana, Slovenia Title: Pattern of fibre type distribution within muscle fascicles of pigs (Sus scrofa domestica) Abstract: In order to determine breed differences in the fascicle structure of skeletal muscles, longissimus dorsi muscles (LD) and semimembranous muscles (SM), Duroc (D), Large White (LW), German Landrace (GL) and Swedish Landrace (SL) boars were analyzed. Fibre type delineation was based on the method of myofibrillar ATPase staining. The fibres in a fascicle were divided into layers according to their relation to the perimysium. In each layer, the proportions of fibre types I, IIA and IIB were determined and normalized according to the fibre type proportion in the whole fascicle. Generally, a high proportion of IIB fibres on the periphery, a prevalence of type I and IIA in the layer below, and altered proportions of type I, IIA and IIB in layer 3 were found to be the main characteristics of the distribution of fibres in a porcine fascicle. Breed dissimilarity was established in the arrangement of fibre types, particularly in the first and third layer. The breeds that showed the highest proportions of type I and IIB fibres in LD and SM muscles were GL and SL boars. Keywords: pig, skeletal muscle, fascicle, muscle fibre types, spatial distribution Journal: Czech Journal of Animal Science Pages: 103-109 Volume: 52 Issue: 4 Year: 2007 DOI: 10.17221/2270-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2270-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200704-0004.txt Handle: RePEc:caa:jnlcjs:v:52:y:2007:i:4:id:2270-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Ľ. Zemková Author-Workplace-Name: Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno, Czech Republic Author-Name: J. Simeonovová Author-Workplace-Name: Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno, Czech Republic Author-Name: M. Lichovníková Author-Workplace-Name: Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno, Czech Republic Author-Name: K. Somerlíková Author-Workplace-Name: Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno, Czech Republic Title: The effects of housing systems and age of hens on the weight and cholesterol concentration of the egg Abstract: The effects of laying hen age and the housing of laying hens in conventional cages, enriched cages, litter system and outdoor system on egg weight and yolk and egg cholesterol concentration were studied. ISA brown laying hens were used in the experiment. The characteristics were measured at 39, 50, 59, 68 and 75 weeks of age. Fifteen eggs were always collected from each housing system. In total, 300 eggs were analyzed. There were not any significantly different results in the eggs from the hens living in the different housing systems or from the age of laying hens on the weight of eggs. But the housing system and the age of hens had a significant (P ≤ 0.01) effect on both yolk and egg cholesterol concentration. The concentration of yolk and egg cholesterol was the lowest (P ≤ 0.01) in the enriched cages (12.5 mg/g yolk and 211.2 mg/egg) and the highest in the litter technology (14.1 mg/g yolk and 242.6 mg/egg). Further, there was a significant (P ≤ 0.01) influence of the interaction between the housing system and the age of hens on egg weight and on yolk and egg cholesterol concentration. Keywords: conventional cages, enriched cages, litter system, outdoor system, yolk cholesterol, egg weight Journal: Czech Journal of Animal Science Pages: 110-115 Volume: 52 Issue: 4 Year: 2007 DOI: 10.17221/2269-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2269-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200704-0005.txt Handle: RePEc:caa:jnlcjs:v:52:y:2007:i:4:id:2269-CJAS