Template-Type: ReDIF-Article 1.0 Author-Name: Science Horticultural Title: INDEX OF VOLUME 41 Journal: Horticultural Science Pages: I Volume: 41 Issue: 4 Year: 2014 DOI: 10.17221/7741-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/7741-HORTSCI.html File-Format: text/html Handle: RePEc:caa:jnlhor:v:41:y:2014:i:4:id:7741-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: K. Sharma Author-Workplace-Name: Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: P. Sedlák Author-Workplace-Name: Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: D. Zeka Author-Workplace-Name: Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: P. Vejl Author-Workplace-Name: Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: J. Soukup Author-Workplace-Name: Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Title: Allele-specific PCR detection of sweet cherry self-incompatibility alleles S3, S4 and S9 using consensus and allele-specific primers in the Czech Republic Abstract: Prunus avium species of the Rosaceae family exhibit gametophytic self-incompatibility. Determination of the self-incompatibility genotype of individuals is essential for genetic studies and the development of informed management strategies. The PCR-based detection of S-allele helps to promote and speed up traditional breeding activity and hence molecular analysis of the perspective genotypes has become more intensive in all cherry growing countries. The alleles S3, S4 and S9 from 34 accessions of Czech collections were determined using the polymerase chain reaction (PCR) method. Initially, DNA extracts were amplified with consensus primers that amplify across the first, second, or both introns of the S-ribonuclease gene which shows a considerable length polymorphism. The new allele specific primers were designed with the goal to overcome some occurring difficulties in the detection of expected alleles by previously published allele specific primers. S-alleles fragments of standard cultivars used in this study were PCR amplified, sequenced to validate the designed primers. The study demonstrates the advantage of newly designed primers application in testing of sweet cherry genotypes. Keywords: Prunus avium, S-alleles, allele-specific primers, consensus primers Journal: Horticultural Science Pages: 153-159 Volume: 41 Issue: 4 Year: 2014 DOI: 10.17221/89/2014-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/89/2014-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201404-0002.txt Handle: RePEc:caa:jnlhor:v:41:y:2014:i:4:id:89-2014-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: M. Szymajda Author-Workplace-Name: Fruit Breeding Department, Division of Pomology, Research Institute of Horticulture, Skierniewice, Poland Author-Name: E. Żurawicz Author-Workplace-Name: Fruit Breeding Department, Division of Pomology, Research Institute of Horticulture, Skierniewice, Poland Title: Seed genotypes for harvesting seeds in the production of generative rootstocks for peach cultivars Abstract: Six novel peach genotypes, designated BN-1, BN-3, BN-4, BN-7, BN-8 and BN-45, were assessed for usefulness as sources of seeds for production of generative rootstocks for peach cultivars. The genotypes BN-8 and BN-1 were the most intensely flowering, while the most abundantly and regularly fruiting genotype was BN-8, which also produced the smallest stones. The smallest fruits and the highest seed yields were produced by the genotypes BN-8 and BN-7. All of the genotypes under assessment produced seeds with higher germination capacities compared with the two control cultivars, Mandżurska and Siberian C, with the seeds of the genotypes BN-8, BN-4 and BN-3 exhibiting the most significantly elevated germination capacities. The most stones with two seeds were produced by the genotypes BN-45 and BN-3; however, produced a smaller fruit crop than did BN-8. Considering the characteristics assessed, the best as a seed source trees was genotype BN-8. Keywords: breeding, Prunus persica, winter hardiness, stratification, germination capacity Journal: Horticultural Science Pages: 160-166 Volume: 41 Issue: 4 Year: 2014 DOI: 10.17221/86/2014-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/86/2014-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201404-0003.txt Handle: RePEc:caa:jnlhor:v:41:y:2014:i:4:id:86-2014-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: H. Weissinger Author-Workplace-Name: Department of Crop Sciences, Division of Viticulture and Pomology, University of Natural Resources and Life Sciences, Vienna, Austria Author-Name: H. Flachowsky Author-Workplace-Name: Institute for Breeding Research on Fruit Crops, Federal Research Centre for Cultivated Plants, Julius Kuehn-Institute, Dresden, Germany Author-Name: A. Spornberger Author-Workplace-Name: Department of Crop Sciences, Division of Viticulture and Pomology, University of Natural Resources and Life Sciences, Vienna, Austria Title: New strawberry genotypes tested for organic production on a Verticillium-infested site Abstract: Nineteen new strawberry breeding clones, bred at the Institute for Breeding Research on Fruit Crops in Dresden-Pillnitz, Germany, were evaluated for their suitability for organic production at a Verticillium-infested site in Vienna, Austria. Soil cover, plant vitality, resistance to leaf spot diseases as well as flower damages by the strawberry blossom weevil (Anthonomus rubi) and by spring frost were recorded. In two consecutive years, marketable yield and fruits infested by Botrytis cinerea were assessed. Three breeding clones, P-7189, P-8043 and P-8071, were considered as suitable for organic strawberry production. P-7189 and P-8043 showed high tolerance to Verticillium wilt. The breeding clones P-8155 and P-8166 performed well under organic management but had very soft fruits that were not acceptable for commercial fruit production. The rest of the breeding clones were low-yielding, however, some of them could be of interest for home gardens due to their good flavour and high plant vitality. Keywords: tolerance, resistance, root diseases, breeding, fruit quality Journal: Horticultural Science Pages: 167-174 Volume: 41 Issue: 4 Year: 2014 DOI: 10.17221/105/2014-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/105/2014-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201404-0004.txt Handle: RePEc:caa:jnlhor:v:41:y:2014:i:4:id:105-2014-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: M. Carvalho Author-Workplace-Name: Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology. University of Trás-os-Montes and Alto Douro, Vila Real, Portugal Author-Name: M. Matos Author-Workplace-Name: Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology. University of Trás-os-Montes and Alto Douro, Vila Real, Portugal Author-Workplace-Name: Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal Author-Name: V. Carnide Author-Workplace-Name: Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology. University of Trás-os-Montes and Alto Douro, Vila Real, Portugal Author-Workplace-Name: Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal Title: Fingerprinting of Vaccinium corymbosum cultivars using DNA of fruits Abstract: In recent years the production and consumption Vaccinium corymbosum has increased. Highbush blueberry cultivars are divided into three types, northern, intermediate and southern. The traditional methods for classification of highbush blueberry cultivars using morphological and flavour traits are largely unsuccessful, due to environmental influences. The genetic similarity of ten highbush blueberry cultivars was evaluated using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers from fruits and leaves. The DNA concentrations obtained in fruits and leaves were very similar and the band profiles observed in the two tissues were analogous with both molecular markers. RAPD analysis generated 144 bands, of which 112 were polymorphic (77.8%) in fruits and 141 bands of which 118 were polymorphic (83.7%) in leaves. In fruits, ISSR analysis produced 151 bands of which 127 were polymorphic (84.1%) and in leaves it produced 148 bands with 127 being polymorphic (85.8%). Dendrogram and principal coordinates analysis (PCO) analysis using the both markers results were concordant and a clear division of the types of highbush blueberry cultivars (northern and southern) into two distinct groups was verified. Keywords: molecular markers, blueberry, fruits and leaves, characterization Journal: Horticultural Science Pages: 175-184 Volume: 41 Issue: 4 Year: 2014 DOI: 10.17221/21/2014-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/21/2014-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201404-0005.txt Handle: RePEc:caa:jnlhor:v:41:y:2014:i:4:id:21-2014-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: Y. SUN Author-Workplace-Name: College of Life Science, University of Chinese Academy of Sciences, Beijing, P. R. China Author-Workplace-Name: Sino-Danish Center for Education and Research, Beijing, P. R. China Author-Name: P.E. HOLM Author-Workplace-Name: Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Taastrup, Denmark Author-Workplace-Name: Sino-Danish Center for Education and Research, Beijing, P. R. China Author-Name: F. LIU Author-Workplace-Name: Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Taastrup, Denmark Author-Workplace-Name: Sino-Danish Center for Education and Research, Beijing, P. R. China Title: Alternate partial root-zone drying irrigation improves fruit quality in tomatoes Abstract: Alternate partial root-zone drying (PRD) irrigation and deficit irrigation (DI) are water-saving irrigation strategies. Here, comparative effects of PRD and DI on fruit quality of tomato (Solanum lycopersicum L.) were investigated. The results showed that the irrigation treatments had no effect on tomato yield but significantly affected several organic and mineral quality attributes of the fruits. Compared to DI, PRD significantly increased the fruit concentrations of Ca and Mg, and fruit juice concentrations of total soluble solid, glucose, fructose, citric and malic acid, P, K and Mg. It is concluded that PRD is better than DI in terms of improving fruit quality, and could be a promising management strategy for simultaneous increase of water use efficiency and fruit quality in tomatoes. Keywords: water deficit, minerals, organic acids, sugars Journal: Horticultural Science Pages: 185-191 Volume: 41 Issue: 4 Year: 2014 DOI: 10.17221/259/2013-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/259/2013-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201404-0006.txt Handle: RePEc:caa:jnlhor:v:41:y:2014:i:4:id:259-2013-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: C. Penella Author-Workplace-Name: Departamento de Horticultura, Instituto Valenciano de Investigaciones Agrarias, Valencia, Spain Author-Name: S.G. Nebauer Author-Workplace-Name: Departamento de Producción Vegetal, Universitat Politècnica de València, Valencia, Spain Author-Name: S. López-Galarza Author-Workplace-Name: Departamento de Producción Vegetal, Universitat Politècnica de València, Valencia, Spain Author-Name: A. San Bautista Author-Workplace-Name: Departamento de Producción Vegetal, Universitat Politècnica de València, Valencia, Spain Author-Name: A. Rodríguez-Burruezo Author-Workplace-Name: Instituto de Conservación y Mejora de la Agrodiversidad Valenciana, Ciudad Politécnica de la Innovación. Universitat Politècnica de València, Valencia, Spain Author-Name: A. Calatayud Author-Workplace-Name: Departamento de Horticultura, Instituto Valenciano de Investigaciones Agrarias, Valencia, Spain Title: Evaluation of some pepper genotypes as rootstocks in water stress conditions Abstract: Water stress is a major environmental factor that limits crop production and it is important to develop crop varieties with higher yield under water scarcity. Increased pepper tolerance to water stress through grafting onto robust rootstocks could be an optimal alternative in the context of environmentally friendly agriculture. Our work evaluated the behaviour of 18 pepper genotypes during vegetative and reproductive stages under water stress in order to select tolerant genotypes to be used as rootstocks for pepper cultivation. The pepper tolerance screening was based on photosynthetic parameters. The genotypes Atlante, C-40, Serrano, PI-152225, ECU-973, BOL-58 and NuMex Conquistador were revealed as the most tolerant genotypes to water stress because they maintained net photosynthetic rate levels under water stress conditions. The selected genotypes were validated as rootstocks on a pepper cultivar in terms of productivity under severe water stress. Plants grafted onto cvs Atlante, PI-152225 and ECU-973 showed higher marketable yields when compared with ungrafted cultivar. Keywords: Capsicum annuum, chlorophyll fluorescence, graft, photosynthesis, yield Journal: Horticultural Science Pages: 192-200 Volume: 41 Issue: 4 Year: 2014 DOI: 10.17221/163/2013-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/163/2013-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201404-0007.txt Handle: RePEc:caa:jnlhor:v:41:y:2014:i:4:id:163-2013-HORTSCI