Template-Type: ReDIF-Article 1.0 Author-Name: Filipa Bubeníčková Author-Workplace-Name: Department of Veterinary Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Jiří Šichtař Author-Workplace-Name: Department of Veterinary Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Linda Nováčková Author-Workplace-Name: Department of Veterinary Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Jitka Sirohi Author-Workplace-Name: Department of Statistics, Faculty of Economy and Management, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Ondřej Šimoník Author-Workplace-Name: Department of Veterinary Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Title: The structure of subpopulations of stallion spermatozoa after thawing differs between good and poor freezers Abstract: The aim of this study was to evaluate differences in the presence of sperm subpopulations in frozen-thawed semen in stallions with different freezability. The motility of individual spermatozoa of 24 stallions from 15 breeds was evaluated using computer-assisted sperm analysis (CASA) immediately after thawing (T0) and after 30 min of incubation (T30). In accordance with our previous studies, samples were initially divided based on their total motility into categories of good (GF) and poor (PF) freezers. K-means cluster analysis of kinematic parameters of spermatozoa was used to divide motile sperm (n = 57 630) into three subpopulations. Analysis of variance was used to evaluate differences in the subpopulations between GF and PF stallions at the times of incubation T0 and T30. Statistically significant differences were found in most kinematic parameters between PF and GF stallions as well as between the times of incubation T0 and T30 (P < 0.05). Spermatozoa of good freezers are represented more frequently in the fast and medium fast subpopulations and are faster and more linear than those of poor freezers (P < 0.05). Sperm from PF stallions were more strongly affected by longer incubation. The percentage of sperm in the fast and medium fast subpopulations was lower in samples from PF stallions, but assessment of the motility parameters in particular sperm subpopulations revealed that these sperm had good velocity. Poor freezer samples had lower sperm quality due to a reduced total proportion of motile sperm, and these samples were more sensitive to prolonged time after thawing. Thus, an efficient sperm selection method or a special insemination technique should be used for obtaining doses from stallions with poor freezability. Our study showed that the CASA system and cluster analysis are promising tools for better understanding the significant differences in the individual stallion freezability, and further research should be focused on their application in the field. Keywords: reproduction, horse, cryopreservation, cluster analysis, CASA, fertility Journal: Czech Journal of Animal Science Pages: 403-410 Volume: 65 Issue: 11 Year: 2020 DOI: 10.17221/127/2020-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/127/2020-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202011-0001.txt Handle: RePEc:caa:jnlcjs:v:65:y:2020:i:11:id:127-2020-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Peter Loibl Author-Workplace-Name: TUM School of Live Sciences Weihenstephan, Chair for Animal Nutrition, Technical University of Munich, Freising-Weihenstephan, Germany Author-Name: Wilhelm Windisch Author-Workplace-Name: TUM School of Live Sciences Weihenstephan, Chair for Animal Nutrition, Technical University of Munich, Freising-Weihenstephan, Germany Author-Name: Wolfgang Preißinger Author-Workplace-Name: Institute for Animal Nutrition, Bavarian State Research Center for Agriculture, Grub, Germany Title: Examination of high-resolution feed intake data of growing-finishing pigs confronted with high deoxynivalenol contents present in their feed Abstract: Modern single space feeding systems for fattening pigs allow the detailed assessment of an individual animal's feeding behaviour. In an experiment involving 96 fattening pigs, the influence of deoxynivalenol (DON) contaminated feed (> 4 500 µg/kg DON) on the zootechnical performance and feeding behaviour was compared with a feed with low DON concentration (< 900 µg/kg DON), this served as the control group. Additionally, in separate treatments, two commercial mycotoxin binders were added to the DON-contaminated feeds to assess if an expected DON effect could be attenuated. The high DON content significantly (P < 0.03) reduced daily feed intake (500-600 g/day). The DON group showed 240 g less daily gain compared to the control with 728 g/day. Both mycotoxin binders were seen to additionally depress weight gain by approximately 65 g/day (P < 0.01). The treatment did not affect the individual feeding behaviour as assessed by daily visits to the feeder, feed intake per visit and the highest feed intake per visit per day. These were influenced only by the pig and its pen, indicating that the animals developed a distinct behaviour within their respective groups. Behaviour analyses of persistency and day-to-day variation showed that the persistency was reduced and variation was increased when DON without or with binder was present. The DON contents therefore seemed to lead to a more erratic and less consistent behaviour that remained dependent on the animal group. Keywords: behaviour, DON, fattening pig, mycotoxin binder Journal: Czech Journal of Animal Science Pages: 411-423 Volume: 65 Issue: 11 Year: 2020 DOI: 10.17221/189/2020-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/189/2020-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202011-0002.txt Handle: RePEc:caa:jnlcjs:v:65:y:2020:i:11:id:189-2020-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Michal Milerski Author-Workplace-Name: Department of Genetics and Breeding of Farm Animals, Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: Martin Ptáček Author-Workplace-Name: Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague-Suchdol, Czech Republic Author-Name: Jaromír Ducháček Author-Workplace-Name: Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague-Suchdol, Czech Republic Author-Name: Jitka Schmidová Author-Workplace-Name: Department of Genetics and Breeding of Farm Animals, Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: Michal Uhrinčať Author-Workplace-Name: National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Lužianky, Slovak Republic Author-Name: Luděk Stádník Author-Workplace-Name: Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague-Suchdol, Czech Republic Author-Name: Vladimír Tančin Author-Workplace-Name: National Agricultural and Food Centre, Research Institute for Animal Production Nitra, Lužianky, Slovak Republic Author-Workplace-Name: Department of Veterinary Science, Faculty of Agrobiology and Food Resources, Slovak Agricultural University in Nitra, Nitra, Slovak Republic Title: Analysis of the relationship between milk production, milk composition and morphological udder measurements in Wallachian sheep Abstract: The aim of this study was to evaluate the potential of udder measurements for prediction of milk yield, milk components and somatic cell count in non-dairy Wallachian sheep. The study was performed on 38 ewes in the Beskids Mountains kept under extensive management on pasture. Milk production (MILK) as well as samples for milk component and somatic cell count determination were collected during two separate control days (42nd day and 100th day of lactation on average). Rear udder depth (RUD, cm), udder width (UW, cm), and teat length (TL, mm) were measured at each control day as well. Linear regressions of udder measurement characteristics showed a predictive character ability for MILK only. An increasing of 70 g MILK corresponded with a 1 cm increase of RUD (P < 0.01) or 1 cm increase of UW (P < 0.001). These positive linear relationships were supported by the positive partial correlation analysis between MILK and RUD (r = 0.503; P < 0.001) or MILK and UW (r = 0.627; P < 0.001). An increase of 1 mm TL was associated with a 10 g (P < 0.01) increase of MILK; however, correlations between these traits were not significant. Results of this study demonstrate an alternative way of MILK ability in non-dairy Wallachian sheep population in general. Correlation and regression analysis further estimated this expected potential in detail using udder measurement characteristics. Measurement of RUD and UW could serve as a tool for breeding and flock management in order to maintain and improve milk production; however, there was no obvious evidence for the prediction of milk composition characteristics and somatic cell count. Keywords: ewe, milk yield, rear udder depth, udder width, teat length Journal: Czech Journal of Animal Science Pages: 424-430 Volume: 65 Issue: 11 Year: 2020 DOI: 10.17221/196/2020-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/196/2020-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202011-0003.txt Handle: RePEc:caa:jnlcjs:v:65:y:2020:i:11:id:196-2020-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Angelika Drozdova Author-Workplace-Name: Department of Animal Physiology and Ethology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic Author-Name: Zuzana Kankova Author-Workplace-Name: Department of Animal Physiology and Ethology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic Author-Name: Michal Zeman Author-Workplace-Name: Department of Animal Physiology and Ethology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic Author-Workplace-Name: Institute of Animal Biochemistry and Genetics, Centre of Biosciences, Slovak Academy of Sciences, Bratislava, Slovak Republic Title: Effect of cold and warm white light on selected endocrine and immune parameters of broiler embryos and hatchlings Abstract: Lighting conditions during incubation can influence embryonic development, post-hatching ontogeny and production efficiency. Previous studies revealed that different light colours differently affect pineal melatonin biosynthesis in embryos and postembryonic development of broiler chickens, but physiological mechanisms mediating these effects are not known. Cold and warm white light consists of different wavelengths and therefore the aim of the present study was to explore if these two lights can differently influence the development of circadian melatonin biosynthesis, production of thyroid hormones and corticosterone, concentration of metabolites (glucose, cholesterol and triacylglycerols) as well as expression of two important immune genes, presenilin 1 and avian beta-defensin 1 (AvBD-1). We evaluated these traits in embryos before hatching (456, 460, 465, 468 and 472 h of incubation) and in hatchlings. The rhythmic profile of pineal melatonin with higher concentrations during the dark time was determined in both treatment groups. Melatonin levels increased considerably in hatchlings in comparison with embryos, but we found no difference in rhythm characteristics between groups. We did not identify any daily rhythms in plasma corticosterone and thyroid hormone levels in either studied age and no differences were found between light treatments in concentrations of thyroid hormones, corticosterone, metabolites and expression of presenilin and AvBD-1. The expected developmental increase of thyroid hormones was proved. Gene expression of presenilin increased in the duodenum of hatchlings in comparison with embryos, but the expression did not change in the bursa of Fabricius. On the other hand, expression of AvBD-1 decreased in hatchlings compared to embryos in both tissues. Based on these results, we can conclude that the colour temperature of white light did not influence endocrine and immune parameters determined in this study and probably monochromatic rather than polychromatic light should be used to influence embryonic development and postembryonic ontogeny of broiler chickens. Keywords: chicken, incubation, polychromatic light Journal: Czech Journal of Animal Science Pages: 431-441 Volume: 65 Issue: 11 Year: 2020 DOI: 10.17221/230/2020-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/230/2020-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202011-0004.txt Handle: RePEc:caa:jnlcjs:v:65:y:2020:i:11:id:230-2020-CJAS