Template-Type: ReDIF-Article 1.0 Author-Name: Isabel Cristina Acosta-Balcazar Author-Workplace-Name: Colegio de Postgraduados, Campus Tabasco, México Author-Name: Jorge Quiroz-Valiente Author-Workplace-Name: Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Campo Experimental Huimanguillo, Tabasco, México Author-Name: Lorenzo Granados-Zurita Author-Workplace-Name: Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Campo Experimental Huimanguillo, Tabasco, México Author-Name: Emilio Manuel Aranda-Ibáñez Author-Workplace-Name: Colegio de Postgraduados, Campus Tabasco, México Author-Name: Edith Hernández-Nataren Author-Workplace-Name: Colegio de Postgraduados, Campus Tabasco, México Author-Name: Joaquín Alberto Rincón-Ramírez Author-Workplace-Name: Colegio de Postgraduados, Campus Tabasco, México Author-Name: Lorenzo Danilo Granados-Rivera Author-Workplace-Name: Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Campo Experiemental General Terán, Nuevo León, México Title: Effect of genotype, lactation and climatic factors on fatty acid profile of bovine milk Abstract: Milk fat from bovine milk contains fatty acids that may have favourable properties for human health, for example, conjugated linoleic acid (CLA) has nutraceutical activity. This research aimed to know the effects of genotype, days of lactation and climatic factors on the fatty acids (FA) profile of milk and particularly the content of CLA in milk fat. Seventeen first-calving milking cows in early lactation were used for the assessment of milk; 12 were Gyr and five were F1 (Holstein/Gyr) crosses. Sampling was carried out every 15 days, from the beginning to the end of lactation (300 days). Fatty acids were analyzed employing gas chromatography. The genotype did not influence the content of the fatty acid groups: saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA) and CLA. The highest MUFA and PUFA contents were recorded at 100 days of lactation (32.334 and 3.553 g/100 g of FA, respectively), while SFA and CLA had their highest production at 200 days of lactation (63.238 and 1.378 g/100 g of FA, respectively). Regarding the climate, the highest temperature caused a decrease in the CLA content, because temperatures above 30 °C caused a decrease in the grazing time. Keywords: CLA, Gyr, temperature, tropics Journal: Czech Journal of Animal Science Pages: 167-175 Volume: 67 Issue: 5 Year: 2022 DOI: 10.17221/32/2022-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/32/2022-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202205-0001.txt Handle: RePEc:caa:jnlcjs:v:67:y:2022:i:5:id:32-2022-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Radko Loučka Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: Václav Jambor Author-Workplace-Name: NutriVet, Ltd, Pohořelice, Czech Republic Author-Name: Jan Nedělník Author-Workplace-Name: Agricultural Research, Ltd Troubsko, Czech Republic Author-Name: Jaroslav Lang Author-Workplace-Name: Agricultural Research, Ltd Troubsko, Czech Republic Author-Name: Petr Homolka Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Workplace-Name: Deparment of Microbiology, Nutrition and Dietetics; Faculty of Agrobiology, Food and Natural Resources; Czech University of Life Sciences, Prague, Czech Republic Author-Name: Filip Jančík Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: Veronika Koukolová Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: Petra Kubelková Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: Yvona Tyrolová Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Author-Name: Alena Výborná Author-Workplace-Name: Institute of Animal Science, Prague-Uhříněves, Czech Republic Title: Differences between chemical analysis and portable near-infrared reflectance spectrometry in maize hybrids Abstract: The aim of this study is to compare the differences between four maize hybrids in terms of nutrient determination by portable near-infrared reflectance spectrometer (pNIRS) and chemical analysis; each of the hybrids was grown in the same locality from 2018 to 2020. The topic relates to the variability of the feed value of maize being an important feedstuff in livestock nutrition. The nutritional values determined by pNIRS in comparison with the chemical analysis were higher (P < 0.001) in starch and ash content but lower in dry matter, neutral detergent fibre and crude protein (CP) content. The digestibility levels of neutral detergent fibre and the net energy of lactation as well as the potential milk production per hectare in relation to each tonne of dry matter were also lower. According to this result, it would be necessary to calibrate all tested indicators for a given spectrometer. However, the pNIRS results are useful for evaluating nutrient variability; the standard deviation of the values found in pNIRS was mostly lower than that determined chemically. The pNIRS results are also useful for making practical adjustments to the total mixed rations when calculated from actual chemical analysis if the correlation between the two methods is used; the correlation between the pNIRS and chemical results was found to be significant (P < 0.05) in terms of all the indicators. Keywords: forage species, product quality, ruminant nutrition, digestibility, potential milk production Journal: Czech Journal of Animal Science Pages: 176-184 Volume: 67 Issue: 5 Year: 2022 DOI: 10.17221/23/2022-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/23/2022-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202205-0002.txt Handle: RePEc:caa:jnlcjs:v:67:y:2022:i:5:id:23-2022-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Guosheng Xin Author-Workplace-Name: School of Life Science, Ningxia University, Yinchuan, P.R. China Author-Workplace-Name: Ningxia Feed Engineering Technology Research Center, Ningxia University, Yinchuan, P.R. China Author-Name: Ju Guo Author-Workplace-Name: School of Agriculture, Ningxia University, Yinchuan, P.R. China Author-Name: Baojun Yu Author-Workplace-Name: School of Agriculture, Ningxia University, Yinchuan, P.R. China Author-Name: Hao Li Author-Workplace-Name: School of Life Science, Ningxia University, Yinchuan, P.R. China Author-Workplace-Name: Ningxia Feed Engineering Technology Research Center, Ningxia University, Yinchuan, P.R. China Author-Name: Yaling Gu Author-Workplace-Name: School of Agriculture, Ningxia University, Yinchuan, P.R. China Author-Name: Qiaoxian Gao Author-Workplace-Name: School of Life Science, Ningxia University, Yinchuan, P.R. China Author-Workplace-Name: Ningxia Feed Engineering Technology Research Center, Ningxia University, Yinchuan, P.R. China Author-Name: Juan Zhang Author-Workplace-Name: School of Agriculture, Ningxia University, Yinchuan, P.R. China Title: Effect of dietary Sophora alopecuroides supplementation on differential expression of intramuscular fat-related genes of Ningxia Tan sheep as determined by transcriptome sequencing Abstract: The present study investigated the effect of different dietary levels (0%, 0.25%, 0.5%, 1%, 2%) of Sophora alopecuroides (S. alopecuroides) on intramuscular fat (IMF) content in Ningxia Tan sheep. Candidate genes affecting IMF deposition were screened by comparing the RNA-Seq profiles of test (2% S. alopecuroides) and control (no S. alopecuroides) groups. The results showed that the intramuscular fat content of the diet supplemented with S. alopecuroides was significantly lower than that of the control group, and the intramuscular fat content decreased significantly with the increase of the dietary content of S. alopecuroides (P < 0.05). The highest IMF content of the control group (group A) was 4.21%, which was significantly higher than that of the other four experimental groups (B, C, D, E) (P < 0.05). Moreover, 66 genes were significantly upregulated and 202 genes were significantly downregulated in the test group compared to the control. Gene Ontology functional annotation revealed that the differentially expressed genes were closely related to plasma lipoprotein particle assembly, plasma lipoprotein particle organization, protein-lipid complex subunit organization, and other biological processes. KEGG pathway analysis showed that the differentially expressed genes belonged to 147 metabolic pathways. Finally, four differential genes which may be related to IMF deposition were screened out in PPAR signalling pathway, fatty acid biosynthesis, fatty acid degradation, and fatty acid metabolism. The quantitative analysis of four differential genes including ACSL3, PLIN2, ABCA1 and ANGPTL4 was carried out by RT-qPCR method, and the results were basically consistent with those of RNA-seq. Among them, the quantitative results of PLIN2 gene were slightly different from the sequencing results, but the difference was not significant, and they may be false positive. This study lays the foundation for understanding the molecular mechanism regulating mutton quality, and provides a theoretical basis for the study of S. alopecuroides as a feed additive to improve mutton meat quality and impact the gene function. Keywords: S. alopecuroides, RT-qPCR, IMF deposition, RNA-Seq Journal: Czech Journal of Animal Science Pages: 185-197 Volume: 67 Issue: 5 Year: 2022 DOI: 10.17221/127/2021-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/127/2021-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202205-0003.txt Handle: RePEc:caa:jnlcjs:v:67:y:2022:i:5:id:127-2021-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Martin Kutlvašr Author-Workplace-Name: Department of Animal Husbandry, Animal Nutrition and Biochemistry, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: Eva Straková Author-Workplace-Name: Department of Animal Husbandry, Animal Nutrition and Biochemistry, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: Pavel Suchý Author-Workplace-Name: Department of Animal Husbandry, Animal Nutrition and Biochemistry, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: Lucie Všetičková Title: Effect of partial and complete substitution of lupin meal for soybean meal in diets on changes in fatty acid composition of muscle fat in broiler chickens Abstract: The experiment aimed to determine the effect of 50% (LS50) or 100% (LS100) substitution of lupin protein (variety Zulika) for soybean protein in feed mixtures intended for the nutrition of broiler chickens, on muscular fat quality (composition of fatty acids). There were established three groups of chickens for fattening with 80 individuals each (control group C and experimental groups). After the 34-day fattening period chicken breast and thigh muscles were analysed to find out the fatty acid composition. Lupin protein-based diets had a positive effect on the muscle quality of fattened chickens due to changes in fatty acid composition, compared to soya protein-based diets. The feeding of lupin-based diets to broiler chickens resulted in the reduction of saturated fatty acids (P ≤ 0.05) by 14% in LS50 group and 17% in LS100 group and increase of unsaturated fatty acids (P ≤ 0.05) by 58% in LS50 group and 90% in LS100 group in muscle fat. The results clearly confirm that lupin-based diet increases the dietary value of chicken meat as one of the most important protein sources in human nutrition. Keywords: broilers, fattening, meat quality, UFA, SFA Journal: Czech Journal of Animal Science Pages: 198-207 Volume: 67 Issue: 5 Year: 2022 DOI: 10.17221/56/2022-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/56/2022-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202205-0004.txt Handle: RePEc:caa:jnlcjs:v:67:y:2022:i:5:id:56-2022-CJAS