Template-Type: ReDIF-Article 1.0 Author-Name: Shang Gao Author-Workplace-Name: College of Agriculture, Yanbian University, Yanji, Jilin Province, P.R. China Author-Workplace-Name: Shanshi Farm, Beidahuang Group, Hailin, Heilongjiang Province, P.R. China Author-Name: Lina Hou Author-Workplace-Name: Yanbian Korean Autonomous Prefecture Animal Husbandry General Station, Yanji, Jilin Province, P.R. China Author-Name: Qingshan Gao Author-Workplace-Name: College of Agriculture, Yanbian University, Yanji, Jilin Province, P.R. China Author-Name: Haixing Liu Author-Workplace-Name: College of Agriculture, Yanbian University, Yanji, Jilin Province, P.R. China Title: Visualisation analysis of research frontiers, hotspots and trends in Fleckvieh breeding based on CiteSpace knowledge graph Abstract: This study investigates advancements and trends in Fleckvieh breeding research using the Web of Science Core Collection database to collate articles published since 1994. Through CiteSpace 6.4.R1, we conducted systematic analyses to visualise research trajectories, identify seminal domains, and delineate emerging patterns. Our methodology encompassed publication profiling, scientific collaboration network mapping, co-citation analysis, and keyword co-occurrence/clustering techniques. The findings reveal that the current research frontiers focus on: Genomic applications in genetic selection, Reproductive efficiency optimisation, Environmental adaptability mechanisms, Thermotolerance management strategies, Cross-disciplinary methodological integration. Germany, Czech Republic, Austria, and Switzerland emerged as leading contributors, with institutions including the Technical University of Munich and University of Natural Resources and Life Sciences, Vienna, demonstrating a particular scholarly influence. Knowledge mapping effectively delineated thematic concentrations and temporal evolution patterns, providing actionable insights for future research prioritisation. The field is progressing towards holistic, interdisciplinary frameworks underpinned by robust international consortia. This work introduces novel analytical perspectives to refine breeding protocols and enhance practical implementation. Keywords: bibliometric analysis in animal science, climate-resilient breeding, genomic selection application, heat stress mitigation, interdisciplinary breeding strategy, knowledge graph analysis Journal: Czech Journal of Animal Science Pages: 301-318 Volume: 70 Issue: 8 Year: 2025 DOI: 10.17221/32/2025-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/32/2025-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202508-0001.txt Handle: RePEc:caa:jnlcjs:v:70:y:2025:i:8:id:32-2025-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Zuzana Lacková Author-Workplace-Name: Department of Nutrition and Animal Husbandry, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovakia Author-Name: František Zigo Author-Workplace-Name: Department of Nutrition and Animal Husbandry, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovakia Author-Name: Zuzana Farkašová Author-Workplace-Name: Department of Nutrition and Animal Husbandry, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovakia Author-Name: Jakub Ješík Author-Workplace-Name: Clinic of Ruminants, University of Veterinary Medicine and Pharmacy in Košice, Košice, Košice, Slovakia Author-Name: Jana Záhumenská Author-Workplace-Name: Department of Hygiene, Technology and Health Food Safety, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovakia Author-Name: Silvia Ondrašovičová Author-Workplace-Name: Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovakia Author-Name: Andrej Récky Author-Workplace-Name: Department of Nutrition and Animal Husbandry, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovakia Author-Name: Henrieta Arpášová Author-Workplace-Name: Institute of Animal Husbandry, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic Title: Effect of dietary hop extracts and humic substances on the growth performance, carcass yield, blood biochemistry parameters, and meat quality of rabbits Abstract: This study aimed to evaluate the effects of hop extract in the form of xanthohumol (XA) and humic substances (HS) supplementation in the rabbit diet on growth performance, carcass traits, blood biochemical parameters, and the qualitative and sensory properties of rabbit meat. Experimental material consisted of 60 Big Light Silver × Chinchilla Giant crossbred rabbits, randomly assigned to three dietary groups. Experimental groups, XA and HS, were fed pellets enriched with 1.0% XA or 1.0% HS, respectively, for 63 days during the fattening period. Control group (C), consisting of rabbits that received a basal diet without supplements. In the XA group, the higher final live weight and carcass weight were recorded at the end of the fattening period, along with increased protein content and collagen-free muscle protein (BEFFE) in the leg and loin muscles, compared to the control group. In contrast, the HS-supplemented group exhibited the lower final live weight and carcass weight, along with reduced fat content in the leg and loin muscles, as reflected in lower blood cholesterol levels. Additionally, elevated blood serum calcium and phosphorus levels were observed in the HS group. Higher values of colourimetric parameters L* and h* (lightness and hue angle) were recorded in the leg meat of the XA group compared to the control group. Moreover, the results demonstrated that supplementation with 1.0% XA was more effective in delaying lipid oxidation in meat compared to the control group on the 7th day of refrigerated storage. In conclusion, the primary benefit of supplementing rabbit diets with 1% xanthohumol is improved growth performance, positively influenced by increased protein content, reduced fat content, and enhanced oxidative stability of the meat. Keywords: colour, muscle, organic matter, sensory, supplementation, weigh, xanthohumol Journal: Czech Journal of Animal Science Pages: 345-356 Volume: 70 Issue: 8 Year: 2025 DOI: 10.17221/82/2025-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/82/2025-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202508-0002.txt Handle: RePEc:caa:jnlcjs:v:70:y:2025:i:8:id:82-2025-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Weihua Chang Author-Workplace-Name: College of Animal Science, Xichang University, Xichang City, Sichuan, P.R. China Author-Workplace-Name: Key Laboratory of Animal Epidemic Disease Detection and Prevention in Panxi District of Sichuan, Xichang City, Sichuan, P.R. China Author-Name: Fengjiao Qiu Author-Workplace-Name: College of Animal Science, Xichang University, Xichang City, Sichuan, P.R. China Author-Name: Shanchuan Liu Author-Workplace-Name: College of Animal Science, Xichang University, Xichang City, Sichuan, P.R. China Author-Name: Guochao Ni Author-Workplace-Name: College of Animal Science, Xichang University, Xichang City, Sichuan, P.R. China Author-Name: Ying Zhao Author-Workplace-Name: College of Animal Science, Xichang University, Xichang City, Sichuan, P.R. China Author-Name: Yi Zhang Author-Workplace-Name: Key Laboratory of Local Characteristic Goat of Sichuan Province, Xichang City, Sichuan, P.R. China Author-Name: Guangwen Yan Author-Workplace-Name: College of Animal Science, Xichang University, Xichang City, Sichuan, P.R. China Author-Name: Juanhong Wang Author-Workplace-Name: College of Animal Science, Xichang University, Xichang City, Sichuan, P.R. China Title: Study on the expression patterns and biological functions of oar-miR-214_3p in the ovary and uterus of Liangshan Black Sheep Abstract: This study elucidates the expression dynamics and biological functions of oar-miR-214_3p in ovarian and uterine tissues of Liangshan Black Sheep across distinct stages of the oestrous cycle, providing novel insights into its regulatory mechanisms governing ovine reproductive physiology. The study employed RT-qPCR, northern blotting, FISH, dual-luciferase reporter assays, and ELISA to analyse the expression and biological functions of oar-miR-214_3p in the ovary and uterus of Liangshan Black Sheep across different stages of the oestrous cycle (proestrus, oestrus, metoestrus, and dioestrus phases). RT-qPCR and northern blotting revealed that oar-miR-214_3p is expressed in the ovarian and uterine tissues, with significant variations across different stages. Compared with the dioestrus and metoestrus phases, oar-miR-214_3p expression was significantly increased during the proestrus and oestrus phases (P < 0.05), with the highest levels observed during oestrus (P < 0.05). FISH analysis indicated that oar-miR-214_3p is primarily localised in the cytoplasm. We constructed wild-type and mutant vectors for mammalian target of rapamycin(mTOR) and Semaphorin 4D (Sema4D). After intervention for 48 h in granulosa cell cultures, RT-qPCR analysis of mTOR and Sema4D expression revealed that the mimic and inhibitor groups suppressed and promoted the expression of these target genes, respectively. The control and NC groups showed stable expression levels with no significant differences. Dual-luciferase reporter assay confirmed that mTOR and Sema4D are oar-miR-214_3p target genes. ELISA revealed that the mimic and inhibitor treatment groups promoted and suppressed oestrogen secretion, respectively. These findings confirm that oar-miR-214_3p, primarily localised in the cytoplasm, regulates ovarian follicle development, ovulation, oestrogen secretion, and the oestrous cycle via its target genes, mTOR and Sema4D. The study provides valuable insights into improving sheep reproductive performance through molecular breeding techniques. These findings have significant clinical and practical applications for enhancing reproductive efficiency. Keywords: cytoplasmic localisation, dual-luciferase assay, folliculogenesis, reproductive behaviour, steroidogenesis Journal: Czech Journal of Animal Science Pages: 335-344 Volume: 70 Issue: 8 Year: 2025 DOI: 10.17221/36/2025-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/36/2025-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202508-0003.txt Handle: RePEc:caa:jnlcjs:v:70:y:2025:i:8:id:36-2025-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Oto Hanuš Author-Workplace-Name: Dairy Research Institute Ltd., Prague, Czech Republic Author-Name: Hana Nejeschlebová Author-Workplace-Name: Dairy Research Institute Ltd., Prague, Czech Republic Author-Workplace-Name: Department of Food Biotechnologies and Agricultural Products' Quality, Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic Author-Name: Veronika Legarová Author-Workplace-Name: Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic Author-Name: Lucie Kejdová-Rysová Author-Workplace-Name: Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic Author-Name: Jan Říha Author-Name: Eva Samková Author-Workplace-Name: Department of Food Biotechnologies and Agricultural Products' Quality, Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic Author-Name: Gavin Thompson Author-Workplace-Name: Bentley Czech Ltd., Prague, Czech Republic Author-Name: Irena Němečková Author-Workplace-Name: Dairy Research Institute Ltd., Prague, Czech Republic Author-Name: Marcela Klimešová Author-Workplace-Name: Dairy Research Institute Ltd., Prague, Czech Republic Author-Name: Jaroslav Kopecký Author-Workplace-Name: Dairy Research Institute Ltd., Prague, Czech Republic Author-Name: Radoslava Jedelská Author-Workplace-Name: Dairy Research Institute Ltd., Prague, Czech Republic Title: The effect of artificial reduction of the somatic cell count, as a violation of authenticity, on milk quality indicators Abstract: The somatic cell count (SCC) in raw milk is an important indicator of health and hygienic quality. Artificial reduction of the SCC (ARSCC) in milk, for the apparent improvement of milk quality for commercial reasons, is an undesirable phenomenon and a violation of authenticity both in the factual sense and legislatively. Analytical methods need to be developed to identify ARSCC as well as to assess the effects that ARSCC may have on milk. The aim of the work was to quantify the effects of ARSCC on cow's milk as a food raw material. The results presented are some of the first on the given problem. Raw bulk tank cow milk was sampled in two experiments, each time for the whole year (2021-2022 and 2023-2024, n = 66 and n = 53, respectively) from herds of Czech Fleckvieh and Holstein cows, 1 : 1. ARSCC in experiment 1 (n = 66) slightly reduced the fat content, in experiment 2 (n = 53) it did not, otherwise the milk indicators with the exception of SCC were almost (1) and completely (2) identical. All samples were negative for the presence of inhibitory substance residues. ARSCC under the specified technological conditions reduced SCC (1) from 772 ± 906 103/ml to 376 ± 630 103/ml, by -51.3% (P < 0.001) and (2) from 592 ± 798 103/ml to 304 ± 468 103/ml, by -48.5% (P < 0.001). Under these circumstances, the milk fermentation ability improved (1) from 28.52 ± 4.72 °SH to 31.0 ± 4.65 °SH, by 8.66% (P < 0.001) and (2) from 32.51 ± 2.61 °SH to 33.80 ± 2.88 °SH, by 3.97% (P < 0.001). Curd firmness was better for SCC ≤ 400 103/ml compared to higher SCC by 5.26% (P < 0.001). Nevertheless, it is not possible, for hygienic and health reasons, to allow such milk for human consumption and it is necessary to find effective identification analytical methods for ARSCC. Keywords: centrifugation, milk composition, raw cow', s milk, subclinical mastitis, technological properties Journal: Czech Journal of Animal Science Pages: 319-334 Volume: 70 Issue: 8 Year: 2025 DOI: 10.17221/90/2025-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/90/2025-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202508-0004.txt Handle: RePEc:caa:jnlcjs:v:70:y:2025:i:8:id:90-2025-CJAS