Template-Type: ReDIF-Article 1.0 Author-Name: P. Havránek Author-Name: B. Navrátilová Author-Name: V. Chytilová Title: Use of rooted leaves for screening of Brassica germplasm response to clubroot (Plasmodiophora brassicae) and downy mildew (Hyaloperonospora parasitica) Abstract: Rooted leaves and cotyledons of various cruciferous crops were used for the screening of Brassica germplasm response to two obligatory pathogens: clubroot (Plasmodiophora brassicae) and downy mildew (Hyalope-ronospora parasitica). The development of roots was induced after 20-hour dipping of leaf pedicels in the mixture of growth regulators: indolyl-butyric acid (10 mg/l) and nicotinic acid (5 mg/l). The detached rooted leaves and cotyledons were maintained in 250ml plastic containers with perlite under fluorescent tubes in a growth chamber. With additional foliar fertilizing they remain vital for four months, producing clubroot galls on roots when dip-inoculated with Plasmodiophora spores and sporulating mycelia of downy mildew on leaves after drop inoculation with Hyaloperonospora parasitica. The possibilities of enhancing the sensitivity of this alternative assay in combination with immunochemical methods are discussed. Keywords: Brassica, clubroot, Plasmodiophora brassicae, downy mildew, Hyaloperonospora parasitica, rooted leaves Journal: Horticultural Science Pages: 1-5 Volume: 32 Issue: 1 Year: 2005 DOI: 10.17221/3758-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/3758-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200501-0001.txt Handle: RePEc:caa:jnlhor:v:32:y:2005:i:1:id:3758-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: J. Gubiš Author-Workplace-Name: Division of Applied Genetics and Breeding, Research Institute of Plant Production Piešťany, Piešťany, Slovak Republic Author-Name: Z. Lajchová Author-Workplace-Name: Division of Applied Genetics and Breeding, Research Institute of Plant Production Piešťany, Piešťany, Slovak Republic Author-Name: L. Klčová Author-Workplace-Name: Division of Applied Genetics and Breeding, Research Institute of Plant Production Piešťany, Piešťany, Slovak Republic Title: The effect of carbon source on plant regeneration in tomato Abstract: The effect of different tomato cultivars and different sugar types (sucrose, glucose and maltose) and concentrations (1.0, 2.0 and 3.0%) on shoot regeneration from aseptically grown hypocotyl and cotyledon explants were studied. Among sugar types, sucrose at a concentration of 3.0% induced the highest number of shoots from both types of explants. In hypocotyl explants, cv. Premium showed the best regeneration capacity (0.23 shoots per explant), and in cotyledon explants, cv. Hana produced the maximal number of shoots (0.43 or 0.37 for media with 2.0% or 3.0% sucrose, respectively). Keywords: in vitro culture, MS medium, sugars, organogenesis Journal: Horticultural Science Pages: 6-8 Volume: 32 Issue: 1 Year: 2005 DOI: 10.17221/3759-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/3759-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200501-0002.txt Handle: RePEc:caa:jnlhor:v:32:y:2005:i:1:id:3759-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: T. E. Sotiropoulos Author-Workplace-Name: N.AG.RE.F., Pomology Institute, Naoussa, Greece Author-Name: I. N. Therios Author-Workplace-Name: Department of Horticulture, Laboratory of Pomology, Aristotle University, Thessaloniki, Greece Author-Name: K. N. Dimassi Author-Workplace-Name: Department of Horticulture, Laboratory of Pomology, Aristotle University, Thessaloniki, Greece Author-Name: V. Tsirakoglou Author-Workplace-Name: Department of Horticulture, Laboratory of Pomology, Aristotle University, Thessaloniki, Greece Title: Effects of applications of a complex and N-Ca fertilizer on leaf and fruit nutrient concentrations and some fruit quality parameters in two apple cultivars Abstract: The aim of this research was to study the effects of application of 'hydrocomplex' (H) and 'Norway nitrate' (NN) fertilizers on leaf and fruit nutrient concentrations and some fruit quality parameters in the following apple (Malus domestica Borkh) cultivars (cvs.): Golden Delicious and Black Ben Davis. Applications of 50 kg NN, 75 kg NN, 50 kg NN plus 50 kg H and 75 kg NN plus 25 kg H per 0.1 hectare significantly increased leaf Ca concentration of the cv. Golden Delicious in comparison with the control in the July 15 sampling period. Boron concentration of leaves of the cv. Black Ben Davis increased steadily throughout the season. Applications of 75 kg H and 75 kg H plus 25 kg NN per 0.1 hectare significantly increased fruit B concentration of the cv. Black Ben Davis at harvest. The ratios N/Ca, K/Ca and K + Mg/Ca in fruit were calculated for all treatments. Applications of 25-75 kg NN, 25-75 kg H as well as the combination of the two fertilizers decreased the ratios N/Ca, K/Ca and K + Mg/Ca of fruits of the cvs. Golden Delicious and Black Ben Davis in comparison with the control at harvest. Applications of 50 kg (ΝΝ), 75 kg (Η), 25 kg (ΝΝ) plus 75 kg (H) and 75 kg (NN) plus 25 kg (H) per 0.1 hectare significantly increased soluble solids of fruits of the cv. Golden Delicious at harvest. Keywords: calcium, fruit firmness, Malus domestica, nutrient balance, soluble solids, storage quality Journal: Horticultural Science Pages: 9-16 Volume: 32 Issue: 1 Year: 2005 DOI: 10.17221/3760-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/3760-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200501-0003.txt Handle: RePEc:caa:jnlhor:v:32:y:2005:i:1:id:3760-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: K. Karlová Author-Workplace-Name: Faculty of Horticulture, Mendel University of Agriculture and Forestry, Brno, Lednice, Czech Republic Author-Name: K. Petříková Author-Workplace-Name: Faculty of Horticulture, Mendel University of Agriculture and Forestry, Brno, Lednice, Czech Republic Title: Variability of the content of active substances during Achillea collina Rchb. Alba ontogenesis Abstract: The fluctuation of essential oil, flavonoid and tannin content in Achillea collina Rchb. Alba (flos and herba millefolii) during ontogenesis was studied in this paper. Ten developmental stages of plants - from the beginning of flower differentiation to ripe seed stage - were tested for 3 years. The essential oil content was found to range between 0.4 and 1.6% of dry material and it was statistically significantly (one-factor analysis of variance; α = 0.05) influenced by developmental stage, harvested part of the plant and also by seasonal climatic conditions. The flavonoid content was influenced only by developmental stage and not by the type of yarrow drug or season. Its values ranged from 0.7 to 6.0% of dry material and it had a clear decreasing tendency during the plant ripening. Compared to flavonoids tannin production showed a reverse trend in yarrow. Their content was 0.3-1.0% and it increased during seed maturing. However differences between tannin contents during yarrow ontogenesis are not so dramatic and the influence of harvested part and season is also disputable. Comparison of the content of active substances in the drug from one- and six-years old yarrow plants did not indicate any differences due to age. Developmental stage V, beginning of flowering, can be recommended to producers as an optimum harvest stage because in this period the drug has the highest essential oil content in relation to plant mass yield and also to flavonoid and tannin contents. Keywords: Achillea collina, essential oils, flavonoids, tannins, variability, ontogenesis Journal: Horticultural Science Pages: 17-22 Volume: 32 Issue: 1 Year: 2005 DOI: 10.17221/3761-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/3761-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200501-0004.txt Handle: RePEc:caa:jnlhor:v:32:y:2005:i:1:id:3761-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: D. Kumar Author-Workplace-Name: Central Potato Research Institute Campus, Modipuram, India Author-Name: V. Paul Author-Workplace-Name: Indian Agricultural Research Institute, New Delhi, India Author-Name: R. Ezekiel Author-Workplace-Name: Central Potato Research Institute, Shimla, India Title: Chipping quality of potatoes stored in heaps and pits in subtropical plains of India Abstract: Two potato cultivars Kufri Bahar and Kufri Jyoti were stored in heaps and pits under ambient conditions of sub-tropical plains of northern India and their chipping quality was determined after 90 days of storage. During storage there was a significant reduction (33.84%) in the reducing sugar content of potatoes and improvement (31.63%) in chip colour. Sugar levels in stored potatoes were within the acceptable limit for processing and table purposes indicating that potatoes stored up to 90 days on the farm in heap and pits are suitable for these two purposes. Keywords: reducing sugars, chip colour, sucrose, dry matter content, potatoes and storage Journal: Horticultural Science Pages: 23-30 Volume: 32 Issue: 1 Year: 2005 DOI: 10.17221/3762-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/3762-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200501-0005.txt Handle: RePEc:caa:jnlhor:v:32:y:2005:i:1:id:3762-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: S. Kashyap Author-Workplace-Name: Centre for Rural Development and Technology, Indian Institute of Technology, Hauz Khas, New Delhi, India Author-Name: S. Sharma Author-Workplace-Name: Centre for Rural Development and Technology, Indian Institute of Technology, Hauz Khas, New Delhi, India Title: Role of bioinoculants and auxin in development of salt tolerant Mentha arvensis Abstract: Pot experiments were conducted for the development of salt tolerant Mentha arvensis (Japanese mint, family: Labiatae) saplings involving bioinoculants, namely Arbuscular Mycorrhizal (AM) fungi, Azotobacter and an auxin - Indole Acetic Acid (IAA). The IAA and sodium chloride (NaCl) concentrations were standardized prior to the experiments. The 10-ppm IAA and 0.08% NaCl (w/v) were found to be optimum in combination with AM fungi and Azotobacter to increase all the growth parameters and microbial count in the rhizosphere. For development of salt tolerant saplings, the optimal concentration of IAA, along with AM fungi and Azotobacter in different combinations, was applied in pots. The saplings were irrigated regularly with 0.08% NaCl water. Although plant growth, AM infection percentage, AM spores/100 g soil and Azotobacter cells/g soil were affected by NaCl watering, the inoculation of both bioinoculants significantly enhanced survival percentage of saplings from 10 to 40% under salt stress. Maximum survival (40%) of saplings was found with IAA (10 ppm) + AM fungi + Azotobacter treatment. Keywords: Mentha arvensis, in vivo, AM fungi, Azotobacter, IAA, NaCl stress Journal: Horticultural Science Pages: 31-41 Volume: 32 Issue: 1 Year: 2005 DOI: 10.17221/3763-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/3763-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200501-0006.txt Handle: RePEc:caa:jnlhor:v:32:y:2005:i:1:id:3763-HORTSCI