Template-Type: ReDIF-Article 1.0 Author-Name: B. Hučko Author-Workplace-Name: Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences, Prague, Czech Republic Author-Name: V.A. Bampidis Author-Workplace-Name: Department of Animal Production, School of Agricultural Technology, Alexander Technological Educational Institute, Thessaloniki, Greece Author-Name: A. Kodeš Author-Workplace-Name: Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences, Prague, Czech Republic Author-Name: V. Christodoulou Author-Workplace-Name: Animal Research Institute, National Agricultural Research Foundation, Giannitsa, Greece Author-Name: Z. Mudřik Author-Workplace-Name: Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences, Prague, Czech Republic Author-Name: K. Poláková Author-Workplace-Name: Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences, Prague, Czech Republic Author-Name: V. Plachý Author-Workplace-Name: Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences, Prague, Czech Republic Title: Rumen fermentation characteristics in pre-weaning calves receiving yeast culture supplements Abstract: In an experiment with 45 neonatal male Holstein calves, effects of yeast cultures Yea-Sacc® 1026 (SC) containing Saccharomyces cerevisiae (strain 1026) and Vitex (KF) containing Kluyveromyces fragilis (strain Jürgensen) on rumen fermentation characteristics were determined. From day 4 to day 56 of age, the calves were allocated to one of the three dietary treatments (Control, SC, and KF) of 15 animals each, placed in individual pens, and fed 4 l of whole milk twice daily and a basal concentrate mixture ad libitum as calf starter feeds. The control treatment was not supplemented with yeast culture. The yeast culture supplements Yea-Sacc® 1026 and Vitex were top-dressed at 10 g/calf daily on the basal concentrate mixture of treatments SC and KF, respectively. At the end of the experiment (day 56), all calves were slaughtered and the rumen fluid was analysed. The administration of yeast cultures Yea-Sacc®026 and Vitex to calves did not affect final body weight (BW), BW gain, dry matter intake, feed conversion ratio, ruminal pH, lactic acid concentration and the molar proportion of propionic acid, but it decreased (P < 0.05) the total volatile fatty acid concentration and the molar proportion of butyric acid, and increased (P < 0.05) the molar proportion of acetic acid and the acetate to propionate ratio. In addition, the microbial cellulolytic activity was higher in calves that received both yeast cultures compared to the control treatment. The results of this study suggest that the ruminal fermentation was more stable in calves receiving yeast culture supplements. Keywords: Holstein calves, Saccharomyces cerevisiae, Kluyveromyces fragilis, performance, ruminal pH, volatile fatty acids, lactic acid, microbial cellulolytic activity Journal: Czech Journal of Animal Science Pages: 435-442 Volume: 54 Issue: 10 Year: 2009 DOI: 10.17221/1674-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/1674-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200910-0001.txt Handle: RePEc:caa:jnlcjs:v:54:y:2009:i:10:id:1674-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: J. Zrůstová Author-Workplace-Name: Department of Animal Morphology, Physiology and Genetics, Mendel University of Agriculture and Forestry in Brno, Brno, Czech Republic Author-Name: A. Knoll Author-Workplace-Name: Department of Animal Morphology, Physiology and Genetics, Mendel University of Agriculture and Forestry in Brno, Brno, Czech Republic Author-Workplace-Name: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic Author-Name: T. Urban Author-Workplace-Name: Department of Animal Morphology, Physiology and Genetics, Mendel University of Agriculture and Forestry in Brno, Brno, Czech Republic Author-Name: S. Čepica Author-Workplace-Name: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic Title: The visfatin (NAMPT; PBEF1) gene polymorphisms and associations with meat performance traits in three pig breeds kept in the Czech Republic Abstract: Visfatin encoded by the NAMPT gene (nicotinamide phosphoribosyltransferase, formerly also known as PBEF - pre-B cell colony-enhancing factor) is suggested to play a role in lipid metabolism and pathophysiology of diabetes mellitus type 2. A new microsatellite SCZ004 was detected within intron 9 of the NAMPT gene. In Czech Large White (n = 95) frequencies of alleles 282, 286, 287, 299, and 304 were 0.02, 0.39, 0.07, 0.04, and 0.48, respectively. Allele 286 was predominant also in Landrace (n = 11) and Black Pied Prestice (n = 11) breeds. Association analysis was carried out between previously reported SNP AM999341:g.669T>C in intron 9 of the NAMPT gene and backfat thickness, average daily gain and lean meat content in Czech Large White (n = 215), Black Pied Prestice (n = 96) and Landrace (n = 105). The CC genotype was associated with higher backfat thickness ( Keywords: visfatin, NAMPT, PBEF, SNP, microsatellite, pig, association analysis Journal: Czech Journal of Animal Science Pages: 443-447 Volume: 54 Issue: 10 Year: 2009 DOI: 10.17221/1673-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/1673-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200910-0002.txt Handle: RePEc:caa:jnlcjs:v:54:y:2009:i:10:id:1673-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Y. Tao Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, China Author-Name: W. Han Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, China Author-Workplace-Name: Reproduction Centre, Reproduction and Genetics Institute of Chongqing, Chongqing, China Author-Name: M. Zhang Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, China Author-Name: Y. Zhang Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, China Author-Name: J. Fang Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, China Author-Name: J. Liu Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, China Author-Name: R. Zhang Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, China Author-Name: H. Chen Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, China Author-Name: F. Fang Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, China Author-Name: N. Tian Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, China Author-Name: D. Huo Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, China Author-Name: Y. Liu Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, China Author-Name: F. Li Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, China Author-Name: J. Ding Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, China Author-Name: P. Maddox-Hyttel Author-Workplace-Name: Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark Author-Name: X. Zhang Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, China Title: Production of Boer goat (Capra hircus) by nuclear transfer of cultured and cryopreserved fibroblast cells into slaughterhouse-derived oocytes Abstract: The present study reports a birth of goat clone produced by nuclear transfer of cultured and cryopreserved fibroblast cells into slaughterhouse-derived oocytes. The donors of somatic cells were fibroblasts derived from the ear skin of a Boer goat while the recipient ooplasm was in vitro matured oocytes of Huanghuai white goat, an Anhui native goat species. The reconstructed embryos were cultured in vitro and then the morphologically normal embryos were transferred to the surrogates. The reconstructed embryos were surgically transferred into 37 recipient surrogates, Huanghuai white goats with natural oestrus. Five of them were treated with hCG after transfer. Among them, one was pregnant and gave birth to a live kid. Due to the improper delivery aid, the cloned kid died accidentally after birth. The cloned kid was then anatomised to observe the viscera development, and the results showed that the organs were normal. Paraffin tissue slices were prepared and stained to ensure the viscera development further, and the results suggested that the organs also developed well in spite of incipient hydropericardium. The microsatellite analysis identified the cloning. It is suggested that the optimised nuclear transfer protocol and proper hCG treatment lead to the successful birth of a goat clone. Keywords: goat, fibroblast, clone, hCG Journal: Czech Journal of Animal Science Pages: 448-460 Volume: 54 Issue: 10 Year: 2009 DOI: 10.17221/1672-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/1672-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200910-0003.txt Handle: RePEc:caa:jnlcjs:v:54:y:2009:i:10:id:1672-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: R.J. Chen Author-Workplace-Name: Animal Science and Technology College, Yangzhou University, Yangzhou, China Author-Name: Z.P. Yang Author-Workplace-Name: Animal Science and Technology College, Yangzhou University, Yangzhou, China Author-Name: D.J. Ji Author-Workplace-Name: Animal Science and Technology College, Yangzhou University, Yangzhou, China Author-Name: D.Y. Qu Author-Workplace-Name: Animal Science and Technology College, Yangzhou University, Yangzhou, China Author-Name: Y.L. Li Author-Workplace-Name: Animal Science and Technology College, Yangzhou University, Yangzhou, China Author-Name: Y.J. Mao Author-Workplace-Name: Animal Science and Technology College, Yangzhou University, Yangzhou, China Author-Name: D.L. Huang Author-Workplace-Name: Animal Science and Technology College, Yangzhou University, Yangzhou, China Title: Analysis of genetic polymorphism in six meat sheep breeds and genetic distances between them Abstract: The genomes of 6 sheep populations (Dorset, Texel, Black-Suffolk, Tan sheep, Small-tailed Han sheep, Tan and Small-tailed Han hybrid sheep) were screened using 7 microsatellite DNA markers to estimate the genetic diversities and genetic distances among these populations. About 105 alleles were detected at 7 loci in 6 populations. The average observed and expected heterozygosity ranged from 0.2901 to 0.4534 and from 0.8007 to 0.8737 in 6 sheep populations. The expected heterozygosity of each population was much higher than the observed heterozygosity. The mean polymorphism information content (PIC) value of populations ranged from 0.7188 to 0.8546. The coefficient of gene differentiation (Fst) between populations was very low (6.98%). The percentage of inbreeding coefficient for all populations (Fit) was 56.02%, while within breeds (Fis) it was 52.72%. Assuming that heterosis could be estimated on the basis of genetic distances between examined breeds, the hybrid of Tan and Small-tailed Han sheep could be designated as the best female parent, followed by Tan sheep and Small-tailed Han sheep. Keywords: sheep, microsatellite DNA, genetic polymorphism, heterosis Journal: Czech Journal of Animal Science Pages: 461-467 Volume: 54 Issue: 10 Year: 2009 DOI: 10.17221/1671-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/1671-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200910-0004.txt Handle: RePEc:caa:jnlcjs:v:54:y:2009:i:10:id:1671-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: S. Kusza Author-Workplace-Name: Institute of Animal Science, University of Debrecen, Centre of Agriculture Sciences and Engineering, Debrecen, Hungary Author-Name: E. Gyarmathy Author-Workplace-Name: Slovak University of Agriculture, Nitra, Slovak Republic Author-Name: J. Dubravska Author-Workplace-Name: Ministry of Agriculture of the Slovak Republic, Bratislava, Slovak Republic Author-Name: I. Nagy Author-Workplace-Name: Agricultural Biotechnology Centre, Gödöllö, Hungary Author-Name: A. Jávor Author-Workplace-Name: Institute of Animal Science, University of Debrecen, Centre of Agriculture Sciences and Engineering, Debrecen, Hungary Author-Name: S. Kukovics Author-Workplace-Name: Research Institute for Animal Breeding and Nutrition, Herceghalom, Hungary Title: Study of genetic differences among Slovak Tsigai populations using microsatellite markers Abstract: In this study genetic diversity, population structure and genetic relationships of Tsigai populations in Slovakia were investigated using microsatellite markers. Altogether 195 animals from 12 populations were genotyped for 16 microsatellites. 212 alleles were detected on the loci. The number of identified alleles per locus ranged from 11 to 35. In the majority of the populations heterozygosity deficiency and potential risks of inbreeding could be determined. High values of FST (0.133) across all the loci revealed a substantial degree of population differentiation. The estimation of genetic distance value showed that the Slovak Vojin population was the most different from the other populations. The 12 examined populations were able to group into 4 clusters. With this result our aim is to help the Slovak sheep breeders to establish their own mating system, to avoid genetic loss and to prevent diversity of Tsigai breed in Slovakia. Keywords: Slovak Tsigai sheep, microsatellite markers, genetic relationship Journal: Czech Journal of Animal Science Pages: 468-474 Volume: 54 Issue: 10 Year: 2009 DOI: 10.17221/1670-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/1670-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-200910-0005.txt Handle: RePEc:caa:jnlcjs:v:54:y:2009:i:10:id:1670-CJAS