Template-Type: ReDIF-Article 1.0 Author-Name: Roni Pazla Author-Workplace-Name: Department of Animal Nutrition, Faculty of Animal Science, Andalas University, Padang, Indonesia Author-Name: Antonius Antonius Author-Workplace-Name: Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong, Indonesia Author-Name: Zaitul Ikhlas Author-Workplace-Name: Department of Animal Nutrition, Faculty of Animal Science, Andalas University, Padang, Indonesia Author-Name: Anuraga Jayanegara Author-Workplace-Name: Department of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University, Bogor, Indonesia Author-Name: Laily Rinda Ardani Author-Workplace-Name: Department of Animal Nutrition, Faculty of Animal Science, Andalas University, Padang, Indonesia Author-Name: Maureen Chrisye Hadiatry Author-Workplace-Name: Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong, Indonesia Author-Name: Jumjunidang Jumjunidang Author-Workplace-Name: Research Center for Horticulture, National Research and Innovation Agency, Cibinong, Indonesia Author-Name: Tri Budiyanti Author-Workplace-Name: Research Center for Horticulture, National Research and Innovation Agency, Cibinong, Indonesia Author-Name: Firsoni Firsoni Author-Workplace-Name: Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong, Indonesia Author-Name: Ardinal Ardinal Author-Workplace-Name: Research Center for Agroindustry, National Research and Innovation Agency, Cibinong, Indonesia Author-Name: Kasma Iswari Author-Workplace-Name: Research Center for Agroindustry, National Research and Innovation Agency, Cibinong, Indonesia Author-Name: Dewi Febrina Author-Workplace-Name: Faculty of Agriculture and Animal Sciences, Sultan Syarif Kasim State Islamic University, Pekanbaru, Indonesia Author-Name: Eli Ratni Author-Workplace-Name: Department of Animal Production Technology, Faculty of Animal Science, Andalas University, Padang, Indonesia Title: The potential of tannin from Sonneratia alba fruit in defaunation of rumen protozoa and reducing methane gas production: An approach to increase livestock digestive efficiency Abstract: This research explores the addition of mangrove (Sonneratia alba) fruit to reduce the production of methane and the total population of protozoa. The dosage for adding mangrove fruit is 0% (without addition), 1.5%, 3%, and 4.5% in sugarcane tops-based feed. Results include ruminal product fermentation, gas and methane emissions, total protozoa, microbial protein production, microbial biomass, and nutrient digestibility. The research findings showed that an additional 1.5% to 4.5% dose can reduce methane gas emissions and the total number of protozoa. The total number of protozoa at 4.5% (T3) reached 9.89 × 104 cells/ml and methane gas was 56.1 ml/g DM (dry matter); 8.41 ml/g OM (organic matter). This effect is attributed to the tannin content in mangrove fruit, which exhibits antimicrobial properties. However, increasing doses also reduced nutrient digestibility. The findings suggest that incorporating 1.5-4.5% mangrove (Sonnetaria alba) fruit as a source of tannins causes a positive impact which reduces protozoa populations and methane production without changing the ruminal fermentation product. However, the addition of mangrove fruit in this study caused also reduced nutrient digestibility. Keywords: anti-nutrition, digestibility, greenhouse gas, ruminal fermentation, sugarcane tops Journal: Czech Journal of Animal Science Pages: 223-233 Volume: 70 Issue: 6 Year: 2025 DOI: 10.17221/130/2024-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/130/2024-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202506-0001.txt Handle: RePEc:caa:jnlcjs:v:70:y:2025:i:6:id:130-2024-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Khamanitjaree Saripan Author-Workplace-Name: Faculty of Science and Technology, Thepsatri Rajabhat University, Lopburi, Thailand Author-Name: Urailuck Pongket Author-Workplace-Name: Faculty of Agriculture and Technology, Rajamangala University of Technology Isan Surin Campus, Surin, Thailand Author-Name: Suriya Udduang Author-Workplace-Name: Faculty of Agriculture and Technology, Rajamangala University of Technology Isan Surin Campus, Surin, Thailand Author-Name: Supalug Kattakdad Author-Workplace-Name: Faculty of Agriculture and Technology, Rajamangala University of Technology Isan Surin Campus, Surin, Thailand Title: The role of spent coffee ground extracts as natural antioxidant supplements in the diet of Nile Tilapia (Oreochromis niloticus) Abstract: This study evaluated the potential of spent coffee grounds extract (CGE) as an antioxidant supplement in the diet of Nile tilapia (Oreochromis niloticus). Four experimental diets with varying CGE levels were formulated: 0% (CGE0, control), 2% (CGE2), 4% (CGE4), and 6% (CGE6). Nile tilapia (initial mean body weight = 38.65 ± 0.85 g) were cultured in fiberglass tanks at a stocking density of 20 fish/m2 under continuous aeration. The fish were fed three times daily at 5% of their total body weight. After an 8-week experimental period, the fish fed CGE-supplemented diets exhibited significantly higher growth rate compared to the control group, with CGE4 showing the most pronounced improvement in final body weight (P = 0.027) and weight gain (P = 0.050). However, no significant differences were observed in average daily growth (P = 0.054), specific growth rate (P = 0.256), survival rate (P = 0.487), and feed conversion ratio (P = 0.105) between the dietary treatments. Fish on the CGE6 diet exhibited the highest total plasma protein (P = 0.001) and immunoglobulin levels (P = 0.000). Moreover, CGE supplementation enhanced superoxide dismutase (P = 0.000) and glutathione peroxidase (P = 0.016) activities relative to the control group. The histopathological analysis showed significantly longer intestinal villi in fish fed CGE-supplemented diets, with the longest villi observed in the CGE6 group (P = 0.000). Fish fillets from the CGE6 group exhibited the highest springiness, while hardness was comparable between CGE6 and CGE4 but significantly higher than in CGE2 and CGE0. Additionally, CGE supplementation significantly influenced the colour expression, increasing lightness (L*) while decreasing redness (a*) and yellowness (b*) values. These findings indicate that 4% CGE supplementation is the most effective concentration, as it significantly promotes growth as evidenced by the highest weight gain, while also enhancing blood biochemical parameters, flesh quality, and antioxidative responses in Nile tilapia. Keywords: antioxidative activity, feed additive, fish feed, growth performance Journal: Czech Journal of Animal Science Pages: 242-252 Volume: 70 Issue: 6 Year: 2025 DOI: 10.17221/29/2025-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/29/2025-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202506-0002.txt Handle: RePEc:caa:jnlcjs:v:70:y:2025:i:6:id:29-2025-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Katalin Szendrő Author-Workplace-Name: Institute of Agricultural and Food Economics, Hungarian University of Agriculture and Life Sciences, Kaposvár, Hungary Title: Consumer perceptions of lab-grown cells: Awareness, barriers, and the power of information. A review Abstract: Lab-grown cells (also known as cell-based or cultured meat) are novel food innovations that face uncertain consumer acceptance. This review examines recent research (2020-2024) on consumer perceptions of lab-grown cells, focusing on three main areas: (1) public awareness and familiarity, (2) psychological and cultural aspects shaping perceptions, and (3) the impact of information on acceptance. Results show that the awareness of lab-grown cells varies widely across regions; in countries with high meat consumption, such as the USA and Australia, or where culinary delights are highly valued, such as France, respondents rated lab-grown cells lower than in those countries where meat consumption is now rising, for example, China or Mexico. The knowledge of lab-grown cells positively impacted attitude, while psychological barriers (e.g. food neophobia) and disgust emerged as key deterrents. The information provided to respondents significantly influenced their willingness to accept, buy, try, eat, and pay premium prices. Personal (mainly health-related) benefits significantly increased the acceptance of lab-grown cells, while societal benefits (e.g. benefits to the environment or animal welfare) had less prominent effects than expected. At the same time, information regarding production technology (high-tech, laboratory, artificial) resulted in lower ratings from respondents. The conclusion is that overlooking the positive attributes of meat and focusing on the unproven advantages of lab-grown cells may lead to misleading results. On the other hand, effective communication - especially messages highlighting personal benefits - can substantially improve the consumer openness. Keywords: cultured meat, meat, opinion, respondents, willingness Journal: Czech Journal of Animal Science Pages: 203-222 Volume: 70 Issue: 6 Year: 2025 DOI: 10.17221/31/2025-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/31/2025-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202506-0003.txt Handle: RePEc:caa:jnlcjs:v:70:y:2025:i:6:id:31-2025-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Gábor Mészáros Author-Workplace-Name: University of Natural Resources & Life Sciences Vienna, Vienna, Austria Author-Name: Birgit Fürst-Waltl Author-Workplace-Name: University of Natural Resources & Life Sciences Vienna, Vienna, Austria Author-Name: Johann Sölkner Author-Workplace-Name: University of Natural Resources & Life Sciences Vienna, Vienna, Austria Author-Name: Hana Vostrá-Vydrová Author-Workplace-Name: Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Jan Calta Author-Workplace-Name: Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Milena Đokić Author-Workplace-Name: Biotechnical Faculty, University of Montenegro, Podgorica, Montenegro Author-Name: Božidarka Marković Author-Workplace-Name: Biotechnical Faculty, University of Montenegro, Podgorica, Montenegro Author-Name: Dragan Stanojević Author-Workplace-Name: Faculty of Agriculture, University of Belgrade, Belegrade, Serbia Author-Name: Vladan Bogdanovic Author-Workplace-Name: Faculty of Agriculture, University of Belgrade, Belegrade, Serbia Author-Name: Nina Moravčíková Author-Workplace-Name: Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic Author-Name: Monika Chalupková Author-Workplace-Name: Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic Author-Name: Adrián Halvoník Author-Workplace-Name: Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic Author-Name: Radovan Kasarda Author-Workplace-Name: Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic Author-Name: Luboš Vostrý Author-Workplace-Name: Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Title: Genomic diversity characteristics of sheep populations in the Czech Republic, Slovakia, Serbia and Montenegro Abstract: Monitoring the genetic diversity in livestock is a critical component for sustainable management of small and endangered breeds. In this paper we follow up the two most often used metrics of genetic diversity, the average inbreeding coefficients (F) and the effective population (Ne) size in 16 sheep breeds from four countries. The analysis was based on single nucleotide polymorphism (SNP) markers, with about 35 to 49 thousand SNPs and 19 to 98 individuals after quality control. The runs of homozygosity (ROH) method with the cgaTOH software was used to estimate FROH, for three different time points in the past (3, 6 and 12 generations before present). Three methods were implemented to estimate Ne, using the NeEstimator v2, GONE and CurrentNe software for different time spans in the past. The average FROH ranged between 0.001 and 0.035 for 3 generations, 0.003 and 0.059 for 6 generations, 0.005 and 0.074 for 12 generations in the past. The wide range of inbreeding coefficients might be a consequence of different population sizes and breed management strategies in the respective countries. The results for Ne ranged from 7 to 352 for NeEstimator, 25 to 303 for GONE, and 15 to 542 for CurrentNe. Overall, the software showed comparable results for 10 out of 16 breeds, and significantly different results for at least one software for at least one of the methods for 6 out of 16 breeds. These differences show a degree of uncertainty in Ne estimations for certain breeds. Overall, we suggest the continued monitoring of sheep breeds, ideally by routine genotyping in all populations. This monitoring is especially important for small breeds with a greater decline in genetic diversity. Keywords: conservation status, genomic inbreeding, effective population size Journal: Czech Journal of Animal Science Pages: 234-241 Volume: 70 Issue: 6 Year: 2025 DOI: 10.17221/69/2025-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/69/2025-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202506-0004.txt Handle: RePEc:caa:jnlcjs:v:70:y:2025:i:6:id:69-2025-CJAS