Template-Type: ReDIF-Article 1.0 Author-Name: L. Houška Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: M. Wolfová Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: I. Nagy Author-Workplace-Name: University of Kaposvár, Kaposvár, Hungary Author-Name: Z. Csörnyei Author-Workplace-Name: Agricultural Agency of Administration, Budapest, Hungary Author-Name: I. Komlósi Author-Workplace-Name: University of Debrecen, Debrecen, Hungary Title: Economic values for traits of pigs in Hungary Abstract: Marginal economic values for production and reproduction traits of pigs were estimated applying a bio-economic model to Hungarian commercial sow herds with integrated fattening of piglets. Data collected between 2002 and 2008 were used for the calculation. Marginal economic values (in € per unit of the trait per sow per year) estimated for fattening days in the station test, age at the end of the field test, lean meat percentage in the carcass, percentage of valuable cuts in the carcass and the number of piglets born alive were 2.84 €/day, 2.71 €/day, 22.45 €/%, 28.81 €/% and 54.22 €/piglet in the first litter, respectively. Keywords: pig, breeding, economic values, bio-economic model Journal: Czech Journal of Animal Science Pages: 139-148 Volume: 55 Issue: 4 Year: 2010 DOI: 10.17221/100/2009-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/100/2009-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201004-0001.txt Handle: RePEc:caa:jnlcjs:v:55:y:2010:i:4:id:100-2009-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: M. Škrlep Author-Workplace-Name: Agricultural Institute of Slovenia, Ljubljana, Slovenia Author-Name: T. Kavar Author-Workplace-Name: Agricultural Institute of Slovenia, Ljubljana, Slovenia Author-Name: M. Čandek-Potokar Author-Workplace-Name: Agricultural Institute of Slovenia, Ljubljana, Slovenia Title: Comparison of PRKAG3 and RYR1 gene effect on carcass traits and meat quality in Slovenian commercial pigs Abstract: The effect of polymorphisms at PRKAG3 (R200Q and I199V) and RYR1 (R615C) genes on carcass traits and meat quality was examined in a sample of 257 commercial pigs, crosses of Landrace × Large White as maternal line and Pietrain (N = 96), Pietrain × Landrace (N = 42) or Pietrain × Hampshire (N = 119) as paternal line. Pigs were genotyped (PCR-RFLP) and traits of interest were measured (which included carcass and ham weight, measurements of fatness, meatiness, ultimate pH, colour parameters and drip loss). The observed genotype frequencies at PRKAG3 gene were 9.7%, 38.9%, 32.7%, 6.2% and 12.5% for R/R-I/I, R/R-I/V, R/R-V/V, Q/R-I/V and Q/R-V/V genotype, respectively. RYR1 genotype frequencies were 57.2% for N/N and 42.8% for N/n genotype. Studied polymorphisms exhibited a significant effect on meat quality, but mainly an insignificant effect on carcass traits. No significant interaction between PRKAG3 and RYR1 was found. Carriers of RYR1 mutant allele "n" had less intense longissimus dorsi muscle colour (subjective score, Minolta L* and b*) and higher drip loss. Regarding PRKAG3, the ultimate pH decreased and Minolta L*, a*, b* and drip loss increased in the following order: R/R-I/I, R/R-I/V, R/R-V/V, Q/R-I/V and Q/R-V/V, according to the presence of 199I and absence of 200Q alleles. The study shows that the I199V polymorphism is an important source of variation in pigs free of 200Q. In particular the 199I proves beneficial for meat quality. The results of combining the RYR1 and PRKAG3 genotypes indicate that R/R-I/I genotype could be used in counterbalancing the negative effects of "n" allele on meat quality. Keywords: PRKAG3, RYR1, genotype frequencies, carcass properties, meat quality, gene interaction Journal: Czech Journal of Animal Science Pages: 149-159 Volume: 55 Issue: 4 Year: 2010 DOI: 10.17221/6/2009-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/6/2009-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201004-0002.txt Handle: RePEc:caa:jnlcjs:v:55:y:2010:i:4:id:6-2009-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: S. Frydrychová Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: J. Čeřovský Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: A. Lustyková Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: M. Rozkot Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Title: Effects of long-term liquid commercial semen extender and storage time on the membrane quality of boar semen Abstract: The objective of this study was to assess the sperm membrane integrity in extended boar semen during storage time using specific spectrum laboratory methods. Boar semen was diluted with the long-term liquid commercial extenders Androhep (A), Androstar (AS), Androstar plus (AS+), LD and M III and was stored up to 96 h. The sperm membrane integrity was evaluated by motility, viable spermatozoa, short hypoosmotic swelling test (sHOST) and by the activity of the enzyme aspartate aminotransferase (AST). Negative changes in the quality of sperm membrane in relation to storage time were observed after 48 h for sHOST, after 72 h for viable spermatozoa and after 72 h for motility. The percentage of viable spermatozoa was decreased by 0.27% each hour. A statistically significant difference between extenders A and LD was observed in sHOST after 72 h and 96 h storage (P < 0.05). The AST activity did not show any statistically significant differences in extenders and in storage time. In overall assessment Androhep was the best of the tested extenders, followed by AS, AS+, M III and LD in terms of motility, viable spermatozoa and sHOST. The correlations among laboratory methods were highly significant (P < 0.001). In conclusion, the results documented that the sperm membrane integrity was statistically significantly affected by extenders and storage time (P < 0.001). Boar semen quality was the best in extender A. sHOST is a very sensitive and relatively simple method for the assessment of sperm membrane integrity in diluted semen. Keywords: boar semen, long-term liquid extender, storage time, membrane integrity Journal: Czech Journal of Animal Science Pages: 160-166 Volume: 55 Issue: 4 Year: 2010 DOI: 10.17221/62/2009-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/62/2009-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201004-0003.txt Handle: RePEc:caa:jnlcjs:v:55:y:2010:i:4:id:62-2009-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: M. Skřivan Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: G. Dlouhá Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: M. Englmaierová Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: K. Červinková Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Title: Effects of different levels of dietary supplemental caprylic acid and vitamin E on performance, breast muscle vitamin E and A, and oxidative stability in broilers Abstract: The paper reports data from four experiments (EXP). Broiler cockerels Ross 308 were allocated to 3 or 5 (EXP 4) dietary treatments comprising 300 (3 replicated pens of 100 chicks per pen), 50, 50 or 100 chickens. The EXP lasted 42, 38, 42 and 38 days. All basal diets contained adequate ingredients. The inclusion of 0.5% caprylic acid (CA) in diets reduced (P < 0.05) body weight (EXP 1). The results of EXP 3 and 4 indicated that the diet supplemented with 0.25% CA and 30 mg vitamin E (total vitamin E concentration 50 mg) resulted in similar BW like the basal diet, but the increased supplemental vitamin E to 150 mg (EXP 3) or to 100 mg (EXP 4) decreased (P < 0.05 and P < 0.001) BW and increased mortality. No differences were found between the fat and crude protein contents in dry matter of breast meat. Higher vitamin E doses in feed mixture significantly (P < 0.001) increased the content of vitamins soluble in fats in breast meat - vitamin E (from 28.54 in the control to 80.28 mg/kg of dry matter) and vitamin A (from 0.34 to 0.44 mg/kg of dry matter). The addition of caprylic acid significantly decreased the speed of lipid oxidation measured after 3 (P < 0.02) and 5 (P < 0.05) days of storage in refrigerator at a temperature from 2.5 to 4°C. On the contrary, higher vitamin E doses and basal diet without CA supplement increased the oxidation of lipids. Keywords: caprylic acid, vitamin E, meat quality, performance, male broiler Journal: Czech Journal of Animal Science Pages: 167-173 Volume: 55 Issue: 4 Year: 2010 DOI: 10.17221/221/2009-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/221/2009-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201004-0004.txt Handle: RePEc:caa:jnlcjs:v:55:y:2010:i:4:id:221-2009-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: S.M.H. Alavi Author-Workplace-Name: University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, Vodňany, Czech Republic Author-Name: M. Rodina Author-Workplace-Name: University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, Vodňany, Czech Republic Author-Name: A. Hatef Author-Workplace-Name: University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, Vodňany, Czech Republic Author-Name: V. Stejskal Author-Workplace-Name: University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, Vodňany, Czech Republic Author-Name: T. Policar Author-Workplace-Name: University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, Vodňany, Czech Republic Author-Name: J. Hamáčková Author-Workplace-Name: University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, Vodňany, Czech Republic Author-Name: O. Linhart Author-Workplace-Name: University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, Vodňany, Czech Republic Title: Sperm motility and monthly variations of semen characteristics in Perca fluviatilis (Teleostei: Percidae) Abstract: Dynamics of sperm motility (sperm velocity, percentage of motility and flagellar beat frequency) and monthly variations of semen characteristics (semen volume and osmolality and sperm concentration and motility) were studied in Perca fluviatilis. This study showed that sperm velocity, percentage of motility and flagellar beat frequency significantly and rapidly decreased after the activation of sperm motility. Twelve spermiating males were randomly selected and electronically tagged to study monthly variations of semen characteristics. The semen was collected 4 times (29th November 2005, 10th January 2006, 21st February 2006, and 7th April 2006). Semen volume did not change significantly from November to February, but it significantly increased in April. Sperm concentration was higher in November and January than in February and April. The highest and the lowest osmolality of semen was observed in January and April while it decreased in February and April. At 15 s post activation, the lowest percentage of motile spermatozoa was observed in November. The semen samples collected in April showed the lowest motility of spermatozoa (24.3%) at 30 s post activation. But the percentage of motile spermatozoa collected from November to February showed more than 65% motility at 30 s post activation. At the end of motility period (60 s post activation), no motile spermatozoa were observed in April, and the highest motility was in November. Sperm velocity did not show any significant differences at 15 s post activation. The lowest sperm velocity was observed in April at 30 s post activation. Keywords: beat frequency, semen volume, sperm concentration, osmolality, sperm motility Journal: Czech Journal of Animal Science Pages: 174-182 Volume: 55 Issue: 4 Year: 2010 DOI: 10.17221/118/2009-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/118/2009-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201004-0005.txt Handle: RePEc:caa:jnlcjs:v:55:y:2010:i:4:id:118-2009-CJAS