Template-Type: ReDIF-Article 1.0
Author-Name: M. Koudela
Author-Workplace-Name: Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
Author-Name: K. Petříková
Author-Workplace-Name: Faculty of Horticulture, Mendel University of Agriculture and Forestry in Brno, Lednice, Czech Republic
Title: Nutritional compositions and yield of sweet fennel cultivars - Foeniculum vulgare Mill. ssp. vulgare var.azoricum (Mill.) Thell
Abstract: In two-year trials taking place at the Faculty of Horticulture in Lednice (Mendel University of Agriculture and Forestry in Brno), three cultivars of sweet fennel (Foeniculum vulgare Mill. ssp.vulgare var. azoricum [Mill.] Thell) - Precoce di Bologna, Rudy F1 and Zefa Fino have been evaluated. The trials were conducted in two terms in each of the trial years (summer and autumn term). In the harvest time, nutritional value was evaluated: content of vitamin C, minerals (K, Na, Ca, Mg), dietary fibre, dry matter and nitrates. Besides, the weights of pseudobulbs and its yield have been evaluated. The content of the monitored substances ranged in the following intervals: vitamin C 87-347 mg/kg, K 4,241-5,851 mg/kg, Na 77-512 mg/kg, Ca 56-363 mg/kg, Mg 82-389 mg/kg, dietary fibre 5.75-7.59 g/kg, dry matter 61-75.8 g/kg, nitrates 650 to 3,767 mg/kg, weight of pseudobulb 199-383 g. A significant influence of cultivar on K content has been observed; furthermore, growing term markedly influenced the content of vitamin C, K, Na, Ca, Mg, dietary fibre, nitrates and on weight of pseudobulb. A significant influence of year on the content of vitamin C, Ca, Mg, dietary fibre, dry matter, nitrates and weight of pseudobulb has been recorded as well. From the three tested cultivars, Zefa Fino showed significantly higher average yield (331 kg/100 m2) in summer terms and Rudy F1 (267 kg/100 m2) in autumn terms.
Keywords: sweet fennel (Foeniculum vulgare Mill. ssp.vulgare var.azoricum [Mill.] Thell), Precoce di Bologna, Rudy F1, Zefa Fino, vitamin C (ascorbic acid), minerals (K, Na, Ca, Mg), dietary fibre, dry matter, nitrates, weight of pseudobulb
Journal: Horticultural Science
Pages: 1-6
Volume: 35
Issue: 1
Year: 2008
DOI: 10.17221/644-HORTSCI
File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/644-HORTSCI.html
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Template-Type: ReDIF-Article 1.0
Author-Name: T. E. Sotiropoulos
Author-Workplace-Name: N.AG.RE.F., Pomology Institute, Naoussa, Greece
Title: Performance of the apple (Malus domestica Borkh) cultivar Imperial Double Red Delicious grafted on five rootstocks
Abstract: The performance of the apple (Malus domestica Borkh) cultivar Imperial Double Red Delicious (Imperial D.R.D.) grafted on the following 5 rootstocks: (I) seedling, (ii) M 7, (ii) MM 104, (ivv) MM 109 was investigated. The highest yield per tree was recorded for trees on seedling rootstock, while the lowest for the cultivar grafted on M 7. Production efficiency of Imperial D.R.D. was higher when grafted on MM 106 and M 7, intermediate on seedling and MM 104, and the lowest on MM 109. The highest leaf photosynthetic rate (Pn) was measured for scions grafted on seedling although it was not significantly different from MM 109. Nitrogen concentrations of leaves of the cv. Imperial D.R.D. grafted on M 7 and MM 106 were significantly lower in comparison to the seedling variant. Flesh firmness at harvest, pH of juice at harvest, soluble solids content and titratable acidity at harvest and after four months of storage were not significantly different among the rootstocks.
Keywords: apple, fruit quality, mineral uptake, photosynthetic rate, production efficiency, rootstocks
Journal: Horticultural Science
Pages: 7-11
Volume: 35
Issue: 1
Year: 2008
DOI: 10.17221/645-HORTSCI
File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/645-HORTSCI.html
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Template-Type: ReDIF-Article 1.0
Author-Name: Dj. V. Ružić
Author-Workplace-Name: Fruit Research Institute, Čačak, Republic of Serbia
Author-Name: T. I. Vujović
Author-Workplace-Name: Fruit Research Institute, Čačak, Republic of Serbia
Title: The effects of cytokinin types and their concentration on in vitro multiplication of sweet cherry cv. Lapins (Prunus avium L.)
Abstract: Determination of the most optimal types and concentrations of plant growth regulators as medium constituents is one of the most important aspects of successful micropropagation, among other in vitro factors. With the aim of optimization of in vitro multiplication of sweet cherry cv. Lapins the effect of following cytokinins has been studied: benzyladenine (BA), isopentenyl adenine (2iP), kinetin (KIN) and thidiazuron (TDZ) at concentrations of 1, 2, 5, 10 and 15μM, combined with auxine, indole-3-butyric acid (IBA) at concentrations of 0, 0.5, 2.5 and 5μM. MURASHIGE and SKOOG (1962) was the basic medium used in all the combinations. The following multiplication parameters were monitored: multiplication index, length of axial and lateral shoots. Fresh and dry shoot weight (callus, stem and leaves - S + L) were determined. Some specific issues, such as colour, leaf and callus size, leaf roll, incidence of chlorosis or necrosis along with occurrence of rhizogenesis, i.e. roots unusual for this phase of micropropagation, were also monitored. The highest multiplication index as well as length of axial and lateral shoots was obtained on media with BA. Very poor multiplication, with large sized shoots and big leaves, was achieved on media with 2iP, TDZ and KIN, whereas in many combinations with 2iP, and particularly in those with KIN, rhizogenesis was induced. Obtained results suggest that the choice of cytokinins for the phase of multiplication of sweet cherry is limited to BA. For more rapid micropropagation, through joining rooting and multiplication phases, KIN and 2iP may be applied. The latter two may be also used to obtain sturdy shoots (elongation phase, prior to rooting).
Keywords: cytokinins, Prunus avium L., in vitro multiplication, root induction
Journal: Horticultural Science
Pages: 12-21
Volume: 35
Issue: 1
Year: 2008
DOI: 10.17221/646-HORTSCI
File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/646-HORTSCI.html
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Template-Type: ReDIF-Article 1.0
Author-Name: G. R. Rout
Author-Workplace-Name: Plant Biotechnology Division, Regional Plant Resource Centre, Orissa, India
Author-Name: S. K. Senapati
Author-Workplace-Name: Plant Biotechnology Division, Regional Plant Resource Centre, Orissa, India
Author-Name: S. Aparajeta
Author-Workplace-Name: Plant Biotechnology Division, Regional Plant Resource Centre, Orissa, India
Title: Micropropagation of Acacia chundra (Roxb.) DC.
Abstract: An in vitro propagation of an economic leguminous tree, Acacia chundra, has been standardized. Induction of bud sprout was obtained from shoot tip and nodal explants derived from in vitro grown plants of A. chundra on the Murashige and Skoog (MS) basal medium supplemented with 6-benzylaminopurine (BA) (1.0 mg/l) and 20 mg/l adenine sulfate (Ads). The rate of multiplication was obtained on MS medium supplemented with 1.5 mg/l BA, 0.01 to 0.05 mg/l (indole-3-acetic acid) IAA and 50 mg/l Ads. The multiplication rate varied from 3 to 6 shoots depending on the growth regulators used. Excised shoots were rooted on half-strength MS basal salts supplemented with 0.25 mg/l indole-3-butyric acid (IBA) or IAA and 20 g/l (w/v) sucrose after 10 to 12 days of culture. The micropropagated plantlets have been acclimatized and successfully transferred to soil.
Keywords: growth regulators, in vitro, legume tree, micropropagation
Journal: Horticultural Science
Pages: 22-26
Volume: 35
Issue: 1
Year: 2008
DOI: 10.17221/648-HORTSCI
File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/648-HORTSCI.html
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Template-Type: ReDIF-Article 1.0
Author-Name: S. K. Senapati
Author-Workplace-Name: Plant Biotechnology Division, Regional Plant Resource Centre, Orissa, India
Author-Name: G. R. Rout
Author-Workplace-Name: Plant Biotechnology Division, Regional Plant Resource Centre, Orissa, India
Title: Study of culture conditions for improved micropropagation of hybrid rose
Abstract: An efficient protocol was developed for micropropagation of hybrid roses by manipulating growth regulators, photoperiods, gelling agent and subculture period. Multiple shoots were achieved from nodal explants of Rosa hybrida cvs. Cri Cri, Pariser Charme and First Red on the Murashige and Skoog (MS) medium supplemented with 1.5-2.0 mg/l BA (6- benzylaminopurine), 50 mg/l Ads (adenine sulfate) with 3% (w/v) sucrose. Inclusion of indole-3-acetic acid (IAA; 0.1-0.25 mg/l) into the cytokinin-rich medium promoted high frequency of shoot multiplication. The induction of multiple shoots was also affected by photoperiod and subculture period. Higher multiplication was achieved under 16 h photoperiod in all tested cultivars. The rate of multiplication was low when photoperiod both increased or decreased. The frequency of shoot multiplication was best up to the 6th to 7th subculture and thereafter it declined. Rooting was readily achieved upon transferring the microshoots onto half-strength MS medium supplemented with 0.25 mg/l IBA (indole-3-butyric acid) and 2% (w/v) sucrose. The percentage of rooting was less on MS medium containing NAA (1-naphthalene acetic acid) or IAA as compared with IBA. More than 60% of rooted plantlets were established in the greenhouse. The in vitro raised plantlets were grown normally and flowered within one month after their transfer to open field.
Keywords: growth regulators, in vitro, rose, shoot multiplication
Journal: Horticultural Science
Pages: 27-34
Volume: 35
Issue: 1
Year: 2008
DOI: 10.17221/650-HORTSCI
File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/650-HORTSCI.html
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Template-Type: ReDIF-Article 1.0
Author-Name: J. K. Kanwar
Author-Workplace-Name: Department of Biotechnology, University of Horticulture & Forestry, Solan, India
Author-Name: S. Kumar
Author-Workplace-Name: Department of Biotechnology, University of Horticulture & Forestry, Solan, India
Title: In vitro propagation of Gerbera: A Review
Abstract: Gerbera has gained popularity in the past few years in many countries of the world and it is in great demand in the floral industry as cut flower as well as potted plant due to its beauty, colour, long vase life, and ability to rehydrate after long transportation. The most commercial cultivars are propagated through vegetative means by multiplication through divisions of clumps; however, the multiplication by this method is too slow to be commercially viable. To commercialize this crop and to meet the growing demand for planting material, tissue and organ culture techniques are being used as alternative methods for propagation in many countries. Most of the work has been carried on plant regeneration by adventitious organogenesis from capitulum, shoot tip, leaf, petiole and other parts of the plant. Attention should be paid to improve the technology to achieve 100% success in all species/cultivars to meet growing demands of the growers globally. From the literature, it is evident that gerberas are highly amenable to in vitro studies, as various explants were found to favourably respond to different culture media with different types and concentrations of growth regulators.
Keywords: micropropagation, explants, Gerbera, growth regulators
Journal: Horticultural Science
Pages: 35-44
Volume: 35
Issue: 1
Year: 2008
DOI: 10.17221/651-HORTSCI
File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/651-HORTSCI.html
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