Template-Type: ReDIF-Article 1.0 Author-Name: Wen-Kui Sun Author-Workplace-Name: Key Laboratory for Bio-Resource and Eco-Environment of Education Ministry, Key Laboratory for Animal Disease Prevention and Food Safety of Sichuan Province, College of Life Science, Sichuan University, Chengdu, P.R. China Author-Workplace-Name: Chengdu Medical College, School of Laboratory Medicine, Chengdu, P.R. China Author-Name: Chi Cheng Author-Workplace-Name: College of Chemistry and Life Science, Chengdu Normal University, Chengdu, P.R. China Author-Name: Rui Liu Author-Workplace-Name: Sichuan Academy of Animal Science, Chengdu, P.R. China Author-Name: Yi-Hui Chen Author-Workplace-Name: Key Laboratory for Bio-Resource and Eco-Environment of Education Ministry, Key Laboratory for Animal Disease Prevention and Food Safety of Sichuan Province, College of Life Science, Sichuan University, Chengdu, P.R. China Author-Name: Kai Zeng Author-Workplace-Name: Sichuan Academy of Animal Science, Chengdu, P.R. China Author-Name: Xiao-Hui Chen Author-Workplace-Name: Sichuan Academy of Animal Science, Chengdu, P.R. China Author-Name: Yi-Ren Gu Author-Workplace-Name: Sichuan Academy of Animal Science, Chengdu, P.R. China Author-Name: Jiang-Ling Li Author-Workplace-Name: Sichuan Academy of Animal Science, Chengdu, P.R. China Author-Name: Xue-Bin Lv Author-Workplace-Name: Sichuan Academy of Animal Science, Chengdu, P.R. China Author-Name: Rong Gao Author-Workplace-Name: Key Laboratory for Bio-Resource and Eco-Environment of Education Ministry, Key Laboratory for Animal Disease Prevention and Food Safety of Sichuan Province, College of Life Science, Sichuan University, Chengdu, P.R. China Title: Expression patterns of GHRL, GHSR, LEP, LEPR, SST and CCK genes in the gastrointestinal tissues of Tibetan and Yorkshire pigs Abstract: The aim was to characterize the expression patterns of several genes in the gastrointestinal tracts of Tibetan pigs (TP) and Yorkshire pigs (YP) and to explore their correlation with digestion and growth difference of the two breeds. The body weights and growth of YP and TP were studied at 6, 12 and 24 weeks of age, and their plasma levels of ghrelin (GHRL), leptin (LEP), somatostatin (SST) and cholecystokinin (CCK) were determined by enzyme linked immunosorbent assay (ELISA). Blood and gastrointestinal sections (stomach, duodenum, jejunum, ileum, caecum and colon) were collected and assayed for mRNA expression of the six genes (GHRL, ghrelin receptor (GHSR), LEP, leptin receptor (LEPR), SST and CCK) by reverse transcription-qPCR (RT-qPCR). TP generally had higher mRNA expressions of GHSR, LEP, LEPR, SST and CCK genes compared to YP, and expressed lower levels of the GHRL gene in most tissues of the digestive tract. In both breeds, plasma levels of the expressed proteins were more closely correlated with the feed intake and growth than with mRNA levels of the target genes. Our data indicate that TP possess special gene expression patterns in the gastrointestinal tract compared to YP, which is consistent with its unique feed intake and adaptation to harsh environment. Keywords: gene transcript, hormone level, growth performance, digestive traits Journal: Czech Journal of Animal Science Pages: 239-247 Volume: 64 Issue: 6 Year: 2019 DOI: 10.17221/40/2018-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/40/2018-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201906-0001.txt Handle: RePEc:caa:jnlcjs:v:64:y:2019:i:6:id:40-2018-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Ivana Drzaic Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, University of Zagreb, Zagreb, Croatia Author-Name: Ino Curik Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, University of Zagreb, Zagreb, Croatia Author-Name: Dinko Novosel Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, University of Zagreb, Zagreb, Croatia Author-Name: Vlatka Cubric-Curik Title: Maternal variability of Croatian Spotted goat (Capra hircus) Abstract: Abstract: This study provides the first characterization of maternal ancestry and mitochondrial DNA (mtDNA) diversity in the Croatian Spotted goat (CSG), the most important autochthonous goat breed in Croatia. CSG (n = 25) were randomly sampled from seven herds and a 660-bp fragment from the mtDNA D-loop region was sequenced. Those sequences were compared with 122 corresponding GenBank sequences from goat populations in Albania, Austria, Egypt, Greece, Italy, Romania and Switzerland. CSG showed a great polymorphism (only three out of 17 haplotypes were shared) with high a haplotype (Hd = 0.967 ± 0.019) and nucleotide diversity (π = 0.01305 ± 0.00068). When compared with Mediterranean and ancient goats, all of the 25 CSG were randomly scattered inside haplogroup A showing the weak phylogeographic structure with within-breed variance accounting for 91.76% of the genetic variation. In addition, population expansion tests (mismatch distribution and Fu's Fs statistic) supported these results suggesting at least one population expansion. Keywords: ancestral maternal diversity, D-loop, Mediterranean goat population, phylogeography, unique haplotypes Journal: Czech Journal of Animal Science Pages: 248-254 Volume: 64 Issue: 6 Year: 2019 DOI: 10.17221/240/2018-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/240/2018-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201906-0002.txt Handle: RePEc:caa:jnlcjs:v:64:y:2019:i:6:id:240-2018-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Łukasz Migdał Author-Name: Sylwia Pałka Author-Workplace-Name: Department of Genetics and Animal Breeding, Faculty of Animal Sciences, University of Agriculture in Krakow, Krakow, Poland Author-Name: Michał Kmiecik Author-Workplace-Name: Department of Genetics and Animal Breeding, Faculty of Animal Sciences, University of Agriculture in Krakow, Krakow, Poland Author-Name: Olga Derewicka Author-Workplace-Name: Department of Genetics and Animal Breeding, Faculty of Animal Sciences, University of Agriculture in Krakow, Krakow, Poland Title: Association of polymorphisms in the GH and GHR genes with growth and carcass traits in rabbits (Oryctolagus cuniculus) Abstract: In rabbits, growth and carcass traits are important for the breeding programme. An increasing number of annotated polymorphisms demands validation of their influence on those traits before they can be implemented in breeding practice. Therefore, the aim of this study was to investigate GH c.-78C>T, GHR c.106G>C polymorphisms in the population of Belgian Giant Grey, Termond White, and a crossbreed between New Zealand White and Belgian Giant Grey (NZW × BGG) rabbits. In total 379 animals were genotyped and association analyses with growth traits and carcass traits were conducted. Our results demonstrated that GH c.-78C>T showed an association with growth weight in Belgian Grey and NZW × BGG rabbits. Meat weight in intermediate and hind parts for GH c.-78C>T statistically differed between Belgian Giant Grey and crossbred rabbits. GHR c.106G>C showed an association with meat weight in the intermediate part and dressing percentage in Termond White. TT/CC haplotype in Belgian Giant Grey had significantly higher meat weight in hind part, while in crossbred rabbits CC/CC haplotype was characterised by the lowest meat weight in intermediate and hind parts. Results from our study confirm that GH c.-78C>T, GHR c.106G>C polymorphisms constitute good molecular markers for growth and carcass traits. Keywords: molecular markers, rabbits growth, GH, GHR, association analysis, SNPs Journal: Czech Journal of Animal Science Pages: 255-264 Volume: 64 Issue: 6 Year: 2019 DOI: 10.17221/27/2019-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/27/2019-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201906-0003.txt Handle: RePEc:caa:jnlcjs:v:64:y:2019:i:6:id:27-2019-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Anna Shaliutina-Kolešová Author-Workplace-Name: CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, P.R. China Author-Workplace-Name: Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic Author-Name: Mo Xian Author-Workplace-Name: CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, P.R. China Author-Name: Rui Nian Author-Workplace-Name: CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, P.R. China Title: Antioxidant defence system in protein fractions of common carp (Cyprinus carpio) seminal plasma Abstract: There are growing evidences that the damage which is caused to the spermatozoa by the reactive oxygen species (ROS) plays a key role in male infertility. The seminal plasma is endowed with many enzymatic and non-enzymatic antioxidants which protect the spermatozoa against oxidative stress. The present study was undertaken to compare the level of superoxide dismutase (SOD), glutathione reductase (GR) and glutathione peroxidase (GPx) in separated protein fractions of whole common carp Cyprinus carpio seminal plasma. Seminal plasma from six male common carp was separated into four fractions. The fractions differed from each other in their total seminal plasma protein content and antioxidant status. The highest value of SOD (2.81 ± 0.11 mU/mg protein) was found in fraction 4, and the lowest (1.83 ± 0.18 mU/mg protein) in fraction 1. There were significant variations (ANOVA; P = 0.05) in GR activity, mainly between fractions 1, 2, and 4. The lowest value of GPx activity (14.9 ± 0.4 mU/mg protein) was found in fraction 2; however, the GPx activity showed low variability among fractions. We conclude that distinct seminal plasma fractions differ in antioxidant capacity. The inclusion of fractions with high levels of SOD, GR, and GPx in sperm storage medium may enhance protection against oxidative stress and promote sperm survival during the in vitro storage. Keywords: fish, seminal plasma fractions, proteins, antioxidant activity Journal: Czech Journal of Animal Science Pages: 265-271 Volume: 64 Issue: 6 Year: 2019 DOI: 10.17221/187/2018-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/187/2018-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201906-0004.txt Handle: RePEc:caa:jnlcjs:v:64:y:2019:i:6:id:187-2018-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Jianping Wang Author-Name: Lin Lin Author-Workplace-Name: Department of Animal Production, Henan University of Science and Technology, Luoyang, P.R. China Author-Name: Qingrong Jiang Author-Workplace-Name: Department of Animal Production, Henan University of Science and Technology, Luoyang, P.R. China Author-Name: Wanli Huang Author-Workplace-Name: Department of Animal Production, Henan University of Science and Technology, Luoyang, P.R. China Author-Name: Ning Liu Author-Workplace-Name: Department of Animal Production, Henan University of Science and Technology, Luoyang, P.R. China Title: Effect of supplemental lactic acid bacteria on growth performance, glutathione turnover and aflatoxin B1 removal in lambs Abstract: This study investigated the effect of lactic acid bacteria (LAB) on growth performance, glutathione turnover, aflatoxin B1 (AFB1) residue and AFB1-DNA adduct in growing lambs. Diets were a 2 × 2 factorial design, including AFB1 at 0 or 100 µg/kg and LAB at 0 or 3 × 109 cfu/kg. Results showed that AFB1 decreased (P < 0.05) feed intake (FI), body weight gain (BWG), and activities of glutathione (GSH), glutathione s-transferases (GSTs) and glutathione reductase (GR) in liver and duodenal mucosa, whereas these parameters were increased (P < 0.05) by supplemental LAB. AFB1 increased and LAB decreased the feed conversion ratio (P < 0.05). Interactions (P < 0.05) were found on BWG, liver GSTs and mucosal GSH. LAB decreased (P ≤ 0.001) AFB1 residue in liver, kidney, plasma and faeces and AFB1-DNA adduct in kidney, plasma and faeces. It can be concluded that LAB can partially protect against AFB1 toxicity by facilitating glutathione turnover and reducing AFB1 toxicity in lambs. Keywords: aflatoxin B1-DNA adduct, aflatoxin B1 residue, body weight gain, feed conversion ratio, feed intake Journal: Czech Journal of Animal Science Pages: 272-278 Volume: 64 Issue: 6 Year: 2019 DOI: 10.17221/5/2019-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/5/2019-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201906-0005.txt Handle: RePEc:caa:jnlcjs:v:64:y:2019:i:6:id:5-2019-CJAS