Template-Type: ReDIF-Article 1.0 Author-Name: Yedil Aishuk Author-Workplace-Name: Kokshetau University named Shokan Ualikhanov, Kokshetau, Republic of Kazakhstan Author-Name: Dani Sarsekova Author-Workplace-Name: Kazakh National Agrarian Research University, Almaty, Republic of Kazakhstan Title: Microclonal propagation of Cotoneaster melanocarpus as a tool for ecosystem restoration and resilience in Kazakhstan Abstract: Degradation of steppe ecosystems in Kazakhstan requires propagation methods for native woody species. Seed propagation of Cotoneaster melanocarpus Fisch. ex Blytt is slow and irregular owing to dormancy. This study evaluated the effects of 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA) on in vitro shoot regeneration and multiplication. Overall, 200 apical and nodal explants from 40 visually healthy, naturally growing donor shrubs were cultured on Murashige and Skoog (MS) medium with five BAP/IBA treatments (T0-T4). Regeneration frequency, shoot number, shoot length, callus formation, and shoot-development patterns were summarised descriptively after 30 days. T3 produced the highest response (2.0 mg.L-1 BAP and 0.5 mg.L-1 IBA): 37/40 explants regenerated (92.5%), yielding 5.2 shoots per explant and 6.3 cm mean shoot length. In T4 (3.0 mg.L-1 BAP and 1.0 mg.L-1 IBA), 24/40 explants regenerated (60.0%), with visible callus and apical abnormalities. Shoots in T1-T3 developed predominantly without visible intervening callus, although histology was not performed. Thus, T3 was the most favourable treatment tested for shoot multiplication. The findings are limited to shoot multiplication; rooting, acclimatisation, genetic fidelity, stress tolerance, and field performance require evaluation before restoration use. Keywords: axillary bud proliferation, culture-medium optimisation, cytokinin-, auxin balance, morphogenic response, Rosaceae Journal: Journal of Forest Science Pages: 422-429 Volume: 72 Issue: 8 Year: 2026 DOI: 10.17221/41/2026-JFS File-URL: http://jfs.agriculturejournals.cz/doi/10.17221/41/2026-JFS.html File-Format: text/html File-URL: http://jfs.agriculturejournals.cz/doi/10.17221/41/2026-JFS.pdf File-Format: Application/pdf X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/jfs-202608-0001.txt Handle: RePEc:caa:jnljfs:v:72:y:2026:i:8:id:41-2026-JFS Template-Type: ReDIF-Article 1.0 Author-Name: Lucia Kružlicová Author-Workplace-Name: Department of Forest Economics and Policy, Faculty of Forestry, Technical University in Zvolen, Zvolen, Slovakia Author-Name: Daniel Halaj Author-Workplace-Name: Department of Forest Economics and Policy, Faculty of Forestry, Technical University in Zvolen, Zvolen, Slovakia Title: Strategic asymmetry in a contractor-mediated forest biomass supply chain: Evidence from Central Slovakia Abstract: A reliable forest-biomass supply requires alignment among resource owners, processing contractors and energy users. This study examined governance and actor-specific strategic positions in a central Slovak chain comprising the state forest enterprise LESY SR, the contractor WorldWood and the Zvolen district-heating plant. Four management informants were interviewed using pre-tested semi-structured guides; the resulting factors were rated for impact (1-5) and importance (1-3), triangulated with documentary and operational evidence, and converted into weighted SWOT-TOWS coordinates. LESY SR (x = 16; y = -61) and the heating plant (x = 36; y = -26) occupied the strength-threat quadrant. WorldWood had a strong internal position (x = 57), but its external coordinate was boundary-sensitive: y ranged from -3 to 3 across the permitted importance weights for one corrected item. The chain was contractor-mediated and weakly integrated; principal system-level risks included seasonal mismatch, short-horizon tendering, price volatility, fuel-quality variability, and transport and storage costs. The contribution is to demonstrate that strategic asymmetry, rather than a lack of technical capacity, constrains coordination. Rolling volume forecasts, risk-sharing framework contracts, quality-based payment, regional inventory planning and interoperable traceability records are recommended. The study provides a replicable actor-level diagnostic for early-stage biomass-chain evidence. Keywords: district heating, expert weighting, seasonal inventory, sustainability certification, tender procurement, TOWS analysis Journal: Journal of Forest Science Pages: 430-439 Volume: 72 Issue: 8 Year: 2026 DOI: 10.17221/55/2026-JFS File-URL: http://jfs.agriculturejournals.cz/doi/10.17221/55/2026-JFS.html File-Format: text/html File-URL: http://jfs.agriculturejournals.cz/doi/10.17221/55/2026-JFS.pdf File-Format: Application/pdf X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/jfs-202608-0002.txt Handle: RePEc:caa:jnljfs:v:72:y:2026:i:8:id:55-2026-JFS Template-Type: ReDIF-Article 1.0 Author-Name: Jialiang Yang Author-Workplace-Name: School of Computer Science and Engineering, University of New South Wales, Sydney, Australia Author-Name: Chun Qin Author-Workplace-Name: Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, China Title: Forest digital twin via big data integration: Current advances and future perspectives Abstract: Forest ecosystems face increasing pressures from climate change, biodiversity loss, and human activities, necessitating innovative tools for management and prediction. Forest digital twin is emerging as a transformative tool for monitoring, simulating, and managing forest ecosystems. By integrating multi-source big data - satellite imagery, airborne and terrestrial LiDAR, Internet of Things (IoT) sensors, and process-based ecological models - the forest digital twin can provide dynamic, high-resolution representations of forest structure and function. This review integrates and analyses recent advances reported across 56 scholarly works and finds several gaps in current research, including limited real-time integration of IoT and remote-sensing data, weak interoperability between modelling platforms, and insufficient multi-scale ecological representation. This review addresses these shortcomings by synthesising the technological, ecological, and computational components required for a functional forest digital twin. Overall, the review provides a coherent conceptual framework, identifies critical research priorities, and demonstrates how digital twin technologies can transform forest monitoring and management through adaptive, data-driven decision support. Keywords: Internet of Things (IoT), forest fire, forest management, remote sensing Journal: Journal of Forest Science Pages: 383-399 Volume: 72 Issue: 8 Year: 2026 DOI: 10.17221/47/2026-JFS File-URL: http://jfs.agriculturejournals.cz/doi/10.17221/47/2026-JFS.html File-Format: text/html File-URL: http://jfs.agriculturejournals.cz/doi/10.17221/47/2026-JFS.pdf File-Format: Application/pdf X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/jfs-202608-0003.txt Handle: RePEc:caa:jnljfs:v:72:y:2026:i:8:id:47-2026-JFS Template-Type: ReDIF-Article 1.0 Author-Name: Jinqiu Li Author-Workplace-Name: College of Biological Science and Food Engineering, Forest Resources Exploitation and Utilisation Engineering Research Centre for Grand Health of Yunnan Provincial Universities, Southwest Forestry University, Kunming, China Author-Name: Xiaofei Shi Author-Workplace-Name: School of Ethnic Medicine, Yunnan Minzu University, Kunming, China Author-Name: Jing Li Author-Workplace-Name: College of Biological Science and Food Engineering, Forest Resources Exploitation and Utilisation Engineering Research Centre for Grand Health of Yunnan Provincial Universities, Southwest Forestry University, Kunming, China Title: Laboratory synthesis of ectomycorrhizae between ectomycorrhizal fungi and Pinus hosts Abstract: Ectomycorrhizal (ECM) fungi are integral components of forest ecosystems, playing crucial roles in ecological succession and biodiversity maintenance. Fossil evidence suggests that Pinaceae represent one of the earliest ectomycorrhiza-forming host lineages, and that the symbiotic relationship between Pinus species and ECM fungi is characterised by strong host specificity. Ectomycorrhizal fungi functionally substitute for root hairs, expanding the absorptive surface area of host root systems, thereby enhancing nutrient uptake, improving stress tolerance, and boosting the survival of outplanted seedlings. In recent years, ECM has garnered widespread attention and been widely applied in fields including forest seedling cultivation, stress-site afforestation, plant disease management, and edible mycorrhizal fungi utilisation. The ecological and practical importance of ectomycorrhizae has driven current research to focus on mechanisms of symbiosis establishment, ecological functions, and application strategies. However, progress in these research areas is highly dependent on the improvement and optimisation of experimental systems and methodologies for the synthesis of ectomycorrhizae. This review focuses on common methods for the laboratory synthesis of ectomycorrhizae (Figure 1), systematically summarising the fundamental principles, operational procedures, advantages, and limitations of key techniques, including two classic culture systems (sterile growth pouch system and agar plate symbiosis system) and two main inoculation approaches (mycelial plug inoculation and spore inoculation). It aims to provide a methodological reference for fundamental research on ectomycorrhizae and for their application in Pinus seedling cultivation and ecological restoration. Keywords: ecological restoration, inoculation methods, host specificity, nutrient uptake, symbiosis establishment Journal: Journal of Forest Science Pages: 400-421 Volume: 72 Issue: 8 Year: 2026 DOI: 10.17221/52/2026-JFS File-URL: http://jfs.agriculturejournals.cz/doi/10.17221/52/2026-JFS.html File-Format: text/html File-URL: http://jfs.agriculturejournals.cz/doi/10.17221/52/2026-JFS.pdf File-Format: Application/pdf X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/jfs-202608-0004.txt Handle: RePEc:caa:jnljfs:v:72:y:2026:i:8:id:52-2026-JFS