Template-Type: ReDIF-Article 1.0 Author-Name: W.B. Bao Author-Workplace-Name: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, P.R. China Author-Workplace-Name: Jiangsu Engineering Research Centre for Molecular Breeding of Pig, Changzhou, P.R. China Author-Name: L. Ye Author-Workplace-Name: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, P.R. China Author-Name: Z.Y. Pan Author-Workplace-Name: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, P.R. China Author-Name: J. Zhu Author-Workplace-Name: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, P.R. China Author-Name: G.Q. Zhu Author-Workplace-Name: College of Veterinary Medicine, Yangzhou University, Yangzhou, P.R. China Author-Workplace-Name: Jiangsu Engineering Research Centre for Molecular Breeding of Pig, Changzhou, P.R. China Author-Name: X.G. Huang Author-Workplace-Name: Jiangsu Engineering Research Centre for Molecular Breeding of Pig, Changzhou, P.R. China Author-Name: S.L. Wu Author-Workplace-Name: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, P.R. China Author-Workplace-Name: Jiangsu Engineering Research Centre for Molecular Breeding of Pig, Changzhou, P.R. China Title: Analysis of polymorphism in the porcine TLR4 gene and its expression related to Escherichia coliF18 infection Abstract: The genetic variation in exon 1 of the TLR4 gene was detected among a total of 893 animals, including Asian wild boars, 3 imported commercial and 10 Chinese indigenous pig breeds. The expression of TLR4 was assayed by RT-PCR and different expression between resistant and sensitive resource populations to ETEC F18 was analysed to discuss the role that the TLR4 gene plays in resistance. In this study, new alleles were detected in exon 1 of the TLR4 gene. These polymorphisms are significantly different between Chinese indigenous breeds and imported breeds. Based on the published TLR4 gene sequence (AB232527) in GenBank, a 93G > C mutation was found in 5'UTR and only a 194G > A synonymous mutation was found in the coding sequence of exon 1. In addition, TLR4 gene was broadly expressed in 11 tissues with the highest level in lung. The expression was relatively high in the lymph nodes, kidney and spleen. Generally, the expression of TLR4 gene in sensitive individuals was higher than that in resistant individuals. The results indicated that the downregulation of the mRNA expression of TLR4 gene had reduced the transmembrane signal transduction of LPS and then led to the responsive ability of the host to ETEC F18 in piglets. Keywords: pigs, TLR4 gene, polymorphism, expression profile, ETEC F1 Journal: Czech Journal of Animal Science Pages: 475-482 Volume: 56 Issue: 11 Year: 2011 DOI: 10.17221/3836-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/3836-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201111-0001.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:11:id:3836-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: M. Szczepkowski Author-Workplace-Name: Department of Sturgeon Fish Breeding, Inland Fisheries Institute, Olsztyn, Poland Author-Name: Z. Zakęś Author-Workplace-Name: Department of Sturgeon Fish Breeding, Inland Fisheries Institute, Olsztyn, Poland Author-Name: B. Szczepkowska Author-Workplace-Name: Department of Sturgeon Fish Breeding, Inland Fisheries Institute, Olsztyn, Poland Author-Name: I. Piotrowska Author-Workplace-Name: Department of Sturgeon Fish Breeding, Inland Fisheries Institute, Olsztyn, Poland Title: Effect of size sorting on the survival, growth and cannibalism in pikeperch (Sander lucioperca L.) larvae during intensive culture in RAS Abstract: The aim of the experiment was to determine the impact of sorting on the growth, survival, and cannibalism of pikeperch larvae during intensive culture in recirculation systems. Larvae aged 48 days post-hatch (DPH) were reared in three groups - small specimens (group S - average body weight 40 mg), large specimens (group L - average body weight 76 mg), and unsorted ones (group U - average body weight 55 mg). After three weeks of rearing, there were no statistically significant differences in specific growth rates among the groups. However, increases in biomass were higher in the sorted groups. Survival exceeded 50% in the sorted groups and 39% in group U. Higher cannibalism was noted in group U than in the sorted groups. Significant differences among the sorted groups were observed in cannibalism, which was higher in group L, and in natural and manipulation losses, which were higher in group S (P < 0.05). During the first two weeks of rearing, the lowest cannibalism rates were observed in group S, the difference between groups S and U was statistically significant (P < 0.05). The results of the experiment indicate that the sorting of pikeperch larvae has a positive impact on the survival rate, however, it has no impact on growth during their rearing in the RAS. Keywords: pikeperch, larvae, sorting, RAS, growth, survival, cannibalism Journal: Czech Journal of Animal Science Pages: 483-489 Volume: 56 Issue: 11 Year: 2011 DOI: 10.17221/3837-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/3837-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201111-0002.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:11:id:3837-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: E. Tůmová Author-Workplace-Name: Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: M. Englmaierová Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: Z. Ledvinka Author-Workplace-Name: Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: V. Charvátová Author-Workplace-Name: Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Title: Interaction between housing system and genotype in relation to internal and external egg quality parameters Abstract: The effect of three housing systems (conventional cages, enriched cages and litter) on egg quality parameters was evaluated in two experiments with four brown laying strains ISA Brown, Hisex Brown, Bovans Brown and Moravia BSL. During 40 weeks of lay the total number of 7200 eggs was produced and analysed for egg weight, egg component weight and eggshell quality indicators. In 60 eggs, pore density in the small-end, large-end and equatorial areas was determined. Significant interactions between genotype and housing were found out in egg weight (P ≤ 0.001), yolk and albumen weight (P ≤ 0.001) and yolk colour (P ≤ 0.001). Haugh units were the highest in eggs laid in cages and the lowest in the ISA Brown strain. Eggshell quality indicators were affected more by genotype than by housing. The interaction between genotype and housing was not significant for eggshell thickness but it was significant for eggshell weight and strength. Although eggshell thickness was lower in eggs produced in cages, eggshell strength was higher. A significant negative correlation was found out between pore density and housing system. Results of the study suggest that the ability of a strain to produce eggs of high quality in a particular housing system should be considered, even within brown strains. Keywords: laying hen, conventional cage, enriched cage, litter, genotype, egg physical measurements, pore density Journal: Czech Journal of Animal Science Pages: 490-498 Volume: 56 Issue: 11 Year: 2011 DOI: 10.17221/3838-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/3838-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201111-0003.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:11:id:3838-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: M.E. Trujillo-Ortega Author-Workplace-Name: National Autonomous University of Mexico (UNAM), FMVZ, CEIEPP and FESC, Mexico City and State of Mexico, Mexico Author-Name: D. Mota-Rojas Author-Workplace-Name: Department of Animal Production and Agriculture, Stress Physiology and Farm Animal Welfare, Metropolitan Autonomous University, Campus Xochimilco (UAM-X), Mexico D.F., Mexico Author-Name: O. Juárez Author-Workplace-Name: Department of Animal Production and Agriculture, Stress Physiology and Farm Animal Welfare, Metropolitan Autonomous University, Campus Xochimilco (UAM-X), Mexico D.F., Mexico Author-Name: D. Villanueva-García Author-Workplace-Name: Division of Neonatology, Mexican Children's Hospital "Federico Gomez", Mexico D.F., Mexico Author-Name: P. Roldan-Santiago Author-Workplace-Name: Department of Animal Production and Agriculture, Stress Physiology and Farm Animal Welfare, Metropolitan Autonomous University, Campus Xochimilco (UAM-X), Mexico D.F., Mexico Author-Name: M. Becerril-Herrera Author-Workplace-Name: EIAH- Benemerita Autonomous University of Puebla, Teziutlán, Puebla, Mexico Author-Name: R. Hernández-González Author-Workplace-Name: Department of Experimental Research and Animal Resources, National Institute of Medical Sciences and Nutrition "Salvador Zubirán", Mexico D.F., Mexico Author-Name: P. Mora-Medina Author-Workplace-Name: National Autonomous University of Mexico (UNAM), FMVZ, CEIEPP and FESC, Mexico City and State of Mexico, Mexico Author-Name: M. Alonso-Spilsbury Author-Workplace-Name: Department of Animal Production and Agriculture, Stress Physiology and Farm Animal Welfare, Metropolitan Autonomous University, Campus Xochimilco (UAM-X), Mexico D.F., Mexico Author-Name: A.M. Rosales Author-Workplace-Name: Department of Animal Production and Agriculture, Stress Physiology and Farm Animal Welfare, Metropolitan Autonomous University, Campus Xochimilco (UAM-X), Mexico D.F., Mexico Author-Name: R. Martínez-Rodríguez Author-Workplace-Name: National Autonomous University of Mexico (UNAM), FMVZ, CEIEPP and FESC, Mexico City and State of Mexico, Mexico Author-Name: R. Ramírez-Necoechea Author-Workplace-Name: Department of Animal Production and Agriculture, Stress Physiology and Farm Animal Welfare, Metropolitan Autonomous University, Campus Xochimilco (UAM-X), Mexico D.F., Mexico Title: Porcine neonates failing vitality score: physio-metabolic profile and latency to the first teat contact Abstract: The objective of this study was to compare the metabolic and electrolytic profile as well as the morphological appearance of the umbilical cord and newly born piglets' weight that failed the vitality test scale compared to those who passed. Newborn piglets were divided into three groups according to the vitality with a modified Apgar score at birth: Group 1, failing with a score < 5 (G1: n = 218), Group 2 had a score of 6 to 7 (G2: n = 439) and Group 3 had scores > 8 (G3: n = 464). Results showed significant differences among groups (P < 0.05) in the physio-metabolic pH, PCO2, PO2, Na+, Ca2+, glucose, lactate and bicarbonate values. Regarding weight, temperature and latency to connect the maternal teat, there were also significant differences (P < 0.05) among groups; it took 23.38 min for G3 while neonatal piglets from G1 took 30 min longer (P < 0.05) to make the first teat contact. The neonates from the latter group had a higher percentage (75.68%) of broken umbilical cords, with higher birth weight (+200 g, P < 0.05), showed higher than normal blood glucose concentrations, and had lower body temperature at birth (-0.7°C, P < 0.05) and PO2 in comparison with the other groups of neonates that passed the vitality score. A novel point of this study is the profile characterization of piglets that failed and passed the vitality score; we expect that the data provided may be applicable as reference values of metabolic and electrolyte blood profiles in newborn piglets according to their vitality. In conclusion, this study demonstrates that low vitality newborn piglets had clearly undergone through perinatal asphyxia. Potential indicators increasing this condition are: high birth weight, low body temperature, vitality score ≤ 5, and the presence of the broken umbilical cord at birth. Keywords: piglet, welfare, haemodynamics, acidosis, asphyxia, neonate, Apgar score Journal: Czech Journal of Animal Science Pages: 499-508 Volume: 56 Issue: 11 Year: 2011 DOI: 10.17221/3839-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/3839-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201111-0004.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:11:id:3839-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: J. Ducháček Author-Workplace-Name: Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Animal Husbandry, Prague, Czech Republic Author-Name: J. Přibyl Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: L. Stádník Author-Workplace-Name: Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Animal Husbandry, Prague, Czech Republic Author-Name: L. Vostrý Author-Workplace-Name: Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Animal Husbandry, Prague, Czech Republic Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: J. Beran Author-Workplace-Name: Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Animal Husbandry, Prague, Czech Republic Author-Name: L. Štolc Author-Workplace-Name: Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Animal Husbandry, Prague, Czech Republic Title: Stability of Aberdeen Angus breeding values in the Czech Republic from 1997 to 2007 Abstract: We predict the stability of breeding values (BVs) for direct effect (DE) and maternal effect (ME) for live weights at 210 days of age in the entire population of purebred Aberdeen Angus cattle in the Czech Republic according to an increase of progeny number in performance recording over a period of 11 years (1997 to 2007) and the course of BVs for DE and ME during the years of observation in animals born until 1997. Furthermore we compare genetic trends of BVs for DE and ME among animals born in different years and detect the level and significance of correlation coefficients among predictions of BVs for DE and ME performed during the years of observation. The animal model and the BLUPF90 programme were used for these predictions. The used model included the effects of animal, sex, contemporary group, dam, age of dam, and permanent environment of dam. The variance of BVs ranged from 4.96 to 10.87 depending on the year of evaluation and whether it was related to maternal or direct genetic effect. The animals were initially assigned to groups according to their BV in 1997, and this ranking was not affected by the BVs predicted in subsequent years. The existence of a negative correlation between direct and maternal effects was confirmed. The significant correlations (P < 0.0001) demonstrated a strong relationship between the BVs predicted in successive years, e.g. the correlation coefficient for the relationship between BVs for direct effect predicted in the last years of the examined period was above 0.9 and that for maternal effect was above 0.8. Keywords: Aberdeen Angus, breeding value, BLUP, weaning weight, stability of breeding value, direct effect, maternal effect Journal: Czech Journal of Animal Science Pages: 509-520 Volume: 56 Issue: 11 Year: 2011 DOI: 10.17221/3840-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/3840-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201111-0005.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:11:id:3840-CJAS