Template-Type: ReDIF-Article 1.0 Author-Name: Lei Zhang Author-Workplace-Name: College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China Author-Name: Xiao-Rui Liu Author-Workplace-Name: College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China Author-Name: Peng Han Author-Workplace-Name: College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China Author-Name: Zhan-Qin Zhou Author-Workplace-Name: College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China Author-Name: Bin-Yun Cao Author-Name: Yu-Xuan Song Author-Workplace-Name: College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China Title: The study of endometrium at gestational days 5 and 15 in dairy goats (Capra hircus) Abstract: Endometrium receptivity, an absolutely necessary part of successful embryo implantation, and several morphological and biochemical endometrial receptivity biomarkers have already been studied and proposed in some animals. However, to our knowledge, no such a study has as yet been undertaken in dairy goat. In the present study, the serum and uterus of dairy goat were studied at gestational days 5 and 15, the estrogen (E2) and progesterone (P4) concentrations in serum were determined by electrochemiluminescence immunoassay, the surface topography of endometrium was observed by scanning electron microscopy. Furthermore, the estrogen receptors (ER), prolactin (PRL), some marker genes of receptive endometrium, and cell proliferation and apoptosis in the uterus were also detected. Well-formed pinopodes were found on the surface of endometrium at day 15 with higher E2 and P4 concentrations in the serum and higher estrogen receptors ERα and ERβ expression levels and a lower PRL level in the endometrium. Moreover, some expression levels of marker genes of receptive endometrium (OPN, VEGF, LIF, PRLR) were increased at day 15 compared to day 5, but no significant differences were observed in the cell proliferation and apoptosis in the uterus. The results showed that the endometrium reached the receptive state at gestational day 15 in dairy goats. Keywords: endometrial receptivity, pinopodes, gestational day 15 (D15) Journal: Czech Journal of Animal Science Pages: 358-367 Volume: 62 Issue: 9 Year: 2017 DOI: 10.17221/15/2017-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/15/2017-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201709-0001.txt Handle: RePEc:caa:jnlcjs:v:62:y:2017:i:9:id:15-2017-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Martina Geigerová Author-Workplace-Name: Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Roman Švejstil Author-Workplace-Name: Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Eva Skřivanová Author-Workplace-Name: Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Eva Straková Author-Workplace-Name: Department of Animal Nutrition, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Science Brno, Brno, Czech Republic Author-Name: Pavel Suchý Author-Workplace-Name: Department of Animal Husbandry and Animal Hygiene, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Science Brno, Brno, Czech Republic Title: Effect of dietary lupin (Lupinus albus) on the gastrointestinal microbiota composition in broiler chickens and ducks Abstract: The purpose of the study was to evaluate the amount of raffinose-series oligosaccharides (RSO) in soybean meal (SBM), whole white lupin seed meal (WLM), sunflower meal (SFM), and rapeseed oil meal (ROM) and to determine whether partial or complete dietary WLM replacement affected the numbers of bacteria in selected groups in the microbiota of broiler chickens and ducks without inducing any weight loss. Total counts of anaerobes, lactobacilli, bifidobacteria, and Escherichia coli in caecal samples from both ducks and broiler chickens, as well as in a crop chyme, in broiler chickens, were determined. Live weights before slaughter were determined. Both broiler chickens and ducks were fed a control diet with SBM (L0) or diet containing 50% or 100% WLM as a substitute for SBM (groups L50 and L100, respectively). In comparison with SBM, WLM contained significantly higher amounts of RSO, and the amounts of oligosaccharides in SFM (1.73 ± 0.26 g/100 g) and ROM (1.79 ± 0.14 g/100 g) were negligible compared to those in WLM (8.26 ± 0.14 g/100 g) and SBM (6.96 ± 0.21 g/100 g). The inclusion of lupin in chicken diets did not significantly affect the monitored bacterial groups in crop chyme, but a complete replacement of SBM with WLM (L100 group) in chicken diets significantly (P ≤ 0.05) increased the counts of lactobacilli in caecal samples. Partial (L50 group) and complete (L100 group) lupin supplementation in the duck diet significantly (P ≤ 0.05) increased counts of lactobacilli and bifidobacteria by at least one order of magnitude. E. coli counts in poultry were not affected by changes in diet. The results of our study indicate that partial dietary replacement of SBM with WLM did not significantly affect the live weight of broiler chickens and ducks, but that complete replacement of SBM with WLM may lead to weight loss. Keywords: bacterium, poultry, prebiotic, white lupin, raffinose-series oligosaccharides Journal: Czech Journal of Animal Science Pages: 369-376 Volume: 62 Issue: 9 Year: 2017 DOI: 10.17221/42/2017-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/42/2017-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201709-0002.txt Handle: RePEc:caa:jnlcjs:v:62:y:2017:i:9:id:42-2017-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Eva Tůmová Author-Name: Jana Vlčková Author-Workplace-Name: Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Darina Chodová Author-Workplace-Name: Czech University of Life Sciences Prague, Prague, Czech Republic Title: Differences in oviposition and egg quality of various genotypes of laying hens Abstract: The differences in egg production traits in six laying hen genotypes (brown-egg hens Bovans Brown, Bovans Sperwer, ISA Sussex; white-egg hen Dekalb White; laying hens with tinted shells Moravia Barred and Moravia BSL) and the interactions of genotype and oviposition on egg quality were evaluated. The genotype affected the laying rate (P ≤ 0.003), mean sequence length (P ≤ 0.001), and time of oviposition (P ≤ 0.001). The brown-egg genotypes laid eggs approximately 1 h earlier than the white-egg genotypes and approximately 2 h earlier than the tinted-egg genotypes. Egg shell strength was the lowest in tinted-egg genotypes (P ≤ 0.001) and declined with the time of oviposition (P ≤ 0.002). Egg shell percentage (P ≤ 0.011) and thickness (P ≤ 0.011) were affected by the interaction of genotype and oviposition. None of the effects affected egg weight; however, the proportion of egg components was significantly influenced by the interaction of genotype and oviposition. The study confirmed that the differences in the egg production and egg quality of various genotypes are associated with the laying pattern. Keywords: layers, genotype, sequence, time of collection, egg quality Journal: Czech Journal of Animal Science Pages: 377-383 Volume: 62 Issue: 9 Year: 2017 DOI: 10.17221/22/2017-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/22/2017-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201709-0003.txt Handle: RePEc:caa:jnlcjs:v:62:y:2017:i:9:id:22-2017-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Hossam El-Din Rushdi Author-Workplace-Name: Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt Author-Name: Reda Elwany Abdelhaleem Moghaieb Author-Workplace-Name: Department of Genetics, Faculty of Agriculture, Cairo University, Giza, Egypt Author-Name: Hamdy Abdel-Shafy Author-Workplace-Name: Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt Author-Name: Mohamed Abd El-Aziz Mohamed Ibrahim Author-Workplace-Name: Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt Title: Association between microsatellite markers and milk production traits in Egyptian buffaloes Abstract: The objectives were to evaluate polymorphism in ten microsatellite markers and to demonstrate their association with milk production traits in the Egyptian buffaloes, based on the cross-species transferability of microsatellites from cattle to buffalo. A total of 17 439 daily milk records from the first five lactations were subjected to analyses, in which records from 5 to 290 days in milk were only included. The analysis revealed that eight out of the ten bovine markers analyzed were polymorphic. The means of the number of alleles, effective number of alleles, and fixation index within markers were 4.125, 2.479, and 0.062, respectively. The means of the observed and expected heterozygosity were 0.491 and 0.527 per marker, respectively. The eight polymorphic microsatellites (BM1706, BMS711, BM143, BM415, BM6438, ETH131, BM1443, ETH2) showed significant (P < 0.001) associations with average daily milk yield deviation. Protein percentage was significantly associated with microsatellites BM6438 (P < 0.01) and ETH131 (P < 0.001). Only marker BM415 had a significant (P < 0.05) influence on protein yield. None of the analyzed markers revealed significant effect on fat yield and percentage. The results obtained support future application of the polymorphic microsatellites for detailed studies of the Egyptian buffalo genome. Keywords: relationship, microsatellites, milk yield, milk composition, bubaline genome Journal: Czech Journal of Animal Science Pages: 384-391 Volume: 62 Issue: 9 Year: 2017 DOI: 10.17221/80/2016-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/80/2016-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201709-0004.txt Handle: RePEc:caa:jnlcjs:v:62:y:2017:i:9:id:80-2016-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Lucia Mačuhová Author-Workplace-Name: Research Institute for Animal Production Nitra, National Agricultural and Food Centre, Nitra, Slovak Republic Author-Name: Vladimír Tančin Author-Workplace-Name: Research Institute for Animal Production Nitra, National Agricultural and Food Centre, Nitra, Slovak Republic Author-Workplace-Name: Department of Veterinary Science, Faculty of Agrobiology and Food Resources, Slovak Agricultural University, Nitra, Slovak Republic Author-Name: Juliana Mačuhová Author-Workplace-Name: Institute for Agricultural Engineering and Animal Husbandry, Poing, Germany Author-Name: Michal Uhrinčať Author-Workplace-Name: Research Institute for Animal Production Nitra, National Agricultural and Food Centre, Nitra, Slovak Republic Author-Name: Lucie Hasoňová Author-Workplace-Name: Faculty of Agriculture, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic Author-Name: Jana Margetínová Author-Workplace-Name: Research Institute for Animal Production Nitra, National Agricultural and Food Centre, Nitra, Slovak Republic Title: Effect of ewes entry order into milking parlour on milkability and milk composition Abstract: The aim of the present investigation was to evaluate how the order in which the ewes in a milking group enter the milking parlour affects their milkability and milk composition. Therefore, the order of entry into the milking parlour was evaluated in ewes of one flock (n = 353) during six evening milkings. In all, the sheep were milked in 15 milking groups. The sheep entering the milking parlour in the first milking group achieved 15 points and the sheep of each next milking group one point less, i.e. the sheep of the last group achieved 1 point. In the analysis, only the ewes with the highest and the lowest average number of points were included and assigned to the first (FG, n = 19) and the last group (LG, n = 29), respectively. After the last milking, the individual milk samples were collected from the jar to analyze the composition and somatic cell count. Machine milk yield in 30 s (0.15 ± 0.09 and 0.11 ± 0.05 l) and 60 s (0.26 ± 0.16 and 0.19 ± 0.10 l), peak flow rate (1.04 ± 0.39 and 0.77 ± 0.29 l/min), and latency time (14 ± 3 and 20 ± 13 s) significantly differed (P < 0.05) between FG and LG, respectively. Total milk yield (0.41 ± 0.17 and 0.35 ± 0.14 l) and machine milk yield (0.27 ± 0.15 and 0.22 ± 0.10 l) tended to be higher (P = 0.05 and P = 0.09) in FG than in LG, respectively. No significant differences were observed in milk composition between FG and LG. It seems that ewes which enter the milking parlour in early milking groups have better parameters of milkability than those milked in later groups. Keywords: sheep, milking, milking order, milk flow Journal: Czech Journal of Animal Science Pages: 392-402 Volume: 62 Issue: 9 Year: 2017 DOI: 10.17221/11/2016-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/11/2016-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201709-0005.txt Handle: RePEc:caa:jnlcjs:v:62:y:2017:i:9:id:11-2016-CJAS