Template-Type: ReDIF-Article 1.0 Author-Name: Radka Malinova Author-Workplace-Name: Department of Animal Sciences, Agricultural University, Plovdiv, Bulgaria Author-Name: Pencho Malinov Author-Workplace-Name: Department of Computer Systems, University of Plovdiv, Plovdiv, Bulgaria Author-Name: Evgeni Valchev Author-Workplace-Name: Department of Computer Systems, University of Plovdiv, Plovdiv, Bulgaria Author-Name: Todorka Glushkova Author-Workplace-Name: Department of Computer Technology, University of Plovdiv, Plovdiv, Bulgaria Title: Approach to creating an intelligent system for free-range livestock farming Abstract: The development of intelligent systems for the tracking of free-range livestock is a challenge to both information and communication technology (ICT) scientists and those in the animal sciences. Cyber-physical systems make it possible to track and control processes involving intelligent objects from the physical and virtual worlds. In the case of free-range grazing, it is necessary to manage processes in two domains ‒ that of the intelligent pasture management and that of the animals. Due to the differences in the conditions of different types of pastures - plain or high land and the characteristics of the cattle breeds, ready-made models cannot be used, but it is necessary to build a specific multi-aspect model for the behaviour and life cycle of cows. Our team organised their research on cows from two different breeds (Rhodope Shorthorn Cattle and Bulgarian Rhodope Cattle) raised in similar technologies, grazed on two different types of pasture. The aim of the study is to develop a comprehensive model for determining cattle behavioural activities on pastures using sensor groups, by incorporating physical observations and appropriate statistical models. Keywords: Cyber-Physical Systems (CPS), intelligent agriculture, intelligent livestock, intelligent pasture Journal: Czech Journal of Animal Science Pages: 389-399 Volume: 69 Issue: 10 Year: 2024 DOI: 10.17221/90/2024-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/90/2024-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202410-0001.txt Handle: RePEc:caa:jnlcjs:v:69:y:2024:i:10:id:90-2024-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Michal Ďuračka Author-Workplace-Name: AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Nitra, Slovakia Author-Name: Filip Benko Author-Workplace-Name: Institute of Biotechnology, Slovak University of Agriculture in Nitra, Nitra, Slovakia Author-Name: Miroslava Kačániová Author-Workplace-Name: Institute of Horticulture, Slovak University of Agriculture in Nitra, Nitra, Slovakia Author-Workplace-Name: School of Medical & Health Sciences, University of Economics and Human Sciences in Warsaw, Warszaw, Poland Author-Name: Eva Tvrdá Author-Workplace-Name: Institute of Biotechnology, Slovak University of Agriculture in Nitra, Nitra, Slovakia Title: Comparative study: Efficacy of egg-yolk vs soy lecithin-based diluent in preservation of chilled bovine semen - Bacteriology and sperm quality Abstract: This study compared the efficacy of the egg yolk (EY) and soy lecithin (SL) semen extender during 72-h storage at 4 °C in the presence/absence of streptomycin, penicillin, lincomycin, and spectinomycin. Bull semen samples (n = 12) were obtained and stored in either ANDROMED® (SL extender) or TRILADYL CSS® (EY extender). After 24, 48, and 72 h of storage at 4 °C, bacterial colonies (CFU - colony forming units) were grown and counted on tryptic soy and blood agar, and the bacterial isolates were identified using the MALDI-TOF MS Biotyper. Sperm motility, mitochondrial membrane potential (MMP), membrane and acrosome integrity, sperm DNA fragmentation, and reactive oxygen species (ROS) production were analysed. The results showed that the type of semen diluent could be crucial for the efficacy of used antibiotics, since significantly decreased bacterial occurrence following 48 h or no bacterial growth after 72 h was recorded in the SL-based groups. Even the SL medium itself did not favour bacterial growth, as significantly decreased bacterial load was observed after 48 h (P < 0.01) and 72 h (P <0.000 1). In contrast, the bacterial load in the EY medium without antibiotics significantly increased (P < 0.05). In contrast, an improved ability to preserve sperm quality parameters was observed in EY-extended semen. In particular, sperm motility was significantly higher (P < 0.000 1 and P < 0.001) after 24 h in each EY-based group in comparison to the control (Ctrl), whereas among the SL-based groups, only the spectinomycin-supplemented group presented with a significant motility improvement (P < 0.01) when compared to the Ctrl. Although the EY semen extender provided enhanced sperm quality preservation during the 72-h storage, in the future, the SL medium composition should be improved to match the sperm preservation ability of the EY medium while maintaining its microbial safety. Keywords: bacteria, bull semen extender, cooled spermatozoa, liquid semen storage Journal: Czech Journal of Animal Science Pages: 400-409 Volume: 69 Issue: 10 Year: 2024 DOI: 10.17221/138/2024-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/138/2024-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202410-0002.txt Handle: RePEc:caa:jnlcjs:v:69:y:2024:i:10:id:138-2024-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Yuanxiao Li Author-Workplace-Name: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, P. R. China Author-Name: Yan Yu Author-Workplace-Name: College of Biological and Pharmaceutical Engineering, West Anhui University, Liuan, P. R. China Author-Name: Feiyan Zhao Author-Workplace-Name: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, P. R. China Author-Name: Zihan Zhao Author-Workplace-Name: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, P. R. China Author-Name: Mengying Dou Author-Workplace-Name: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, P. R. China Author-Name: Zhijun Cao Author-Workplace-Name: College of Animal Science and Technology, China Agricultural University, Beijing, P. R. China Author-Name: Wang Li Author-Workplace-Name: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, P. R. China Author-Name: Ke Ding Author-Workplace-Name: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, P. R. China Author-Name: Cai Zhang Author-Workplace-Name: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, P. R. China Title: Glutamine protects cow's ruminal epithelial cells from acid-induced injury in vitro Abstract: This study was conducted to investigate the effects and mechanisms of glutamine (Gln) on the repair of acid-induced injury in dairy cow ruminal epithelial cells (RECs) in vitro. Dairy cow RECs were cultured in a medium with pH of 5.5 for 3 h and subsequently treated with various concentrations of Gln (4, 8, 12, 32 mmol/l) for 12 h. The messenger ribonucleic acid (mRNA) expression levels of occludin (OCLN), claudin 1 (CLDN1), toll-like receptor 2 (TLR2), toll-like receptor 4 (TLR4) and genes for inflammatory factors were measured using reverse transcription-quantitative real-time PCR (RT-qPCR). The results showed that cellular activity and OCLN expression were significantly highest at 8 mmol/l Gln (P < 0.05). CLDN1 expression was significantly higher at 4 mmol/l Gln compared to the other groups (P < 0.05). The relative expression levels of tumour necrosis factor (TNF), interleukin 1B (IL1B), C-X-C motif chemokine ligand 8 (CXCL8), TLR2 and TLR4 in the acid treatment group were significantly higher than those in the control group (P < 0.05), but they were lower in the Gln-treated groups than in the acid treatment group (P < 0.05). These findings demonstrate that Gln promotes the proliferation of RECs, enhances the expression of epithelial cell junction proteins, and inhibits the expression of inflammatory factors and surface receptors. In conclusion, Gln shows a potential for repairing acid-induced injury in RECs. Keywords: bovine, cytokine regulation, gene regulation, RT-qPCR, tight junction integrity Journal: Czech Journal of Animal Science Pages: 410-418 Volume: 69 Issue: 10 Year: 2024 DOI: 10.17221/170/2023-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/170/2023-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202410-0003.txt Handle: RePEc:caa:jnlcjs:v:69:y:2024:i:10:id:170-2023-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Katalin Szendrő Author-Workplace-Name: Institute of Agricultural and Food Economics, Kaposvár Campus, Hungarian University of Agriculture and Life Sciences, Kaposvár, Hungary Author-Name: Antonella Dalle Zotte Author-Workplace-Name: Department of Animal Medicine, Production and Health, University of Padova, Padova, Italy Author-Name: Nikoletta Fülöp Author-Workplace-Name: Institute of Agricultural and Food Economics, Kaposvár Campus, Hungarian University of Agriculture and Life Sciences, Kaposvár, Hungary Author-Name: Mónika Zita Nagy Author-Workplace-Name: Institute of Agricultural and Food Economics, Kaposvár Campus, Hungarian University of Agriculture and Life Sciences, Kaposvár, Hungary Title: A survey on rabbit meat perception and consumption in seven countries Abstract: The aim of the study was to examine the rabbit consumption habits and opinions of people living in countries that regularly consume rabbit meat. Snowball sampling of data collection was used, a total of 1 860 error-free questionnaires were evaluated. Most of the completed questionnaires came from Hungary, Italy, Spain, China, Poland, France and Mexico. It is to note that 21.3% of respondents have not yet consumed rabbit meat: China (32.1%), Hungary (24.7%), Poland (22.4%), and France (4.8%). The main reasons for not consuming rabbit meat were emotional reasons (22.3%), it does not fit their dietary habits (15.7%). There are 28.7% of respondents who consume rabbit meat once or twice a year, 18.6% less often and 21.9% monthly. It was most often (weekly and monthly) consumed in Spain (25.3% and 36.8%), in Italy (15.3% and 36.2%), in France (1.6% and 38.7%, respectively). Men, aged 40 to 59, secondary school graduates, and those having a higher income eat rabbit meat more often than other groups. The highest scores (near to 4.5 on a 1-5 scale) were given for the health aspects of rabbit meat, such as high protein, omega-3 fatty acids and vitamins, and low fat and cholesterol contents. In order to encourage the consumption of rabbit meat, different marketing campaigns should be carried out in each country, depending on the standard of living, production intensity (large-scale or small-scale) and place of purchase (supermarket or local market). It is paramount to make the younger generation aware of the excellent and health-protective properties of rabbit meat. Keywords: attitude, consumer, food, opinion, Oryctolagus cuniculus Journal: Czech Journal of Animal Science Pages: 419-429 Volume: 69 Issue: 10 Year: 2024 DOI: 10.17221/123/2024-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/123/2024-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202410-0004.txt Handle: RePEc:caa:jnlcjs:v:69:y:2024:i:10:id:123-2024-CJAS