Template-Type: ReDIF-Article 1.0 Author-Name: G. Sujatha Author-Workplace-Name: Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India Author-Name: N. Jayabalan Author-Workplace-Name: Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India Author-Name: B. D. Ranjitha Kumari Author-Workplace-Name: Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India Title: Rapid in vitro micropropagation of Cicer arietinum L. Abstract: A rapid, simple and efficient protocol for in vitro multiple shoot induction and plantlet regeneration was achieved from three different explants of Cicer arietinum. The explants viz shoot tip, cotyledonary node and node were cultured on MS medium fortified with Benzyl Adenine (BA) (0.44-8.88μM) for multiple shoot induction. Multiple shoots proliferation was best observed at 4.44μM BA from all the three explants within two weeks of culture. Of the three different explants tested, cotyledonary nodes produced the maximum number of shoots. Shoot number per explant ranged between 7 and 15. Individual shoots were aseptically excised and subcultured in the same media for shoot elongation. The elongated shoots were transferred to Indole Butyric Acid (IBA) (2.46-12.30μM) for root induction. Rooting was observed within two weeks of culture. Rooted plantlets were successfully hardened under culture conditions and subsequently established in the field conditions. The recorded survival rate of the plants was 76.3%. Plants looked healthy with no visually detectable phenotypic variations. Keywords: shoot tip, cotyledonary node, node, multiple shoots, rooting, hardening Journal: Horticultural Science Pages: 1-5 Volume: 34 Issue: 1 Year: 2007 DOI: 10.17221/1842-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/1842-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200701-0001.txt Handle: RePEc:caa:jnlhor:v:34:y:2007:i:1:id:1842-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: M. Koudela Author-Workplace-Name: Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: K. Petříková Author-Workplace-Name: Faculty of Horticulture, Mendel University of Agriculture and Forestry Brno, Lednice, Czech Republic Title: Nutritional composition and yield of endive cultivars - Cichorium endivia L. Abstract: Two year trials at the Horticultural Faculty in Lednice (part of the Mendel University of Agriculture and Forestry Brno) evaluated six cultivars of endive (Cichorium endivia L.): 3 cultivars of C. endivia L. var. crispum (Markant, Midori, Protos), and 3 cultivars of C. endivia L. var. latifolium (Malan, Maral, Nuance). At harvest time, the nutritional composition was evaluated: levels of vitamin C, minerals (K, Na, Ca, Mg), chlorophyll, dietary fibre, dry matter and nitrates. In addition, a single plant weight and yield were measured. It was observed that the cultivars of C. endivia var. crispum contained significantly higher quantities of dietary fibre and dry matter than those of C. endivia L. var. latifolium. Nitrate levels were significantly higher in the leaves of C. endivia L. var. latifolium cultivars. Except for vitamin C and dietary fibre, it was observed that all the investigated parameters varied significantly from one year to the next. Out of the six cultivars of endive, the Maral cultivar showed the highest average yield per 100 m2 (662 kg). Keywords: endive (Cichorium endivia L.), nutritional content, yield, weight Journal: Horticultural Science Pages: 6-10 Volume: 34 Issue: 1 Year: 2007 DOI: 10.17221/1848-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/1848-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200701-0002.txt Handle: RePEc:caa:jnlhor:v:34:y:2007:i:1:id:1848-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: T. Pokorná-Juríková Author-Workplace-Name: Department of Elementary Didactics, Faculty of Central European Studies, Constantine the Philosopher University Nitra, Nitra, Slovak Republic Author-Name: J. Matuškovič Author-Workplace-Name: Department of Fruit Production, Viticulture, and Enology, Slovak University of Agriculture, Nitra, Slovak Republic Title: The study of irrigation influence on nutritional value of Lonicera kamtschatica - cultivar Gerda 25 and Lonicera edulis berries under the Nitra conditions during 2001-2003 Abstract: Two species of edible honeysuckle were studied in the trial established in the autumn 1994. The spacing used was 2.0 × 1.5 m and the experiment included irrigated as well as non- irrigated variants. The aim of this study was to provide a summary of nutritional values of Lonicera kamtschatica and Lonicera edulis berries under the Nitra conditions during the period 2001-2003. The berries were examined for the content of dry mater, sugar, organic acids, ascorbic acid, mineral substances - K, Ca, P, Mg, Na and anthocyanins from pomace and juice. The results of our experiments refer to the exceptionally high content of ascorbic acid ranging from 28.56 up to 86.96 mg/100 g, potassium 10,175 to 14,764 mg/kg and anthocyanins from pomace 6.245-17.36 g/kg. The values of ascorbic acid varied and they were statistically significantly depending on the year; similarly, only year can be taken into account as a statistically significant factor for forming content of sugar, magnesium and sodium. The content of dry mater, organic acids, potassium, calcium and anthocyanins in juice was influenced not only by year but also by species. The interaction species - year as a source of statistically significant differences is evident in the case of phosphorus and organic acids. A significant influence of irrigation on all evaluated nutrients was not confirmed. Keywords: nutritional value of fruits, Lonicera kamtschatica (Sevast.) Pojark., Lonicera edulis Turcz. ex Freon Journal: Horticultural Science Pages: 11-16 Volume: 34 Issue: 1 Year: 2007 DOI: 10.17221/1841-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/1841-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200701-0003.txt Handle: RePEc:caa:jnlhor:v:34:y:2007:i:1:id:1841-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: K. Dušek Author-Workplace-Name: Department of Vegetables and Special Crops Olomouc, Crop Research Institute, Czech Republic Author-Name: B. Galambosi Author-Workplace-Name: Agrifood Research Finland, Ecological Production, Mikkeli, Finland Author-Name: E. B. Hethelyi Author-Workplace-Name: Department of Medicinal and Aromatical Plants, CORVINUS University, Budapest, Hungary Author-Name: K. Korany Author-Workplace-Name: Department of Food Chemistry and Nutrition, CORVINUS University, Budapest, Hungary Author-Name: K. Karlová Author-Workplace-Name: Department of Vegetables and Special Crops Olomouc, Crop Research Institute, Czech Republic Title: Morphological and chemical variations of sweet flag (Acorus calamus L.) in the Czech and Finnish gene bank collection Abstract: The aim of this study was to analyze both the morphological and chemical characteristics of Acorus calamus plants collected from the natural sites in the Czech Republic and to compare them to the sweet flags growing in Finland, Slovenia and Canada, which are concentrated in the Gene Bank collections in Finland. The sweet flags of Finnish, Slovenian and Czech origin were found to be phenotypically and chemically very similar. They all represent the European triploid type of Acorus calamus var. calamus. The average essential oil content was established at the value of 1.50% of dry material in Finland and 1.91% in the Czech Republic. This amount is not sufficient according to the Czech norm for Radix calami quality (minimal amount is 2%) but the content of carcinogen β-asarone, which was established at 9-21% in the essential oil in all the analyzed sweet flags, did not exceed the maximal recommended value 0.5% of dry drug mass. Keywords: Acorus calamus, variability, essential oil, β -asarone Journal: Horticultural Science Pages: 17-25 Volume: 34 Issue: 1 Year: 2007 DOI: 10.17221/1847-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/1847-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200701-0004.txt Handle: RePEc:caa:jnlhor:v:34:y:2007:i:1:id:1847-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: J. Goliáš Author-Workplace-Name: Horticultural Faculty, Mendel University of Agriculture and Forestry Brno, Lednice, Czech Republic Author-Name: A. Němcová Author-Workplace-Name: Horticultural Faculty, Mendel University of Agriculture and Forestry Brno, Lednice, Czech Republic Author-Name: A. Čaněk Author-Workplace-Name: Horticultural Faculty, Mendel University of Agriculture and Forestry Brno, Lednice, Czech Republic Author-Name: D. Kolenčíková Author-Workplace-Name: Horticultural Faculty, Mendel University of Agriculture and Forestry Brno, Lednice, Czech Republic Title: Storage of sweet cherries in low oxygen and high carbon dioxide atmospheres Abstract: Acetaldehyde and ethanol are already present in detectable levels at the time of harvest, under aerobic conditions and in healthy, undamaged fruit. Both metabolites can be detected, at different concentrations, in all cultivars. Several hours after harvest, the levels of acetaldehyde in cultivars Summit, Techlovan and Kordia were 6.41, 9.78 and 22.00 mg/l, respectively. Both ethanol and acetaldehyde accumulate to significant levels in anaerobically stored cherries, particularly in atmospheres with high levels of CO2. The highest levels of ethanol observed, after 31 days of exposure to anaerobic conditions, were in the cultivars Techlovan (1,159 mg/l) and Summit (1,168 mg/l); both concentrations are are broadly similar. The metabolites decreased after a return to aerobic conditions, but remained higher than the levels first observed. Sweet cherries stored in anaerobic conditions are also sensitive to the development of off-flavours in the first 24 hours after opening the storage box. The very slow ripening of the fruit under anaerobic conditions was satisfactorily quantified by measuring the higher degree of fruit firmness, when the usual, visual attributes of ripeness were almost undetectable. Stems also remained green, in contrast to the usual browning that occurs under normal atmospheres. Discrimination analysis of various parameters observed gave a good resolution of different cultivars. Keywords: ethanol, acetaldehyde, firmness, sweet cherry, off-flavour Journal: Horticultural Science Pages: 26-34 Volume: 34 Issue: 1 Year: 2007 DOI: 10.17221/1843-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/1843-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200701-0005.txt Handle: RePEc:caa:jnlhor:v:34:y:2007:i:1:id:1843-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: F. Šrámek Author-Workplace-Name: Silva Tarouca Research Institute for Landscape and Ornamental Gardening, Průhonice, Czech Republic Author-Name: M. Dubský Author-Workplace-Name: Silva Tarouca Research Institute for Landscape and Ornamental Gardening, Průhonice, Czech Republic Title: Effect of slow release fertilizers on container-grown woody plants Abstract: Slow-Release Fertilizers (SRF) Silvamix Forte and Silvagen were tested in two-year experiments with container-grown woody plants (Pyracantha coccinea, Thuja occidentalis). Several fertilizing systems were compared: preplant application of SRF into substrate as the sole nutrient source for a two-year period, preplant application of SRF and soluble fertilizer (PG Mix), and preplant application of SRF and soluble fertilizer together with additional fertilizing by solution of nitrogen fertilizer during both growing periods. A system with controlled-release fertilizer (CRF) Osmocote 5-6 was chosen as a control variant; it was incorporated into substrate before planting in the first year and top-dressed in the second year. CRF Plantacote 6M (mixed into substrate before planting and top-dressed in the second year) and Osmocote 16-18 applied only before planting were tested, too. The experiments showed that SRF Silvamix Forte and Silvagen give results comparable with CFR provided that they were incorporated together with soluble ferti-lizer dose and plants were fertilized by solution of nitrogen fertilizer during both growing periods. Keywords: slow release fertilizers, controlled-release fertilizer, woody plants, growth response Journal: Horticultural Science Pages: 35-41 Volume: 34 Issue: 1 Year: 2007 DOI: 10.17221/1844-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/1844-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200701-0006.txt Handle: RePEc:caa:jnlhor:v:34:y:2007:i:1:id:1844-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: J. Mareček Author-Workplace-Name: Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Title: Folk landscape architecture as a significant value of Czech landscape Abstract: In the past the image of Czech countryside was created by agricultural and social activities of the rural population in a significant manner. These activities related to natural elements and to the creation of landscape in a wider sense can be described as folk landscape architecture. Its object is mainly the spatial arrangement and assortment composition of vegetation and its functionality in villages and in their landscape environment. This study defines these activities as time limited regional (local) customary practices of agricultural and cultural and social character, reflected especially in the spatial arrangement and assortment composition of vegetation elements. Vegetation and other natural elements are evaluated as functional singularities and as functional systems in relation to particular structures, type of village pattern and state of the surrounding landscape. Besides the methodical categorisation of evaluated objects principles for their use in different forms of land-use planning are defined. A significant result of this study is the definition of landscape architecture as a phenomenon of the rural population lifestyle in which not only the past but also the future of rural landscape is reflected. Keywords: landscape, landscape character, village, landscape architecture, permanent vegetation, tree species, customary praktice Journal: Horticultural Science Pages: 42-46 Volume: 34 Issue: 1 Year: 2007 DOI: 10.17221/1846-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/1846-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200701-0007.txt Handle: RePEc:caa:jnlhor:v:34:y:2007:i:1:id:1846-HORTSCI