Template-Type: ReDIF-Article 1.0 Author-Name: Metin Yazici Author-Workplace-Name: Department of Aquaculture, Faculty of Marine Sciences and Technology, Iskenderun Technical University, Hatay, Türkiye Title: Synergistic effects of dried Padina sp. and prebiotic on growth, histology, and growth-related gene expression in European seabass (Dicentrarchus labrax) Abstract: This study evaluated the effects of feeding European seabass (Dicentrarchus labrax) that developed from the fingerling to juvenile stage a diet supplemented with a blend of Padina sp. and GroBiotic®-A over a 12-week period. A total of 480 fish (initial weight: 2.08 ± 0.06 g) were randomly allocated to four groups, each comprising three replicates. The fish were fed to satiation with diets containing varying proportions of Padina sp. and GroBiotic®-A: 0% Padina sp. and 0% GroBiotic®-A (0P0G), 1% Padina sp. and 1% GroBiotic®-A (1P1G), 2% Padina sp. and 1% GroBiotic®-A (2P1G), and 4% Padina sp. and 1% GroBiotic®-A (4P1G). The 4P1G group demonstrated significant improvements in growth performance parameters, including final weight, weight gain, and specific growth rate (P < 0.05). However, no significant differences were found in other parameters including feed conversion ratio, visceral somatic index, and survival rate (P > 0.05). Histological analysis of liver and intestinal tissues showed no pathological alterations (P > 0.05); more likely, only adaptive and non-pathological morphological changes such as moderate lipid vacuolisation and preserved mucosal structure were noted. Furthermore, dietary supplementation of the Padina sp. and GroBiotic®-A blend significantly upregulated the expression of growth-related genes, specifically growth hormone and insulin-like growth factor 1 (P < 0.05). These results suggest that the combination of Padina sp. and GroBiotic®-A has a synergistic potential to enhance the growth performance without inducing digestive disturbances. Nevertheless, further studies are recommended to assess its effects on fish grown to market size before commercial application. Keywords: brown macroalgae, growth hormone, gut health, nutritional synergy Journal: Czech Journal of Animal Science Pages: 286-299 Volume: 70 Issue: 7 Year: 2025 DOI: 10.17221/194/2024-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/194/2024-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202507-0001.txt Handle: RePEc:caa:jnlcjs:v:70:y:2025:i:7:id:194-2024-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Klára Bartoňová Author-Workplace-Name: Department of Economics, Faculty of Economics and Management, Czech University of Life Sciences Prague, Prague - Suchdol, Czech Republic Author-Name: Jan Saro Author-Workplace-Name: Department of Systems Engineering, Faculty of Economics and Management, Czech University of Life Sciences Prague, Prague - Suchdol, Czech Republic Author-Name: Mansoor Maitah Author-Workplace-Name: Department of Economics, Faculty of Economics and Management, Czech University of Life Sciences Prague, Prague - Suchdol, Czech Republic Author-Name: Jaromír Ducháček Author-Workplace-Name: Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague - Suchdol, Czech Republic Title: Regional patterns and cluster analysis of agricultural methane emissions in the EU-27 countries Abstract: Agricultural methane emissions strongly contribute to global greenhouse gas production. Under these circumstances, meeting international climate goals, including the Global Methane Pledge or the European Green Deal, requires developing targeted mitigation strategies. However, research using advanced clustering techniques in a multilevel context remains scarce and mostly limited to CO2 emissions. This lack of time-series studies addressing regional variability hinders efforts to develop effective mitigation strategies. This study addresses three main research questions: (i) What are the main trends in agricultural methane emissions in the EU-27 countries from 2013 to 2022? (ii) How can the EU countries be classified based on agricultural methane emissions per capita? (iii) What is the impact of selected agricultural and economic indicators, including the number of live bovine animals and land use, on the clustering of methane emissions? Combining hierarchical and k-means clustering with trend analysis, this research integrates data from Eurostat and the World Bank, thereby classifying the EU-27 countries into four clusters based on their agricultural practices and methane emissions profiles. The results highlight distinct emission patterns across the EU-27 regions, with farming systems characterised by high stocking rates and intensive production generating the highest per capita emissions. By contrast, extensive systems with lower animal density exhibit reduced methane intensities. These findings underscore the need to devise effective, region-specific, data-driven policies and strategies for mitigating methane emissions. Keywords: emissions mitigation, global methane pledge, global warming, greenhouse gas, livestock, trend analysis Journal: Czech Journal of Animal Science Pages: 253-266 Volume: 70 Issue: 7 Year: 2025 DOI: 10.17221/26/2025-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/26/2025-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202507-0002.txt Handle: RePEc:caa:jnlcjs:v:70:y:2025:i:7:id:26-2025-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Maja Maurić Maljković Author-Workplace-Name: Department of Animal Breeding and Livestock Production, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: Tomislav Mašek Author-Workplace-Name: Department of Animal Nutrition and Dietetics, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: Marija Špehar Author-Workplace-Name: Croatian Agency for Agriculture and Food, Zagreb, Croatia Author-Name: Kristina Starčević Author-Workplace-Name: Department of Chemistry and Biochemistry, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Title: Effects of BRCA1 and TLR1 SNPs on milk production performance and somatic cell score in Holstein, Simmental and crossbred Holstein cattle Abstract: Mastitis is a complex, common and economically problematic issue in dairy cattle production. In this study, breast cancer 1 (BRCA1) and toll-like receptor 1 (TLR1) genes were taken as candidate genes for mastitis resistance. This study investigated whether BRCA1 and TLR1 genes were associated with milk production traits (daily milk yield, fat, and protein content) and somatic cell score (SCS). A total of 105 cows (25 Holstein, 48 Simmental, and 32 crossbred Holstein) were genotyped using the PCR-RFLP method. Cows with the BRCA1 c.46126G>T GG genotype had significantly lower SCS than the other genotypes. The TLR1 g.60438363C>T SNP influenced the protein content in all cows, with genotype CC having a higher content than TC. The same SNP in the Simmental breed showed that cows with the CC genotype had significantly higher SCS than the heterozygote. Cows with the GG genotype of TLR1 g.60437324A>G had significantly lower SCS and higher fat and protein content than the heterozygote. The results of this study indicate that BRCA1 c.46126G>T and TLR1 g.60437324A>G SNPs could be useful for improving mastitis resistance in dairy cattle through marker-assisted selection. Keywords: cow, mastitis, mastitis resistance, somatic cell count, udder health Journal: Czech Journal of Animal Science Pages: 267-275 Volume: 70 Issue: 7 Year: 2025 DOI: 10.17221/43/2025-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/43/2025-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202507-0003.txt Handle: RePEc:caa:jnlcjs:v:70:y:2025:i:7:id:43-2025-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Eva Straková Author-Workplace-Name: Department of Animal Husbandry, Animal Nutrition and Biochemistry, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: Lucie Všetičková Author-Workplace-Name: Department of Zoology, Fisheries, Hydrobiology and Apiculture, Faculty of AgriSciences, Mendel university in Brno, Brno, Czech Republic Author-Name: Pavel Suchý Author-Workplace-Name: Department of Animal Husbandry, Animal Nutrition and Biochemistry, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: Martin Kutlvašr Author-Workplace-Name: Department of Animal Husbandry, Animal Nutrition and Biochemistry, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Title: Effect of dehulled lupin seed meal in feed mixture on muscle fat quality of Cherry Valley ducks Abstract: The experiment was done on 180 (2 × 90) Cherry Valley ducks that were fattened until 42 days of age with parity of females and males in the group. The aim of the study was to examine the effect of substituting dehulled lupin seed meal, Zulika variety, for soybean meal and its impact on the fatty acid content of breast and thigh muscle fat. Feeding lupin diets resulted in a significant (P ≤ 0.05) increase in monounsaturated fatty acids (MUFA) in Cherry Valley ducks, as well as significant (P ≤ 0.05) increase in polyunsaturated fatty acids n-3 (PUFA n-3) in breast and thigh muscle fat. There was no effect of the tested diets on PUFA n-6 content in muscle fat. The results confirm that lupin meal in the diet enhances the dietary value of Cherry Valley duck muscle due to the higher proportion of unsaturated fatty acids, with respect to the use of duck meat for human consumption. Keywords: fattening, fatty acids, Lupinus albus, poultry Journal: Czech Journal of Animal Science Pages: 276-285 Volume: 70 Issue: 7 Year: 2025 DOI: 10.17221/76/2025-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/76/2025-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202507-0004.txt Handle: RePEc:caa:jnlcjs:v:70:y:2025:i:7:id:76-2025-CJAS