Template-Type: ReDIF-Article 1.0 Author-Name: Qijing Zhang Author-Workplace-Name: Shenyang Agricultural University, Shenyang, China Author-Workplace-Name: Liaoning Institute of Pomology, Yingkou, China Author-Name: Tong Li Author-Workplace-Name: Shenyang Agricultural University, Shenyang, China Author-Name: Lijie Zhang Author-Workplace-Name: Shenyang Agricultural University, Shenyang, China Author-Name: Wenxuan Dong Author-Workplace-Name: Shenyang Agricultural University, Shenyang, China Author-Name: Aide Wang Author-Workplace-Name: Shenyang Agricultural University, Shenyang, China Title: Expression analysis of NAC genes during the growth and ripening of apples Abstract: Plant-specific NAC transcription factors (TFs) play crucial roles in various pathways related to the stress response. However, to date, little information regarding NAC gene regulation during fruit ripening is available for the apple (Malus domestica). Here, we report that 13 out of 182 MdNAC genes were differentially expressed during the stages of fruit growth and ripening. Sequence analysis indicates that these 13 MdNAC genes harbour distinct structures and potentially diverse functions. The expression of both MdNAC1a and MdNAC78 was repressed by ethylene and induced by 1-MCP during storage. MdNAC2, MdNAC26, MdNAC41, MdNAC57, MdNAC80, MdNAC91, MdNAC119 and MdNAC141 were up-regulated by ethylene and their transcription mirrored ethylene production rates during storage. MdNAC1, MdNAC16 and MdNAC32 did not respond to 1-MCP exposure. Additionally, the 13 MdNAC genes identified displayed differential tissue-specific expression patterns. These results suggest that NAC TFs play an important role in the regulation of apple development via both ethylene-dependent and -independent mechanisms. Keywords: Malus domestica, NAC TFs, ethylene, fruit development, storage Journal: Horticultural Science Pages: 1-10 Volume: 45 Issue: 1 Year: 2018 DOI: 10.17221/153/2016-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/153/2016-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201801-0001.txt Handle: RePEc:caa:jnlhor:v:45:y:2018:i:1:id:153-2016-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: Jan Blažek Author-Name: Lubor Zelený Author-Workplace-Name: Research and Breeding Institute of Pomology Holovousy Ltd., Holovousy, Czech Republic Author-Name: Jana Křelinová Author-Workplace-Name: Research and Breeding Institute of Pomology Holovousy Ltd., Holovousy, Czech Republic Title: Long term cropping and selected parameters of 15 apple tree cultivars Abstract: Fifteen apple cultivars registered in the Czech Republic were included in this comparative study. The most productive after eight years of cropping was 'Rucla', which had a mean yield of 50.9 t/ha. This cultivar was also the most tolerant to late spring frosts. In order of decreasing yields, it was followed by the cultivars 'Rubinstep', 'King Jonagold' and 'Šampion'. The lowest yield was found for the cultivar 'Fuji Nagafu' (23.7 t/ha) followed by 'Golden Delicious' and 'Rubinola'. The largest canopy volume in 2016 was achieved by 'Rubín' at a level of 2.74 m3, followed in decreasing order by 'Rubinola', 'King Jonagold' and 'Meteor'. According to the final tree canopy volume, the least vigorous was 'Selena' with a mean of 1.50 m3. The largest canopy of 2.74 m3 was exhibited by 'Rubín'. The 'Braeburn' cultivar exhibited the highest degree of spur bearing, followed by 'Lady Silvia' and 'Vysočina'. In contrast, the poorest spur patterns were observed in 'Rubín' and 'Fuji Nagafu'. 'Meteor' was characterised by the densest canopy, whereas the 'Rubín' cultivar was the least dense. Keywords: Malus domestica Borkh., tree vigour, yield efficiency, tree performance, spurring habit Journal: Horticultural Science Pages: 11-17 Volume: 45 Issue: 1 Year: 2018 DOI: 10.17221/31/2017-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/31/2017-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201801-0002.txt Handle: RePEc:caa:jnlhor:v:45:y:2018:i:1:id:31-2017-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: Paweł Bielicki Author-Workplace-Name: Experimental Nursery, Research Institute of Horticulture, Skierniewice, Poland Author-Name: Marcin Pąśko Title: Influence of selected Polish and American rootstocks on the growth and yield of 'Golden Delicious Reinders' apple trees Abstract: This study was conducted in the period 2010-2015 to assess the influence of rootstocks on the growth and fruiting of apple trees of the 'Golden Delicious Reinders' cultivar. The experiment was conducted in an experimental orchard in Dąbrowice, Poland. The experimental material consisted of the Polish rootstocks P 66, P 67, P 68 and P 16, and the following American rootstocks of the Cornell Geneva series resistant to fire blight: CG.11, CG.41, CG.013 and CG.202. The rootstocks M.9T337, M.26, P 14 and P 60 were used as the control combination. The best-yielding trees were those from the CG.11 rootstock, while those from P 14 and P 67 were the lowest-yielding. Trees from the latter rootstocks grew most vigorously and had the lowest productivity index. Keywords: cultivation, fruiting trees, fruit quality Journal: Horticultural Science Pages: 18-21 Volume: 45 Issue: 1 Year: 2018 DOI: 10.17221/23/2017-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/23/2017-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201801-0003.txt Handle: RePEc:caa:jnlhor:v:45:y:2018:i:1:id:23-2017-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: Senad Murtic Author-Workplace-Name: Department of Agricultural Botany and Plant Physiology, Faculty of Agriculture and Food Sciences, University of Sarajevo, Sarajevo, Bosnia and Herzegovina Author-Name: Rodoljub Oljaca Author-Workplace-Name: Department of Soil Science, Plant Physiology and Nutrition, Faculty of Agriculture, University of Banja Luka, Banja Luka, Bosnia and Herzgovina Author-Name: Ivana Koleska Author-Workplace-Name: Department of Soil Science, Plant Physiology and Nutrition, Faculty of Agriculture, University of Banja Luka, Banja Luka, Bosnia and Herzgovina Author-Name: Lutvija Karic Author-Workplace-Name: Department of Vegetable Crops, Faculty of Agriculture and Food Sciences, University of Sarajevo, Sarajevo, Bosnia and Herzegovina Author-Name: Vida Todorovic Author-Workplace-Name: Department of Vegetable Crops, Faculty of Agriculture, University of Banja Luka, Banja Luka, Bosnia and Herzgovina Title: Response of cherry tomato seedlings to liquid fertiliser application under water stress Abstract: The aim of this study was to examine the impact of different liquid fertilisers on selected physiological parameters in order to evaluate the drought tolerance of cherry tomato seedlings. The following physiological parameters were investigated: total phenolic and flavonoid content, total antioxidant capacity and proline content of leaf extracts. Total phenolic and flavonoid content were determined using the Folin-Ciocalteu and aluminium chloride colorimetric methods, respectively. The ferric-reducing/antioxidant power (FRAP assay) was used to measure the total antioxidant capacity, while proline content was evaluated according to the method of Bates. The contents of proline, total phenolics and flavonoids were significantly higher in the leaves of cherry tomato seedlings exposed to water stress, which suggests that the higher synthesis of these substances by plants represents an important defence mechanism of drought tolerance. The results also indicate that the application of all the used fertilisers in accordance with the manufacturer's instructions can significantly increase the content of phenol compounds and total antioxidant capacity of plants under normal growth conditions, thus improving survival under subsequent stress. Keywords: phenolics, proline, drought, osmolytes, antioxidants Journal: Horticultural Science Pages: 22-28 Volume: 45 Issue: 1 Year: 2018 DOI: 10.17221/17/2017-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/17/2017-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201801-0004.txt Handle: RePEc:caa:jnlhor:v:45:y:2018:i:1:id:17-2017-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: Nicole Roberta Giuggioli Author-Workplace-Name: Department of Agricultural, Forest and Food Sciences, University of Torino, Torino, Italy Author-Name: Rosanela Briano Author-Workplace-Name: Department of Agricultural, Forest and Food Sciences, University of Torino, Torino, Italy Author-Name: Priscila Alvariza Author-Workplace-Name: Departmento de Fitotecnica, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, F Pelotas, Brasil Author-Name: Cristiana Peano Author-Workplace-Name: Department of Agricultural, Forest and Food Sciences, University of Torino, Torino, Italy Title: Preliminary evaluation of day-neutral strawberry cultivars cultivated in Italy using a qualitative integrated approach Abstract: In this study the texture profile, total soluble solids (TSS) and the fruit skin colour of four day-neutral strawberry cultivars cultivated in Italy ('Anabelle', 'Murano*', 'Portola*' and 'Triumph') was evaluated at four harvest times. The interrelationships of the analysed parameters were determined using principal component analysis in order to suggest the best commercial scenario (local market, supermarket retailer or export) for each cultivar. Data analysis related to texture and TSS contents revealed that 'Anabelle', 'Portola*' and 'Murano*' displayed unique characteristics, and that 'Triumph' showed lower values for these parameters. Due to its low hardness and cohesiveness values 'Anabelle' could be suited to a short supply chain; 'Portola*', meanwhile, might meet the requirements of a long supply chain, due to its high hardness and low TSS (P £ 0.05); 'Murano*' could be distinguished from the other cultivars by its high luminosity (ranging from 41.46-46.21 L*); while 'Triumph', due to its darkness (36.83-37.13 L*), could be suitable for a local/farmers' market. Keywords: colour, Fragaria × ananassa, market, quality index, texture Journal: Horticultural Science Pages: 29-36 Volume: 45 Issue: 1 Year: 2018 DOI: 10.17221/106/2016-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/106/2016-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201801-0005.txt Handle: RePEc:caa:jnlhor:v:45:y:2018:i:1:id:106-2016-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: Filippo Ferlito Author-Workplace-Name: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di Ricerca Olivicoltura, Frutticoltura e Agrumicoltura, Acireale (CT), Italy Author-Name: Elisabetta Nicolosi Author-Workplace-Name: Dipartimento di Agricoltura, Alimentazione e Ambiente, University of Catania, Catania, Italy Author-Name: Stefano La Malfa Author-Workplace-Name: Dipartimento di Agricoltura, Alimentazione e Ambiente, University of Catania, Catania, Italy Author-Name: Antonio Cicala Author-Workplace-Name: Dipartimento di Agricoltura, Alimentazione e Ambiente, University of Catania, Catania, Italy Author-Name: Alessandra Gentile Author-Workplace-Name: Dipartimento di Agricoltura, Alimentazione e Ambiente, University of Catania, Catania, Italy Title: First characterisation of minor and neglected Vitis vinifera L. cultivars from Mount Etna Abstract: Eight minor and neglected cultivars of Vitis vinifera L. were characterised according to their ampelographic and agronomic traits and discriminated by molecular analysis with SSR markers. These results are the first complete ampelographic description of these minor and neglected cultivars from the Mount Etna region. The results of this study reveal high morphological diversity of ancient grapevines growing in this region. SSR markers enabled us to discriminate the cultivars and revealed the genetic divergence between them and several autochthonous cultivars. Our efforts could contribute to a better knowledge of grape biodiversity based on morphological and molecular data and could be useful for the development of a reliable germplasm conservation strategy. Keywords: Sicily, grapevine, biometric, SSR, identification Journal: Horticultural Science Pages: 37-46 Volume: 45 Issue: 1 Year: 2018 DOI: 10.17221/198/2016-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/198/2016-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201801-0006.txt Handle: RePEc:caa:jnlhor:v:45:y:2018:i:1:id:198-2016-HORTSCI Template-Type: ReDIF-Article 1.0 Author-Name: Andrea Muráriková Author-Name: Jarmila Neugebauerová Author-Workplace-Name: Department of Vegetable growing and Floriculture, Faculty of Horticulture, Mendel University in Brno, Brno, Czech Republic Title: Seasonal variation of ascorbic acid and nitrate levels in selected basil (Ocimum basilicum L.) varieties Abstract: The aim of this study was to determine the levels of ascorbic acid and total nitrates in the fresh aerial parts of different varieties of basil (Ocimum basilicum L.) in different growing seasons. The ascorbate-nitrate index (IAN) is used to determine the safety of basil for consumers. Seven basil varieties ('Ohře', 'Lettuce Leaf', 'Purple Opaal', 'Dark Green', 'Mammolo Genovese', 'Mánes' and 'Red Rubin') were cultivated as pot cultures in greenhouses during the period 2015-2016. High-performance liquid chromatography (HPLC) was used to determine the ascorbic acid content, which ranged from 34.3 to 222.0 mg/kg f. w. An ion-selective electrode was employed to determine the content of nitrates, which ranged from 237.7 to 4280.6 mg/kg f. w. The ascorbate-nitrate index reached values of between 0.016 and 0.629, which are risky-to-moderately safe ascorbate-nitrate ratios. However, the occasional use of small amounts of basil as a culinary herb is not risky for consumers. Furthermore, significant differences in the content of ascorbic acid and nitrates were found among the cultivated samples with respect to variety, year and growing season. Keywords: culinary herbs, greenhouse production, pot culture, ascorbate-nitrate index Journal: Horticultural Science Pages: 47-52 Volume: 45 Issue: 1 Year: 2018 DOI: 10.17221/216/2016-HORTSCI File-URL: http://hortsci.agriculturejournals.cz/doi/10.17221/216/2016-HORTSCI.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/hor-201801-0007.txt Handle: RePEc:caa:jnlhor:v:45:y:2018:i:1:id:216-2016-HORTSCI