Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: Index of Volume 36 Journal: Horticultural Science Pages: I-II Volume: 36 Issue: 4 Year: 2009 DOI: 10.17221/2909-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/2909-HORTSCI.html File-Format: text/html Handle: RePEc:caa:jnlhor:v:36:y:2009:i:4:id:2909-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: P. Hanáček Author-Workplace-Name: Department of Plant Biology, Faculty of Agronomy, Mendel University of Agriculture and Forestry in Brno, Brno, Czech Republic Author-Name: T. Vyhnánek Author-Workplace-Name: Department of Plant Biology, Faculty of Agronomy, Mendel University of Agriculture and Forestry in Brno, Brno, Czech Republic Author-Name: M. Rohrer Author-Workplace-Name: Department of Plant Biology, Faculty of Agronomy, Mendel University of Agriculture and Forestry in Brno, Brno, Czech Republic Author-Name: J. Cieslarová Author-Workplace-Name: Department of Plant Biology, Faculty of Agronomy, Mendel University of Agriculture and Forestry in Brno, Brno, Czech Republic Author-Name: H. Stavělíková Author-Workplace-Name: Department of Vegetables and Special Crops, Crop Research Institute, Prague-Ruzyně, Workplace in Olomouc, Czech Republic Title: DNA polymorphism in genetic resources of red pepper using microsatellite markers Abstract: Genetic variability among 41 accessions of red pepper (Capsicum annuum L.) was assessed using eight microsatellite markers. Three of the microsatellite markers (Hpms 1-1, Hpms 1-168, and Hpms 1-274) had uniform spectra in all the analyzed plants. Two to eight alleles were detected for the remaining loci. In total, 28 alleles were detected, i.e. 3.5 alleles per one microsatellite locus on average. The highest number of different alleles was detected with Hpms 1-5 (8 alleles) and Hpms 2-21 primers (7 alleles). Molecular data were complemented with morphological measurements according to the descriptor list for the genus Capsicum. A dendrogram based on our genetic analysis suggests a high level of similarity between some of the accessions presumed to be distant and, at the same time, genetic variability between accessions of the same or similar name. These results show the possibility of duplicities in the current Czech collection of red pepper genetic resources. Keywords: pepper, genetic resources, microsatellites, SSRs, variability Journal: Horticultural Science Pages: 127-132 Volume: 36 Issue: 4 Year: 2009 DOI: 10.17221/7/2009-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/7/2009-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200904-0002.txt Handle: RePEc:caa:jnlhor:v:36:y:2009:i:4:id:7-2009-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: S. Z. Xin Author-Workplace-Name: Key Laboratory of Plant Nutrition, MOA, Key Laboratory of Plant-Soil Interactions, MOE, College of Resources and Environmental Sciences, China Agricultural University, Beijing, China P. R. Author-Name: Y. J. Song Author-Workplace-Name: Key Laboratory of Plant Nutrition, MOA, Key Laboratory of Plant-Soil Interactions, MOE, College of Resources and Environmental Sciences, China Agricultural University, Beijing, China P. R. Author-Name: C. Lv Author-Workplace-Name: Key Laboratory of Plant Nutrition, MOA, Key Laboratory of Plant-Soil Interactions, MOE, College of Resources and Environmental Sciences, China Agricultural University, Beijing, China P. R. Author-Name: Y. K. Rui Author-Workplace-Name: Key Laboratory of Plant Nutrition, MOA, Key Laboratory of Plant-Soil Interactions, MOE, College of Resources and Environmental Sciences, China Agricultural University, Beijing, China P. R. Author-Name: F. S. Zhang Author-Workplace-Name: Key Laboratory of Plant Nutrition, MOA, Key Laboratory of Plant-Soil Interactions, MOE, College of Resources and Environmental Sciences, China Agricultural University, Beijing, China P. R. Author-Name: W. Xu Author-Workplace-Name: Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China P. R. Author-Name: D. Wu Author-Workplace-Name: Key Laboratory of Plant Nutrition, MOA, Key Laboratory of Plant-Soil Interactions, MOE, College of Resources and Environmental Sciences, China Agricultural University, Beijing, China P. R. Author-Name: S. Wu Author-Workplace-Name: Key Laboratory of Plant Nutrition, MOA, Key Laboratory of Plant-Soil Interactions, MOE, College of Resources and Environmental Sciences, China Agricultural University, Beijing, China P. R. Author-Name: J. Zhong Author-Workplace-Name: Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China P. R. Author-Name: D. L. Chen Author-Workplace-Name: Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China P. R. Author-Name: Q. Chen Author-Workplace-Name: Key Laboratory of Plant Nutrition, MOA, Key Laboratory of Plant-Soil Interactions, MOE, College of Resources and Environmental Sciences, China Agricultural University, Beijing, China P. R. Author-Name: F. T. Peng Author-Workplace-Name: College of Horticulture Science and Engineering of Shandong Agricultural University, Taian, China P. R. Title: Application of synchrotron radiation X-ray fluorescence to investigate the distribution of mineral elements in different organs of greenhouse spinach Abstract: Consumption of vegetables is one of the most important ways of providing the body with mineral elements. However, it is not clear how mineral elements are distributed in different organs of vegetables, especially vegetables grown in greenhouses. The distribution of mineral elements in the root and leaves of greenhouse spinach was determined using synchrotron radiation XRF, the results indicated that the amount of various elements in different parts of the leaves, roots and stems were inequable. Generally, the content of the elements in the root base were slightly higher than in the other parts. The amounts in the root apices were much lower than those in the other parts while the amount of every element decreased gradually from the root base to the root apices. In stems, the amount of K, Ca, Fe, Ni, and Zn were higher in the base of the stem than in the top of stem while the amounts of Co and Cu were higher in the top of the stem than in the base of stem. From all the elements detected in the stem, Mn was at the lowest concentration. In leaves, Co and Zn were primarily accumulated around the main veins and the amount of Mn in the tip of the leaf was higher than in the other parts. In contrast, K, Ca, Ni, and Cu were higher in the center of the leaf. Keywords: spinach, distribution of mineral elements, synchrotron radiation XRF, leaves, roots, stem Journal: Horticultural Science Pages: 133-139 Volume: 36 Issue: 4 Year: 2009 DOI: 10.17221/18/2009-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/18/2009-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200904-0003.txt Handle: RePEc:caa:jnlhor:v:36:y:2009:i:4:id:18-2009-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: J. K. Kanwar Author-Workplace-Name: Department of Biotechnology, University of Horticulture & Forestry, Solan, India Author-Name: S. Kumar Author-Workplace-Name: Department of Biotechnology, University of Horticulture & Forestry, Solan, India Title: Influence of growth regulators and explants on shoot regeneration in carnation Abstract: The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in Dianthus caryophyllus L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots. Keywords: callus, carnation, growth regulators, hardening, Indios, in vitro, rooting, shoot regeneration Journal: Horticultural Science Pages: 140-146 Volume: 36 Issue: 4 Year: 2009 DOI: 10.17221/1/2009-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/1/2009-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200904-0004.txt Handle: RePEc:caa:jnlhor:v:36:y:2009:i:4:id:1-2009-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: F. Šrámek Author-Workplace-Name: Silva Tarouca Research Institute for Landscape and Ornamental Gardening, Průhonice, Czech Republic Author-Name: M. Dubský Author-Workplace-Name: Silva Tarouca Research Institute for Landscape and Ornamental Gardening, Průhonice, Czech Republic Title: Occurrence and correction of chlorosis in young petunia plants Abstract: : A glasshouse pot experiment tested the effects of 14 different combinations of substrate type, pH and nutrient treatments on the occurrence and severity of leaf chlorosis in a susceptible variety of petunia. Plants grown at optimal pH level (4.7) in peat substrate with low limestone dose were symptom-free even without added micronutrients. Severe chlorosis occurred in plants grown at high pH in peat substrate with high limestone (pH 6.7) and in peat-bark-compost (pH 6.2); it was associated with decreased Fe and Mn content in leaves. Regular application of nutrient solution with low concentration of Fe, Mn, and other micronutrients as EDTA chelates greatly reduced chlorosis in plants grown in peat-bark substrate and in peat-bark-compost, and it improved Fe uptake. An exception was peat substrate at high pH level and high limestone where chlorosis was only partially reduced by this treatment. Regular application of Fe, Mn, and other micronutrients as sulphates or citrates had no substantial effect; only application of three additional substrate drenches of 30 mg/l Mn from Mn-EDTA and 90 mg/l Fe from either Fe-EDTA or Fe-EDDHA substantially corrected chlorosis and increased foliar Fe and Mn in cases where plants were grown in high limestone peat substrate. Keywords: Petunia × atkinsiana, chlorosis, iron, manganese, iron chelates, Fe-EDTA, Fe-DTPA, Fe-EDDHA, Mn-EDTA Journal: Horticultural Science Pages: 147-153 Volume: 36 Issue: 4 Year: 2009 DOI: 10.17221/6/2009-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/6/2009-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200904-0005.txt Handle: RePEc:caa:jnlhor:v:36:y:2009:i:4:id:6-2009-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: P. Bulíř Author-Workplace-Name: Silva Tarouca Research Institute for Landscape and Ornamental Gardening, Průhonice, Czech Republic Title: Testing of Koch method applied for evaluation of ornamental trees in the Czech Republic Abstract: Koch method of ornamental trees evaluation is based on interest paid on costs invested in tree-growing up to their maturity stage minus deductions for age, defects and damage. The aim of this paper is to apply the above mentioned method in the economic conditions of the Czech Republic. The paper presents essential economic data generated in accordance with the technological model of tree growing at a permanent site. We also describe the mechanism of the so-called base tree price calculation as a basis for the subtraction of deductions derived from its current condition. The calculated base tree prices are illustratively compared to prices based on the to-date applied calculating techniques and the relevant regulation. We also refer to the method of base price adjustment based on the criterion of landscape tree value. The mechanism of price calculation applied for Koch method testing included two modifications. The first relates to due consideration of growth qualities when calculating development care costs; the other introduces landscaping value indicator as the only base price adjustment to achieve the resulting price, corresponding with the current tree condition. Keywords: Koch method, woody species evaluation, ornamental trees, tree-growing costs, development care, base price, resulting price, landscaping value Journal: Horticultural Science Pages: 154-161 Volume: 36 Issue: 4 Year: 2009 DOI: 10.17221/60/2008-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/60/2008-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200904-0006.txt Handle: RePEc:caa:jnlhor:v:36:y:2009:i:4:id:60-2008-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: J. Obdržálek Author-Workplace-Name: Silva Tarouca Research Institute for Landscape and Ornamental Gardening, Průhonice, Czech Republic Title: Cultivation of Cypripedium calceolus L. ex vitro seedlings in outdoor conditions: Short communication Abstract: The paper presents first positive results of the experiment with three-year outdoor cultivation of Cypripedium calceolus L. ex vitro seedlings in the Czech Republic. They were propagated in vitro from mature seeds of Carpathian provenance in a Prague private laboratory. In April 2006 after three months cool refrigerating at 4°C the seedlings were prepared for planting. The rhizomes with 4 to 12 roots and visible dormant buds were used. They were planted in two types of substrates: mixture AN on the basis of liadrain (burned clay pebbles) and mixture BN on the basis of granodiorite. Both mixtures were amended with perlite, pumice, sand, zeolite and dolomite lime powder. The mineral substrates proved to be stable and convenient for transfer and cultivation of ex vitro seedlings. Additional treatment with lignohumate in other two variants of the experiment did not improve the effect. The seedlings were grown outdoors on a shaded bed till the retracting leaves. They overwintered in a cold glasshouse with temperature close to zero from late November to March. The substrates did not visually influence the phase of sprouting, the phase of growth and retracting of the plants. At the end of the third growing season the yield of 4-year-old seedlings with two to four leaves ranged from 83% to 98% in four variants. In November 2008 seedlings were taken up from the mixes and were evaluated as bare root plants. The number of the living plants with visible new buds and the quality of root system were recorded and evaluated. The average length of roots in mixture A on the basis of liadrain and B on the basis of granodiorite was 14.5 cm and 12.1 cm, respectively. The rhizomes were planted into new mixtures immediately. These seedlings will be able to grow up to the blooming size during two or three seasons. Seven year-old potted seedlings of C. calceolus will be planted into gene resource area of the Silva Tarouca Research Institute for Landscape and Ornamental Gardening at Průhonice. Keywords: Cypripedium calceolus, ex vitro seedlings, cultivation substrates, wintering, sprouting, buds, roots, yield Journal: Horticultural Science Pages: 162-170 Volume: 36 Issue: 4 Year: 2009 DOI: 10.17221/4/2009-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/4/2009-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-200904-0007.txt Handle: RePEc:caa:jnlhor:v:36:y:2009:i:4:id:4-2009-HORTSCI