Template-Type: ReDIF-Article 1.0 Author-Name: J. Sedlák Author-Workplace-Name: Research and Breeding Institute of Pomology Holovousy Ltd., Holovousy, Czech Republic Author-Name: F. Paprštein Author-Workplace-Name: Research and Breeding Institute of Pomology Holovousy Ltd., Holovousy, Czech Republic Title: In vitro shoot proliferation of sweet cherry cultivars Karešova and Rivan Abstract: The objective of this study was to investigate the possibility of optimizing routine tissue culture methods to proliferate two sweet cherry cultivars Karešova and Rivan. Shoot tips of two genotypes were successfully established in vitro. Six proliferation MS media containing 1, 2 and 4 mg/l BAP (6-benzylaminopurine), 0.5 and 1 mg/l TDZ (thidiazuron) or 10 mg/l 2iP (6-(γ, γ-dimethylallylamino)purine) were tested. The highest proliferation rate (3.0 ± 0.1) was obtained for Rivan on MS medium containing 2 mg/l BAP. In the case of cultivar Karešova, any of the cytokinins tested did not promote satisfactory proliferation. The highest proliferation rate (1.6) achieved on MS medium with 2 mg/l 2iP is not sufficient for a larger scale in vitro shoot production. It was proved that different genotypes of sweet cherry do not respond in the same way during proliferation in vitro. Future research and testing of other media and plant growth regulators will be carried out. Keywords: Prunus avium micropropagation, culture initiation, multiplication, cytokinins Journal: Horticultural Science Pages: 95-98 Volume: 35 Issue: 3 Year: 2008 DOI: 10.17221/6/2008-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/6/2008-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200803-0001.txt Handle: RePEc:caa:jnlhor:v:35:y:2008:i:3:id:6-2008-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: M. Koudela Author-Workplace-Name: Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: K. Petříková Author-Workplace-Name: Mendel University of Agriculture and Forestry in Brno, Brno, Czech Republic Title: Nutrients content and yield in selected cultivars of leaf lettuce (Lactuca sativa L. var. crispa) Abstract: Five cultivars of leaf lettuce (Lactuca sativa L. var. crispa) - Bergamo, Dubáček, Frisby, Lollo Rossa and Redin - were evaluated in two-year experiments carried out at the Faculty of Horticulture in Lednice (Mendel University of Agriculture and Forestry in Brno). Experiments were conducted in two trial years, 1998 and 1999; the lettuce was cultivated in three seasons: spring, summer and autumn. After the harvest, contents of following nutrients were evaluated: vitamin C, minerals (K, Na, Ca, Mg), fibre, dry matter and nitrates. The weight of leaf rosette was also recorded. The contents of selected substances and weights of leaf rosette were ranged as follows: vitamin C (65 to 302 mg/kg), potassium (2,394 to 6,477 mg/kg), sodium (39 to 223 mg/kg), calcium (200 to 755 mg/kg), magnesium (110 to 413 mg/kg), fibre (4.98 to 12.22 g/kg), dry matter (59 to 140 g/kg), nitrates (293 to 3,817 mg/kg) and the weight of leaf rosette (164 to 502 g). A significant influence of cultivar was found in the case of K, Na, and Ca content, as well as in dry matter and weight of leaf rosette. The growing season affected significantly all the evaluated substances, except for fibre. The year of cultivation affected all the evaluated parameters but Ca. It appears from the results that the contents of monitored substances were significantly influenced by cultivar as well as by growing season and year. Keywords: leaf lettuce (Lactuca sativa L. var. crispa), cultivars, nutrients Journal: Horticultural Science Pages: 99-106 Volume: 35 Issue: 3 Year: 2008 DOI: 10.17221/3/2008-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/3/2008-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200803-0002.txt Handle: RePEc:caa:jnlhor:v:35:y:2008:i:3:id:3-2008-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: R. Kapoor Author-Workplace-Name: Department of Biotechnology, University of Horticulture & Forestry, Solan, India Author-Name: S. Kumar Author-Workplace-Name: Department of Biotechnology, University of Horticulture & Forestry, Solan, India Author-Name: J. K. Kanwar Author-Workplace-Name: Department of Biotechnology, University of Horticulture & Forestry, Solan, India Title: Bulblet regeneration from ex vitro root explant in lily hybrids Abstract: The influence of growth regulators on in vitro bulblet formation from ex vitro roots was studied in asiatic and oriental hybrids of Lilium The root segments (3-4 mm) isolated from the middle zone of 2-3 cm ex vitro root were cultured on Murashige and Skoog (MS) medium containing 1 or 1.5 mg/dm3 naphthalene acetic acid (NAA) and/or benzyladenine (BA). Bulblets were not produced in the presence of NAA and BA alone. A significant increase in the per cent explants producing bulblets was observed with 1.5 mg/dm3 NAA and 1 mg/dm3 BA. Maximum number of bulblets and average fresh weight per bulblet was observed with 2 mg/dm3 NAA and 1.5 mg/dm3 BA after 90 days of culture. No differences were found among cultivars in bulblet regeneration of explant or bulblet number although more weighty bulblets occurred in cv. Apeldoorn. About 82% bulblet survival was recorded in coco peat after 30 days of transfer to pots. Keywords: lily hybrids, cvs. Alaska, Apeldoorn, Beartix, Siberia, Marco Polo, NAA, BA, IBA, in vitro, bulblet regeneration, ex vitro root, rooting, hardening Journal: Horticultural Science Pages: 107-112 Volume: 35 Issue: 3 Year: 2008 DOI: 10.17221/7/2008-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/7/2008-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200803-0003.txt Handle: RePEc:caa:jnlhor:v:35:y:2008:i:3:id:7-2008-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: E. Křístková Author-Workplace-Name: Department of Botany, Faculty of Science, Palacký University in Olomouc, Olomouc-Holice, Czech Republic Author-Name: I. Doležalová Author-Workplace-Name: Department of Botany, Faculty of Science, Palacký University in Olomouc, Olomouc-Holice, Czech Republic Author-Name: A. Lebeda Author-Workplace-Name: Department of Botany, Faculty of Science, Palacký University in Olomouc, Olomouc-Holice, Czech Republic Author-Name: V. Vinter Author-Workplace-Name: Department of Botany, Faculty of Science, Palacký University in Olomouc, Olomouc-Holice, Czech Republic Author-Name: A. Novotná Author-Workplace-Name: Department of Botany, Faculty of Science, Palacký University in Olomouc, Olomouc-Holice, Czech Republic Title: Description of morphological characters of lettuce (Lactuca sativa L.) genetic resources Abstract: Lettuce (Lactuca sativa) is the most important crop in the group of leafy vegetables. It is characterized by considerable morphological and genetic variation. The crop comprises seven main groups of cultivars (including oilseed lettuce) differing phenotypically; they are usually described as morphotypes. Lettuce breeding is primarilly focused on various morphological features and resistance against diseases and pests. The accurate description of lettuce germplasm provides basic information useful for lettuce breeders. The construction of a lettuce descriptor list has been stimulated by the international genebank community. This list consists of 55 descriptors with 15 elucidated by figures. It provides a tool for detailed characterization of and discrimination within the intraspecific variation of L. sativa, verification of old varieties, and identification of putative duplicates and gaps in germplasm collections. These descriptors, along with descriptors of wild Lactuca species, provide an efficient analytical tool for studying the complex morphological variability of this genus and relationships among the species. Keywords: biology, characterization, descriptors, gene bank collections, gene pool, germplasm conservation, morphotypes, origin, regeneration, resistance, taxonomy, variability Journal: Horticultural Science Pages: 113-129 Volume: 35 Issue: 3 Year: 2008 DOI: 10.17221/4/2008-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/4/2008-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200803-0004.txt Handle: RePEc:caa:jnlhor:v:35:y:2008:i:3:id:4-2008-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: H. Stavělíková Author-Workplace-Name: Department of Vegetables and Special Crops, Crop Research Institute, Prague-Ruzyně, Olomouc, Czech Republic Title: Morphological characteristics of garlic (Allium sativum L.) genetic resources collection - Information Abstract: Maintenance of garlic (Allium sativum L.) genetic resources has had a long tradition in the Czech Republic. The collection was founded in 1951 and its current holder is the Crop Research Institute, Department of Vegetables and Special Crops in Olomouc. The collection consists of 613 genotypes. The oldest genotype was acquired in 1954. The collection has been evaluated according to the Descriptor list for Allium spp. (IPGRI 2001). 17 characters of the Descriptor list were supplemented with 5 additional characters. The photos of all garlic genotypes were taken. The genotypes were divided into the three main morphological groups according to the ability to produce the scape: the first group with the scape (bolting) - 300 genotypes, the second part without the scape (non-bolting garlic) 205 genotypes and the third part presents semi-bolting garlic - 108 genotypes. The last morphological type has bulbils in the different parts of the pseudostem. Keywords: garlic, morphological characteristic, genetic resources, scape Journal: Horticultural Science Pages: 130-135 Volume: 35 Issue: 3 Year: 2008 DOI: 10.17221/661-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/661-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200803-0005.txt Handle: RePEc:caa:jnlhor:v:35:y:2008:i:3:id:661-HORTSCI