Template-Type: ReDIF-Article 1.0 Author-Name: Lenka Štohlová Putnová Author-Workplace-Name: Department of Animal Morphology, Physiology and Genetics, Faculty of AgriScience, Mendel University in Brno, Brno, Czech Republic Author-Name: Radek Štohl Author-Workplace-Name: Department of Control and Instrumentation, Faculty of Electrical Engineering and Communication, University of Technology, Brno, Czech Republic Title: The assignment success for 22 horse breeds registered in the Czech Republic: The machine learning perspective Abstract: The paper demonstrates the dependability of assignment testing in the identification of an appropriate breed to monitor comprehensive genetic information from molecular markers to analyse the collection of real population data covering 22 horse breeds registered in the Czech Republic, including native breeds and genetic resources. If 17 microsatellites are used, the mean number of alleles per locus corresponds to 10.4. The count of alleles at the individual loci ranges between five (HTG07) and 17 (ASB17). The loci ASB02, ASB23, HMS03, HTG10, and VHL20 exhibit the highest gene diversity and observed heterozygosity (both above 80%), with the mean value of 0.77 and 0.73, respectively. The moderate total inbreeding coefficient (5.2%) is estimated across all the loci and breeds. The levels of apparent breed differentiation span from zero between the Czech Warmblood and Slovak Warmblood to 0.15 between the Shetland Pony and Standardbred. The phylogenetic breed relationships are revealed via the NeighbourNet dendrogram constructed from Reynolds' genetic distances, which clearly separate the Coldblood draught, Hot/Warmblood, and Pony horses. Our results reveal that the Bayesian approach (the Rannala and Mountain technique) provides the most intensive prediction power (83.6%) out of the GeneClass tools and that the Bayes Net algorithm exhibits the best efficiency (78.4%) from the WEKA machine learning workbench options, considering the use of the five-fold cross-validation technique. The algorithms could be trained on large real reference data sets, and thus there appears another viable perspective for machine learning in horse ancestry testing. In this context, it is also important to stress the fact that innovated computational tools will potentially lead towards structuring a novel webserver to allow the identification of horse breeds. Keywords: accuracy, GeneClass analyses, individual breed assignment, DNA markers, WEKA algorithms Journal: Czech Journal of Animal Science Pages: 1-12 Volume: 66 Issue: 1 Year: 2021 DOI: 10.17221/120/2020-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/120/2020-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202101-0001.txt Handle: RePEc:caa:jnlcjs:v:66:y:2021:i:1:id:120-2020-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Yotsapon Yangngam Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand Author-Name: Suttiwat Chapanya Author-Workplace-Name: Lumphayaklang Livestock Semen Production Center, Lopburi, Thailand Author-Name: Thevin Vongpralub Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand Author-Name: Wuttigrai Boonkum Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand Author-Workplace-Name: Network Center for Animal Breeding and Omics Research, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand Author-Name: Vibuntita Chankitisakul Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand Author-Workplace-Name: Network Center for Animal Breeding and Omics Research, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand Title: Effect of semen extender supplementation with sericin on post-thaw dairy bull sperm quality and lipid peroxidation Abstract: Silk sericin plays a protective role in a variety of mammalian cells during cryopreservation. This study aimed to determine the effects of sericin supplementation to a freezing extender on sperm quality and lipid peroxidation in dairy bull sperm cryopreservation. Each semen sample of five Holstein-Friesian crossbred bulls was divided into four aliquots and diluted in a tris-egg yolk extender supplemented with different concentrations of sericin [0%, 0.25%, 0.5%, and 1.0% (wt/vol)]. Sperm motility (CASA), viability, acrosome integrity, mitochondrial membrane potential (fluorescent staining) and lipid peroxidation (malondialdehyde - MDA test) were analysed. The results show that the 0.25% and 0.5% sericin groups had the highest total sperm motility (P < 0.05). Sperm viability, acrosome integrity, and mitochondrial function were higher in the group supplemented with 0.25% sericin compared to the control and 1.0% (P < 0.05). Sericin supplementation with 0.25% and 0.5% significantly decreased MDA concentrations compared with the control (P < 0.05). In conclusion, supplementation of the semen freezing extender with sericin at the concentration of 0.25% significantly improved the post-thaw semen quality and reduced lipid peroxidation in Holstein-Friesian crossbred bulls. Keywords: antioxidant, cattle, frozen semen, ROS, semen cryopreservation Journal: Czech Journal of Animal Science Pages: 13-20 Volume: 66 Issue: 1 Year: 2021 DOI: 10.17221/104/2020-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/104/2020-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202101-0002.txt Handle: RePEc:caa:jnlcjs:v:66:y:2021:i:1:id:104-2020-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Panida Chanapiwat Author-Workplace-Name: Semen Laboratory, Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Thailand Author-Name: Kampon Kaeoket Title: l-cysteine prolonged fresh boar semen qualities, but not for docosahexaenoic acid Abstract: The present study aimed to develop a long-term fresh boar semen extender by adding l-cysteine and docosahexaenoic acid (DHA) into Beltsville thawing solution (BTS)-based extender. Semen samples were collected from six boars, diluted at a concentration of 3 × 109 spermatozoa/100 ml and allocated into 10 groups as follows: BTS, Merck III®, Androstar® Plus, ModenaTM, DHA 1.5 mg/ml, 3 mg/ml, and 6.25 mg/ml, l-cysteine 2.5 mM, 5.0 mM, and 10 mM. All extended semen samples were stored at 18 °C and were evaluated for progressive motility, viability, acrosome integrity, pH, and osmolality on days 0, 1, 3, 5, and 7 of storage. The results documented that on day 3, l-cysteine at 2.5 mM showed the significantly highest percentage (78.8%) of progressive motility, but not different from ModenaTM (78.8%) and Androstar® Plus (71.3%) (P < 0.05). On day 5, l-cysteine at 2.5 mM maintained progressive motility and viability up to 73.8% and 77.9%, respectively, but not different from ModenaTM (78.8% and 74.8%) and Androstar® Plus (70.0% and 76.8%) (P < 0.05). On day 7, superior progressive motility (71.3%, 77.5% and 72.5%) and viability (72.8%, 77.3% and 69.8%) were found for l-cysteine at 2.5 mM, ModenaTM and Androstar® Plus groups, respectively, when compared to the other groups (P < 0.01). However, in the present study, DHA supplementation failed to maintain fresh boar semen qualities during storage. In conclusion, adding l-cysteine at a concentration of 2.5 mM in a BTS-based extender is the optimal concentration for the preservation of fresh boar semen at 18 °C for seven days. Keywords: antioxidant, artificial insemination, DHA, pig, semen extender, spermatozoa Journal: Czech Journal of Animal Science Pages: 21-28 Volume: 66 Issue: 1 Year: 2021 DOI: 10.17221/199/2020-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/199/2020-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202101-0003.txt Handle: RePEc:caa:jnlcjs:v:66:y:2021:i:1:id:199-2020-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Júlia Marixara Sousa da Silva Author-Workplace-Name: Federal Institute of Goiano, Rio Verde Campus, Rio Verde, Brazil Author-Name: Nayanne Rodrigues de Oliveira Author-Workplace-Name: Federal Institute of Goiano, Rio Verde Campus, Rio Verde, Brazil Author-Name: Alison Batista Vieira Silva Gouveia Author-Workplace-Name: Federal Institute of Goiano, Rio Verde Campus, Rio Verde, Brazil Author-Name: Rodolfo Alves Vieira Author-Workplace-Name: Federal University of Viçosa, Viçosa, Brazil Author-Name: Ronan Omar Fernandes dos Santos Author-Workplace-Name: BRF S.A., Rio Verde, Brazil Author-Name: Cibele Silva Minafra Author-Workplace-Name: Federal Institute of Goiano, Rio Verde Campus, Rio Verde, Brazil Author-Name: Fabiana Ramos dos Santos Author-Workplace-Name: Federal Institute of Goiano, Rio Verde Campus, Rio Verde, Brazil Title: Effect of protease supplementation on the digestibility of amino acids in animal-origin meals for broiler diets Abstract: Enzymes benefit digestion and absorption of the ingredients and their addition to an animal-origin meal (AOM) can improve its nutritional quality. This research aimed to evaluate the effect of protease on nutrient digestibility, amino acids, and metabolism of AOM energy for broilers. Four hundred and eighty broiler chickens were distributed in a completely randomized design (4 × 2 factorial scheme), eight treatments, six replicates containing 10 birds/replicate. Treatments consisted of poultry viscera meal, swine viscera meal (SVM), bovine meat and bone meal, and basal diet; with and without protease addition. Two tests were performed. In the first test, the total excreta collection method was used with birds at 13 to 20 days of age and 25% of the reference feed was replaced by AOM. In the second test, a protein-free diet was administered to birds at 21 to 24 days of age and AOM replaced 25% of the starch. The inclusion of protease increased the apparent metabolizable energy corrected for nitrogen balance of SVM by 15.99% and the apparent metabolizable crude energy by 5.7%, and it also raised the coefficient of true ileal digestibility of the amino acids in the AOMs by 5.67% on average. The inclusion of protease improved the apparent metabolizable crude energy of AOMs, apparent metabolizable dry matter of bovine meat and bone meal, coefficient of true ileal digestibility of essential amino acids, and apparent metabolizable energy corrected for nitrogen balance of SVM. Dietary supplementation of protease may be a potential strategy to improve the digestibility of amino acids for broilers, a possibility of using animal-origin meals as a protein source of diets. Keywords: additive, by-product, digestion, exogenous enzymes, nutrition, poultry Journal: Czech Journal of Animal Science Pages: 29-37 Volume: 66 Issue: 1 Year: 2021 DOI: 10.17221/134/2020-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/134/2020-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202101-0004.txt Handle: RePEc:caa:jnlcjs:v:66:y:2021:i:1:id:134-2020-CJAS