Template-Type: ReDIF-Article 1.0 Author-Name: Jindřich Čítek Author-Workplace-Name: Department of Genetics and Agricultural Biotechnology, Faculty of Technology and Agriculture, South Bohemia University, Ceske Budejovice, Czech Republic Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: Libor Večerek Author-Workplace-Name: Department of Genetics and Agricultural Biotechnology, Faculty of Technology and Agriculture, South Bohemia University, Ceske Budejovice, Czech Republic Author-Name: Soňa Šlosárková Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: Petr Fleischer Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: Luboš Vostrý Author-Workplace-Name: Czech University of Life Sciences, Prague, Czech Republic Author-Name: Hana Vostrá-Vydrová Author-Workplace-Name: Czech University of Life Sciences, Prague, Czech Republic Author-Workplace-Name: Institute of Animal Science, Prague, Czech Republic Author-Name: Daniela Schröffelová Author-Workplace-Name: Czech Moravian Breeder´s Corporation Inc., Hradištko, Czech Republic Author-Name: Josef Kučera Author-Workplace-Name: Czech Moravian Breeder´s Corporation Inc., Hradištko, Czech Republic Title: Long-term pedigree analysis: An effective tool for managing congenital malformations in cattle Abstract: Controlling congenital defects is an important aspect of breeding for genetic health; however, whether malformations are caused by genetic or nongenetic factors may not be clear. This study aims to analyse the incidence of aplasia genitalis, atresia ani et recti and hernia cerebralis in the Czech cattle population, analyse the relatedness of affected calves by a relationship matrix, and assess the potential future threat. The sires fathering affected calves were born in the Czech Republic or imported from 1986 to 2001. The cases occurred on farms across the Czech Republic. The pedigree of each case was examined for common maternal and paternal ancestors (inbreeding loops) and for ancestors shared by other cases of the defect. The average relatedness coefficient of each individual was computed based on the relationship matrix. The results of the analysis of pedigrees and of the relationship matrix support the hereditary aetiology. The 13 calves affected by aplasia genitalis had common ancestors in 12 cases. The results indicate hereditary causation with recessive inheritance. In atresia ani et recti, some of the pedigrees of 25 affected calves support hereditary causation, and repeating ancestors were found for 11 calves. Our analysis of 11 hernia cerebralis cases also hints at the genetic background, but not as unequivocally as in other congenital defects studied. A high number of descendants fathered by sires of affected calves constitutes a risk for future. The relationship matrix and pedigree analysis could help in managing genetic health, although the final goal in terms of inherited defects must be the description of causal genes and mutations. Measures to control sires and dams with affected calves should be appropriate under the current knowledge, which include culling or prudent use of breeding with the monitoring of descendants. Keywords: genetic health, surveillance program, aplasia genitalis, atresia ani et recti, hernia cerebralis Journal: Czech Journal of Animal Science Pages: 385-393 Volume: 67 Issue: 10 Year: 2022 DOI: 10.17221/70/2022-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/70/2022-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202210-0001.txt Handle: RePEc:caa:jnlcjs:v:67:y:2022:i:10:id:70-2022-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Ye Wang Author-Workplace-Name: School of Biological and Food Processing Engineering, Huanghuai University, Zhumadian, P.R. China Author-Workplace-Name: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, P.R. China Author-Name: Chaoying Liu Author-Workplace-Name: School of Biological and Food Processing Engineering, Huanghuai University, Zhumadian, P.R. China Author-Workplace-Name: Zhumadian Academy of Industry Innovation and Development, Zhumadian, P.R. China Author-Name: Jinjin Zhu Author-Workplace-Name: School of Biological and Food Processing Engineering, Huanghuai University, Zhumadian, P.R. China Author-Name: Enzhong Li Author-Workplace-Name: School of Biological and Food Processing Engineering, Huanghuai University, Zhumadian, P.R. China Author-Name: Mingcheng Wang Author-Workplace-Name: School of Biological and Food Processing Engineering, Huanghuai University, Zhumadian, P.R. China Author-Name: Zhichao Shen Author-Workplace-Name: Ruyang County Agricultural Product Quality and Safety Inspection Station, Luoyang, P.R. China Author-Name: Xinfeng Yang Author-Workplace-Name: School of Biological and Food Processing Engineering, Huanghuai University, Zhumadian, P.R. China Author-Name: Guojin Lv Author-Workplace-Name: Sheqi Jingou Agriculture and Animal Husbandry Co., Ltd, Nanyang, P.R. China Author-Name: Rongfu Guo Author-Workplace-Name: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, P.R. China Title: Impact of different dietary fibre levels on the roughage resistance of the Dahe black pig Abstract: To investigate the effects and molecular mechanism of different levels of dietary fibre (Chinese milk vetch) on Dahe black and Duroc × (Landrace × Yorkshire) (DLY) pigs, 54 of each type of pig were selected and fed diets that contained 3.5%, 5.5% and 7.5% levels of fibre in the growing (30-60 kg) and fattening stages (60-120 kg). The production performance, serum physicochemical indices, nutrient digestibility, the amylase activity in the small intestine, contents of the muscle crude protein and ether extract, and the levels of transcription of the PRKAG3 and Fsp27 genes were determined. The production performance of the Dahe black pigs with a 5.5% level of dietary fibre was significantly higher than those with dietary fibre levels of 3.5% and 7.5%. A diet high in fibre also significantly affected the production performance of the DLY pigs. The apparent digestibility of the nutrients decreased with an increase in the dietary fibre level, and the Dahe black pigs appeared to more effectively digest the dietary fibre than the DLY pigs. The serum physicochemical indices, amylase activity, and the expression levels of the PRKAG3 and Fsp27 genes from the Dahe black pigs were significantly higher than those of the DLY pigs, and the intramuscular fat content of the Dahe black pigs fed a high fibre diet was significantly higher than that of the DLY pigs. The dietary fibre levels of 5.5% and 7.5% did not affect the production performance of the Dahe black pigs during the 30-120 kg period, but they significantly reduced the production performance of the DLY pigs. The Dahe black pigs were evidently tolerant to high amounts of fibre when fed a high fibre diet. Keywords: production performance, nutrient digestibility, PRKAG3, Fsp27, Chinese milk vetch, gene expression Journal: Czech Journal of Animal Science Pages: 394-406 Volume: 67 Issue: 10 Year: 2022 DOI: 10.17221/63/2022-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/63/2022-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202210-0002.txt Handle: RePEc:caa:jnlcjs:v:67:y:2022:i:10:id:63-2022-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Panida Chanapiwat Author-Workplace-Name: Semen Laboratory, Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon-pathom, Thailand Author-Name: Shutipen Buranasinsup Author-Workplace-Name: Department of Pre-Clinical Sciences and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakhon-pathom, Thailand Author-Name: Kampon Kaeoket Author-Workplace-Name: Semen Laboratory, Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon-pathom, Thailand Title: Transformation of a short-term boar semen extender into a long-term boar semen extender by using penicillamine Abstract: This study aimed to convert a short-term semen extender into a long-term semen extender by adding penicillamine (PEN). Semen samples were collected from six boars, diluted at a concentration 3 × 109 spermatozoa/100 ml, and divided into eight groups as follows: BTS, Merck III®, Androstar® Plus, PEN 0.125 mM, PEN 0.25 mM, PEN 0.5 mM, PEN 1.0 mM, PEN 2.0 mM. All the diluted semen samples were stored at 18 °C and were examined for progressive motility, viability, acrosome integrity, pH and osmolarity on days 0, 1, 3, 5 and 7 after storage. The level of the total anti-oxidative capability (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) were also measured for antioxidant activities. Fertility tests on a commercial pig farm was also conducted. On days 0, 1 and 3 of storage, no statistically significant differences in all the parameters among the extenders was observed. On day 5, the semen diluted with PEN at a concentration of 0.25 mM and Androstar® Plus could maintain progressive motility reach of 70.0% and 76.0%, respectively (P < 0.01). On day 7, the semen diluted with PEN at a concentration of 0.25 mM maintained progressive motility (70.0%) and acrosome integrity (85.7%) which is comparable to long-term semen extenders (Androstar® Plus) (P < 0.01). For the antioxidant activities, the level of the T-AOC, SOD and MDA varied depended on the PEN concentrations . The results of the fertility test were not different among the groups. To conclude, adding PEN at 0.25 mM in the BTS extender is able to transform a short-term semen extender into a long-term extender. Keywords: antioxidant activity, artificial insemination, pig, reproduction, sperm Journal: Czech Journal of Animal Science Pages: 407-415 Volume: 67 Issue: 10 Year: 2022 DOI: 10.17221/130/2022-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/130/2022-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202210-0003.txt Handle: RePEc:caa:jnlcjs:v:67:y:2022:i:10:id:130-2022-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Sailuo Wan Author-Workplace-Name: College of Agricultural Engineering, Anhui Open University, Hefei, P.R. China Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China Author-Name: Qianqian Pan Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China Author-Workplace-Name: Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei, P.R. China Author-Name: Jinbo Wei Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China Author-Workplace-Name: Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei, P.R. China Author-Name: Cuiyun Zhu Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China Author-Workplace-Name: Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei, P.R. China Author-Name: Jing Jing Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China Author-Workplace-Name: Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei, P.R. China Author-Name: Shuaiqi Qin Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China Author-Workplace-Name: Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei, P.R. China Author-Name: Rongcui Hu Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China Author-Workplace-Name: Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei, P.R. China Author-Name: Mengyu Lou Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China Author-Workplace-Name: Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei, P.R. China Author-Name: Shuang Li Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China Author-Workplace-Name: Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei, P.R. China Author-Name: Yinghui Ling Author-Workplace-Name: College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China Author-Workplace-Name: Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei, P.R. China Title: Isolation, culture and identification of sheep skeletal muscle satellite cells Abstract: Skeletal muscle satellite cells (SMSCs) play an important role in muscle growth, regeneration and maintenance. This study aimed to isolate SMSCs from sheep, establish a system for isolation, culture and identification of SMSCs in vitro, and provide seed cells for subsequent studies. SMSCs were isolated and purified from newborn 2-day-old healthy sheep by collagenase type I and trypsin two-step digestion and pre-plating method. The results indicated that the isolated and purified SMSCs showed full spindle shape and strong refractive index. The cell growth curve detected by CCK-8 kit was typical "S" type. Immunofluorescence analysis showed that the isolated cells expressed SMSC marker proteins Pax7 and MyoD1. After induction of myogenic differentiation, the cells fused with each other to form multinucleated myotubes and expressed the myoblast specific marker MHC. RT-PCR results showed that the cells expressed SMSC marker gene Pax 7. This experiment established an in vitro isolation, purification and identification system for sheep skeletal muscle satellite cells, which provided a good cell model for studying the biological mechanism of sheep skeletal muscle cells, optimizing sheep breeds, and cell transplantation repair. Keywords: SMSC, proliferation, myogenic differentiation, identification Journal: Czech Journal of Animal Science Pages: 416-423 Volume: 67 Issue: 10 Year: 2022 DOI: 10.17221/52/2022-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/52/2022-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202210-0004.txt Handle: RePEc:caa:jnlcjs:v:67:y:2022:i:10:id:52-2022-CJAS