Template-Type: ReDIF-Article 1.0 Author-Name: Bin Song Author-Workplace-Name: College of Animal Science, Wenzhou Vocational College of Science & Technology, Wenzhou, PR China Author-Name: Shengwei Di Author-Workplace-Name: College of Animal Science and Technology, Northeast Agricultural University, Harbin, PR China Author-Name: Yan Zhao Author-Workplace-Name: College of Animal Science, Wenzhou Vocational College of Science & Technology, Wenzhou, PR China Author-Name: Ziwei Li Author-Workplace-Name: College of Animal Science, Wenzhou Vocational College of Science & Technology, Wenzhou, PR China Author-Name: Junjie Jin Author-Workplace-Name: College of Animal Science, Wenzhou Vocational College of Science & Technology, Wenzhou, PR China Author-Name: Fengxiang Hou Author-Workplace-Name: College of Animal Science, Wenzhou Vocational College of Science & Technology, Wenzhou, PR China Author-Name: Yawen Zhang Author-Workplace-Name: College of Animal Science, Wenzhou Vocational College of Science & Technology, Wenzhou, PR China Author-Name: Hongxi Xu Author-Workplace-Name: College of Animal Science, Wenzhou Vocational College of Science & Technology, Wenzhou, PR China Title: Effects of plant sterol microcapsules on growth performance and serum biochemical indicators in pigs Abstract: To better explore the effects of adding phytosterol (PS) microcapsules to feed on pig growth performance, nutrient apparent digestibility, and serum biochemical indicators, 200 healthy ternary hybrid Duroc × Landrace × Yorkshire piglets with an average initial weight of 7.53 ± 0.57 kg) were used as experimental subjects, and randomly divided into five groups with four replicates in each group. The control group of pigs was fed the basic diet, while the experimental group received diets supplemented with different PS levels. They were divided into experimental groups 1 to 4 (100 mg/kg to 5 502 mg/kg) according to different dosage added components. The duration of the experiment was 54 days. The results showed that the total triglyceride (TG) index and diarrhoea status in the experimental group were significantly improved compared to the control group (P < 0.05). The increase in PS addition levels was more significant in the change of pig average daily gain (ADG) (P < 0.05), and the difference in average daily weight gain and feed conversion ratio between PS-400 and PS-550 was significant (P < 0.05). Compared with the control group, the experimental group showed significant differences in crude fibre (CF), ether extract (EE), dry matter (DM), organic matter (OM), average digestible energy (ADE), metabolizable energy, xylan, and calcium (P < 0.05), with a maximum increase of 7.02% in calcium content. The addition of PS can be effective in making the experimental and control groups show a significant difference in value changes in high-density cholesterol (HDL-C) and low-density cholesterol (LDL-C) indicators (P < 0.05), while no significant changes were revealed in the other indicators (P > 0.05). From the above, plant sterol microcapsules can effectively improve pig growth performance and nutrient apparent digestibility, and improve their blood lipid status. Keywords: plant sterols, pig, diarrhoea rate, nutrient digestibility, aspartate aminotransferase, alanine aminotransferase, high-density lipoprotein cholesterol Journal: Czech Journal of Animal Science Pages: 497-507 Volume: 68 Issue: 12 Year: 2023 DOI: 10.17221/64/2023-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/64/2023-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202312-0001.txt Handle: RePEc:caa:jnlcjs:v:68:y:2023:i:12:id:64-2023-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Momčilo Šaran Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia Author-Name: Ljuba Štrbac Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia Author-Name: Dobrila Janković Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia Author-Name: Mihajla Djan Author-Workplace-Name: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia Author-Name: Snežana Trivunović Author-Workplace-Name: Department of Animal Science, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia Author-Name: Minja Zorc Author-Workplace-Name: Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia Title: Genomic characterization of Serbian Holstein-Friesian cattle population Abstract: The use of genomic data makes it possible to examine genetic variability and calculate the genetic parameters of the population in an efficient and precise way. The aim of this research was to analyse linkage disequilibrium (LD), contemporary effective population size (Nec), haplotype block structure, minor allele frequency (MAF), observed (HO) and expected heterozygosity (HE), calculate the genomic relationship matrix and perform a principal component analysis (PCA) in the Serbian Holstein-Friesian cattle population using SNP data from the GGP Bovine 100K chip. After quality control (QC), 83 208 SNPs and 1 575 cows were retained for further analysis. LD on autosomes had an average value of ≥ 0.2 up to a distance of 50-60 kb (r2 = 0.211), while on BTX r2 ≥ 0.2 was represented at distances of 80-90 kb (r2 = 0.211). LD differed between chromosomes. The average HO for autosomes and X chromosome SNPs was 0.412 and 0.422, respectively. 81.30% of SNPs that passed QC had MAF > 0.2. The total number of haplotype blocks in the studied population was 15 642. On average, blocks contained 2.932 SNPs. The average block length was 32.657 kb and ranged from a minimum of 0.019 kb (BTA21 and BTA26) to a maximum of 999.562 kb (BTX). The estimated value of Nec in the this cattle population was 142. The results of PCA showed a significant variability of genotypes in the population, but there was no clear stratification of the population. The obtained results will serve as a basis for future genomic analyses such as the detection of QTLs for important economic traits and the implementation of genomic selection. Keywords: single nucleotide polymorphism, genetic diversity, genomic analysis, linkage disequilibrium, haplotype block Journal: Czech Journal of Animal Science Pages: 486-496 Volume: 68 Issue: 12 Year: 2023 DOI: 10.17221/89/2023-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/89/2023-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202312-0002.txt Handle: RePEc:caa:jnlcjs:v:68:y:2023:i:12:id:89-2023-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Ning Liu Author-Workplace-Name: Department of Animal Science, Henan University of Science and Technology, Luoyang, PR China Author-Name: Yien Lin Author-Workplace-Name: Department of Animal Science, Henan University of Science and Technology, Luoyang, PR China Author-Name: Shuaibao Wang Author-Workplace-Name: Center of Animal Disease Control, Municipal Bureau of Agriculture and Rural Affair, Sanmenxia, PR China Author-Name: Jianping Wang Author-Workplace-Name: Department of Animal Science, Henan University of Science and Technology, Luoyang, PR China Title: Effect of catalase on the growth performance, antioxidation, and microbial metabolism of weaned rabbits Abstract: The present study aimed to investigate the effect of catalase (CAT) on the growth performance, antioxidation, and microbial metabolism of weaned rabbits. Dietary treatments consisted of control and CAT supplementation at 100 (T1), 150 (T2), and 200 IU/kg of diet (T3). A total of 240 weaned rabbits were randomly assigned to 4 groups with 6 replicates of 10 rabbits each. The feeding trial lasted for 28 days. Results showed that T2 and T3 increased (P < 0.05) body weight gain and gain/feed ratio, and decreased (P < 0.05) diarrhoea rate, compared to the control. Also, serum antioxidative parameters and oxidative stress products were beneficially regulated (P < 0.05) by the dietary CAT administration. Faecal microbiota including Bacteroidetes, Prevotella, and Bifidobacterium in T2 or T3 was increased (P < 0.05). Dietary CAT with changed microbiota further influenced the metabolites from carbohydrates and proteins, evidenced by increased lactic acid, acetate, branched-chain fatty acids, and short-chain fatty acids, and decreased valerate, isovalerate, methylamine, tryptamine, putrescine, cadaverine, spermidine, and total amines. It is concluded that CAT can be used to improve growth performance by beneficially regulating the antioxidation, microbiota, and metabolites in weaned rabbits. Keywords: amine, gut microbe, short-chain fatty acid, oxidant injury product Journal: Czech Journal of Animal Science Pages: 508-515 Volume: 68 Issue: 12 Year: 2023 DOI: 10.17221/133/2023-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/133/2023-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202312-0003.txt Handle: RePEc:caa:jnlcjs:v:68:y:2023:i:12:id:133-2023-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Filipp Georgijevič Savvulidi Author-Workplace-Name: Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic Author-Name: Martin Ptáček Author-Workplace-Name: Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic Author-Name: Szabolcs Nagy Author-Workplace-Name: Institute of Animal Sciences, Hungarian University of Agriculture and Life Sciences, Georgikon Campus, Keszthely, Hungary Author-Name: Luděk Stádník Author-Workplace-Name: Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic Title: Meta-analysis of fluorochrome concentrations to assess bull sperm quality by flow cytometry in 2021-2023 Abstract: Flow cytometry (FC) is a very powerful method for the assessment of spermatozoa quality in multiple livestock species, including bulls. Nowadays, many flow cytometric tests are available for quality detection of bull sperm. These tests are based on fluorochromes (fluorescent tags or dyes) and the use of correct concentrations of fluorochromes is an essential aspect of the successful optimization of experimental methodology. Modern flow cytometers are much more affordable, such that spermatologists are now able to use FC methodology (and even image cytometry) in their studies. The present review aims to give a summary of current flow cytometric methodologies used to assess bull sperm quality by flow cytometry. Namely, we give here the concentrations of fluorochromes used in the studies related to bull sperm quality evaluation by flow cytometry published in the last three years (from 2021 to 2023). Importantly, in the present review, the concentration of fluorochromes is unified and presented using comparable molarity units. Furthermore, the rationale of each flow cytometric methodology for particular fluorescent tests, and the citation for the corresponding original methodology, are given. This review will help spermatologists shorten the time needed for the optimization of their flow cytometry methodology for the assessment of bull sperm quality. Keywords: DNA stability, plasma membrane integrity, acrosomal status, mitochondrial status, oxidative stress, intracellular calcium, analysis accuracy, bovine sperm Journal: Czech Journal of Animal Science Pages: 469-485 Volume: 68 Issue: 12 Year: 2023 DOI: 10.17221/152/2023-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/152/2023-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202312-0004.txt Handle: RePEc:caa:jnlcjs:v:68:y:2023:i:12:id:152-2023-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Editorial Department Title: Index Contents CJAS volume 68 Journal: Czech Journal of Animal Science Pages: I-VIII Volume: 68 Issue: 12 Year: 2023 File-URL: http://cjas.agriculturejournals.cz/artkey/cjs-202312-0005_index-contents-cjas-volume-68.php File-Format: text/html Handle: RePEc:caa:jnlcjs:v:68:y:2023:i:12:id:cjs-202312-0005