Template-Type: ReDIF-Article 1.0 Author-Name: M. Englmaierová Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: G. Dlouhá Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: M. Marounek Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: M. Skřivan Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Title: Efficacy of contrast levels of non-phytate phosphorus and Aspergillus niger phytase in hens fed wheat-maize-based diets Abstract: A 2 × 2 factorial design experiment examined the effect of dietary non-phytate phosphorus (NPP) (1.3 and 4.0 g/kg) and 3-phytase (F) (0 and 150 FTU/kg) on the performance indicators of hens, physical parameters of eggs, phosphorus (P) content of the eggshells, and the pH of the digestive tract of laying hens. Two hundred and forty hens (ISA Brown) were housed in enriched cages and fed a wheat-maize-based diet. A significant effect of both NPP and F was found for the yolk colour (P = 0.016) and shell thickness (P = 0.038). The F supplement or higher level of NPP alone and in combination increased the value of the yolk colour and shell thickness. The supplementation of the basal diet with F significantly increased the laying performance, especially with regard to the egg weight and feed conversion ratio. The higher dose of NPP had a negative effect on the egg production, feed intake, and feed conversion ratio. The lower level of NPP (1.3 g/kg) decreased the internal egg quality and shell quality, except for the shell strength. In contrast, the addition of 3-phytase to the diet increased the shell thickness and shell weight. The P content in the eggshells was not influenced by the dietary treatment. The higher level of NPP or F increased the pH in the gizzard to a value suitable for F activity. The hens fed a diet containing 1.3 g/kg NPP achieved a higher performance; moreover, the F supplement at 150 FTU/kg increased the external quality of the eggs. Keywords: laying hen, egg production, feed intake, eggshell quality, pH Journal: Czech Journal of Animal Science Pages: 499-505 Volume: 57 Issue: 11 Year: 2012 DOI: 10.17221/6382-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/6382-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201211-0001.txt Handle: RePEc:caa:jnlcjs:v:57:y:2012:i:11:id:6382-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: J. Yang Author-Workplace-Name: Key Laboratory of Animal Disease Resistance Nutrition of the Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Sichuan Yaan, P.R.China Author-Name: F. Bai Author-Workplace-Name: Key Laboratory of Animal Disease Resistance Nutrition of the Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Sichuan Yaan, P.R.China Author-Workplace-Name: Test Center for Feed Quality Supervision and Inspection (Chengdu) of the Ministry of Agriculture, Sichuan Chengdu, P.R.China Author-Name: K. Zhang Author-Workplace-Name: Key Laboratory of Animal Disease Resistance Nutrition of the Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Sichuan Yaan, P.R.China Author-Name: X. Lv Author-Workplace-Name: Feed Safety Reference Laboratory of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R.China Author-Name: S. Bai Author-Workplace-Name: Key Laboratory of Animal Disease Resistance Nutrition of the Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Sichuan Yaan, P.R.China Author-Name: L. Zhao Author-Workplace-Name: Test Center for Feed Quality Supervision and Inspection (Chengdu) of the Ministry of Agriculture, Sichuan Chengdu, P.R.China Author-Name: X. Peng Author-Workplace-Name: Key Laboratory of Animal Disease Resistance Nutrition of the Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Sichuan Yaan, P.R.China Author-Name: X. Ding Author-Workplace-Name: Key Laboratory of Animal Disease Resistance Nutrition of the Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Sichuan Yaan, P.R.China Author-Name: Y. Li Author-Workplace-Name: Test Center for Feed Quality Supervision and Inspection (Chengdu) of the Ministry of Agriculture, Sichuan Chengdu, P.R.China Author-Name: J. Zhang Author-Workplace-Name: Test Center for Feed Quality Supervision and Inspection (Chengdu) of the Ministry of Agriculture, Sichuan Chengdu, P.R.China Title: Effects of feeding corn naturally contaminated with AFB1 and AFB2 on performance and aflatoxin residues in broilers Abstract: AFB1 is the most abundant aflatoxin in food and animal feed, generally occurring along with low levels of other aflatoxins. In this experiment, broilers were administered corn that was naturally contaminated with AFB1 and AFB2. We found that the broilers were more sensitive during the starter period and had a poor performance during the grower period. In addition, the broilers showed a significant reduction in feed intake when consuming material contaminated with 134.0 and 23.6 µg/kg of AFB1 and AFB2, respectively. Our results also demonstrate that villus height and the ratio of villus height to crypt depth significantly decreased when the broilers were fed daily with diets contaminated with AFB1 and AFB2. Furthermore, AFB1 residues in livers and breast muscles were determined by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) at levels of 0.137 and 0.016 µg/kg, respectively. The AFM1 residue was also detected in livers at a level of 0.051 µg/kg but it was not detectable in breast muscles. Keywords: mycotoxin, influence, poultry, HPLC-MS/MS Journal: Czech Journal of Animal Science Pages: 506-515 Volume: 57 Issue: 11 Year: 2012 DOI: 10.17221/6383-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/6383-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201211-0002.txt Handle: RePEc:caa:jnlcjs:v:57:y:2012:i:11:id:6383-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: S. Keskin Author-Workplace-Name: Department of Biostatistics, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey Author-Name: A. Kor Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, Yuzuncu Yil University, Van, Turkey Author-Name: S. Karaca Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, Yuzuncu Yil University, Van, Turkey Title: Evaluation of sensory characteristics of sheep and goat meat by Procrustes Analysis Abstract: Relationships between sensory variables, species, feeding systems, and panelists were examined by Procrustes Analysis. Six meat groups considering species and feeding type combinations were evaluated by ten panelists for five sensory characteristics (colour, texture, odour, taste, and acceptability). These characteristics were assessed using a nine-point hedonic scale (9 = extremely enjoy, 1 = extremely dislike). Generalized Procrustes Analysis was performed and 61.11% of the total variability was explained by the first two dimensions that correspond to Factor 1 and Factor 2. The first one accounted for 33.99% and the second for 27.12%. Results showed that pasture conditions were reflected in all sensory characteristics of meat of Hair goat as well as Karakas sheep. This study concluded that meat of sheep and goats reared in pasture conditions was more preferable as concerns sensory characteristics than that of fattening animals or those reared in intensive conditions. Keywords: configuration, consensus, dimension, fattening, goat, pasture, rotation, sheep Journal: Czech Journal of Animal Science Pages: 516-521 Volume: 57 Issue: 11 Year: 2012 DOI: 10.17221/6384-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/6384-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201211-0003.txt Handle: RePEc:caa:jnlcjs:v:57:y:2012:i:11:id:6384-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: J. Bauer Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Workplace-Name: Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: M. Milerski Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: J. Přibyl Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: L. Vostrý Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Workplace-Name: Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Title: Estimation of genetic parameters and evaluation of test-day milk production in sheep Abstract: Genetic parameters and breeding values were estimated based on 11 708 daily milk yields from 2255 lactations (1351 sheep, 19 different flocks) between the years 2004 and 2010. The pedigree covered 2334 individuals, including both the recorded animals and their known ancestors. The fixed effects were estimated by the least-squares method, while the genetic parameters were estimated by the REML method. The data were tested by 49 models, but on the basis of the coefficient-of-determination value and the significance of the effects, only 10 models were used for REML analysis. The most suitable model was chosen on the basis of the breeding values distribution and the heritability of daily milk production, which was estimated at 0.28. The fixed effects of the flock parity number, the flock test day, and the linear and quadratic coefficients of flock's days-in-milk in the chosen model were all highly significant (P < 0.0001) for the test-day milk yield. The breeding values had a normal distribution and a standard deviation of 0.46 kg. Keywords: dairy, heritability, model selection, Lacaune, East Friesian, breeding values Journal: Czech Journal of Animal Science Pages: 522-528 Volume: 57 Issue: 11 Year: 2012 DOI: 10.17221/6385-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/6385-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201211-0004.txt Handle: RePEc:caa:jnlcjs:v:57:y:2012:i:11:id:6385-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: K. Saelim Author-Workplace-Name: Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Thailand Author-Name: N. Sohsomboon Author-Workplace-Name: Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Thailand Author-Name: S. Kaewsuwan Author-Workplace-Name: Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Thailand Author-Name: S. Maneerat Author-Workplace-Name: Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Thailand Title: Probiotic properties of Enterococcus faecium CE5-1 producing a bacteriocin-like substance and its antagonistic effect against antibiotic-resistant enterococci in vitro Abstract: A bacteriocin-like substance (BLS) producing Enterococcus faecium CE5-1 was isolated from the gastrointestinal tract (GIT) of Thai indigenous chickens. Investigations of its probiotic potential were carried out. The competition between the BLS probiotic strain and antibiotic-resistant enterococci was also studied. Ent. faecium CE5-1 exhibited a good tolerance to pH 3.0 after 2 h and in 7% fresh chicken bile after 6 h, but the viability of Ent. faecium CE5-1 decreased by about 2-3 log CFU/ml after 2 h incubation in pH 2.5. It was susceptible to the antibiotics tested (tetracycline, erythromycin, penicillin G, and vancomycin). The maximum BLS production from Ent. faecium CE5-1 was observed at 15 h of cultivation. It showed activity against Listeria monocytogenes DMST17303, Pediococcus pentosaceus 3CE27, Lactobacillus sakei subsp. sakei JCM1157, and antibiotic-resistant enterococci. The detection by polymerase chain reaction (PCR) in the enterocin structural gene determined the presence of enterocin A gene in Ent. faecium CE5-1 only. Ent. faecium CE5-1 showed the highest inhibitory activity against two antibiotic-resistant Ent. faecalis VanB (from 6.68 to 4.29 log CFU/ml) and Ent. gallinarum VanC (from 6.76 to 4.31 log CFU/ml) after 12 h of co-cultivation. The results show the future possible use of Ent. faecium CE5-1 as a probiotic strain for livestock to control antibiotic-resistant enterococci. Keywords: chicken, gastrointestinal tract, bile salt, acid tolerance, Enterococcus Journal: Czech Journal of Animal Science Pages: 529-539 Volume: 57 Issue: 11 Year: 2012 DOI: 10.17221/6386-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/6386-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201211-0005.txt Handle: RePEc:caa:jnlcjs:v:57:y:2012:i:11:id:6386-CJAS