Template-Type: ReDIF-Article 1.0 Author-Name: Jindřich Čítek Author-Workplace-Name: Faculty of Agriculture, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic Author-Name: Lenka Hanusová Author-Workplace-Name: Faculty of Agriculture, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic Author-Name: Michaela Brzáková Author-Workplace-Name: Faculty of Agriculture, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic Author-Name: Libor Večerek Author-Workplace-Name: Faculty of Agriculture, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic Author-Name: Lothar Panicke Author-Workplace-Name: Leibniz Institute for Farm Animal Biology, Dummerstorf, Germany Author-Name: Lucie Lískovcová Author-Workplace-Name: Faculty of Agriculture, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic Title: Associations between gene polymorphisms, breeding values, and glucose tolerance test parameters in German Holstein sires Abstract: The association between several gene polymorphisms, the estimated breeding values for milk performance traits, and glucose metabolism measured by the glucose tolerance test (GTT) in German Holstein sires were evaluated. Polymorphisms in DGAT1, GH1, GHR, FASN, and OLR1 genes were not associated with the GTT. A significant relationship was obtained for the DGAT1 AA/GC polymorphism and estimated breeding values for milk performance (milk yield, fat and protein yield, fat and protein percentage). The polymorphism in GHR was significantly associated with estimated breeding values for fat yield, and the polymorphism in OLR1 with estimated breeding value for protein yield. It shows the importance of the polymorphisms and makes their use in the breeding possible. GTT may be helpful in metabolic analyses, but the gene polymorphisms assessed in our study were not associated with GTT traits and further studies should examine other gene polymorphisms to support the role of GTT for potential breeding purposes. Keywords: Bos taurus, milk, glucose metabolism, DGAT1, GH1, GHR, FASN, OLR1, ABCG2 Journal: Czech Journal of Animal Science Pages: 167-173 Volume: 63 Issue: 5 Year: 2018 DOI: 10.17221/8/2017-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/8/2017-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201805-0001.txt Handle: RePEc:caa:jnlcjs:v:63:y:2018:i:5:id:8-2017-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Hu Cui Author-Workplace-Name: Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China Author-Workplace-Name: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China Author-Name: Hao Nie Author-Workplace-Name: Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China Author-Name: Tie-Tao Zhang Author-Workplace-Name: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, P.R. China Author-Name: Zhong-Cheng Wang Author-Workplace-Name: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China Author-Name: Xiuh-Ha Gao Author-Workplace-Name: Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China Author-Workplace-Name: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China Author-Workplace-Name: National Engineering Research Center of Biological Feed, Beijing, P.R. China Author-Name: Fu-He Yang Author-Workplace-Name: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, P.R. China Author-Name: Xiu-Mei Xing Author-Workplace-Name: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, P.R. China Author-Name: Bo Shi Author-Workplace-Name: Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China Author-Workplace-Name: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China Title: Effects of zinc sources and levels on zinc bioavailability, blood parameters, and nutrient balance of male mink (Neovison vison) Abstract: The objective of this study was to investigate the effects of different sources and levels of zinc (Zn) on nutrient digestibility, plasma metabolites, and relative Zn bioavailability in male mink. Animals in the control group were fed a basal diet, consisting mainly of corn, soybean oil, meat and bone meal, and fish meal, with no Zn supplementation. Mink in the other 9 treatments were fed the basal diet supplemented with Zn from grade Zn sulfate (ZnSO4. 7H2O), Zn glycinate (ZnGly), or Zn pectin oligosaccharides (ZnPOS) chelate at concentrations of either 100, 300, or 900 mg Zn/kg dry matter. The results showed that zinc levels increased the AD of fat linearly (P < 0.05). The AD of fat in Zn-900 was higher (P < 0.05) than that of the control. Fecal Zn and urinary Zn were affected by dietary Zn addition (P < 0.01). Moreover, Zn supplementation increased Zn retention compared with the control group (P < 0.05). The N retention in ZnPOS was higher (P < 0.05) than that of the control. The effect of Zn level was linear (P < 0.01) for N retention. In addition, the activity of alkaline phosphatase was higher in groups supplemented with 900 mg/kg Zn (P < 0.05) compared with the control group. There were significant interactions (P < 0.05) among Zn sources on the activity of Cu-Zn superoxide dismutase (Cu-ZnSOD). Compared with ZnSO4, relative bioavailability values were 148% and 173% for ZnGly and ZnPOS, respectively, based on Cu-ZnSOD activity. In conclusion, our data show that the relative bioavailability of ZnPOS was greater than that of ZnSO4. 7H2O and ZnGly and Zn supplementation can enhance the Cu-ZnSOD of male mink, and mink can efficiently utilize ZnGly and ZnPOS. Keywords: zinc pectin oligosaccharides chelate, nutrient digestibility, relative bioavailability Journal: Czech Journal of Animal Science Pages: 174-181 Volume: 63 Issue: 5 Year: 2018 DOI: 10.17221/34/2017-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/34/2017-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201805-0002.txt Handle: RePEc:caa:jnlcjs:v:63:y:2018:i:5:id:34-2017-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Miloš Skřivan Author-Name: Michaela Englmaierová Author-Workplace-Name: Department of Nutrition Physiology and Animal Product Quality, Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: Věra Skřivanová Author-Workplace-Name: Department of Nutrition Physiology and Animal Product Quality, Institute of Animal Science, Prague-Uhříněves, Czech Republic Title: Negative effect of phytase superdosing in laying hens Abstract: Hisex hens, aged 24 weeks, were divided into 6 groups. Each group consisted of 4 cages with 10 hens per cage with dimensions and equipment meeting the EU directives. This 2 × 3 factorial experiment included two levels of calcium (Ca; 35 or 42 g/kg) and 3 different additions of 6-phytase OptiPhos® (Ph; 0, 300, and 1500 phytase units (FTU)/kg) to the diet. The content of nonphytate phosphorus (NPP) in all diets was the same (1.8 g/kg). The experiment lasted 12 weeks. A significant interaction of Ph × Ca (P = 0.029) was observed in hen-day egg production. A diet with 35 g/kg Ca and the highest dose of Ph (1500 FTU/kg) resulted in a lower hen-day egg production (84.1%) than did the other treatments (91.5-88.6%). Phytase superdosing negatively influenced egg mass production (P = 0.001) and the feed conversion ratio (P = 0.018). Neither Ph nor Ca influenced eggshell ash content. Both additions of Ph into mixed feed decreased Haugh units (P < 0.001). A higher content of Ca in the diet increased shell thickness (P = 0.024) and shell breaking strength (P = 0.039), while Ph addition increased shell percentage (P = 0.004) and shell breaking strength (P = 0.009). The results of this experiment demonstrate the unsuitability of Ph superdosing in mixed feed for laying hens. Keywords: Hisex, dietary calcium, OptiPhos® , performance Journal: Czech Journal of Animal Science Pages: 182-187 Volume: 63 Issue: 5 Year: 2018 DOI: 10.17221/113/2017-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/113/2017-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201805-0003.txt Handle: RePEc:caa:jnlcjs:v:63:y:2018:i:5:id:113-2017-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Guo-Li Zhou Author-Workplace-Name: College of Life Science, Liaocheng University, Liaocheng, P.R. China Author-Name: Yang Cao Author-Workplace-Name: Branch of Animal Husbandry, Jilin Academy of Agricultural Sciences, Gongzhuling, P.R. China Author-Name: You-Zhi Xin Author-Workplace-Name: College of Life Science, Liaocheng University, Liaocheng, P.R. China Author-Name: Yu-Fei Song Author-Workplace-Name: College of Life Science, Liaocheng University, Liaocheng, P.R. China Author-Name: Hai-Guo Jin Author-Workplace-Name: Branch of Animal Husbandry, Jilin Academy of Agricultural Sciences, Gongzhuling, P.R. China Title: Alternative polyadenylation and polymorphisms of 3'untranslated regions of bovine BBOX1 gene Abstract: l-Carnitine, a key element in fatty acid metabolism and energy production, is biosynthesized from gamma-butyro-betaine by the catalysis of gamma-butyrobetaine hydroxylase (BBOX1). We cloned three different 3'untranslated regions (3'UTRs) alternative polyadenylation (APA) transcripts of the BBOX1 gene with different 3'UTR length (GenBank Accession Nos. KX431577, KX431578, KX431579). Two polymorphisms, NM_001101881.2: g.1797_1798insTGC and g.1935T>C, were revealed in 3'UTR of BBOX1 gene. They created or disrupted a restriction site for endonuclease BbvI and HincII, respectively. Moreover, the single nucleotide polymorphism (SNP) g.1935T>C can create or disrupt polyadenylation signals PAS3 resulting in the presence of APA3 transcript variant. Marker-trait association analyses showed that the BBOX1-BbvI and BBOX1-HincII loci were significantly associated with muscle fibre diameter, shear force, net meat weight, and carcass weight (P < 0.01). Moreover, we also found a significant association of combined genotypes with cooking loss, muscle fibre diameter, shear force, net meat weight, and carcass weight (P < 0.01). The results of this study provide the evidence that polymorphisms in BBOX1 gene are associated with meat quality and carcass traits in Chinese Red cattle, and may be used as a candidate for marker assisted selection in beef cattle breeding program. Keywords: candidate gene, transcript variants, meat quality, carcass traits, association analysis Journal: Czech Journal of Animal Science Pages: 188-194 Volume: 63 Issue: 5 Year: 2018 DOI: 10.17221/105/2016-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/105/2016-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201805-0004.txt Handle: RePEc:caa:jnlcjs:v:63:y:2018:i:5:id:105-2016-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Marko Halo Author-Name: Eva Mlyneková Author-Workplace-Name: Department of Animal Husbandry, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic Author-Name: Michaela Horná Author-Workplace-Name: Department of Animal Husbandry, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic Author-Name: Monika Ivančíková Author-Workplace-Name: Department of Animal Husbandry, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic Author-Name: Andrea Hrdá Author-Workplace-Name: Department of Animal Husbandry, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic Title: Evaluation of genetic variability of the breed Norik of Muran according to pedigree information Abstract: The Norik of Muran, a unique draught horse bred in Slovakia, belongs to country's biodiversity treasures. The genetic diversity of this horse type was evaluated on the basis of indicators derived from the common ancestry and the probability of gene origin. The pedigree file of the analyzed horses involved 115 individuals (15 stallions and 100 mares). The number of complete generations was 4.49 on average. The maximum number of ancestor generations at the examined population of living horses was 5.38 and the equivalent number in the generation of ancestors was 5.14. The highest average length of the generation interval was 10.97 years in the father-son direction compared to father-daughter (9.74), mother-son (10.87), and mother-daughter (8.99 years - the lowest average length). The generation interval overall average length was 10.14 years. The total coefficient of relatedness was 1.72% on average. The efficient number of core ancestors evenly used in breeding in comparison with the core ancestors mildly decreased to 198. Therefore the Austrian Norik incorporation in the breeding program is the opportunity how to maintain genetic diversity. Keywords: draught horse, interbreeding, local breed, gene origin Journal: Czech Journal of Animal Science Pages: 195-200 Volume: 63 Issue: 5 Year: 2018 DOI: 10.17221/20/2017-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/20/2017-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201805-0005.txt Handle: RePEc:caa:jnlcjs:v:63:y:2018:i:5:id:20-2017-CJAS