Template-Type: ReDIF-Article 1.0 Author-Name: A. Hauptmanová Author-Workplace-Name: Department of Virology, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: J. Polák Author-Workplace-Name: Department of Virology, Crop Research Institute, Prague-Ruzyně, Czech Republic Title: The elimination of Plum pox virus in plum cv. Bluefree and apricot cv. Hanita by chemotherapy of in vitro cultures Abstract: In vitro cultures of plum cv. Bluefree and apricot cv. Hanita infected with Plum pox virus (PPV) were used for the virus elimination by chemotherapy. Low ribavirin concentrations of 5 and 10 mg/l in Murashige-Skoog medium were applied in the treatment. Plum pox virus was completely eliminated by 5 mg/l of ribavirin in plum cv. Bluefree within twenty weeks and in apricot cv. Hanita in twelve weeks of the application. Plum pox virus was completely eliminated by 10 mg/l of ribavirin both in plum cv. Bluefree and apricot cv. Hanita within twelve weeks. The presence of PPV was not proved by RT-PCR. Clones of plum cv. Bluefree and apricot cv. Hanita were re-tested by RT-PCR one year after the termination of the ribavirin treatment and negative results confirmed the elimination of Plum pox virus. Keywords: sharka, plant virus, chemotherapy, ribavirin, apricot, plum Journal: Horticultural Science Pages: 49-53 Volume: 38 Issue: 2 Year: 2011 DOI: 10.17221/10/2010-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/10/2010-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201102-0001.txt Handle: RePEc:caa:jnlhor:v:38:y:2011:i:2:id:10-2010-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: M.F. Mahmoud Author-Workplace-Name: Plant Protection Department, Faculty of Agriculture, Suez-Canal University, Ismailia, Egypt Author-Name: M. Barta Author-Workplace-Name: Department of Dendrobiology, Arboretum Mlyňany SAS, Vieska nad Žitavou, Slovakia Title: Effect of gamma radiation on the male sterility and other quality parameters of peach fruit fly, Bactrocera zonata (Saunders) (Diptera: Tephritidae) Abstract: Bactrocera zonata, a serious pest of fruits in many parts of the world, has recently been recorded in Northern Africa. Even though it has not been introduced to the European continent yet, a strong emphasis is being placed on developing effective measures to suppress this pest and to prevent it from establishing in neighbouring European countries. The sterile insect technique is widely used in integrated programmes against tephritid fruit flies and, in this paper, quality parameters of irradiated B. zonata were evaluated for possible use of sterile insect technique within the management of this pest. Pupae were irradiated (60Co) 48 h before adult emergence (in an air atmosphere) with doses of 10, 30, 50, 70 or 90 Gy. While adult emergence and egg hatch decreased with increasing dose, no significant differences in female fecundity were found among doses. Exposure of pupae to 90 Gy resulted in a total sterility of eggs laid by non-treated females crossed with treated males. Only insignificant difference in the radiation effect on female fecundity was found. Moderate effects on sex ratio and size were recorded, as they decreased gradually by increasing doses. No considerable effect on flying capability was observed, but generally, the percentage of fliers decreased with increasing radiation doses. Fried's competitiveness values of treated males (30 and 70 Gy) suggest that irradiated males compete successfully with non-irradiated ones. Keywords: gamma radiation, tephritid fruit flies, sterile insect technique, sterility, mating competitiveness Journal: Horticultural Science Pages: 54-62 Volume: 38 Issue: 2 Year: 2011 DOI: 10.17221/88/2010-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/88/2010-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201102-0002.txt Handle: RePEc:caa:jnlhor:v:38:y:2011:i:2:id:88-2010-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: O. Rop Author-Workplace-Name: Department of Food Technology and Microbiology, Faculty of Technology, Tomas Bata University in Zlin, Zlin, Czech Republic Author-Name: V. Řezníček Author-Workplace-Name: Department of Breeding and Propagation of Horticultural Plants, Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic Author-Name: J. Mlček Author-Workplace-Name: Department of Food Technology and Microbiology, Faculty of Technology, Tomas Bata University in Zlin, Zlin, Czech Republic Author-Name: T. Juríková Author-Workplace-Name: Department of Natural and Informatics Sciences, Faculty of Central European Studies, Constantine the Philosopher University in Nitra, Nitra, Slovak Republic Author-Name: J. Balík Author-Workplace-Name: Department of Post-Harvest Technology of Horticultural Products, Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic Author-Name: J. Sochor Author-Workplace-Name: Department of Breeding and Propagation of Horticultural Plants, Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic Author-Name: D. Kramářová Author-Workplace-Name: Department of Food Biochemistry and Analysis, Faculty of Technology, Tomas Bata University in Zlin, Zlin, Czech Republic Title: Antioxidant and radical oxygen species scavenging activities of 12 cultivars of blue honeysuckle fruit Abstract: The fruit of blue honeysuckle (Lonicera caerulea L. var. kamtchatica (Sevast.) Pojark.) belongs to the fruit of great value because of itsunique chemical composition. The aim of our measurement of this fruit species was to select the cultivars which could be the most suitable food supplement in relation to strengthening human immunity system. The comparison of the fruit of 12 cultivars was made. The highest contents of polyphenolic substances were recorded in the cultivar Zolushka with the value of 9.03 g of gallic acid/kg fresh mass. In particular cultivars the DPPH (2,2-diphenyl-1-picrylhydrazyl) test was performed to determine antioxidant activity which ranged from 6.59-10.17 g of ascorbic acid equivalent/kg fresh mass. For comparison, scavenging activity of reactive oxygen species (superoxide anion, hydroxyl radical, and nitric oxide) was determined by using a 25% methanolic extract of fruit of particular cultivars. Antioxidant potential was also assessed using the rat liver slice model. The highest values of scavenging activity were found in the cultivars Zolushka, Goluboe vreteno, and Gerda. The cultivars Zolushka, Goluboe vreteno, and Gerda had also high values of flavonoids. The presented results may be used when popularizing this fruit species and also when studying the properties of new food and genetic resources. Keywords: Lonicera caerulea L., phenolics, antioxidant activity, lipid peroxidation, flavonoids Journal: Horticultural Science Pages: 63-70 Volume: 38 Issue: 2 Year: 2011 DOI: 10.17221/99/2010-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/99/2010-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201102-0003.txt Handle: RePEc:caa:jnlhor:v:38:y:2011:i:2:id:99-2010-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: M.D. Loureiro Author-Workplace-Name: Regional Government of Asturias Agrifoods R&D Service, SERIDA, Asturias, Spain Author-Name: P. Moreno-Sanz Author-Workplace-Name: Regional Government of Asturias Agrifoods R&D Service, SERIDA, Asturias, Spain Author-Name: B. Suárez Author-Workplace-Name: Regional Government of Asturias Agrifoods R&D Service, SERIDA, Asturias, Spain Title: Clonal preselection of grapevine cultivars of the appellation "Cangas Quality Wine" (Asturias, Spain) Abstract: The Asturian vineyard is composed mostly of autochthonous minority cultivars only present in the northwest of Spain. This vineyard is characterized by its antiquity, confusion as to the identity of certain cultivars, lack of certified plant material, and a great number of mixed cultivars in each individual vineyard. With the aim of restoring this viticulture after years of abandonment and improving the quality of its wines, old plants belonging to four red grapevine cultivars (Albarín tinto, Carrasquín, Verdejo tinto, and Mencía) presenting a good sanitary state and adequate yield were selected for clonal preselection. Agronomic and enological data were collected over three years. Those plants exhibiting above average values of probable alcohol content and yield for their respective vineyards were tested for viruses so as to discard unhealthy individuals. Six loci microsatellites were analysed to verify the identity of the selected plants. A final number of 62 clones belonging to these cultivars were selected for planting in a plot for their subsequent study under homogeneous conditions. Keywords: autochthonous cultivar, microsatellite, virus, Vitis vinifera L., wine quality Journal: Horticultural Science Pages: 71-80 Volume: 38 Issue: 2 Year: 2011 DOI: 10.17221/87/2010-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/87/2010-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201102-0004.txt Handle: RePEc:caa:jnlhor:v:38:y:2011:i:2:id:87-2010-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: M. Koudela Author-Workplace-Name: Department of Horticulture, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: F. Hnilička Author-Workplace-Name: Department of Botany and Plant Physiology, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: L. Svozilová Author-Workplace-Name: Department of Horticulture, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: J. Martinková Author-Workplace-Name: Department of Botany and Plant Physiology, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Title: Cauliflower qualities in two irrigation levels with the using of hydrophilic agent Abstract: The aim of this work was to evaluate the influence of a hydrophilic agent (Agrisorb), added to a substrate for seedling cultivation (3 g Agrisorb/l of substrate), on the weight of roots, weight, and height of the aboveground part, the number of leaves and root collar diameter of precultivated cauliflower seedlings (cultivar Chambord F1). Seedlings were planted under two different irrigation levels: optimal with available water capacity 80%, and reduced with 50%. The influence of Agrisorb on the following characteristics of cauliflower edible parts was evaluated after harvest: weight, diameter, percentage representation of marketable parts, market yield, content of ascorbic acid, nitrates and dry matter, and total antioxidative capacity value. The addition of Agrisorb significantly increased the weight of the aboveground parts by 17.3%, the root weight by 28.1%, and the number of leaves by 7.9%. After harvest, it was noted that Agrisorb significantly increased marketable yield (by 17.5%) and decreased total antioxidative capacity (by 19.9%) in the treatment with a reduced irrigation level. Keywords: water stress, seedlings, market yield, chemical composition Journal: Horticultural Science Pages: 81-85 Volume: 38 Issue: 2 Year: 2011 DOI: 10.17221/149/2010-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/149/2010-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201102-0005.txt Handle: RePEc:caa:jnlhor:v:38:y:2011:i:2:id:149-2010-HORTSCI