Template-Type: ReDIF-Article 1.0 Author-Name: Martin Ptáček Author-Workplace-Name: Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Michal Milerski Author-Workplace-Name: Genetics and breeding of Farm Animals, Institute of Animal Science, Prague, Czech Republic Author-Name: Tereza Michlová Author-Workplace-Name: Department of Chemistry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Jaromír Ducháček Author-Workplace-Name: Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Vladimír Tančin Author-Workplace-Name: Research Institute for Animal Production, National Agriculture and Food Centre, Nitra, Slovak Republic Author-Workplace-Name: Institute of Animal Husbandry, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic Author-Name: Michal Uhrinčať Author-Workplace-Name: Research Institute for Animal Production, National Agriculture and Food Centre, Nitra, Slovak Republic Author-Name: Jitka Schmidová Author-Workplace-Name: Genetics and breeding of Farm Animals, Institute of Animal Science, Prague, Czech Republic Author-Name: Filipp Georgijevič Savvulidi Author-Workplace-Name: Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Luděk Stádník Author-Workplace-Name: Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Title: Monitoring of milk performance of Wallachian sheep grazed under traditional Carpathian management in Western Beskids location Abstract: This study aimed to determine the potential of Wallachian sheep in quantitative and qualitative indicators of milk production under extensive conditions of the Western Beskids mountains. Milk samples were collected from the group of 38 non-dairying sheep selected from the basic 120-head flock. Ewes were monitored during four control days (from 27th April, the average 42nd day of lactation to 4th August, the average 142nd day of lactation) to cover the whole lactation period. Ewes were investigated for their milk production and for milk quantity parameter. Milk quality indicators included percentage estimation of milk components (fat, protein, casein, lactose, dry matter), somatic cell count (103 cells/ml), vitamin A and E content (mg/kg), and K, Mg, Ca, Zn, Cu (mg/kg) content. Milk production ranged from 1 017.37 g (early stage of lactation) to 416.87 g (late stage of lactation period). The milk contained high fat (6.06-8.44%) and protein (4.68-5.68%) percentages, low somatic cell count and favourable distribution of minerals and vitamins. In general, results of this study indicate a possibility for low-productive traditional grazing system with extensive sheep breeds not only for cultivating the area but also for the purpose of high nutritional food with beneficial aspect on human health. Keywords: extensive grazing, genetic resource, mineral and vitamin content, somatic cell count Journal: Czech Journal of Animal Science Pages: 460-468 Volume: 68 Issue: 11 Year: 2023 DOI: 10.17221/85/2023-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/85/2023-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202311-0001.txt Handle: RePEc:caa:jnlcjs:v:68:y:2023:i:11:id:85-2023-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Eva Kašná Author-Workplace-Name: Institute of Animal Science, Prague, Czech Republic Author-Name: Ludmila Zavadilová Author-Workplace-Name: Institute of Animal Science, Prague, Czech Republic Author-Name: Zuzana Krupová Author-Workplace-Name: Institute of Animal Science, Prague, Czech Republic Author-Name: Soňa Šlosárková Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: Petr Fleischer Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: The most common reproductive disorders of cows in Holstein cattle breeding Abstract: The paper deals with the genomic evaluation of retained placenta (RP), metritis/endometritis (MET), and cystic ovarian disease (CYS) and their involvement in the selection programme of Holstein cattle. A procedure for the estimation of genetic parameters and genomic breeding values was proposed. The dataset included farmers' records of 127 000 lactations from 109 farms for RP, 185 000 lactations from 131 farms for MET and 115 000 lactations from 87 farms for CYS. The lactational incidence was 6.9% (RP), 12.8% (MET) and 9.2% (CYS). The single-trait animal model equation included fixed effects of herd-year-season of calving and parity of the cow, random direct additive genetic effect, random permanent environmental effect and residual effect. The single-step genomic prediction (ssGBLUP) was based on more than 50 000 SNP-genotyped individuals. Heritability for all traits did not exceed 2%. Favourable correlations were found between reproductive disorders resistance, fertility and longevity traits. The correlation between CYS and milk protein yield indicated a genetic predisposition to cystic ovarian disease in high-producing cows. All significant correlations between health traits (clinical mastitis, claw and feet disorders, reproductive disorders) were positive, indicating that the selection for improvement of one health trait would induce a favourable selection response in the others. Keywords: dairy cattle, retained placenta, uterine inflammation, ovarian cysts, genetic parameters, single-step genomic prediction Journal: Czech Journal of Animal Science Pages: 433-442 Volume: 68 Issue: 11 Year: 2023 DOI: 10.17221/86/2023-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/86/2023-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202311-0002.txt Handle: RePEc:caa:jnlcjs:v:68:y:2023:i:11:id:86-2023-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Ivan Bahelka Author-Workplace-Name: Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Roman Stupka Author-Workplace-Name: Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Jaroslav Čítek Author-Workplace-Name: Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Michal Šprysl Author-Workplace-Name: Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Title: The occurrence and risk assessment of bisphenol A and its analogues - bisphenol B, F, S, Z and AF in the urine of lactating sows Abstract: Bisphenols (BP) are pollutants that are globally and widely distributed and adversely affect the health of humans. However, knowledge of their presence in animals, especially farm animals such as pigs, remains limited. In this study, the incidence of bisphenol A (BPA) and its five analogues - bisphenol B (BPB), bisphenol F (BPF), bisphenol S (BPS), bisphenol Z (BPZ) and bisphenol AF (BPAF) - was monitored in lactating sows on an actual pig farm. The urine samples of 27 sows were collected twice on the 3rd day after parturition, and stored at -20 °C until analysis using MDGC/MS. None of the lactating sows produced bisphenol-free urine. In contrast, the urine of all sows contained at least two bisphenols (eight females), most often five (10 females) or all six bisphenols (five females). The average concentrations of bisphenols in urine were in the order of BPB > BPA and BPS > BPZ > BPAF > BPF. The most frequently detected bisphenol was BPB (96.0% of samples) followed by BPA (89.0%), BPAF (78.0%), BPS (63.0%), BPF (52.0%) and BPZ (37.0%). Additionally, the proportion of BPB in the total bisphenol concentration in urine samples was the highest (28.74 to 93.85%) while that of BPF was the lowest (2.33 to 16.70%). Estimated daily intakes of bisphenols as well as hazard quotients were much lower than risk thresholds established for the human population, indicating safe doses for the health status of lactating sows. However, these findings are limited as the mechanism of BPA analogue' activity is still unclear, and the long-term effects of small doses of bisphenols and the potential harmful impact of BP mixtures are unknown. Knowledge of the occurrence of bisphenols in pig farming may contribute to the elimination of BPA and its analogues from this sector, which is crucial for the safety of animal products as well as the welfare of pigs. Keywords: endocrine disruptor, pig, urine, estimated daily intake Journal: Czech Journal of Animal Science Pages: 451-459 Volume: 68 Issue: 11 Year: 2023 DOI: 10.17221/90/2023-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/90/2023-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202311-0003.txt Handle: RePEc:caa:jnlcjs:v:68:y:2023:i:11:id:90-2023-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Dae-Jin Kwon Author-Workplace-Name: Department of Animal Biotechnology, Jeonbuk National University, Jeonju, Republic of Korea Author-Name: Kyung-Do Park Author-Workplace-Name: Department of Animal Biotechnology, Jeonbuk National University, Jeonju, Republic of Korea Author-Name: Lee HakKyo Author-Workplace-Name: Department of Animal Biotechnology, Jeonbuk National University, Jeonju, Republic of Korea Author-Workplace-Name: International Agricultural Development and Cooperation Center, Jeonbuk National University, Jeonju, Republic of Korea Author-Name: Jae-Don Oh Author-Workplace-Name: Department of Animal Biotechnology, Jeonbuk National University, Jeonju, Republic of Korea Title: Lipid-rich bovine serum albumin is beneficial for the early development of bovine embryos, but induces lipid droplet formation in the blastocyst Abstract: Lipid-rich bovine serum albumin (LR-BSA) has been reported to increase the survival rate of blastocysts after freezing and thawing. However studies on the early development of in vitro fertilised (IVF) embryos are still insufficient in cattle. This study investigated the blastocyst productivity of in vitro culture (IVC) medium (synthetic oviductal fluid medium with amino acids, mSOFaa) supplemented with 5% fetal bovine serum (FBS, Control) or LR-BSA (1, 2.5, 5 mg/ml dosages, respectively) and the qualitative characteristics of produced blastocysts. Cleavage rates on day 2 were similar between all groups. Significant differences were obtained in the blastocyst rate in the 2.5LR-BSA group compared with the control (23.5% vs 35.7%, P < 0.05). Hatching rates were significantly higher in all treatment groups than in control (P < 0.05). The cell number in blastocysts did not differ among groups, but the apoptotic index in the 5.0LR-BSA group was higher than the control (P < 0.05). The expression of embryo quality-related markers was affected. Octamer-binding transcription factor 4 (Oct4), placenta associated 8 (Plac8), and sex-determining region Y-box 2 (Sox2) genes expression in the 2.5LR-BSA group were significantly up-regulated compared to the control (P < 0.05). Cytoplasmic lipid contents and the lipid droplet formation-related gene, sterol regulatory element binding transcription factor 1 (Srebf1), were significantly increased in the 5.0LR-BSA group (P < 0.05). Therefore, the LR-BSA supplementation (2.5 mg/ml) to mSOFaa medium could contribute to the early development of bovine IVF embryos and the qualitative improvement of the resulting blastocysts. Keywords: cattle, apoptosis, embryo quality, in vitro fertilised embryo Journal: Czech Journal of Animal Science Pages: 443-450 Volume: 68 Issue: 11 Year: 2023 DOI: 10.17221/107/2023-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/107/2023-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202311-0004.txt Handle: RePEc:caa:jnlcjs:v:68:y:2023:i:11:id:107-2023-CJAS