Template-Type: ReDIF-Article 1.0 Author-Name: G. D'Alterio Author-Workplace-Name: Department of Veterinary Clinical Science, Faculty of Veterinary Medicine, University of Padua, Padua, Italy Author-Name: S. Casella Author-Workplace-Name: Department of Experimental Science and Applied Biotechnology, Laboratory of Veterinary Chronophysiology, Faculty of Veterinary Medicine, University of Messina, Messina, Italy Author-Name: M. Gatto Author-Workplace-Name: Department of Veterinary Clinical Science, Faculty of Veterinary Medicine, University of Padua, Padua, Italy Author-Name: M. Gianesella Author-Workplace-Name: Department of Veterinary Clinical Science, Faculty of Veterinary Medicine, University of Padua, Padua, Italy Author-Name: G. Piccione Author-Workplace-Name: Department of Experimental Science and Applied Biotechnology, Laboratory of Veterinary Chronophysiology, Faculty of Veterinary Medicine, University of Messina, Messina, Italy Author-Name: M. Morgante Author-Workplace-Name: Department of Veterinary Clinical Science, Faculty of Veterinary Medicine, University of Padua, Padua, Italy Title: Circadian rhythm of foot temperature assessed using infrared thermography in sheep Abstract: The circadian rhythm of body core and surface temperature in 10 Comisana sheep kept under a natural photoperiod (06:30/19:00) was evaluated. Every 3 h for 24 consecutive hours rectal temperature (RT) and foot temperature were recorded. Particularly, foot temperature was recorded by infrared thermography, an ideal technique for evaluating the temperature not only at one point but also at the eight points as follows: in the right front of the interdigital area (FA1), in the left front of the interdigital area (FA2), in the right rear of the interdigital area (RA1), in the left rear of the interdigital area (RA2), in the right front of the interdigital line (FL1), in the left front of the interdigital line (FL2), in the right rear of the interdigital line (RL1) and in the left rear of the interdigital line (RL2). Two-way repeated measures ANOVA using SPSS, followed by Scheffé's test, showed a significant effect of the time of day and side of temperature collection (P < 0.05) on temperature values. The single cosinor procedure showed a daily rhythmicity of RT and foot temperature in all tested sides. The circadian oscillations of RT reflected the familiar circadian patterns of endogenous sources and the results of foot temperatures supported the idea that the daily rhythm was, at least in part, influenced by variation in the blood flow to the extremities. The infrared thermography providing more information on the development of disturbances in the peripheral circulation may be used with an advantage in occupational health examinations and in special clinical work. Keywords: circadian rhythm, foot temperature, infrared thermography, rectal temperature, sheep Journal: Czech Journal of Animal Science Pages: 293-300 Volume: 56 Issue: 7 Year: 2011 DOI: 10.17221/1294-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/1294-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201107-0001.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:7:id:1294-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: J. Čítek Author-Workplace-Name: Department of Genetics, Animal Breeding and Nutrition, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic Author-Name: V. Řehout Author-Workplace-Name: Department of Genetics, Animal Breeding and Nutrition, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic Author-Name: L. Hanusová Author-Workplace-Name: Department of Genetics, Animal Breeding and Nutrition, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic Author-Name: A. Míková Author-Workplace-Name: Department of Genetics, Animal Breeding and Nutrition, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic Author-Name: I. Jašková Author-Workplace-Name: Department of Genetics, Animal Breeding and Nutrition, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic Title: Polymorphisms in CGIL4, breeding value for somatic cell count and resistance to mastitis Abstract: The relationship between a potential marker for mastitis, CGIL4, and the breeding value for somatic cell count (SCC) was analysed in a panel of Czech Simmental and German Holstein sires. Genotyping was done by PCR/RFLP. The analysis did not reveal a significant difference in breeding values for SCC depending on the genotype of CGIL4, even though other authors confirmed the relationship between this marker and clinical mastitis. Further investigations will be necessary to clarify the relevance of the marker for selection against mastitis. Keywords: mastitis, CGIL4, somatic cells, Czech Simmental cattle, German Holstein cattle Journal: Czech Journal of Animal Science Pages: 301-304 Volume: 56 Issue: 7 Year: 2011 DOI: 10.17221/1297-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/1297-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201107-0002.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:7:id:1297-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Y.X. Wang Author-Workplace-Name: Feed Science Institute, College of Animal Science, Zhejiang University, Hangzhou, P.R. China Author-Name: X.A. Zhan Author-Workplace-Name: Feed Science Institute, College of Animal Science, Zhejiang University, Hangzhou, P.R. China Author-Name: D. Yuan Author-Workplace-Name: Feed Science Institute, College of Animal Science, Zhejiang University, Hangzhou, P.R. China Author-Name: X.W. Zhang Author-Workplace-Name: Feed Science Institute, College of Animal Science, Zhejiang University, Hangzhou, P.R. China Author-Name: R.J. Wu Author-Workplace-Name: Feed Science Institute, College of Animal Science, Zhejiang University, Hangzhou, P.R. China Title: Effects of selenomethionine and sodium selenite supplementation on meat quality, selenium distribution and antioxidant status in broilers Abstract: This study was conducted to investigate the effects of selenomethionine (Se-Met) and sodium selenite (SS) supplementation on meat quality, selenium (Se) distribution, and antioxidant status in broilers. A total of 360 seven-days-old Ross 308 broilers of an average body weight 162 ± 0.59 g were randomly allotted to three treatments, each of which included three replications of 40 birds. The treatments included a control diet containing 0.04 mg Se/kg and other two diets that contained 0.15 mg Se/kg supplemented by SS or Se-Met. The experiment lasted for 42 days. Selenium supplementation improved (P < 0.05) the Hunter a value of breast muscle in 8 and 16 h and decreased (P < 0.05) the drip loss of breast muscle in 24 and 48 h. Both Se sources and exposed time significantly influenced (P < 0.01) the drip loss of breast muscle. Selenium and glutathione concentrations in serum and in the studied organs were significantly higher (P < 0.05) after dietary Se supplementation while the Se-Met group showed the highest value (P < 0.05). Glutathione peroxidase activity in serum and in the studied organs was also significantly elevated (P < 0.05) by dietary Se supplementation while SS increased the glutathione peroxidase activities in pancreas and breast muscle to a larger extent (P < 0.05) than did Se-Met. The addition of Se from either source caused a significant increase (P < 0.05) in superoxide dismutase activities in tissues (except for kidney) whereas the Se-Met group was more effective (P < 0.05) than the SS group in breast muscle. Selenium supplementation increased (P < 0.05) the total antioxidant capability in serum, liver, kidney and breast muscle while the Se-Met group proved to be more effective (P < 0.05) than the SS group except for kidney. The Se-supplemented diets had a lower (P < 0.05) malondialdehyde concentration in serum and in the studied organs but the effect was more pronounced (P < 0.05) when Se-Met was used except for the serum. These results indicated that Se-Met supplementation was more effective than SS supplementation for depositing Se in serum and tissues, enhancing the antioxidant status and reducing the drip loss of breast muscle. Keywords: broiler, selenomethionine, drip loss, Hunter a value, antioxidant status Journal: Czech Journal of Animal Science Pages: 305-313 Volume: 56 Issue: 7 Year: 2011 DOI: 10.17221/1296-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/1296-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201107-0003.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:7:id:1296-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: T. Ebeid Author-Workplace-Name: Department of Poultry Production, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh, Egypt Author-Name: A. Fayoud Author-Workplace-Name: Department of Poultry Production, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh, Egypt Author-Name: S. Abou El-Soud Author-Workplace-Name: Department of Poultry Production, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh, Egypt Author-Name: Y. Eid Author-Workplace-Name: Department of Poultry Production, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh, Egypt Author-Name: M. El-Habbak Author-Workplace-Name: Department of Poultry Production, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh, Egypt Title: The effect of omega-3 enriched meat production on lipid peroxidation, antioxidative status, immune response and tibia bone characteristics in Japanese quail Abstract: The objective of the present study was to research the effects of different supplemented dietary sources of n-3 polyunsaturated fatty acids (n-3 PUFA) to produce n-3 enriched meat on growth performance, meat quality, serum antioxidative status, immune response and tibia bone characteristics in Japanese quail. Three hundred 1-day-old Japanese quail chicks were randomly weighed and divided into five dietary treatments containing 0% oil (C, negative control), 2% vegetable oil (VO, positive control), 2% linseed oil (LO), 2% fish oil (FO) and a mixture of 1% linseed oil +1% fish oil (LO+FO). Body weight and feed consumption were recorded. Fatty acid profile, cholesterol content, chemical composition and physical characteristics of breast meat were determined. Serum thiobarbituric acid-reactive substances (TBARS), glutathione peroxidase (GSH-Px) activity and total antioxidant capacity were measured. No negative effects were detected in live weight, feed consumption and physical characteristics of meat. The incorporation of n-3 PUFA in the meat proved to be successful when different sources of n-3 PUFA were used. The supplementation of n-3 PUFA caused a significant decrease in TBARS values and a significant increase in both the GSH-Px activity and total antioxidant capacity. Interestingly, the inclusion of n-3 PUFA in quail diets enhanced the antibody titre and bone morphological characteristics. Therefore, it can be concluded that the inclusion of n-3 PUFA in diets at moderate levels increased the n-3PUFA content in meat, improved the antioxidative status, reduced lipid peroxidation, enhanced the antibody response and bone morphological characteristics and did not have any negative influence on physical characteristics of meat and growth performance in Japanese quail. Keywords: fish oil, linseed oil, meat, antioxidant Journal: Czech Journal of Animal Science Pages: 314-324 Volume: 56 Issue: 7 Year: 2011 DOI: 10.17221/1293-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/1293-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201107-0004.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:7:id:1293-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: T. Laho Author-Workplace-Name: Institute of Animal Physiology, Slovak Academy of Sciences, Košice, Slovak Republic Author-Name: Z. Váradyová Author-Workplace-Name: Institute of Animal Physiology, Slovak Academy of Sciences, Košice, Slovak Republic Author-Name: K. Mihaliková Author-Workplace-Name: Institute of Animal Physiology, Slovak Academy of Sciences, Košice, Slovak Republic Author-Name: S. Kišidayová Author-Workplace-Name: Institute of Animal Physiology, Slovak Academy of Sciences, Košice, Slovak Republic Author-Name: Z. Adamechová Author-Workplace-Name: Department of Biochemical Technology, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovak Republic Author-Name: M. Čertík Author-Workplace-Name: Department of Biochemical Technology, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovak Republic Author-Name: D. Jalč Author-Workplace-Name: Institute of Animal Physiology, Slovak Academy of Sciences, Košice, Slovak Republic Title: Prefermented cereals containing fungal gamma-linolenic acid and their effect on rumen metabolism in vitro Abstract: The application of Thamnidium elegans fungal strain CCF 1456 (TE) for effective utilization of various agroindustrial materials creates new perspectives for animal cereal diets enriched with microbial γ-linolenic acid (GLA). Diets consisting of lucerne hay (LH) plus prefermented cereals (wheat bran/spent malt grains, WB+TE or WB+TE enriched with sunflower oil, WB+SO+TE in the first experiment and ground maize grains, GC+TE in the second experiment) were used in the artificial rumen. We examined their effect on the rumen fermentation pattern and lipid metabolism. The diet affected the results of degradability of dry matter, organic matter, neutral detergent fibre and acid detergent fibre of LH+WB diets (P < 0.05 and P < 0.01). The GLA daily output of prefermented diet substrates LH+WB+TE and LH+WB+SO+TE, or LH+GC+TE was higher compared to the non-prefermented LH+WB or LH+GC, respectively (P < 0.05 and P < 0.01). Daily outputs of trans11 oleic (TVA) of the LH+GC+TE diet were higher versus the non-prefermented LH+GC (P < 0.01). The biohydrogenation of fatty acids (C18:1 cis9 oleic, C18:2 linoleic, C18:3n-3 alpha-linolenic, C18:3n-6 GLA and total FA) of prefermented cereal diets was not influenced. Cereal diets containing microbial GLA might positively enhance GLA daily outputs in the RUSITEC effluent, but they are not effective enough to decrease the biohydrogenation of unsaturated fatty acids. Keywords: fatty acids, fermentation, fungal strain, gamma-linolenic acid, in vitro Journal: Czech Journal of Animal Science Pages: 325-335 Volume: 56 Issue: 7 Year: 2011 DOI: 10.17221/1295-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/1295-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201107-0005.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:7:id:1295-CJAS