Template-Type: ReDIF-Article 1.0
Author-Name: Lei Zhang
Author-Name: Fang Peng
Author-Workplace-Name: College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China
Author-Name: Fangbai Yu
Author-Workplace-Name: College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China
Author-Name: Lu Wan
Author-Workplace-Name: College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China
Author-Name: Zhan-Qin Zhou
Title: Expression of ESR1, PRLR, GHR, and IGF1R in mammary glands of Hu sheep with four teats
Abstract: Supernumerary teats are commonly found in sheep. To investigate the expression levels of hormone receptors in supernumerary teats of Hu sheep, mammary tissue samples were collected from two groups of Hu sheep, four- and two-teat sheep, during adolescent (A), pregnancy (P), lactation (L), and non-pregnancy (N). Using hematoxylin and eosin staining, we found that mammary alveoli were denser in the four-teat sheep than in the two-teat sheep during P and L. Immunohistochemistry and Western blotting were used to detect the expression patterns and relative protein expression levels of the estrogen receptor 1 (ESR1), prolactin receptor (PRLR), growth hormone receptor (GHR), and insulin-like growth factor 1 receptor (IGF1R) in Hu sheep mammary glands during the four periods. All four receptors were mainly expressed in mammary epithelial cells and adipose cells. Furthermore, the expression levels of PRLR and GHR in the four-teat sheep were significantly higher than those in the two-teat sheep during P and L. Our data suggest that four-teat sheep have more developed mammary gland tissue compared with two-teat sheep.
Keywords: supernumerary teats, mammary tissue, immunohistochemistry, hormone receptor
Journal: Czech Journal of Animal Science
Pages: 49-58
Volume: 64
Issue: 2
Year: 2019
DOI: 10.17221/3/2018-CJAS
File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/3/2018-CJAS.html
File-Format: text/html
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Handle: RePEc:caa:jnlcjs:v:64:y:2019:i:2:id:3-2018-CJAS

Template-Type: ReDIF-Article 1.0
Author-Name: Paula Domingo
Author-Workplace-Name: Department of Animal Pathology, Obstetric and Reproduction Area, Faculty of Veterinary Medicine, University of Zaragoza, Spain
Author-Name: Maite Olaciregui
Author-Workplace-Name: Department of Animal Pathology, Obstetric and Reproduction Area, Faculty of Veterinary Medicine, University of Zaragoza, Spain
Author-Name: Noelia González
Author-Workplace-Name: Department of Animal Pathology, Obstetric and Reproduction Area, Faculty of Veterinary Medicine, University of Zaragoza, Spain
Author-Name: Ignacio De Blas
Author-Workplace-Name: Department of Animal Pathology, Animal Health Area, Faculty of Veterinary Medicine, University of Zaragoza, Spain
Author-Name: Lydia Gil
Author-Workplace-Name: Department of Animal Pathology, Obstetric and Reproduction Area, Faculty of Veterinary Medicine, University of Zaragoza, Spain
Title: Comparison of different semen extenders and cryoprotectant agents to enhance cryopreservation of rabbit spermatozoa
Abstract: The purpose of this research was to find a suitable protocol to enhance frozen rabbit sperm preservation analysing the role that seminal plasma (SP) plays and the effect of different cryoprotectant agents on sperm quality 0 and 2 h after thawing. Sperm samples were pooled and divided in eight fractions. Four of them were diluted with BotuCrio® (extender A), INRA 96® plus 6% glycerol (extender B), 6% N, N-dimethylformamide (extender C) and 6% N-methyl-2-pyrrolidone (extender D), respectively. The other four fractions were centrifuged and the supernatant was discarded in order to eliminate SP. Each sample was then resuspended with extender A, B, C and D. Samples were cooled progressively, loaded into 0.5 ml freezing straws and frozen with liquid nitrogen vapour. Thawing was performed by placing the straws into a bain-marie at 37°C for 21 s. Straws were dried and sperm samples placed into Eppendorf tubes to be analyzed by ISAS software, vitality test, HOS test and acrosome integrity test. The best motility and velocity parameters were obtained by extender A (P < 0.050) even when the motility parameter was compared with previous studies using other diluents. Additionally, sperm quality decreased over incubation time (P < 0.050) and no differences were found in samples processed with or without SP. This research revealed that BotuCrio® could be used for rabbit sperm cryopreservation and moreover the improvement of the cryopreservation process of rabbit sperm due to the demonstration that SP removing is not required.
Keywords: Botucrio® , cryoprotectant, dimethylformamide, glycerol, N-methyl-2-pyrrolidone, rabbit sperm preservation
Journal: Czech Journal of Animal Science
Pages: 59-66
Volume: 64
Issue: 2
Year: 2019
DOI: 10.17221/53/2018-CJAS
File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/53/2018-CJAS.html
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Handle: RePEc:caa:jnlcjs:v:64:y:2019:i:2:id:53-2018-CJAS

Template-Type: ReDIF-Article 1.0
Author-Name: Lenka Putnová
Author-Workplace-Name: Laboratory of Agrogenomics, Department of Morphology, Physiology and Animal Genetics, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic
Author-Name: Radek Štohl
Author-Workplace-Name: Department of Control and Instrumentation, Faculty of Electrical Engineering and Communication, Brno University of Technology, Brno, Czech Republic
Author-Name: Irena Vrtková
Author-Workplace-Name: Laboratory of Agrogenomics, Department of Morphology, Physiology and Animal Genetics, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic
Title: Using nuclear microsatellite data to trace the gene flow and population structure in Czech horses
Abstract: Based on a data set comprising 2879 animals and 17 nuclear microsatellite DNA markers, we propose the most comprehensive in-depth study mapping the genetic structure and specifying the assignment success rates in horse breeds at the Czech population scale. The STRUCTURE program was used to perform systematic Bayesian clustering via the Markov chain Monte Carlo estimation, enabling us to explain the population stratification and to identify genetic structure patterns within breeds worldwide. In total, 182 different alleles were found over all the populations and markers, with the mean number of 10.7 alleles per locus. The expected heterozygosity ranged from 0.459 (Friesian) to 0.775 (Welsh Part Bred), and the average level reached 0.721. The average observed heterozygosity corresponded to 0.709, with the highest value detected in the Czech Sport Pony (0.775). The largest number of private alleles was found in Equus przewalskii. The population inbreeding coefficient FIS ranged from -0.08 in the Merens to 0.14 in the Belgian Warmblood. The total within-population inbreeding coefficient was estimated to be moderate. As expected, very large genetic differentiation and small gene flow were established between the Friesian and Equus przewalskii (FST = 0.37, Nm = 0.43). Zero FST values indicated no differences between the Czech Warmblood-Slovak Warmblood and the Czech Warmblood-Bavarian Warmblood. A high level of breeding and connectivity was revealed between the Slovak Warmblood-Bavarian Warmblood, Dutch Warmblood-Oldenburg Horse, Bavarian Warmblood-Dutch Warmblood, and Bavarian Warmblood-Oldenburg Horse. The breeds' contribution equalled about 6% of the total genetic variability. The overall proportion of individuals correctly assigned to a population corresponded to 82.4%. The posterior Bayesian approach revealed a hierarchical dynamic genetic structure in four clusters (hot-blooded, warm-blooded, cold-blooded, and pony). While most of the populations were genetically distinct from each other and well-arranged with solid breed structures, some of the entire sets showed signs of admixture and/or fragmentation.
Keywords: admixture, breed stratification, gene migration, genetic variation, horse, individual assignment
Journal: Czech Journal of Animal Science
Pages: 67-77
Volume: 64
Issue: 2
Year: 2019
DOI: 10.17221/2/2018-CJAS
File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/2/2018-CJAS.html
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Handle: RePEc:caa:jnlcjs:v:64:y:2019:i:2:id:2-2018-CJAS

Template-Type: ReDIF-Article 1.0
Author-Name: Sahana Shivaramu
Author-Workplace-Name: South Bohemian Research Center for Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic
Author-Name: Doi Thi Vuong
Author-Workplace-Name: South Bohemian Research Center for Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic
Author-Name: Miloš Havelka
Author-Workplace-Name: Nishiura Station, South Ehime Fisheries Research Center, Ehime University, Ainan, Japan
Author-Name: Hana Šachlová
Author-Workplace-Name: Fischzucht Rhönforelle GmbH & Co. KG, Gersfeld, Germany
Author-Name: Ievgen Lebeda
Author-Workplace-Name: South Bohemian Research Center for Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic
Author-Name: Vojtěch Kašpar
Author-Workplace-Name: South Bohemian Research Center for Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic
Author-Name: Martin Flajšhans
Author-Workplace-Name: South Bohemian Research Center for Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic
Title: Influence of interspecific hybridization on fitness-related traits in Siberian sturgeon and Russian sturgeon
Abstract: Polyploidy in sturgeons makes them highly susceptible to interspecific hybridization, and these interspecific hybrids have been described in nature as well as in captivity. Nevertheless, the fitness-related traits between sturgeon hybrids and pure species have been poorly compared as yet. In the present study, we compared the reproductive parameters such as fertilization rate and hatching rate, growth traits and genetic polymorphism in the artificially produced hybrids of the Siberian sturgeon (Acipenser baerii) and Russian sturgeon (A. gueldenstaedtii) with their purebreds. Fertilization and hatching rates were found to be significantly higher in Siberian sturgeon (♀) × Russian sturgeon (♂) hybrid group compared to purebreds. The highest cumulative survival rate was determined in purebred groups until 151 days post-hatch (dph); however, this trend changed and Russian sturgeon purebred showed the lowest cumulative survival rate (0.21%) by 913 dph. Similarly, the lowest average body weight was recorded in Russian sturgeon purebred group (264 g). In contrast, the highest average body weight was recorded in Russian sturgeon (♀) × Siberian sturgeon (♂) hybrids (435.3 g) and the highest cumulative survival rate was recorded in Siberian sturgeon (♀) × Russian sturgeon (♂) hybrids (12.32%) by 913 dph. No significant differences were found at heterozygosity levels among studied crosses. Our results showed that studied sturgeon hybrids had higher survival and growth if compared with the purebreds under provided hatchery conditions.
Keywords: Acipenseriformes, aquaculture, hybrid, growth traits, heterosis
Journal: Czech Journal of Animal Science
Pages: 78-88
Volume: 64
Issue: 2
Year: 2019
DOI: 10.17221/165/2018-CJAS
File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/165/2018-CJAS.html
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Handle: RePEc:caa:jnlcjs:v:64:y:2019:i:2:id:165-2018-CJAS

Template-Type: ReDIF-Article 1.0
Author-Name: Anna Kubešová
Author-Workplace-Name: Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic
Author-Workplace-Name: CEITEC MENDELU, Mendel University in Brno, Brno, Czech Republic
Author-Name: Kamil Šťastný
Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic
Author-Name: Martin Faldyna
Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic
Author-Name: Zbyšek Sládek
Author-Workplace-Name: Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic
Author-Name: Iva Steinhauserová
Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic
Author-Name: Gabriela Bořilová
Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic
Author-Name: Aleš Knoll
Author-Workplace-Name: Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic
Author-Workplace-Name: CEITEC MENDELU, Mendel University in Brno, Brno, Czech Republic
Title: mRNA Expression of CYP2E1, CYP2A19, CYP1A2, HSD3B, SULT1A1 and SULT2A1 genes in surgically castrated, immunologically castrated, entire male and female pigs and correlation with androstenone, skatole, indole and Improvac-specific antibody levels
Abstract: This study aimed to obtain a comprehensive look at the influence of castration on mRNA expression of the genes CYP2E1, CYP1A2, CYP2A19, HSD3B, SULT2A1 and SULT1A1 and their correlation with boar taint compounds (androstenone, skatole and indole) and Improvac-specific antibodies in a Czech commercial hybrid (Large White × Landrace (sow) × Duroc (boar)). Pigs were divided into groups of entire male pigs (NC), pigs castrated surgically (SC), pigs immunologically castrated and slaughtered 8 weeks (IM8) or 15 weeks (IM15) after the second dose of Improvac, and gilts (GI). Hepatic mRNA expression, measured by quantitative real-time polymerase chain reaction, differed significantly between the control group (entire male pigs) and all groups of interest for CYP2E1, CYP1A2 and CYP2A19. The mRNA level of the HSD3B gene differed significantly between the control group and the IM8, IM15 and GI groups. SULT1A1 gene expression was significantly different between the control group and the SC, IM8 and GI. In the case of SULT2A1, a significant difference was observed only between the control group and IM8 pigs. For all genes and treatment groups described above, expression was increased relative to the control. Significant differences for Improvac-specific antibodies between IM8 and IM15 groups were observed, indicating decrease of antibodies over time. Moreover, negative correlations between androstenone and mRNA levels of CYP2A19, CYP2E1 and SULT1A1 suggest that gene expression is suppressed.
Keywords: boar taint, immunocastration, RT-qPCR
Journal: Czech Journal of Animal Science
Pages: 89-97
Volume: 64
Issue: 2
Year: 2019
DOI: 10.17221/159/2018-CJAS
File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/159/2018-CJAS.html
File-Format: text/html
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Handle: RePEc:caa:jnlcjs:v:64:y:2019:i:2:id:159-2018-CJAS