Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: Volume 56, Contents Author Index 2011 Journal: Czech Journal of Animal Science Pages: I-V Volume: 56 Issue: 12 Year: 2011 DOI: 10.17221/4418-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/4418-CJAS.html File-Format: text/html Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:12:id:4418-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: S. Sato Author-Workplace-Name: National Livestock Breeding Center, Nishigo, Fukushima, Japan Author-Name: C. Ohnishi Author-Workplace-Name: National Livestock Breeding Center, Nishigo, Fukushima, Japan Author-Name: Y. Uemoto Author-Workplace-Name: National Livestock Breeding Center, Nishigo, Fukushima, Japan Author-Name: E. Kobayashi Author-Workplace-Name: National Livestock Breeding Center, Nishigo, Fukushima, Japan Title: Haplotype analysis within quantitative trait locus affecting intramuscular fat content on porcine chromosome Abstract: Previous results of fine mapping for quantitative trait loci affecting intramuscular fat content identified a 3.0-Mb chromosome interval on porcine chromosome 7, which contains at least 9 genes, based on the pig genome assembly. Therefore, we proposed these nine genes (LOC100154481, LOC100155711, LOC100155276, SPATA7, PTPN21, ZCH14, EML5, TTC8, and FOXN3) as positional candidate genes. The coding exons of the nine genes were characterized, and 45 polymorphisms were detected in F2 Duroc × Meishan population. Within the nine genes, 10 non-synonymous substitutions and 1 insertion were genotyped among three European breeds (Landrace, Large White, and Duroc) and 1 Chinese breed (Meishan). Genotyping data was used to perform the haplotype analysis. Polymorphisms were found in all the studied genes, except ZCH14. We surveyed the frequency of 33 haplotypes that formed non-synonymous substitutions in four breeds. One of them was distributed widely in the Landrace, Large White, and Meishan breeds, but not in Duroc. Each breed had different major haplotypes. Keywords: pig, chromosome 7, intramuscular fat, positional candidate genes, polymorphism Journal: Czech Journal of Animal Science Pages: 521-528 Volume: 56 Issue: 12 Year: 2011 DOI: 10.17221/4414-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/4414-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201112-0002.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:12:id:4414-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: X.P. An Author-Name: J.G. Wang Author-Name: J.X. Hou Author-Name: H.B. Zhao Author-Workplace-Name: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: L. Bai Author-Workplace-Name: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: G. Li Author-Workplace-Name: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: L.X. Wang Author-Workplace-Name: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: X.Q. Liu Author-Workplace-Name: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: W.P. Xiao Author-Workplace-Name: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: Y.X. Song Author-Workplace-Name: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China Author-Name: B.Y. Cao Author-Workplace-Name: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China Title: Polymorphism identification in the goat MSTN gene and association analysis with growth traits Abstract: The myostatin (MSTN) gene was studied as a candidate genetic marker for growth traits. We investigated polymorphisms of the MSTN gene in 664 individuals from four goat populations and applied PCR-SSCP and DNA sequencing analysis to reveal two single nucleotide polymorphisms (DQ167575: g.368A>C (p.Lys49Thr) and g.4911C>T. At g.368A>Clocus, the frequencies of g.368A allele were 0.75-0.81, and the frequencies of g.368C allele were 0.19-0.25. At g.4911C>T locus, the frequencies of g.4911C allele were 0.76-0.82, and frequencies of g.4911T allele were 0.18-0.24. Compared to the female goats with AC genotype, those with AA genotype had superior body weight in Boer goats (15.69 ± 0.28 vs. 14.51 ± 0.31, P < 0.05) and F1 generation of Boer × Guanzhong dairy goats (19.39 ± 0.34 vs. 18.27 ± 0.33, P < 0.05). In addition, the female goats with AA genotype (45.80 ± 0.33 cm) had greater withers height than those with AC genotype (44.78 ± 0.36 cm) in F2 generation of Boer × Guanzhong dairy goats (P < 0.05). Hence, the biochemical and physiological functions along with the results obtained in our investigation suggest that the MSTN gene might play an important role in affecting the growth traits in goats. Keywords: MSTN gene, polymorphisms, growth traits, linkage disequilibrium Journal: Czech Journal of Animal Science Pages: 529-535 Volume: 56 Issue: 12 Year: 2011 DOI: 10.17221/4415-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/4415-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201112-0003.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:12:id:4415-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: M. Englmaierová Author-Workplace-Name: Department of Nutrition Physiology and Animal Product Quality, Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: I. Bubancová Author-Workplace-Name: Department of Nutrition Physiology and Animal Product Quality, Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: T. Vít Author-Workplace-Name: Department of Nutrition Physiology and Animal Product Quality, Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: M. Skřivan Author-Workplace-Name: Department of Nutrition Physiology and Animal Product Quality, Institute of Animal Science, Prague-Uhříněves, Czech Republic Title: The effect of lycopene and vitamin E on the growth performance, quality and oxidative stability of chicken leg meat Abstract: A 2 × 3 factorial design experiment was conducted to evaluate the effect of adding lycopene (0 and 75 mg/kg) and vitamin E (0.50 and 100 mg/kg) to the diet of chickens. Moreover, the study investigated growth traits, oxidative stability and chemical composition of leg meat and the vitamin content of meat and liver. The study was conducted using five hundred and forty Ross 308 male broilers that were assigned to one of the six dietary treatments. Significant interactions between lycopene and vitamin E additions affected the body weight of 21-days-old chickens (P = 0.005), the malondialdehyde content in fresh leg meat (P < 0.001) and leg meat stored for 3 days at temperatures of 2.5 to 4°C (P = 0.032), the cholesterol content in leg meat (P < 0.001) and the lycopene content in liver (P = 0.006). The chickens with the highest body weight were fed 75 mg/kg of lycopene and 50 mg/kg of vitamin E. The vitamin E supplement increased the oxidative stability of fresh and stored leg muscle (P < 0.001). The lowest mean cholesterol value (3.49 g/kg of dry matter) was found out in the meat from broilers that were fed 75 mg/kg of lycopene in contrast to broilers fed the control treatment without lycopene (3.93 g/kg of dry matter). Dietary vitamin E significantly reduced the fat content (P = 0.033) and increased the ash content of leg meat. The highest lycopene concentration in liver (2.82 mg/kg of dry matter) was in chickens that were fed the highest levels of vitamin E and lycopene in contrast with the control group (0.28 mg/kg of dry matter). Keywords: antioxidants, lycopene, α -tocopherol, broiler, growth traits, meat quality, malondialdehyde Journal: Czech Journal of Animal Science Pages: 536-544 Volume: 56 Issue: 12 Year: 2011 DOI: 10.17221/4416-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/4416-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201112-0004.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:12:id:4416-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Y.C. Zheng Author-Workplace-Name: College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan, P.R. China Author-Name: Y.Q. Lin Author-Workplace-Name: College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan, P.R. China Author-Name: Y. Yue Author-Workplace-Name: Science and Technology Bureau of Ganzi Prefecture, Kangding, Sichuan, P.R. China Author-Name: Y.O. Xu Author-Workplace-Name: College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan, P.R. China Author-Name: S.Y. Jin Author-Workplace-Name: College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan, P.R. China Title: Expression profiles of myostatin and calpastatin genes and analysis of shear force and intramuscular fat content of yak longissimus muscle Abstract: The main objective of this study was to reveal the expression profiles of two negative regulators, myostatin (MSTN) and calpastatin (CAST)genes, of skeletal muscle growth in highland yaks (Bos grunniens). mRNA levels of both genes were quantified in different yak tissues by semi-quantitative RT-PCR to reveal the tissue expression pattern, and real-time quantitative RT-PCR was employed to compare the mRNA levels of MSTN and CAST in longissimus muscles of yaks at different ages and adult Yellow cattle. Intramuscular fat (IMF) content, tenderness and pH of longissimus muscle of yaks at different ages and of adult Yellow cattle were also measured. The results showed that MSTN and CAST expressions have tissue specificity and both exhibited a high level in longissimus muscle and a low level in adipose tissue. Yak calves had lower mRNA levels of both MSTN and CAST in longissimus muscle compared with adult yaks. The analysis of meat quality traits of longissimus muscle showed that the shear forces of raw longissimus muscle of yak calves were significantly lower than those of adult yaks and Yellow cattle, no significant difference was found between adult yaks and Yellow cattle of similar age. IMF content in longissimus muscle was lower in yaks than in Yellow cattle. Although yaks were smaller in body size than Yellow cattle, adult yaks showed lower levels of MSTN and similar level of CAST mRNA in longissimus muscle compared to Yellow cattle. These data indicate that the expression of both MSTN and CAST in longissimus muscle differs between adult yaks and yak calves, and the yak longissimus muscle shows a lower IMF content compared to cattle. Keywords: Bos grunniens, meat trait, muscle growth, hypoxia, intramuscular fat Journal: Czech Journal of Animal Science Pages: 545-550 Volume: 56 Issue: 12 Year: 2011 DOI: 10.17221/4417-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/4417-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201112-0005.txt Handle: RePEc:caa:jnlcjs:v:56:y:2011:i:12:id:4417-CJAS