Template-Type: ReDIF-Article 1.0 Author-Name: Zoltán Bokor Author-Workplace-Name: Department of Aquaculture, Szent István University, Gödöllő, Hungary Author-Name: Balázs Csorbai Author-Workplace-Name: Department of Aquaculture, Szent István University, Gödöllő, Hungary Author-Name: Levente Várkonyi Author-Workplace-Name: Department of Aquaculture, Szent István University, Gödöllő, Hungary Author-Name: Zsolt Szári Author-Workplace-Name: Balaton Fish Management Non-Profit Ltd, Siófok, Hungary Author-Name: Ferenc Fodor Author-Workplace-Name: Balaton Fish Management Non-Profit Ltd, Siófok, Hungary Author-Name: István Ittzés Author-Workplace-Name: Self-entrepreneur, H-2425 Nagykarácsony, Hungary Author-Name: Daniel Żarski Author-Workplace-Name: Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland Author-Name: Béla Urbányi Author-Workplace-Name: Department of Aquaculture, Szent István University, Gödöllő, Hungary Author-Name: Gergely Bernáth Author-Workplace-Name: Department of Aquaculture, Szent István University, Gödöllő, Hungary Title: Effects of chilled storage and pH of activating solution on different motility parameters in burbot (Lota lota) sperm Abstract: The effects of a simple saline solution prepared using two different pH (4.4 and 8.5) on sperm motility in burbot were investigated. Results were recorded during a 96-hour chilled storage (4°C) in 24-hour intervals. Measurements were focused on the detailed characteristics of motility using 12 parameters obtained from the Computer-assisted Sperm Analysis (CASA). Significantly higher progressive motility (pMOT), distance average path (DAP), distance curved line, distance straight line (DSL), average path velocity (VAP), curvilinear velocity, straight line velocity, and beat cross frequency (BCF) were observed with the activating solution buffered at pH 8.5 in comparison with pH 4.4. Already after 24 h a significant reduction was measured in pMOT (0 h: 49 ± 24%, 24 h: 12 ± 7%). Similar decreasing tendency was recorded only after 72 h in DAP (0 h: 26 ± 4 µm/s, 72 h: 19 ± 9 µm/s), DSL (0 h: 21 ± 5 µm/s, 72 h: 17 ± 8 µm/s), VAP (0 h: 59 ± 9 µm/s, 72 h: 43 ± 21 µm/s), and BCF (0 h: 28 ± 2 Hz, 72 h: 18 ± 10 Hz). The response of different investigated CASA parameters to different treatments varied in our experiments. According to our studies, numerous burbot sperm motility parameters are sensitive to chilled storage and to low pH of the activating solution. Our results could support the effective sperm quality assessment and successful artificial propagation process in burbot. Keywords: short-term storage, activation, Lota lota, sperm analysis, CASA parameters Journal: Czech Journal of Animal Science Pages: 429-434 Volume: 63 Issue: 11 Year: 2018 DOI: 10.17221/122/2017-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/122/2017-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201811-0001.txt Handle: RePEc:caa:jnlcjs:v:63:y:2018:i:11:id:122-2017-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Daniel Polasik Author-Workplace-Name: Department of Genetics and Animal Breeding, West Pomeranian University of Technology Szczecin, Szczecin, Poland Author-Name: Mirosław Tyra Author-Workplace-Name: Department of Animal Genetics and Breeding, National Research Institute of Animal Production, Balice, Poland Author-Name: Magdalena Szyndler-Nędza Author-Workplace-Name: Department of Animal Genetics and Breeding, National Research Institute of Animal Production, Balice, Poland Author-Name: Grzegorz Żak Author-Workplace-Name: Department of Animal Genetics and Breeding, National Research Institute of Animal Production, Balice, Poland Author-Name: Barry Lambert Author-Workplace-Name: Department of Animal Science and Veterinary Technology, Tarleton State University, Stephenville, USA Author-Name: Arkadiusz Terman Author-Workplace-Name: Department of Genetics and Animal Breeding, West Pomeranian University of Technology Szczecin, Szczecin, Poland Title: Association of miR-208b polymorphism with meat quality traits and texture parameters in pigs Abstract: Previous studies have shown that single nucleotide polymorphism (SNP) (rs328743478) located downstream of pre-miR-208b in MYH7 gene is correlated with fibre number composition and drip loss in pigs. Because fibre characteristics are linked to meat quality, the aim of this study was to analyse the relationships between rs328743478 polymorphism and meat quality and texture parameters. The study utilised 578 pigs belonging to Polish Landrace, Polish Large White, Puławska, Pietrain, Duroc, and Hampshire breeds. Association study was performed for the first three breeds separately and for all six breeds joined together. Obtained results showed a significant influence (P ≤ 0.05) of miR-208b genotypes on water holding capacity (WHC) in the whole population and individual breeds and on intramuscular fat content (IMF) and luminosity in the whole population as well as in Landrace and Puławska breeds, respectively. By analysing texture parameters, we found association (P ≤ 0.05 or P ≤ 0.01) between rs328743478 polymorphism and toughness, firmness, and chewiness measured in m. semimembranosus and m. longissimus lumborum. The highest values of these traits in m. semimembranosus were noticed for AA genotype, however in m. longissimus lumborum for GG in the whole population and some individual breeds. It was found that miR-208b genotypes were also associated with cohesiveness (P ≤ 0.01), springiness, and hardness (P ≤ 0.05) but obtained results were not consistent among breeds and the whole population. The obtained results confirm that miR-208b polymorphism is associated with some economically important traits in pigs. Keywords: miRNAs, SNP, performance traits, Sus scrofa domestica Journal: Czech Journal of Animal Science Pages: 435-442 Volume: 63 Issue: 11 Year: 2018 DOI: 10.17221/31/2018-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/31/2018-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201811-0002.txt Handle: RePEc:caa:jnlcjs:v:63:y:2018:i:11:id:31-2018-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Eva Kašná Author-Name: Ludmila Zavadilová Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: Miloslava Štípková Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Title: Genetic evaluation of clinical mastitis traits in Holstein cattle Abstract: The results obtained from different models for predicting breeding values for clinical mastitis (CM) in Holstein cattle were compared. CM was recorded in 30 882 lactations of 12 793 cows in 8 herds from 1996 to 2016. CM was considered either as an all-or-none binary trait (0 - absence of mastitis; 1 - at least one case of CM per lactation) or as the number of cases. CM is recorded in the first 150 days of lactation or throughout the entire course of lactation. Breeding values were predicted with a single-trait repeatability model and with a bivariate animal model, where CM during the 1st lactation and CM during the 2nd and later lactations were considered as two different traits. Estimated heritability ranged from 0.06 for CM as a 0/1 trait during the first 150 days of lactation in the repeatability model to 0.12 for the number of CM cases during the 1st lactation in the bivariate model. Ranking of the sires with 15 or more daughters and of the cows was performed according to their breeding values, and Spearman correlation coefficients were calculated. Rank correlations of breeding values from the repeatability and bivariate models were stronger for the same parts of lactation (150 vs 305 days; 0.88-0.96) and for the repeatability model and the bivariate model for the 2nd and later lactations (0.95-0.98). Trends of average male and female breeding values according to their birth year were used to assess genetic changes in the population. The average breeding values declined slowly for cows born since 2003 but stayed above neutral value, thus indicating permanent genetic deterioration of mastitis resistance. Keywords: udder health, mastitis resistance, genetic parameters, genetic trend Journal: Czech Journal of Animal Science Pages: 443-451 Volume: 63 Issue: 11 Year: 2018 DOI: 10.17221/105/2018-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/105/2018-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201811-0003.txt Handle: RePEc:caa:jnlcjs:v:63:y:2018:i:11:id:105-2018-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Gustavo Paixão Author-Workplace-Name: Animal and Veterinary Research Centre, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal Author-Name: Alexandra Esteves Author-Workplace-Name: Animal and Veterinary Research Centre, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal Author-Name: Rita Payan-Carreira Author-Workplace-Name: Animal and Veterinary Research Centre, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal Author-Name: Nuno Carolino Author-Workplace-Name: National Institute of Agrarian and Veterinarian Research, Research Unit of Biotechnology and Genetic Resources, Vale de Santarém, Portugal Title: Demographic structure and genetic diversity of the endangered Bísaro pig: Evolution and current status Abstract: Bísaro pig, a local endangered swine breed, has gained popularity in the recent years. A complete pedigree was used to evaluate the population structure and investigate the current breed's genetic variability in two reference populations (AlivePop and AliveBpop). Since the breed's foundation, the number of registered animals, producers, and farrowing records has increased. The mean progeny size for sires was 116.17 ± 210.81, and 3.24% of the breeding boars originated 26.54% of all registered births showing a marked unbalanced use of certain sires. The mean calculated equivalent generations was 4.54, and 98.1% of all the animals had known parents, indicating a good degree of pedigree completion and depth. Approximately half of the individuals were inbred, with average inbreeding values of 10.41, 9.02, and 8.58% for the whole and reference populations, respectively. Considering both reference populations, a low effective founder/ancestor ratio was obtained (1.12), showing a well-balanced founder/ancestor contribution, and subsequent genetic transfer. However, the effective founder/founder ratio was particularly low (0.06 and 0.12) compared with other native breeds. The founder genome/effective founder ratios were 0.37 and 0.41 for AlivePop and AliveBpop. Contrarily to the whole population, the random genetic drift weighted significantly more than the drift due to unequal founder contribution in the reference populations. The effective population size based on the increase in coancestry ranged from 62.39 and 54.66, and 16.74 and 24.84 based on the increase of individual inbreeding, for the whole and reference populations. The genetic conservation index steadily grew over the years with a mean value of 6.53 for the whole pedigree. Genetic variability losses and diffuse structuring might have occurred in the Bísaro population. Notwithstanding, the recent genetic variability indicators seem positive, yet supported by an open herdbook policy. These characteristics must be taken into account to define future mating policies and selection programs. Keywords: conservation, genetic variation, inbreeding, pedigree analysis, swine Journal: Czech Journal of Animal Science Pages: 452-461 Volume: 63 Issue: 11 Year: 2018 DOI: 10.17221/113/2018-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/113/2018-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201811-0004.txt Handle: RePEc:caa:jnlcjs:v:63:y:2018:i:11:id:113-2018-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Anna Stachurska Author-Workplace-Name: Department of Horse Breeding and Use, University of Life Sciences in Lublin, Lublin, Poland Author-Name: Antoni Brodacki Author-Workplace-Name: Institute of Biological Basis of Animal Production, University of Life Sciences in Lublin, Lublin, Poland Author-Name: Marta Liss Author-Workplace-Name: Department of Horse Breeding and Use, University of Life Sciences in Lublin, Lublin, Poland Title: Allele frequencies in loci controlling coat colour in Polish Coldblood horses Abstract: The objective of this study was to estimate the frequencies of alleles which produce coat colour in Polish Coldblood horse population, and to verify the hypothesis that coat colour is not considered in its selection. The analysis included 35 928 horses and their parents having been registered in the studbook over a half-century. Allele frequencies in Agouti (A), Extension (E), Dun (D), Roan (Rn), and Grey (G) loci, in parental and offspring generations, were estimated according to test matings and the square root of recessive phenotype frequency. The population structure is in Hardy-Weinberg equilibrium only at E locus and coat colour is regarded by breeders. Black horses are favoured. Higher E locus homozygosity in blacks than in bays makes it easier to obtain black foals. Dun-diluted, roan and grey coat colours are undesirable and the population has come to consist almost uniformly of basic coat colours. These results show the importance of studies on population genetic structure, which despite no formal criteria for breeding for colour, can considerably change through generations. Keywords: generation, population genetic structure, test mating Journal: Czech Journal of Animal Science Pages: 462-472 Volume: 63 Issue: 11 Year: 2018 DOI: 10.17221/107/2018-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/107/2018-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201811-0005.txt Handle: RePEc:caa:jnlcjs:v:63:y:2018:i:11:id:107-2018-CJAS