Template-Type: ReDIF-Article 1.0 Author-Name: Szandra Tóth Author-Workplace-Name: Faculty of Agricultural and Environmental Sciences, Kaposvár University, Kaposvár, Hungary Author-Name: Melinda Kovács Author-Workplace-Name: Faculty of Agricultural and Environmental Sciences, Kaposvár University, Kaposvár, Hungary Author-Workplace-Name: Mycotoxins in the Food Chain Research Group, Kaposvár University, Hungarian Academy of Sciences, Kaposvár, Hungary Author-Name: Brigitta Bóta Author-Workplace-Name: Mycotoxins in the Food Chain Research Group, Kaposvár University, Hungarian Academy of Sciences, Kaposvár, Hungary Author-Name: Judit Szabó-Fodor Author-Workplace-Name: Mycotoxins in the Food Chain Research Group, Kaposvár University, Hungarian Academy of Sciences, Kaposvár, Hungary Author-Name: Gábor Bakos Author-Workplace-Name: BOS-FRUCHT Agricultural Production, Processing and Sales Co-operative, Kazsok, Hungary Author-Name: Hedvig Fébel Author-Workplace-Name: Research Institute for Animal Breeding, Nutrition and Meat Science, National Agricultural Research and Innovation Centre, Herceghalom, Hungary Title: Effect of the composition of starter diet fed in the rearing phase on the performance and certain physiological parameters of Holstein calves Abstract: The objective of this study was to compare the effects of calf starters of different starch and fibre content on the growth, blood and rumen parameters of Holstein female calves during the pre-weaning period. A total of 60 calves were divided into two groups. Group A/B was fed according to a two-phase calf nutrition system: the calves received calf starter "A" (28.5% starch content, 16% neutral detergent fibre (NDF), 9.2% acid detergent fibre (ADF)) in days 7-45 and then calf starter "B" (14.2% starch content, 32.4% NDF, 14.3% ADF) in days 46-70. Calves of group B received calf starter "B" throughout the experimental period. The calves were weaned between 52 and 61 days of life. The data on body weight, weight gain, starter feed intake, blood metabolites and rumen fermentation parameters were collected individually. In the first phase of experiment, the starter feed consumption of A/B group was higher than that of B group (P < 0.05), but it did not manifest in differences in body weight at weaning time. At some of the sampling times, the different composition of starter diets significantly (P < 0.05) influenced the plasma urea, albumin, glucose and triglyceride concentrations. The consumption of diet with different starch and NDF content had no significant effect either on the composition of the rumen microbiota or on the ruminal concentration of volatile fatty acids (VFA) and ammonia. In some cases significant correlations (-0.83 and -0.93; 0.82 and 0.90) were found between the composition of rumen microbiota and ruminal VFA concentration. Feeding a diet of higher starch content (28.5 vs 14.2%) in the first 45 days of life did not affect rumen fermentation, rumen microbiota and the most important blood metabolites, apart from a few exceptions. The production parameters of calves, including total feed intake and body weight gain, were similar in the experiment. Keywords: two-phase feeding, dairy calf, rumen fermentation, blood metabolites Journal: Czech Journal of Animal Science Pages: 367-376 Volume: 64 Issue: 9 Year: 2019 DOI: 10.17221/34/2019-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/34/2019-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201909-0001.txt Handle: RePEc:caa:jnlcjs:v:64:y:2019:i:9:id:34-2019-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Alessandra Alves Silva Author-Workplace-Name: Department of Animal Science, Federal University of Viçosa, Viçosa, Brazil Author-Name: Fabyano Fonseca Silva Author-Workplace-Name: Department of Animal Science, Federal University of Viçosa, Viçosa, Brazil Author-Name: Delvan Alves Silva Author-Workplace-Name: Department of Animal Science, Federal University of Viçosa, Viçosa, Brazil Author-Name: Hugo Teixeira Silva Author-Workplace-Name: Department of Animal Science, Federal University of Viçosa, Viçosa, Brazil Author-Name: Cláudio Napolis Costa Author-Workplace-Name: Embrapa Dairy Cattle, Juiz de Fora, Brazil Author-Name: Paulo Sávio Lopes Author-Workplace-Name: Department of Animal Science, Federal University of Viçosa, Viçosa, Brazil Author-Name: Renata Veroneze Author-Workplace-Name: Department of Animal Science, Federal University of Viçosa, Viçosa, Brazil Author-Name: Gertrude Thompson Author-Workplace-Name: Research Center in Biodiversity and Genetic Resources (CIBIO-InBio), University of Porto, Porto, Portugal Author-Workplace-Name: Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal Author-Name: Julio Carvalheira Author-Workplace-Name: Research Center in Biodiversity and Genetic Resources (CIBIO-InBio), University of Porto, Porto, Portugal Author-Workplace-Name: Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal Title: Genotype imputation strategies for Portuguese Holstein cattle using different SNP panels Abstract: Although several studies have investigated the factors affecting imputation accuracy, most of these studies involved a large number of genotyped animals. Thus, results from these studies cannot be directly applied to small populations, since the population structure affects imputation accuracy. In addition, factors affecting imputation accuracy may also be intensified in small populations. Therefore, we aimed to compare different imputation strategies for the Portuguese Holstein cattle population considering several commercially available single nucleotide polymorphism (SNP) panels in a relatively small number of genotyped animals. Data from 1359 genotyped animals were used to evaluate imputation in 7 different scenarios. In the S1 to S6 scenarios, imputations were performed from LDv1, 50Kv1, 57K, 77K, HDv3 and Ax58K panels to 50Kv2 panel. In these scenarios, the bulls in 50Kv2 were divided into reference (352) and validation (101) populations based on the year of birth. In the S7 scenario, the validation population consisted of 566 cows genotyped with the Ax58K panel with their genotypes masked to LDv1. In general, all sample imputation accuracies were high with correlations ranging from 0.94 to 0.99 and concordance rate ranging from 92.59 to 98.18%. SNP-specific accuracy was consistent with that of sample imputation. S4 (40.32% of SNPs imputed) had higher accuracy than S2 and S3, both with less than 7.59% of SNPs imputed. Most probably, this was due to the high number of imputed SNPs with minor allele frequency (MAF) < 0.05 in S2 and S3 (by 18.43% and 16.06% higher than in S4, respectively). Therefore, for these two scenarios, MAF was more relevant than the panel density. These results suggest that genotype imputation using several commercially available SNP panels is feasible for the Portuguese national genomic evaluation. Keywords: dairy cattle, genomic evaluation, imputation accuracy Journal: Czech Journal of Animal Science Pages: 377-386 Volume: 64 Issue: 9 Year: 2019 DOI: 10.17221/120/2019-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/120/2019-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201909-0002.txt Handle: RePEc:caa:jnlcjs:v:64:y:2019:i:9:id:120-2019-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Roman Svejstil Author-Workplace-Name: Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Vladimir Plachy Author-Workplace-Name: Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Miroslav Joch Author-Workplace-Name: Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Workplace-Name: Department of Livestock Technology and Management, Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: Hana Salmonova Author-Workplace-Name: Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Dagmar Duskova Author-Workplace-Name: Department of Livestock Technology and Management, Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: Veerle Hautekiet Author-Workplace-Name: Huvepharma®, Antwerp, Belgium Author-Name: Eva Vlkova Author-Workplace-Name: Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Prague, Czech Republic Title: Effect of probiotic Clostridium butyricum CBM 588 on microbiota and growth performance of broiler chickens Abstract: Clostridium butyricum CBM 588 is used as a probiotic in eastern Asian countries and has been recently approved as an animal feed additive in the European Union. The purpose of this study was to evaluate the effect of C. butyricum MIYAIRI 588 (CBM 588) on abundance of selected genera of caecal and crop bacteria, volatile fatty acids and growth performance of broiler chickens. We studied counts of anaerobic bacteria in caeca and crops of broiler chickens by plate-count method and evaluated their growth performance. CBM 588 significantly reduced E. coli counts in caeca of broiler chickens at days 10 and 42 and also enhanced their growth performance. Additionally, it significantly increased the amount of butyrate in the caeca that provides energy to enterocytes, resulting in increased weight gains. Out of the obtained results we conclude that C. butyricum CBM 588 influences caecal microbiota of broiler chickens and positively affects their growth performance. Keywords: probiotic feed additive, E. coli, bifidobacteria, butyrate, feed efficiency Journal: Czech Journal of Animal Science Pages: 387-394 Volume: 64 Issue: 9 Year: 2019 DOI: 10.17221/143/2019-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/143/2019-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201909-0003.txt Handle: RePEc:caa:jnlcjs:v:64:y:2019:i:9:id:143-2019-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Laura Aline Zanelatto de Souza Author-Workplace-Name: Faculty of Agronomy and Animal Science, Federal University of Mato Grosso, Cuiabá, Brazil Author-Name: Heder José D'Avila Lima Author-Workplace-Name: Faculty of Agronomy and Animal Science, Federal University of Mato Grosso, Cuiabá, Brazil Author-Name: Renata Aparecida Martins Author-Workplace-Name: College of Veterinary Medicine and Animal Science, Sao Paulo State University, Botucatu, Brazil Author-Name: Andrey Sávio de Almeida Assunção Author-Workplace-Name: College of Veterinary Medicine and Animal Science, Sao Paulo State University, Botucatu, Brazil Author-Name: Donaldo Antônio Nunes Junior Author-Workplace-Name: Faculty of Agronomy and Animal Science, Federal University of Mato Grosso, Cuiabá, Brazil Author-Name: Willian Ferreira Silva Author-Workplace-Name: Faculty of Agronomy and Animal Science, Federal University of Mato Grosso, Cuiabá, Brazil Author-Name: Felipe Gomes da Silva Author-Workplace-Name: Faculty of Agronomy and Animal Science, Federal University of Mato Grosso, Cuiabá, Brazil Title: Egg yolk colour and retinol concentration of eggs from laying hens fed diets containing carrot and beetroot meal Abstract: Compounds such as pigments, antioxidants, and pro-vitamins A are found in carrots and beetroots and could increase the nutritional value of eggs when added to diets for laying hens. This research evaluated retinol concentrations and egg yolk colour with addition of freeze-dried carrot (Daucus carota L.) and beetroot (Beta vulgaris L.) meal in the diets of 240 Hisex Brown laying hens. The hens were distributed in a completely randomized design in five groups according to five experimental diets: (1) maize and soybean meal; (2) sorghum and soybean meal; (3) sorghum and soybean meal plus 0.8% of freeze-dried carrot meal; (4) sorghum and soybean meal plus 0.8% of freeze-dried beetroot meal; (5) sorghum and soybean meal plus 0.4% of freeze-dried carrot meal and 0.4% freeze-dried beetroot meal. The diet containing maize and soybean meal resulted in a more intense egg yolk colour and higher retinol levels in comparison to other diets (P < 0.05). However, carrot meal increased egg yolk colour and beetroot meal increased egg yolk retinol concentration in comparison to sorghum and soybean meal diets (P < 0.05). The inclusion of 0.8% of the carrot and beetroot meal in the diet is not enough to reach the intensity of yolk colour and yolk retinol concentration obtained through the diet containing maize, however, it increases retinol level and yolk colour in comparison to the diet containing sorghum without carrot and beetroot meal. Keywords: additive, Beta vulgaris L., Daucus carota L., sorghum, photography Journal: Czech Journal of Animal Science Pages: 395-403 Volume: 64 Issue: 9 Year: 2019 DOI: 10.17221/54/2019-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/54/2019-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-201909-0004.txt Handle: RePEc:caa:jnlcjs:v:64:y:2019:i:9:id:54-2019-CJAS