Template-Type: ReDIF-Article 1.0 Author-Name: Miroslav Oborník Author-Name: Richard Stouthamer Author-Name: Ellis Meekes Author-Name: Menno Schilthuittzen Title: Molecular characterization and phylogeny of the entomopathogenic fungus Abstract: We characterized 23 isolates of the entomopathogenic fungus Aschersonia spp. from Mexico, Brazil, Guyana, Trinidad, Venezuela, Columbia, Florida, Malaysia, Thailand, Japan, Philippines, Java and South India using RAPD markers. The data were used to compute the genetic variability and to reconstruct the phylogeny of the genus Aschersonia. Relative genetic distances varied from 0.018 (between isolates Aa2 and Ap2) to 0.445 (between isolates A1 and At1). In the constructed phylogenetic tree, isolates were clustered according to their geographical origin. We determined partial 26S ribosomal DNA sequences of five Aschersonia isolates (A28, A31, Ai1a, Ai2b - Aschersonia spp.; and Ap1- Aschersonia placenta) and used them for phylogenetic analysis. Three of the tested isolates were not distinguishable. The tree constructed indicated that isolates Ai1a and Ai2b belong to species distinct from A. placenta and A. aleyrodis. Keywords: Aschersonia, biological control, genetic variability, RAPD, phylogeny, rDNA Journal: Plant Protection Science Pages: 1-9 Volume: 35 Issue: 1 Year: 1999 DOI: 10.17221/9664-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/9664-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-199901-0001.txt Handle: RePEc:caa:jnlpps:v:35:y:1999:i:1:id:9664-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Aleš Svatoš Author-Name: Blanka Kalinová Author-Name: Michal Hoskovec Author-Name: Jiří Kindl Author-Name: Ivan Hrdý Title: Chemical communication in horse-chestnut leafminer Cameraria ohridella Deschka & Dimić. Abstract: Females of the horse-chestnut leafminer Cameraria ohridella Deschka & Dimić (Lepidoptera: Gracillariidae) produce a highly attractive sex pheromone in the early photophase. The pheromone, luring conspecific males into Delta traps baited with females, is produced in the female abdominal tip (ca 10-50 pg per calling female) and the active components can be extracted with hexane. Both dissected tips and their hexane extracts show high attractiveness for C. ohridella males in a wind tunnel behavioural bioassay. According to electrophysiologic recordings the male antennae are notably sensitive to both dissected female abdomens and extracts from them. The strong attractiveness of the pheromone may be useful in control of this pest. Keywords: Cameraria ohridella, Lepidoptera, Gracillariidae, chemical communication, EAG, wind tunnel, field trapping Journal: Plant Protection Science Pages: 10-13 Volume: 35 Issue: 1 Year: 1999 DOI: 10.17221/9665-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/9665-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-199901-0002.txt Handle: RePEc:caa:jnlpps:v:35:y:1999:i:1:id:9665-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Eva Ždarková Author-Name: Pavel Horák Title: Development of Cheyletus eruditus (Schrank) (Acarina: Cheyletidae) at low temperature Abstract: Development of the predatory mite C. eruditus was examined at 12, 14 and 25°C and 75% r.h. 12°C is the low temperature threshold for development of this species where development lasts 164 days, compared with 47 days of its prey Acarus siro under the same conditions. Since repressive biological control cannot be successful under those circumstances, the predators can only be used preventively in empty stores. Keywords: Cheyletus eruditus, biological control, development, low temperature Journal: Plant Protection Science Pages: 14-16 Volume: 35 Issue: 1 Year: 1999 DOI: 10.17221/9666-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/9666-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-199901-0003.txt Handle: RePEc:caa:jnlpps:v:35:y:1999:i:1:id:9666-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Peter Bokor Author-Name: Ľudovít Cagáň Title: Occurrence and bionomics of Eriborus terebrans (Gravenhorst) (Hymenoptera: Ichneumonidae), a parasitoid of the European corn borer, Ostrinia nubilalis Hbn. (Lepidoptera: Pyralidae), in Central Europe Abstract: Eriborus terebrans (Gravenhorst), the parasitoid of the European corn borer, Ostrinia nubilalis Hbn., was studied at four locations in central Europe during 1993-1995. Regular parasitism of O. nubilalis was found only at Blatnice in Moravia (eastern part of Czech Republic). At this location, the parasitism was 2.22% in 1993, 0.47% in 1994 and 0.06% in 1995. In 1994 and 1995, low parasitism (0.56 and 0.12% ,respectively) was found at Kráľovský Chlmec (eastern Slovakia). The records are the first from Czech Republic and Slovakia. The parasitoid was not found at Nitra (south-western Slovakia) and Wroclaw (south-western Poland). The first cocoons of E. terebrans developed in the first half of June. Parasitoid adults emerged from mid June to mid July. Results showed complete coincidence between bionomics of E. terebrans and bionomics of its host O. nubilalis. Keywords: Ostrinia nubilalis, Eriborus terebrans, parasitism, parasitoid, bionomics Journal: Plant Protection Science Pages: 17-22 Volume: 35 Issue: 1 Year: 1999 DOI: 10.17221/9667-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/9667-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-199901-0004.txt Handle: RePEc:caa:jnlpps:v:35:y:1999:i:1:id:9667-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Václav Stejskal Author-Name: Jan Zuska Author-Name: Petr Werner Author-Name: Zuzana Kučerová Title: Survival over the winter of Rhyzopertha dominica F. (Coleoptera: Bostrichidae) in hot spots caused by improper grain storage technology: the first record in the Czech Republic Abstract: The survival of dense populations of Rhyzopertha dominica in stored grain hot spots in the Czech Republic during winter months was documented for the first time. Improper storage of grain causes subsurface hot zones, which seem to provide conditions for pest overwintering and become the source of subsequent cross infestations. Keywords: Rhyzopertha dominica F. (Coleoptera: Bostrichidae), stored grain technology, low and high temperature, geographical range extension Journal: Plant Protection Science Pages: 23-25 Volume: 35 Issue: 1 Year: 1999 DOI: 10.17221/9668-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/9668-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-199901-0005.txt Handle: RePEc:caa:jnlpps:v:35:y:1999:i:1:id:9668-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Antonín Pospisil Author-Name: Josef Benada Author-Name: Ivana Polisenská Title: Variability of resistance to common bunt of wheat Abstract: During 1997-1998, the reaction of the collections of winter and spring wheat varieties to Tilletia caries was determined. In both years the most diseased winter varieties were Simona and Sparta, the least diseased were Samara and Ilona. High variability in the level of infection was observed in some varieties (VIada, Estica, Regina, Vega). No winter wheat variety was completely resistant. The most diseased spring wheat variety was Alexandria in both years, while Grandur showed no infection. High variability in the level of infection was observed in the special trials with cv. Ina and Contra. In trials of the efficacy of seed dressing against T. caries and T. laevis there was variability only in the controls, but not in dressed variants. Keywords: Tilletia caries, 1illetia laevis, spring wheat, winter wheat, seed dressings Journal: Plant Protection Science Pages: 26-29 Volume: 35 Issue: 1 Year: 1999 DOI: 10.17221/9670-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/9670-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-199901-0006.txt Handle: RePEc:caa:jnlpps:v:35:y:1999:i:1:id:9670-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Jiban Kumar Kundu Author-Name: Pavel Ryšánek Title: Study of semipersistent transmission of beet yellows virus by the green peach aphid, Myzus persicae Sulz. Abstract: The optimal conditions for beet yellows virus (BYV) acquisition and transmission by Myzus persicae Sulz. were studied. Maximum recorded retention of BYV in aphids was 48 h. Preacquisition starvation of aphids influenced positively transmission efficiency if the acquisition access feeding period was short (30 min to 2 h), but after a longer such period it was without influence. The efficiency of transmission also depended on the acquisition access feeding period. Maximum transmission efficiency of starved and nonstarved aphids occurred after 2 h (75%) and 4-6 h (65%) of acquisition, respectively. Keywords: beet yellows virus, Myzus persicae, transmission efficiency, preacquisition starvation Journal: Plant Protection Science Pages: 30-32 Volume: 35 Issue: 1 Year: 1999 DOI: 10.17221/9669-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/9669-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-199901-0007.txt Handle: RePEc:caa:jnlpps:v:35:y:1999:i:1:id:9669-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Darina Kubelková Author-Name: Josef Špak Title: Virus diseases of poppy (Papaver somniferum L.) and some other species of the Papaveraceae family - a review Abstract: Opium poppy (Papaver somniferum L.) is described in the literature as a natural host of turnip mosaic virus, bean yellow mosaic virus, beet yellows virus and beet mosaic virus, and experimental host of plum pox virus. P. orientale L., a natural host of beet curly top virus, was successfully infected with turnip mosaic virus and cucumber mosaic virus, and P. dubium L. with turnip mosaic virus. P. rhoeas L. is a natural host of turnip mosaic virus, and artificial host of beet yellows, plum pox and cucumber mosaic viruses. P. nudicaule is reported as a natural host of beet curly top, tomato spotted wilt viruses and turnip mosaic, experimentally it was infected with turnip mosaic virus. Eschscholtzia californica Cham. is described as a natural host of aster yellows phytoplasma, and experimental host of bean yellow mosaic virus. In the Czech Republic, only turnip mosaic virus was reliably identified in naturally infected P. somniferum. Keywords: poppy, viruses, Papaver somniferum, P. dubium, P. orientale, P. nudicaule, P. rhoeas, Eschscholtzia californica Journal: Plant Protection Science Pages: 33-36 Volume: 35 Issue: 1 Year: 1999 DOI: 10.17221/9671-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/9671-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-199901-0008.txt Handle: RePEc:caa:jnlpps:v:35:y:1999:i:1:id:9671-PPS