Template-Type: ReDIF-Article 1.0 Author-Name: Lucia Olexiková Author-Workplace-Name: Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic Author-Name: Linda Dujíčková Author-Workplace-Name: Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic Author-Name: Jaromír Vašíček Author-Workplace-Name: Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic Author-Workplace-Name: Institute of Biotechnology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic Author-Name: Andrej Baláži Author-Workplace-Name: Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic Author-Name: Andrea Svoradová Author-Workplace-Name: Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic Author-Name: Lenka Kuželová Author-Workplace-Name: Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic Author-Workplace-Name: AgroBioTech Research Centre, Slovak University of Agriculture, Nitra, Slovak Republicc Author-Name: Alexander Makarevich Author-Workplace-Name: Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic Author-Name: Peter Ivanič Author-Workplace-Name: Slovak Biological Services, Nitra, Slovak Republic Author-Name: Peter Chrenek Author-Workplace-Name: Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic Author-Workplace-Name: Institute of Biotechnology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic Title: Quality and in vitro fertilising ability of cryostored Pinzgau bull insemination doses Abstract: Applicability of cryopreserved insemination doses is dependent on the quality of the semen after thawing. The aim of the study was to check the options of new markers for the assessment of sperm quality to predict their fertilisation ability. In five bulls of the Pinzgau breed, the sperm fertilising ability was determined using the in vitro fertilisation. Bulls were divided according to the achieved blastocyst rate: bulls with good fertilising ability (NOB - 38.6%, KAZ - 28.9%, GAL - 29.3%) and bulls with low fertilising ability (LOH - 19.4%, NUS - 22.1%). In addition to the sperm motility and morphology, we monitored common physiological sperm characteristics: viability, apoptosis, acrosomal status, capacitation, mitochondrial activity and generation of reactive oxygen species using the flow cytometry procedure. Novel fertility-related biomarkers, such as ubiquitination, overexpression of MKRN1, SPTRX-3 and PAWP proteins or histone modification (H3K4me2), were also analysed by flow cytometry. From all monitored parameters, more proper characteristics of impaired in vitro fertilising ability proved to be high incidence of apoptotic markers (YO-PRO, Caspase 3/7) and higher counts of morphologically abnormal spermatozoa. Although the in vitro fertilisation (IVF) test can be an advantageous method for evaluating the sperm fertilising ability, there are still differences between the in vivo and in vitro fertilisation processes, which must be considered for example, when evaluating the sperm capacitation status. Keywords: biomarker, bovine, fertilisation, flow cytometry, sperm Journal: Czech Journal of Animal Science Pages: 431-440 Volume: 69 Issue: 11 Year: 2024 DOI: 10.17221/142/2024-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/142/2024-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202411-0001.txt Handle: RePEc:caa:jnlcjs:v:69:y:2024:i:11:id:142-2024-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Farwa Shakeel Author-Workplace-Name: Faculty of Animal and Agricultural Science, Dipenogoro University, Semarang, Indonesia Author-Name: Yeni Widiawati Author-Workplace-Name: Research Centre for Animal Husbandry, National Research and Innovation Agency, Bogor, Indonesia Author-Name: Sharly Asmairicen Author-Workplace-Name: Research Centre for Animal Husbandry, National Research and Innovation Agency, Bogor, Indonesia Author-Name: Agung Purnomoadi Author-Workplace-Name: Faculty of Animal and Agricultural Science, Dipenogoro University, Semarang, Indonesia Author-Name: Agustin Herliatika Author-Workplace-Name: Research Centre for Animal Husbandry, National Research and Innovation Agency, Bogor, Indonesia Author-Name: Yenny Nur Anggraeny Author-Workplace-Name: Research Centre for Animal Husbandry, National Research and Innovation Agency, Bogor, Indonesia Author-Name: Setiasih Setiasih Author-Workplace-Name: Research Centre for Animal Husbandry, National Research and Innovation Agency, Bogor, Indonesia Author-Name: Muhammad Rizwan Author-Workplace-Name: Faculty of Animal and Agricultural Science, Dipenogoro University, Semarang, Indonesia Author-Name: Ruslan Abdul Gopar Author-Workplace-Name: Research Centre for Animal Husbandry, National Research and Innovation Agency, Bogor, Indonesia Author-Name: Antonius Antonius Author-Workplace-Name: Research Centre for Animal Husbandry, National Research and Innovation Agency, Bogor, Indonesia Author-Name: Igit Atang Sunante Author-Workplace-Name: Research Centre for Horticulture, National Research and Innovation Agency, Bogor Indonesia Author-Name: Idris Idris Author-Workplace-Name: Research Centre for Horticulture, National Research and Innovation Agency, Bogor Indonesia Author-Name: Susi Riyanti Author-Workplace-Name: Directorate of Laboratory Management, Research Facilities, and Science and Technology Park, National Research and Innovation Agency, Jakarta, Indonesia Author-Name: Winwin Widaringsih Author-Workplace-Name: Research Centre for Animal Husbandry, National Research and Innovation Agency, Bogor, Indonesia Author-Name: Faizan Ahmad Shakir Author-Workplace-Name: Faculty of medicine, Dipenogoro University, Semarang, Indonesia Author-Name: Saad Irfan Author-Workplace-Name: Faculty of Animal and Agricultural Science, Dipenogoro University, Semarang, Indonesia Title: The effect of the dried Gracilaria spp. undergoing different drying methods on in vitro rumen fermentation Abstract: Seaweed has an important role in the mitigation of enteric methane (CH4) production by ruminant animals. The utilisation and its effectiveness in enteric CH4 reduction require a preservation process. The aim of the study was to investigate the effect of different drying processes on the effectiveness of seaweed Gracilaria spp. in reducing CH4 production assessed through an in vitro method. Three drying techniques, sun-drying, oven-drying, and freeze-drying, were applied to produce a dried product of Glacilaria spp. Rice straw basal diets combined with concentrate at the 70 : 30% were used to test the inclusion of 4% of three differently dried products of Gracilaria spp. compared to the basal diet without seaweed (control group). Measurements were conducted on in vitro total and CH4 gas production, nutrient degradability, ammonia (NH3) and VFA concentration, and microbial population. Results showed that the three dried products of Gracilaria spp. significantly reduced in vitro CH4 production compared to the control group (P ≤ 0.05). All drying techniques gave a similar effect on in vitro CH4 reduction, but they did not affect dry matter (DM) and organic matter (OM) degradability (P ≤ 0.05) and significantly reduced neutral detergent fiber (NDF) degradability (P ≤ 0.05) compared to the control group, with oven-dried treatments having the lowest NDF degradability among the treatments. It is concluded that the three different drying techniques had similar effects on enteric CH4 reduction. Keywords: enteric methane, methane production, mitigation, preservation, seaweed Journal: Czech Journal of Animal Science Pages: 441-449 Volume: 69 Issue: 11 Year: 2024 DOI: 10.17221/75/2024-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/75/2024-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202411-0002.txt Handle: RePEc:caa:jnlcjs:v:69:y:2024:i:11:id:75-2024-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Maria Claudia Puerta Author-Workplace-Name: Faculty of Veterinary Medicine and Animal Science, CES University, Medellín, Colombia Author-Name: Laura Henao-Salazar Author-Workplace-Name: Faculty of Veterinary Medicine and Animal Science, CES University, Medellín, Colombia Author-Name: Isabel Catalina Vélez Author-Workplace-Name: Faculty of Veterinary Medicine and Animal Science, CES University, Medellín, Colombia Author-Name: Sergio León Author-Workplace-Name: Faculty of Agricultural Science, University of La Salle Universidad Nacional de Colombia, Bogotá, Colombia Author-Name: Banjamín Rojano Author-Workplace-Name: Faculty of Science, the university Universidad Nacional de Colombia, Medellín, Colombia Author-Name: Giovanni Restrepo Author-Workplace-Name: Faculty of Agricultural Science, the university Universidad Nacional de Colombia, Medellín, Colombia Author-Name: Alexandra Úsuga Author-Workplace-Name: Biogenesis Research Group, Faculty of Agricultural Science, University of Antioquia, Medellín, Colombia Title: Dietary antioxidant supplementation improves the in vitro quality and antioxidant capacity of Colombian Creole stallion semen Abstract: Dietary antioxidant supplementation has become popular for managing male infertility because of its impact on the antioxidant capacity of semen. This study evaluates the effect of dietary antioxidant supplementation on the seminal characteristics of Colombian Creole horses. Semen from six Colombian Creole stallions was collected using an artificial vagina. The stallions were randomly allocated to a control group (CG) or a supplementation group (SG) that received a multi-antioxidant supplement. A repeated measures design, with a wash out period of two weeks between treatments, was used. Sperm motility and kinematics were assessed using a computer-assisted semen analysis system, membrane integrity (MI) and the acrosomal integrity (AI) of sperm were assessed using fluorescent microscopy, abnormal morphology (AM) was analysed using supravital staining, and the functionality of the sperm membrane was assessed using the hypoosmotic swelling (HOS) test. A fraction of each ejaculate sample was centrifuged to obtain seminal plasma (SP). The reactive oxygen species and the total antioxidant capacity (TAC) of the SP were measured via spectrofluorimetry. Linear models were fitted, and comparison of means was performed using Tukey's test. Dietary antioxidant supplementation increased sperm beat cross frequency. In addition, a higher AI was found for the SG. Supplementation also increased the TAC of the SP. Individual analysis revealed that dietary antioxidant supplementation increased the total and progressive motility of the semen of two horses in the SG; however, supplementation reduced these two parameters in two other individuals compared to the CG. It can be concluded that dietary antioxidant supplementation with a nutraceutical that provides vitamins, minerals, amino acids, and omega-3 for horses can increase sperm quality as well as the TAC of SP; however, this may be subject to the individual effect of each horse. Keywords: equine, nutraceutical, oxidative stress, sperm Journal: Czech Journal of Animal Science Pages: 450-461 Volume: 69 Issue: 11 Year: 2024 DOI: 10.17221/98/2024-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/98/2024-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202411-0003.txt Handle: RePEc:caa:jnlcjs:v:69:y:2024:i:11:id:98-2024-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Viktorija Nikonova Author-Workplace-Name: Latvia University of Life Sciences and Technologies, Jelgava, Latvia Author-Name: Daina Jonkus Author-Workplace-Name: Latvia University of Life Sciences and Technologies, Jelgava, Latvia Author-Name: Liga Paura Author-Workplace-Name: Latvia University of Life Sciences and Technologies, Jelgava, Latvia Title: Pedigree analysis of the Latvian Warmblood horse heavy type population Abstract: A breeding program has been developed for the Latvian warmblood heavy type horses in Latvia, with the aim to preserve this type, because the number of these horses is small and the status of the local endangered breed has been granted. The aim of the study was to analyse the genetic structure, inbreeding and effective population size of the Latvian warmblood heavy type (LSB) horse population. Horses that were living at the time of the study on January 1, 2023 were used for the study. A total of 374 genetic resources live horses were analysed. Population structure analysis, inbreeding and effective population size were investigated. Calculations were done by POPREP (v1.0) software. The effective population size (Ne) decreased, however, since 2018 the decline in the size of the effective population has not been so rapid. In 2000, the Ne was 316 animals, and in 2020, it was 101 animals. Over the years, changes in the average inbreeding level in the LSB population were observed from year to year. In 2000, inbreeding was 1.39%, and in 2020 it was 1.47% with the average inbreeding level of 1.12%. Over the last 20 years, large changes in Ne have been observed, thus, the loss of genetic diversity in the LSB breed should be controlled. Keywords: effective population, generation interval, inbreeding, horse breeding Journal: Czech Journal of Animal Science Pages: 462-469 Volume: 69 Issue: 11 Year: 2024 DOI: 10.17221/147/2024-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/147/2024-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202411-0004.txt Handle: RePEc:caa:jnlcjs:v:69:y:2024:i:11:id:147-2024-CJAS