Template-Type: ReDIF-Article 1.0 Author-Name: Oto Hanuš Author-Workplace-Name: Dairy Research Institute Ltd., Prague, Czech Republic Author-Name: Eva Samková Author-Workplace-Name: Department of Food Biotechnologies and Agricultural Products Quality, Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic Author-Name: Jindřich Čítek Author-Workplace-Name: Department of Genetics and Agricultural Biotechnology, Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic Author-Name: Hana Nejeschlebová Author-Workplace-Name: Dairy Research Institute Ltd., Prague, Czech Republic Author-Workplace-Name: Department of Food Biotechnologies and Agricultural Products Quality, Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic Author-Name: Eva Dadáková Author-Workplace-Name: Department of Applied Chemistry, Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic Author-Name: Lucie Hasoňová Author-Workplace-Name: Department of Food Biotechnologies and Agricultural Products Quality, Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic Author-Name: Michael Rost Author-Workplace-Name: Department of Genetics and Agricultural Biotechnology, Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic Author-Name: Irena Němečková Author-Workplace-Name: Dairy Research Institute Ltd., Prague, Czech Republic Author-Name: Karolína Reindl Author-Workplace-Name: Department of Food Biotechnologies and Agricultural Products Quality, Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic Author-Name: Markéta Borková Author-Workplace-Name: Dairy Research Institute Ltd., Prague, Czech Republic Author-Name: Ondřej Elich Author-Workplace-Name: Dairy Research Institute Ltd., Prague, Czech Republic Title: The stability of fatty acids in yoghurts produced from bulk milk samples intentionally selected according to dairy production systems Abstract: The fatty acid (FA) composition of milk fat can be positively influenced by the modification of dairy farming and the possible selection of raw material for processing. The question arises as to whether the benefits of a better FA composition will be maintained even after processing dairy products. Thus, the aim was to obtain a more favourable FA composition by a targeted selection of cow's milk (grazing vs stabled herds) and then compare FAs of milk and fermented product (yoghurt). Bulk tank milk of grazing herds had significantly better FA composition than milk of stabled herds (for example, C16:0 28.07% vs 32.27%, P < 0.001; C18:3n-3 0.83% vs 0.41%, P < 0.001; C18:2 cis-9, trans-11 (conjugated linoleic acid; CLA) 1.02% vs 0.41%, P < 0.01). The differences between the FAs in milk and yoghurt samples were negligible (in relative values from 0.04% to 5.21%). The correlations between milk and yoghurt for nutritionally important FAs were high, from 0.925 0 (C18:2n-6) to 0.998 8 (CLA; both P < 0.001). The minimal effect of milk fermentation on the original FA composition of milk fat was found. In conclusion, systematic selection of raw cow's milk or modification of farming conditions can also provide a nutritionally desirable composition of final dairy products. Keywords: dairy cow, fermentation, grazing herd, milk fat, stabled herd Journal: Czech Journal of Animal Science Pages: 303-316 Volume: 69 Issue: 8 Year: 2024 DOI: 10.17221/84/2024-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/84/2024-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202408-0001.txt Handle: RePEc:caa:jnlcjs:v:69:y:2024:i:8:id:84-2024-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: János Posta Author-Workplace-Name: Department of Animal Husbandry, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary Author-Name: Noémi Anikó Szabó Author-Workplace-Name: Department of Animal Husbandry, Institute of Animal Science, Biotechnology and Nature Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary Author-Name: Róbert Juráskó Author-Workplace-Name: Institute of Animal Sciences, Hungarian University of Agriculture and Life Sciences (MATE), Kaposvár, Hungary Title: Pedigree-based genetic diversity of Debrecen White rabbit Abstract: This research was carried out to evaluate the pedigree data of the Debrecen White rabbit breed. Pedigree information was supplied by the Debrecen White Rabbit Breeder Association. The final dataset contained all available information on animals registered by the breeding association up to 2023. The reference population was the active breeding stock in 2023. The estimated complete generation equivalent was 7.8, while the mean of maximum generations was 16.29 for the present stock. All individuals within the current population were inbred, with a 5.37% mean inbreeding coefficient. The division of the inbreeding coefficient showed that homozygosity is increasing in the current population. The genetic conservation index (GCI) was higher than 30 for 10.11% for the total population, and was above 38.69% for the current population. Keywords: genetic variability, inbreeding, pedigree analysis Journal: Czech Journal of Animal Science Pages: 317-322 Volume: 69 Issue: 8 Year: 2024 DOI: 10.17221/116/2024-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/116/2024-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202408-0002.txt Handle: RePEc:caa:jnlcjs:v:69:y:2024:i:8:id:116-2024-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Zhiming Zhu Author-Workplace-Name: Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, P. R. China Author-Workplace-Name: Fujian Key Laboratory of Animal Genetics and Breeding, Fuzhou, P. R. China Author-Name: Linli Zhang Author-Workplace-Name: Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, P. R. China Author-Workplace-Name: Fujian Key Laboratory of Animal Genetics and Breeding, Fuzhou, P. R. China Author-Name: Qingwu Xin Author-Workplace-Name: Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, P. R. China Author-Workplace-Name: Fujian Key Laboratory of Animal Genetics and Breeding, Fuzhou, P. R. China Author-Name: Li Li Author-Workplace-Name: Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, P. R. China Author-Workplace-Name: Fujian Key Laboratory of Animal Genetics and Breeding, Fuzhou, P. R. China Author-Name: Zhongwei Miao Author-Workplace-Name: Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, P. R. China Author-Workplace-Name: Fujian Key Laboratory of Animal Genetics and Breeding, Fuzhou, P. R. China Author-Name: Qinlou Huang Author-Workplace-Name: Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, P. R. China Author-Workplace-Name: Fujian Key Laboratory of Animal Genetics and Breeding, Fuzhou, P. R. China Author-Name: Nenzhu Zheng Author-Workplace-Name: Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, P. R. China Author-Workplace-Name: Fujian Key Laboratory of Animal Genetics and Breeding, Fuzhou, P. R. China Title: Whole-genome sequencing revealed the population structure of Fujian chicken breeds Abstract: Indigenous chicken breeds from Fujian Province, China, exhibit unique and distinctive phenotypic characteristics, including morphology, behaviour, integumentary pigmentation, feather colouration, and production performance. However, the underlying genetic basis governing these specific traits remains unclear. This study aimed to elucidate the population structure of six Fujian local chicken breeds [Bairong chickens (BRCs), Jinhu chickens (JHCs), Minqing chickens (MQCs), Dehua chickens (DHCs), Xiangdong chickens (XDCs), and Hetian chickens (HTCs)] in terms of their genetic resources within the province. The whole-genome resequencing revealed 8 678 612 single nucleotide polymorphisms (SNPs) in a cohort of 36 chickens. The results revealed a partial mismatch between the clustering patterns and geographic distributions of the six populations, with BRC displaying a noticeable genetic divergence from other chickens. Moreover, the genetic distance between XDC and HTC in Longyan County was relatively close and converged with JHC, MQC, and DHC into a subgroup. These findings provide a comprehensive understanding of the intrinsic characteristics and utilisation prospects of chicken germplasm resources in Fujian Province. Keywords: indigenous chicken, genetic structure, genomic resequencing, germplasm resource Journal: Czech Journal of Animal Science Pages: 323-330 Volume: 69 Issue: 8 Year: 2024 DOI: 10.17221/91/2023-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/91/2023-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202408-0003.txt Handle: RePEc:caa:jnlcjs:v:69:y:2024:i:8:id:91-2023-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Ege Güngör Author-Workplace-Name: Institute of Graduate Studies in Science and Engineering, Istanbul University, Istanbul, Türkiye Author-Name: Aygül Ekici Author-Workplace-Name: Department of Aquaculture, Faculty of Aquatic Sciences, Istanbul University, Istanbul, Türkiye Author-Name: Merve Erçin Author-Workplace-Name: Department Of Molecular Biology, Faculty Of Science, Istanbul University, Istanbul, Türkiye Author-Name: Selda Oktayoğlu Author-Workplace-Name: Department Of Molecular Biology, Faculty Of Science, Istanbul University, Istanbul, Türkiye Author-Name: Taiju Saito Author-Workplace-Name: Institution for Collaborative Relations, South Ehime Fisheries Research Center, Ehime University, Ehime, Japan Author-Name: İlker Keskin Author-Workplace-Name: Institute of Graduate Studies in Science and Engineering, Istanbul University, Istanbul, Türkiye Title: Cryopreservation of early-stage male Black Sea trout (Salmo trutta labrax) germ cells and comparison of the whole tissue and enzymatically isolated cells Abstract: The high plasticity, or the ability to differentiate into various cell types, and capacity to become both gametes of early-stage germ cells (eGCs) allows them to be used for the long-term storage and recovery of genetic information. In this study, species-specific protocols (isolation period, enzymatic separation solution, incubation period, appropriate cryoprotectant, and cryopreservation protocol) were established for the isolation and long-term preservation of Black Sea trout eGCs. In addition, the difference between the application of cryopreservation to the whole gonad tissue and to the enzymatically separated cells has been shown in terms of viability and the number of cells obtained. According to the cell count made from the histological sections of the testicular tissue throughout the year, the period with the highest concentration of eGCs in the tissue is between May and June. To identify the optimal enzymatic dissociation solution, we subjected testicular tissues to digestion using various combinations of trypsin (T) and collagenase (C) (0.3-0.5% T and 0.1-0.3% C). Our findings revealed that the 0.3% C provided the highest yield of viable cells (90.9%). We cryopreserved the enzymatically dissociated cells (EDCs) using six different cryoprotectants (Dimethyl sulfoxide, Glycerol, and CryoSOfree™, in both L-15 and phosphate-buffered saline - PBS mediums). Additionally, whole tissue exclusively with CryoSOfree™. After storing the samples at -152 °C for two years, the group of EDCs cryopreserved in L15+Glycerol exhibited the highest viability at 93.3%. Meanwhile, the whole tissue group yielded the greatest number of cells per ml after thawing, with a count of 33 210 000 cells. When considering both the viability (91.11%) and the number of cells obtained (33 210 000 cells/ml), the whole tissue cryopreservation group outperformed all the other methods. Keywords: enzymatic dissociation, long-term storage, Salmonidae, spermatogonia Journal: Czech Journal of Animal Science Pages: 331-343 Volume: 69 Issue: 8 Year: 2024 DOI: 10.17221/71/2024-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/71/2024-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202408-0004.txt Handle: RePEc:caa:jnlcjs:v:69:y:2024:i:8:id:71-2024-CJAS