Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: INDEX OF VOLUME 38 Journal: Horticultural Science Pages: I-II Volume: 38 Issue: 4 Year: 2011 DOI: 10.17221/3333-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/3333-HORTSCI.html File-Format: text/html Handle: RePEc:caa:jnlhor:v:38:y:2011:i:4:id:3333-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: E. Chroboková Author-Workplace-Name: Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic Author-Name: J. Raddová Author-Workplace-Name: Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic Author-Name: M. Vachůn Author-Workplace-Name: Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic Author-Name: B. Krška Author-Workplace-Name: Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic Author-Name: M. Pidra Author-Workplace-Name: Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic Title: An analysis of apricot cultivars by random amplified polymorphic DNA and microsatellite primers Abstract: The random amplified polymorphic DNA (RAPD) technique and microsatellites were used to study the genetic diversity and to identify cultivars within a collection of 95 cultivars of Prunus armeniaca L. A dendrogram based on 13 RAPD primers and a dendrogram based on 9 microsatellite primers were prepared using the unweighted pair group method with average (UPGMA) group analysis. In both dendrograms, the cultivars were classified into five groups, according to their geographic origin: hybrids originated by hybridization among cultivars of European and Asian origin, European cultivars, American cultivars, Asian cultivars and interspecific hybrids. Eleven cultivars were not distinguished (9 cultivars with supposed relatedness to Velkopavlovická cv., 2 cvs Vynoslivyj and Vynoslivyj 21/1 that are assumed to be clones) using 9 microsatellite primers. The similarities and the differences revealed among incorporation of cultivars into groups were compared with the literature findings. The results of these analyses have a direct implication on the selection of new breeding progenitors at the Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic. Keywords: Prunus armeniaca L., microsatellites, RAPD primers, cultivar identification Journal: Horticultural Science Pages: 125-133 Volume: 38 Issue: 4 Year: 2011 DOI: 10.17221/68/2010-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/68/2010-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201104-0002.txt Handle: RePEc:caa:jnlhor:v:38:y:2011:i:4:id:68-2010-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: S. Bayazit Author-Workplace-Name: Department of Horticulture, Faculty of Agriculture, University of Mustafa Kemal, Antakya, Hatay, Turkey Author-Name: B. Imrak Author-Workplace-Name: Department of Horticulture, Faculty of Agriculture, University of Çukurova, Adana, Turkey Author-Name: A. Küden Author-Workplace-Name: Department of Horticulture, Faculty of Agriculture, University of Çukurova, Adana, Turkey Author-Name: M. Kemal Güngör Author-Workplace-Name: Minister of Agriculture and Village Affairs (TAGEM), Ankara, Turkey Title: RAPD analysis of genetic relatedness among selected quince (Cydonia oblonga Mill.) accessions from different parts of Turkey Abstract: Quince (Cydonia oblonga Mill.) is a minor fruit crop, which is primarily used for marmalade, jam, sauce and as rootstocks for pears. Different cultivated and local quince genotypes are grown in almost all parts of Turkey for fruit usage. In this study, randomly amplified polymorphic DNA (RAPD) technology was used to study the genetic relationships among 13 quince accessions selected from different parts of Turkey. Thirty decamer primers were used and 14 of them did not produce any polymorphism. The remaining 16 primers ranged in their amplification fragments between one (P-402, P-437, OPA 10, OPA 16, OPA 18 and OPA-19) and five (OPA-06 and OPA-07). The size of fragments varied from 100 to 1500 bp. Similarity values among the studied genotypes ranged between 0.483 and 0.925. The resulting dendrogram clustered into two groups (0.69 similarity value) based on evaluation of genetic similarities and differences. The results suggest that RAPD analysis could be used to distinguish and determine genetic variation among quince accessions. Also, the obtained clustering based on RAPD markers agreed to some extent with the geographical origin of the studied set of quince accessions. Keywords: quince, genetic resource, molecular markers, identification, DNA polymorphism Journal: Horticultural Science Pages: 134-141 Volume: 38 Issue: 4 Year: 2011 DOI: 10.17221/97/2011-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/97/2011-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201104-0003.txt Handle: RePEc:caa:jnlhor:v:38:y:2011:i:4:id:97-2011-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: A. Turhan Author-Workplace-Name: MustafakemalpaŞa Vocational School, Uludag University, MustafakemalpaŞa, Turkey Author-Name: N. Ozmen Author-Workplace-Name: MustafakemalpaŞa Vocational School, Uludag University, MustafakemalpaŞa, Turkey Author-Name: M.S. Serbeci Author-Workplace-Name: MustafakemalpaŞa Vocational School, Uludag University, MustafakemalpaŞa, Turkey Author-Name: V. Seniz Author-Workplace-Name: Department of Horticulture, Faculty of Agricultural, Uludag University, Gorukle, Turkey Title: Effects of grafting on different rootstocks on tomato fruit yield and quality Abstract: The aim of the study was to find effects of tomato grafting on another cultivar. The tomato cultivars used as scions were Yeni Talya, Swanson and Beril. Cultivars used as rootstocks were Beaufort and Arnold. Cleft grafting methods were applied. The following characteristics of grafted and nongrafted plants were recorded: fruit index, number of fruits/truss, fruit weight, fruit yield, dry matter, pH, concentration of soluble solids, titratable acidity, total sugar and lycopene and vitamin C. The results showed that fruit yield and fruit index, number of fruits/truss and fruit weights were improved by grafting. Fruit quality, measured in terms of dry matter, concentration of soluble solids, total sugar, and vitamin C content, was lower in the fruits of grafted plants than in nongrafted ones. No significant difference in lycopene and pH content was found. Titratable acid content was improved by grafting. A positive effect of grafting was recorded when Beaufort was used as rootstock. These results showed that grafting could be an advantageous alternative in tomato production. Keywords: tomato, cultivars, grafting, yield, fruit index, fruit weight, titratable acids Journal: Horticultural Science Pages: 142-149 Volume: 38 Issue: 4 Year: 2011 DOI: 10.17221/51/2011-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/51/2011-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201104-0004.txt Handle: RePEc:caa:jnlhor:v:38:y:2011:i:4:id:51-2011-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: B. Navrátilová Author-Workplace-Name: Faculty of Science, Palacký University in Olomouc, Olomouc, Czech Republic Author-Name: D. Skálová Author-Workplace-Name: Faculty of Science, Palacký University in Olomouc, Olomouc, Czech Republic Author-Name: V. Ondřej Author-Workplace-Name: Faculty of Science, Palacký University in Olomouc, Olomouc, Czech Republic Author-Name: M. Kitner Author-Workplace-Name: Faculty of Science, Palacký University in Olomouc, Olomouc, Czech Republic Author-Name: A. Lebeda Author-Workplace-Name: Faculty of Science, Palacký University in Olomouc, Olomouc, Czech Republic Title: Biotechnological methods utilized in Cucumis research - A review Abstract: Our biotechnological research on selected Cucumis species has encompassed interspecific hybridization via embryo-rescue, in vitro pollination, somatic hybridization and cytogenetics of protoplasts. Embryo-rescue and in vitro pollination are suitable in vitro techniques for production of hybrid embryos. These methods were tested and optimized for cucurbits. Protoplast culture is another valuable tool for biotechnological applications, such as somatic hybridization and genetic transformation. We study protoplast dedifferentiation not only as a biotechnological application in breeding systems, but mainly to describe mechanisms of obtaining totipotency. Protoplasts of cucurbits were studied cytogenetically to observe changes in nuclear architecture during protoplastization and regeneration and for comparison with the expression profile obtained using cDNA-AFLP techniques and reverse transcription for the specific genes involved. Keywords: Cucumis spp., expression profile, interspecific hybridization, in vitro biotechnological methods Journal: Horticultural Science Pages: 150-158 Volume: 38 Issue: 4 Year: 2011 DOI: 10.17221/143/2010-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/143/2010-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201104-0005.txt Handle: RePEc:caa:jnlhor:v:38:y:2011:i:4:id:143-2010-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: J. Sedlák Author-Workplace-Name: Research and Breeding Institute of Pomology Holovousy, Ltd., Hořice, Czech Republic Author-Name: F. Paprštein Author-Workplace-Name: Research and Breeding Institute of Pomology Holovousy, Ltd., Hořice, Czech Republic Title: Micropropagation of cranberry (Vaccinium macrocarpon) through shoot tip cultures: Short communication Abstract: The goal of this study was to determine an efficient micropropagation system for cranberry (Vaccinium macrocarpon Ait.). Cranberry cultivar Howes was successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. Anderson's rhododendron medium (AN), half-strength Murashige and Skoog medium (half-MS) and McCown woody plant medium (WPM) containing the cytokinin zeatin in concentrations 0.5, 1 or 2 mg/l were tested. Generally, the highest multiplication rate (2.7) was obtained for cv. Howes on AN medium with the concentration 1 mg/l of zeatin. The effects of indole-3-butyric acid (IBA), indole-3-acetic acid (IAA) and naphtalen acetic acid (NAA) on root induction were tested in WPM medium. The percentage of rooting was 100% for NAA, 86% for IBA and 81% for IAA. From the tested variants, auxin NAA also promoted the highest development of good quality roots (more than 5) per shoot without callus formation. Keywords: in vitro, multiplication, rooting, explant, Howes Journal: Horticultural Science Pages: 159-162 Volume: 38 Issue: 4 Year: 2011 DOI: 10.17221/115/2010-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/115/2010-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201104-0006.txt Handle: RePEc:caa:jnlhor:v:38:y:2011:i:4:id:115-2010-HORTSCI