Template-Type: ReDIF-Article 1.0 Author-Name: Ruixia Lan Author-Workplace-Name: Department of Animal Science, College of Agriculture, Guangdong Ocean University, Zhanjiang, P.R. China Author-Name: Siqi Li Author-Workplace-Name: Department of Animal Science, College of Agriculture, Guangdong Ocean University, Zhanjiang, P.R. China Author-Name: Qingqing Chang Author-Workplace-Name: Department of Animal Science, College of Agriculture, Guangdong Ocean University, Zhanjiang, P.R. China Author-Name: Lilong An Author-Workplace-Name: Department of Animal Science, College of Agriculture, Guangdong Ocean University, Zhanjiang, P.R. China Author-Name: Zhihui Zhao Title: Sodium butyrate enhances growth performance and intestinal development in broilers Abstract: The aim of this study was to evaluate the effects of sodium butyrate (SB) on growth performance and development of digestive and immune organs in broilers. Dietary treatments had similar compositions but with 0%, 0.03%, 0.06%, and 0.12% SB substituted (weight/weight) for identical amounts of the basal diet. SB supplementation linearly increased (P < 0.05) average daily gain for each period, except for days 15-21. SB supplementation linearly increased (P < 0.05) the relative weight of proventriculus (day 7), gizzard (days 7 and 14), duodenum (days 21 and 28), jejunum (day 21), ileum (day 21), small intestine (day 21), rectum (day 14), pancreas (days 7 and 21), liver (days 21 and 28), and thymus (days 7, 14, and 21). SB supplementation linearly increased (P < 0.05) the relative length of duodenum (day 21), jejunum (days 14 and 21), ileum (days 14 and 21) and small intestine (days 14 and 21), caeca (day 21) and rectum (day 21), as well as it improved intestinal structure by increasing the villus height in jejunum and ileum, and increasing goblet cell counts in duodenum, jejunum, and ileum. Collectively, dietary SB supplementation improved the growth performance of broilers by improving the development and morphological structure of the broilers' intestinal organs. Keywords: relative weight, relative length, digestive organs, immune organs, broiler Journal: Czech Journal of Animal Science Pages: 1-12 Volume: 65 Issue: 1 Year: 2020 DOI: 10.17221/190/2019-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/190/2019-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202001-0001.txt Handle: RePEc:caa:jnlcjs:v:65:y:2020:i:1:id:190-2019-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Jindřich Čítek Author-Workplace-Name: University of South Bohemia in České Budějovice, Faculty of Agriculture, České Budějovice, Czech Republic Author-Name: Michaela Brzáková Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: Lenka Hanusová Author-Workplace-Name: University of South Bohemia in České Budějovice, Faculty of Agriculture, České Budějovice, Czech Republic Author-Name: Oto Hanuš Author-Workplace-Name: Dairy Research Institute, Prague, Czech Republic Author-Name: Libor Večerek Author-Workplace-Name: University of South Bohemia in České Budějovice, Faculty of Agriculture, České Budějovice, Czech Republic Author-Name: Eva Samková Author-Workplace-Name: University of South Bohemia in České Budějovice, Faculty of Agriculture, České Budějovice, Czech Republic Author-Name: Zuzana Křížová Author-Workplace-Name: University of South Bohemia in České Budějovice, Faculty of Agriculture, České Budějovice, Czech Republic Author-Name: Irena Hoštičková Author-Workplace-Name: University of South Bohemia in České Budějovice, Faculty of Agriculture, České Budějovice, Czech Republic Author-Name: Tereza Kávová Author-Workplace-Name: University of South Bohemia in České Budějovice, Faculty of Agriculture, České Budějovice, Czech Republic Author-Name: Karolina Straková Author-Workplace-Name: University of South Bohemia in České Budějovice, Faculty of Agriculture, České Budějovice, Czech Republic Author-Name: Lucie Hasoňová Author-Workplace-Name: University of South Bohemia in České Budějovice, Faculty of Agriculture, České Budějovice, Czech Republic Title: Technological properties of cow's milk: correlations with milk composition, effect of interactions of genes and other factors Abstract: We analysed the correlations of milk fermentability (yogurt test acidity), renneting (time to rennet coagulation of milk protein) and results of ethanol tests (indirect indicator of milk thermostability) with the percentages of the milk components. The correlations of the milk component percentages with renneting were mostly weak, while the correlations with the ethanol test were even poorer. The CSN3 and LGB genotypes did not show a significant interaction in their influence on milk fermentability, renneting or ethanol test results. For the ethanol test, many significant interactions were observed between the DGAT1, LEP, FASN, SCD1, CSN2, CSN3 and LGB genotypes. DGAT1 interacted significantly with all genes for milk fermentability. For renneting that was measured instrumentally, CSN3 interacted significantly with DGAT1, FASN, SCD1, CSN2 A/B, CSN2 A1/A2; SCD1 interacted significantly with LEP and FASN. The breed and genotypes did not show any interactions. Farm and genotypes interacted significantly for all the technological properties studied; similar results were observed for the protein percentage and the genotypes, except that no interactions influenced the ethanol test results. Keywords: casein, ethanol test, gene polymorphisms, milk fermentation, renneting Journal: Czech Journal of Animal Science Pages: 13-22 Volume: 65 Issue: 1 Year: 2020 DOI: 10.17221/150/2019-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/150/2019-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202001-0002.txt Handle: RePEc:caa:jnlcjs:v:65:y:2020:i:1:id:150-2019-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Heping Zhao Author-Workplace-Name: College of Animal Science, Henan University of Science and Technology, Luoyang, P.R. China Author-Name: Feike Zhang Author-Workplace-Name: Luoyang Xintai Agro-pastoral Technology Co., Ltd, Luoyang, P.R. China Author-Name: Jun Chai Author-Workplace-Name: Luoyang Polytechnic, Luoyang, P.R. China Author-Name: Jianping Wang Author-Workplace-Name: College of Animal Science, Henan University of Science and Technology, Luoyang, P.R. China Title: Effect of lactic acid bacteria on Listeria monocytogenes infection and innate immunity in rabbits Abstract: The present study aimed to investigate the effect of probiotic lactic acid bacteria (LAB) addition on Listeria monocytogenes translocation and its toxin listeriolysin O (LLO), proinflammatory factors, immune organ indexes and serum immunoglobulins in farmed rabbits. Five treatments included negative control (NC), positive control (PC) with L. monocytogenes infection and supplemental LAB at 3.0 × 106 (low-LAB, L-LAB), 3.0 × 108 (medium-LAB, M-LAB) and 3.0 × 1010 (high-LAB, H-LAB) CFU/kg of diet, respectively. The LAB was a mixture of equal amounts of Lactobacillus acidophilus (ACCC11073), Lactobacillus plantarum (CICC21863) and Enterococcus faecium (CICC20430). A total of 180 weaned rabbits (negative for L. monocytogenes) were randomly assigned to 5 groups with 6 replicates of 6 rabbits each in response to the 5 treatments. L. monocytogenes infection occurred on the first day of feeding trial and dietary LAB supplementation lasted for 14 days. The results showed that on days 7 and 14 post administration, L. monocytogenes in caecum, liver, spleen and lymph nodes was reduced in M-LAB and H-LAB compared to PC (P < 0.05), and linear and quadratic reducing trends were found in liver on day 7 (P ≤ 0.002). On day 14, mucosa LLO mRNA expression and serum TNFα, IL1β and IFNγ were reduced in the three LAB treatments (P < 0.05), and linear and quadratic trends were found on TNFα and IL1β (P ≤ 0.025); indexes of thymus and spleen, serum IgA and IgG were increased in the LAB treatments (P < 0.05). It is concluded that LAB can be used to alleviate L. monocytogenes infection and to improve the immune function of farmed animals. Keywords: immune organ, immunoglobulins, listeriolysin O, pathogen translocation, proinflammatory factor Journal: Czech Journal of Animal Science Pages: 23-30 Volume: 65 Issue: 1 Year: 2020 DOI: 10.17221/247/2019-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/247/2019-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202001-0003.txt Handle: RePEc:caa:jnlcjs:v:65:y:2020:i:1:id:247-2019-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Guang-Wei Ma Author-Workplace-Name: College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China Author-Workplace-Name: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, P.R. China Author-Name: Xin You Author-Workplace-Name: College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China Author-Workplace-Name: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, P.R. China Author-Name: Hua Yang Author-Workplace-Name: Institute of Animal Husbandry and Veterinary Medicine, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, P.R. China Author-Name: Xiao-Hong Yan Author-Workplace-Name: College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China Author-Workplace-Name: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, P.R. China Author-Name: Fang Mou Author-Workplace-Name: College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China Author-Workplace-Name: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, P.R. China Author-Name: Yan-Kai Chu Author-Workplace-Name: College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China Author-Workplace-Name: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, P.R. China Author-Name: En-Guang Rong Author-Workplace-Name: State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, P.R. China Author-Name: Shou-Zhi Wang Author-Workplace-Name: College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China Author-Workplace-Name: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, P.R. China Author-Name: Zhi-Peng Wang Author-Workplace-Name: College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China Author-Workplace-Name: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, P.R. China Author-Name: Hui Li Author-Workplace-Name: College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China Author-Workplace-Name: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, P.R. China Author-Name: Ning Wang Author-Workplace-Name: College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China Author-Workplace-Name: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, P.R. China Title: Polymorphisms and association of FAT1 gene with wool quality traits in Chinese Merino sheep Abstract: FAT atypical cadherin 1 (FAT1) is a member of the atypical cadherin superfamily and is involved in the planar cell polarity signalling pathway which regulates hair follicle morphogenesis, cycling, and orientation. The purpose of this study was to investigate the sheep FAT1 gene expression, polymorphisms and its association with wool quality traits in Chinese Merino sheep. Quantitative real-time PCR analysis showed that the FAT1 mRNA was differentially expressed in the adult skin of Chinese Merino and Kazak sheep. Seven SNPs (termed SNPs 1-7) were identified in exon 2 of sheep FAT1 gene by Sanger sequencing. SNPs 2 and 7 (novel SNPs) were significantly associated with wool crimp (P < 0.05). SNPs 4 and 5 (rs161528993 and rs161528992) were significantly associated with wool fibre length (P < 0.05). SNP 7 was highly significantly associated with average wool fibre diameter (P < 0.01). Similarly, FAT1 haplotypes were significantly associated with wool crimp (P < 0.05), and the haplotypes H1-H3 and H5 were significantly associated with higher wool crimp (P < 0.05). Bioinformatics analysis showed that the wool quality trait-associated SNPs (SNPs 2, 4, 5 and 7) might affect the pre-mRNA splicing by creating or abolishing the binding sites for serine/arginine-rich proteins, and in addition, SNPs 2 and 4 might alter the FAT1 mRNA secondary structure. Our results suggest that FAT1 influences wool quality traits and its SNPs 2, 4, 5 and 7 might be useful markers for marker-assisted selection and sheep breeding. Keywords: FAT atypical cadherin 1, tissue expression, SNP, wool crimp, wool fibre diameter Journal: Czech Journal of Animal Science Pages: 31-39 Volume: 65 Issue: 1 Year: 2020 DOI: 10.17221/224/2019-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/224/2019-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202001-0004.txt Handle: RePEc:caa:jnlcjs:v:65:y:2020:i:1:id:224-2019-CJAS