Template-Type: ReDIF-Article 1.0 Author-Name: F. Weisz Author-Workplace-Name: Department of Animal Morphology, Physiology and Genetics, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic Author-Workplace-Name: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic Author-Name: T. Urban Author-Workplace-Name: Department of Animal Morphology, Physiology and Genetics, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic Author-Name: P. Chalupová Author-Workplace-Name: Department of Animal Morphology, Physiology and Genetics, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic Author-Name: A. Knoll Author-Workplace-Name: Department of Animal Morphology, Physiology and Genetics, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic Title: Association analysis of seven candidate genes with performance traits in Czech Large White pigs Abstract: In this study association analyses were performed between genes tagged SNP (IGF2, NAMPT, DGAT1, MYF4, MC3R, MC4R and MYOD1), performance traits (backfat thickness, lean meat content, average daily gain from birth to the end of the test, average daily gain in test) and estimated breeding values (EBVs) in a population of Czech Large White sows (n = 101). Genotyping of all SNPs was performed by SNapShot with the exception of SNP within NAMPT gene for which HpaII PCR-RFLP assay was used. The following significant associations between genes tagged SNPs and traits or EBVs were found out: DGAT1 - lean meat content (AG > AA, P ≤ 0.05), MC4R - EBV for lean meat content (GG > AA, P ≤ 0.05; GG > AG, P ≤ 0.05), IGF2 - EBV for reproduction (piglets born alive in the second and subsequent parity) (AG > AA, P ≤ 0.05) and total EBV (AG > AA, P ≤ 0.01) and MC3R - EBV for average daily gain (CT > TT, P ≤ 0.05). Keywords: pig, genotyping, SNP, performance trait, estimated breeding value (EBV), Czech Large White Journal: Czech Journal of Animal Science Pages: 337-344 Volume: 56 Issue: 8 Year: 2011 DOI: 10.17221/169/2010-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/169/2010-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201108-0001.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:8:id:169-2010-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: M.M. El-Gamal Author-Workplace-Name: Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt Title: The effect of depuration on heavy metals, petroleum hydrocarbons, and microbial contamination levels in Paphia undulata (Bivalvia: Veneridae) Abstract: The depuration of 8 heavy metals (Zn, Pb, Ni, Mn, Cu, Cr, Co and Cd), total petroleum hydrocarbons and pathogenic bacteria of Paphia undulata was tested and the survival of depurated clams was evaluated. Investigated samples were collected from Ismailia, Egypt. The initial metal concentrations were significantly higher in the whole soft tissues than in water and sediment except for Mn. After 24 h depuration, Zn, Pb, Ni, Mn, Cu, Cr and Cd were significantly reduced to 44, 23, 25, 17, 61, 41, and 75%, respectively. After three days of depuration the reduction was significant only for Cu, Cr, Co and Cd (27, 15, 23 and 52%, respectively). The total petroleum hydrocarbons were reduced significantly to 72% after three days of depuration, while after 24 h they were reduced to 90% compared to their initial concentrations. Four pathogenic bacteria were identified in the soft tissues of P. undulata (Vibrio sp., Shigella sp., Escherichia coli and Salmonella sp.). After one-day depuration the results evidenced the mean microbial reduction to 75, 31, 68, and 36%, respectively, compared to their initial counts. After three days of depuration the counts of Vibrio sp. and Salmonella sp. were reduced to 3% and 8%, respectively, while Escherichia coli was not detected on the third day. Shigella sp. was increased by 22% compared to the first day of depuration. The viability and mortality were not influenced by the depurative treatment. Keywords: bivalve, depuration, heavy metals, pathogenic bacteria, petroleum hydrocarbons, survival Journal: Czech Journal of Animal Science Pages: 345-354 Volume: 56 Issue: 8 Year: 2011 DOI: 10.17221/2395-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2395-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201108-0002.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:8:id:2395-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Y. Bozkurt Author-Workplace-Name: Department of Aquaculture, Faculty of Fisheries, Mustafa Kemal University, Hatay, Turkey Author-Name: F. Öğretmen Author-Workplace-Name: State Hydraulic Works, Fish Production Station, Adana, Turkey Author-Name: Ö. Kökçü Author-Workplace-Name: Environment Conservation and National Parks Fish Production Station, Mersin, Turkey Author-Name: U. Erçin Author-Workplace-Name: Department of Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey Title: Relationships between seminal plasma composition and sperm quality parameters of the Salmo trutta macrostigma (Dumeril, 1858) semen: with emphasis on sperm motility Abstract: The mineral and organic composition of seminal plasma, physical spermatological parameters and their physiological relationships were investigated in Salmo trutta macrostigma. The seminal plasma contained 121.0 ± 0.37mM/l (Na+), 8.18 ± 0.03mM/l (K+), 7.23 ± 0.03 mg/dl (Ca2+), 3.19 ± 0.02 mEq/l (Mg++), 0.48 ± 0.02 g/dl total protein, 6.07 ± 0.06 mg/dl cholesterol, 6.24 ± 0.08 mg/dl triglyceride and 9.97 ± 0.39 mg/dl urea. The following physical spermatological parameters were found out: sperm volume 13.93 ± 0.84 ml, sperm motility 80.37 ± 2.36%, movement duration 81.47 ± 4.21 s, density 6.02 ± 0.46 × 109/ml, total density 8.85 ± 6.12 × 109 and pH 7.53 ± 0.20. Significant positive relationships were determined between motility duration and motility (r = 0.83, P < 0.01) and also between spermatocrit and motility (r = 0.536, P < 0.05). Sperm volume and total density negatively correlated with motility (r = -0.191, P > 0.05 and r = -0.087, P > 0.05, respectively). The Na+, K+ and Cl- ions correlated negatively with motility (r = -0.267, P > 0.05, r = -0.152, P > 0.05 and r = -0.461, P > 0.05, respectively). On the other hand, the Ca2+ and Mg2+ ions correlated positively with motility (r = 0.114, P > 0.05 and r = 0.040, P > 0.05, respectively). A significant negative relationship was found between motility and urea (r = -0.515, P < 0.05). These parameters should be considered when developing procedures for short-term storage or cryopreservation of the Salmo trutta macrostigma sperm. Keywords: spermatological parameters, spermatozoa, seminal fluid, Salmo trutta macrostigma Journal: Czech Journal of Animal Science Pages: 355-364 Volume: 56 Issue: 8 Year: 2011 DOI: 10.17221/2394-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2394-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201108-0003.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:8:id:2394-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: I. Nagy Author-Workplace-Name: Department of Agricultural Product Processing and Qualification, Faculty of Animal Science, Kaposvár University, Kaposvár, Hungary Author-Name: J. Farkas Author-Workplace-Name: Faculty of Economic Science, Kaposvár University, Kaposvár, Hungary Author-Name: P. Gyovai Author-Workplace-Name: Department of Agricultural Product Processing and Qualification, Faculty of Animal Science, Kaposvár University, Kaposvár, Hungary Author-Name: I. Radnai Author-Workplace-Name: Department of Agricultural Product Processing and Qualification, Faculty of Animal Science, Kaposvár University, Kaposvár, Hungary Author-Name: Z. Szendrő Author-Workplace-Name: Department of Agricultural Product Processing and Qualification, Faculty of Animal Science, Kaposvár University, Kaposvár, Hungary Title: Stability of estimated breeding values for average daily gain in Pannon White rabbits Abstract: Stability of estimated breeding values for average daily gain (ADG) between 5 and 10 weeks of age was analysed for 47 242 Pannon White rabbits, reared in 7470 litters and born between 2000 and 2008. The dataset was divided into 5 successive 5-year periods: (1) 2000-2004, (2) 2001-2005, (3) 2002-2006, (4) 2003-2007, and (5) 2004-2008. Then, after selecting the appropriate part of the pedigree for these sub-datasets, genetic parameters and breeding values were estimated for ADG using REML and BLUP methods. In the applied models sex, year-month, animal and random litter effects were considered. Estimated heritabilities for all 5 periods from 1 to 5 were moderate and stable (0.28 ± 0.01, 0.28 ± 0.02, 0.29 ± 0.02, 0.27 ± 0.02, and 0.28 ± 0.02). Magnitudes of random litter effects were low and stable (0.14 ± 0.01, 0.15 ± 0.01, 0.15 ± 0.01, 0.16 ± 0.01, and 0.16 ± 0.01). After breeding value estimation the dataset of period 5 was merged pair-wise with the other periods 4, 3, 2 and 1 using an inner join. Thus only the common records of the datasets representing the periods 5-4, 5-3, 5-2, and 5-1 were included in the merged datasets. In these merged datasets each rabbit had two breeding values for ADG based on two different periods. Spearman's rank correlation coefficients were calculated between the breeding values based on the dataset of period 5 and the other periods. With the successive years the rank correlation coefficients decreased (0.989, 0.979, 0.965 and 0.924). The correlation coefficients between ranks remained moderately high, even when the proportion of the common rabbits in the merged datasets was low. However, a reasonable re-ranking occurred among the top animals. Rank correlations for the top 100 and 1000 animals varied from 0.41 to 0.55 and from 0.37 to 0.54, respectively, which could influence selection efficiency if the rolling base were used for genetic evaluation. Keywords: sub-datasets, inner join, rank correlation Journal: Czech Journal of Animal Science Pages: 365-369 Volume: 56 Issue: 8 Year: 2011 DOI: 10.17221/2398-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2398-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201108-0004.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:8:id:2398-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: A. Abdolmohammadi Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran Author-Name: H. Atashi Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, Shiraz University, Shiraz, Iran Author-Name: P. Zamani Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran Author-Name: C. Bottema Author-Workplace-Name: Livestock Systems Alliance, University of Adelaide, Roseworthy, Australia Title: High resolution melting as an alternative method to genotype diacylglycerol O-acyltransferase 1 (DGAT1) K232A polymorphism in cattle Abstract: PCR-RFLP analysis is a common method for genotyping the DGAT1 K232A polymorphism in cattle. Our purpose was to develop a high resolution melting (HRM) assay in order to genotype the polymorphic alleles. Firstly, the PCR-RFLP method was used and the 411 bp products including the DGAT1 polymorphism were digested by CfrI enzyme. Direct sequencing was performed to confirm genotypes of the K232A polymorphism for 30 samples that presented different PCR-RFLP patterns. It was determined according to sequencing results that partial enzyme digestion had occurred for some samples. A 130 bp fragment including the polymorphism was amplified for real time PCR. Then, the HRM analysis was carried out using two fluorescent dyes, SYBR Green I and EvaGreenTM. Although the HRM genotyping using SYBR Green I was contradicted by the sequencing results, three correct melting curves were obtained for the K232A polymorphism when EvaGreenTM was used. There were no false genotypes and all genotypes were in agreement with their sequencing results. The difference in the Tm between the two homozygous groups was about 0.5°C and the AA genotypes showed a higher Tm than the KK genotypes. The heterozygous genotypes showed a different pattern. Similar results were obtained from different concentrations of EvaGreenTM in the reactions. All 206 DNA samples were genotyped using this fluorescent dye with estimated allele frequencies of 0.66 and 0.34 for the A and K alleles, respectively. Our study showed that HRM analysis will be applicable for genotyping the DGAT1 K232A polymorphism in large populations of dairy cattle. Keywords: HRM analysis, PCR-RFLP, K232A polymorphism Journal: Czech Journal of Animal Science Pages: 370-376 Volume: 56 Issue: 8 Year: 2011 DOI: 10.17221/2393-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2393-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201108-0005.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:8:id:2393-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: A. Lotfi Author-Workplace-Name: Department of Animal Science, Shabestar branch, Islamic Azad University, Shabestar, Iran Author-Name: H. Aghdam-Shahryar Author-Workplace-Name: Department of Animal Science, Shabestar branch, Islamic Azad University, Shabestar, Iran Author-Name: J. Ghiasi-Ghalehkandi Author-Workplace-Name: Department of Animal Science, Shabestar branch, Islamic Azad University, Shabestar, Iran Author-Name: H. Kaiya Author-Workplace-Name: Department of Biochemistry, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, Japan Author-Name: N. Maheri-Sis Author-Workplace-Name: Department of Animal Science, Shabestar branch, Islamic Azad University, Shabestar, Iran Title: Effect of in ovo ghrelin administration on subsequent serum insulin and glucose levels in newly-hatched chicks Abstract: Ghrelin is a regulatory peptide in glucose homeostasis in animal species. Its effect in the avian embryo is unclear. The aim of this study was to investigate the effects of in ovo ghrelin administration on serum glucose and insulin levels of hatched chicks. A total of 250 fertilized eggs were divided into 5 groups; group T1 as control (without injection), group T2 (in ovo injected with 50 ng/egg ghrelin on day 5), group T3 (in ovo injected with 100 ng/egg ghrelin on day 5), group T4 (in ovo injected with 50 ng/egg ghrelin on day 10) and group T5 (in ovo injected with 100 ng/egg ghrelin on day 10). After hatching, serum insulin and glucose concentrations were determined. Group T4 and T5 showed significantly higher serum insulin levels (0.43 and 0.60 ng/ml, respectively) compared with T1, T2 and T3 (0.09, 0.10, and 0.23 ng/ml, respectively) in hatched chicks. Glucose concentrations have not been affected by in ovo administered ghrelin in all injected groups. It seems that embryonic β-cells were stimulated to secrete a considerable level of insulin in response to in ovo ghrelin in the late embryonic life. The observed stability of glucose rate suggests the incidence of insulin resistance at hatching time or in newly hatched chicks from in ovo ghrelin administered eggs on day 10. Keywords: chicken, embryo, ghrelin, in ovo injection, insulin Journal: Czech Journal of Animal Science Pages: 377-380 Volume: 56 Issue: 8 Year: 2011 DOI: 10.17221/2396-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2396-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201108-0006.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:8:id:2396-CJAS