Template-Type: ReDIF-Article 1.0 Author-Name: Yutong Liu Author-Workplace-Name: Agricultural College, Yanbian University, Yanji, P.R. China Author-Name: Jiaming Luan Author-Workplace-Name: Agricultural College, Yanbian University, Yanji, P.R. China Author-Name: Xin Feng Author-Workplace-Name: Agricultural College, Yanbian University, Yanji, P.R. China Author-Name: Jinying Zhou Author-Workplace-Name: Agricultural College, Yanbian University, Yanji, P.R. China Author-Name: Chunyin Geng Author-Workplace-Name: Agricultural College, Yanbian University, Yanji, P.R. China Author-Workplace-Name: Ministry of Education, Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Yanbian University, Yanji, P.R. China Title: Evaluation of the feeding value of microbially fermented cottonseed meal by in vitro method Abstract: In this study, different strains were used to ferment single/mixed cottonseed meal (cottonseed meal, corn flour, bran ratio of 7 : 2 : 1) substrates, and their feeding value was evaluated by an in vitro fermentation trial, to select suitable fermentation strains for cottonseed meal. For this purpose, two experiments (Exp.) were conducted. Exp. 1: Evaluation of the effect of fermentation of single cottonseed meal by different strains, which consisted of ten treatment groups: control (CON), Saccharomyces No. 1 (T1), Saccharomyces Fubon (T2), Saccharomyces Lallemand (T3), Lactobacillus (T4), Bacillus licheniformis (T5), Bacillus subtilis 10071 (T6), Bacillus subtilis 10089 (T7), Aspergillus niger (T8), and Monascus purpureus Went (T9). Exp. 2: Evaluation of the effect of fermentation of mixed cottonseed meal by different strains. The treatment groups were the same as in Exp. 1, numbered CON, M1, M2, M3, M4, M5, M6, M7, M8, and M9. The results showed that in both the single cottonseed meal group and the mixed cottonseed meal group free gossypol (FG) and methane (CH4) were significantly reduced and the gas production, total volatile fatty acids (TVFA), and in vitro digestibility of nutrients (P < 0.05) significantly increased, with Saccharomyces No. 1 showing the optimal effect. This study provides a theoretical basis for screening the suitable strains for fermenting cottonseed meal. Keywords: detoxification efficiency, free gossypol, in vitro gas production, microorganisms, rumen fermentation Journal: Czech Journal of Animal Science Pages: 208-219 Volume: 71 Issue: 5 Year: 2026 DOI: 10.17221/6/2025-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/6/2025-CJAS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202605-0001.txt Handle: RePEc:caa:jnlcjs:v:71:y:2026:i:5:id:6-2025-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Jan Saro Author-Workplace-Name: Department of Systems Engineering, Faculty of Economics and Management, Czech University of Life Sciences, Prague, Czech Republic Author-Name: Jaromír Ducháček Author-Workplace-Name: Department of Animal Husbandry, Faculty of Agrobiology, Food, and Natural Resources, Czech University of Life Sciences Prague, Czech Republic Author-Name: Luděk Stádník Author-Workplace-Name: Department of Animal Husbandry, Faculty of Agrobiology, Food, and Natural Resources, Czech University of Life Sciences Prague, Czech Republic Author-Name: Helena Brožová Author-Workplace-Name: Department of Systems Engineering, Faculty of Economics and Management, Czech University of Life Sciences, Prague, Czech Republic Title: Decision Support Systems in dairy cows farming: A 20-year scoping review of characteristics, applications, and future challenges Abstract: Decision Support Systems (DSS) streamline dairy farm management by addressing challenges in productivity, animal welfare, sustainability, and economics. Yet, their precise impact on dairy cattle farm operations remains unclear. This scoping review systematically analyses DSS applications in dairy farming using studies from Scopus and Web of Science published between 2005 and June 2025, following PRISMA-ScR guidelines. From 1 112 identified records, 84 studies were included, after deduplication and screening, and classified into four mutually exclusive primary categories, namely data-, model- and knowledge-driven and other specialised DSS. The findings revealed that DSS complexity increased over time, with model-driven systems dominating (40.5%), followed by data- (38.1%) and knowledge-driven (15.5%) DSS, while other specialised systems accounted for the remaining 6.0%. Temporal multi-label analysis also highlighted trends towards integrated methodologies, with 20 DSS combining data- and model-driven approaches. DSS are mainly applied in Animal Health and Welfare (48% model- and 32% data-driven) and in Farm Business and Management (54.5% model- and 22.7% data-driven). Consequently, the top data inputs are Animal Health & Performance (28.0%), Farm & Business (22.4%), and Environmental & Spatial Data (21.3%). The most commonly applied models are Mathematical/Deterministic (22.7%) and Simulation (13.6%) models, increasingly alongside ML techniques. Key challenges include data integration, real-farm validation, model interpretability, bias reduction, and practical usability. Bridging these gaps will enhance DSS effectiveness and strengthen their potential to optimise dairy farming. Keywords: animal health, dairy cows, decision support systems, farm management, machine learning, precision livestock farming, scoping review Journal: Czech Journal of Animal Science Pages: 191-207 Volume: 71 Issue: 5 Year: 2026 DOI: 10.17221/40/2026-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/40/2026-CJAS.html File-Format: text/html File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/40/2026-CJAS.pdf File-Format: Application/pdf X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202605-0002.txt Handle: RePEc:caa:jnlcjs:v:71:y:2026:i:5:id:40-2026-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Veronika Legarová Author-Workplace-Name: Department of Food Science, Czech University of Life Sciences Prague, Prague - Suchdol, Czech Republic Author-Name: Sona Formankova Herman Author-Workplace-Name: Department of Food Science, Czech University of Life Sciences Prague, Prague - Suchdol, Czech Republic Author-Name: Lucie Kejdova Rysova Author-Workplace-Name: Department of Food Science, Czech University of Life Sciences Prague, Prague - Suchdol, Czech Republic Author-Name: Zbynek Formanek Author-Workplace-Name: Department of Food Science, Czech University of Life Sciences Prague, Prague - Suchdol, Czech Republic Author-Name: Oto Hanus Author-Workplace-Name: Dairy Research Institute Ltd., Prague 6 - Vokovice, Czech Republic Author-Name: Katerina Bozikova Author-Workplace-Name: Department of Food Science, Czech University of Life Sciences Prague, Prague - Suchdol, Czech Republic Author-Name: Hana Nejeschlebova Author-Workplace-Name: Dairy Research Institute Ltd., Prague 6 - Vokovice, Czech Republic Author-Name: Jaromir Duchacek Author-Workplace-Name: Department of Food Science, Czech University of Life Sciences Prague, Prague - Suchdol, Czech Republic Author-Name: Matej Bozik Author-Workplace-Name: Department of Food Science, Czech University of Life Sciences Prague, Prague - Suchdol, Czech Republic Title: Detection of milk manipulation for the purpose of targeted reduction of somatic cell count using the MALDI-TOF MS method Abstract: This study examines methods for detecting illegal manipulation of raw cow's milk aimed at artificially reducing the somatic cell count (SCC) through centrifugation. This practice, motivated by economic gain, compromises the authenticity of the raw material and masks the true health status of the mammary gland. An experimental analysis of 68 samples demonstrated that centrifugation reduces somatic cell count by an average of 45.9 ± 11.6%. The MALDI-TOF MS method was used to identify changes in the peptide profile in the 500-4 000 Da range. Although no unique peaks specific to adulterated samples were detected, Pearson's correlation analysis revealed significant relationships between the intensity of specific peptides and SCC values. The strongest positive correlation was identified for peaks in the m/z 2 922 Da region (r = 0.69). Other relevant markers include fragments with m/z 1 768 Da (r = 0.51) and 901 Da (r = 0.49). The results confirm that monitoring quantitative changes in the intensity of specific peptides using mass spectrometry is a promising tool for identifying changes in the milk peptidome associated with variations in SCC, including those induced by technological manipulation. Keywords: dairy authenticity, MALDI-TOF MS, proteomic profiling, udder health indicators Journal: Czech Journal of Animal Science Pages: 227-235 Volume: 71 Issue: 5 Year: 2026 DOI: 10.17221/52/2026-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/52/2026-CJAS.html File-Format: text/html File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/52/2026-CJAS.pdf File-Format: Application/pdf X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202605-0003.txt Handle: RePEc:caa:jnlcjs:v:71:y:2026:i:5:id:52-2026-CJAS Template-Type: ReDIF-Article 1.0 Author-Name: Eva Straková Author-Workplace-Name: Department of Animal Breeding, Animal Nutrition and Biochemistry, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Author-Name: Natalie Klessy Author-Workplace-Name: Department of Animal Breeding, Animal Nutrition and Biochemistry, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic Title: Effect of cattle breed on muscle protein quality Abstract: The aim of the study was to compare the muscle protein quality in the m. longissimus, pars thoracis of bulls of the Aberdeen Angus (AA) and Czech Fleckvieh (CF) breeds reared under identical conditions within an extensive pasture-based system. The CF breed exhibited a higher crude protein content (887 ± 87.4 g/kg of dry matter) compared to AA (812 ± 59.3 g/kg of dry matter); P ≤ 0.05. A higher content of non-essential amino acids was recorded in CF (390 ± 37.9 g/kg of dry matter) compared to AA (364 ± 44.5 g/kg of dry matter); P ≤ 0.05, as well as higher contents of essential amino acids (CF 406 ± 38.9 g/kg of dry matter, AA 382 ± 41.0 g/kg of dry matter; P ≤ 0.05) and total analysed amino acids (CF 796 ± 74.8 g/kg of dry matter, AA 746 ± 82.7 g/kg of dry matter; P ≤ 0.05). Breed-related differences were observed in the content of most analysed amino acids (P ≤ 0.05), except for tyrosine and phenylalanine, for which no effect of breed was found (P > 0.05). Lysine showed the highest content among essential amino acids (AA 73.4 ± 8.23 g/kg of dry matter, CF 76.6 ± 8.87 g/kg of dry matter; P ≤ 0.05), while glutamic acid was dominant among non-essential amino acids (AA 109 ± 14.4 g/kg of dry matter, CF 119 ± 12.5 g/kg of dry matter; P ≤ 0.05). The ratio of essential to non-essential amino acids (EAA/NEAA) did not differ between breeds (AA 1.05 ± 0.08, CF 1.04 ± 0.05; P > 0.05). The results indicate that cattle breed affects the content of crude protein and the content of most analysed amino acids in the muscle tissue of AA and CF breeds; however, the EAA/NEAA ratio remains unaffected by breed. Keywords: beef, genotype, nutrients, nutritional quality, pasture Journal: Czech Journal of Animal Science Pages: 220-226 Volume: 71 Issue: 5 Year: 2026 DOI: 10.17221/55/2026-CJAS File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/55/2026-CJAS.html File-Format: text/html File-URL: http://cjas.agriculturejournals.cz/doi/10.17221/55/2026-CJAS.pdf File-Format: Application/pdf X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/cjs-202605-0004.txt Handle: RePEc:caa:jnlcjs:v:71:y:2026:i:5:id:55-2026-CJAS