Template-Type: ReDIF-Article 1.0 Author-Name: Lenka Bieliková Author-Workplace-Name: Faculty of Agriculture - Department of Genetics and 2Faculty of Biology - Department of Plant Production, České Budějovice, Czech Republic; 3Mid-Florida Research & Educational Center, IFAS, University of Florida, Apopka, Florida, USA; 4University of South Bohemia, Faculty of Agriculture - Department of Fodder Plants, České Budějovice, Czech Republic Author-Name: Zdeněk Landa Author-Workplace-Name: Faculty of Agriculture - Department of Genetics and 2Faculty of Biology - Department of Plant Production, České Budějovice, Czech Republic; 3Mid-Florida Research & Educational Center, IFAS, University of Florida, Apopka, Florida, USA; 4University of South Bohemia, Faculty of Agriculture - Department of Fodder Plants, České Budějovice, Czech Republic Author-Name: Lance S. Osborne Author-Workplace-Name: Faculty of Agriculture - Department of Genetics and 2Faculty of Biology - Department of Plant Production, České Budějovice, Czech Republic; 3Mid-Florida Research & Educational Center, IFAS, University of Florida, Apopka, Florida, USA; 4University of South Bohemia, Faculty of Agriculture - Department of Fodder Plants, České Budějovice, Czech Republic Author-Name: Vladislav Čurn Author-Workplace-Name: Faculty of Agriculture - Department of Genetics and 2Faculty of Biology - Department of Plant Production, České Budějovice, Czech Republic; 3Mid-Florida Research & Educational Center, IFAS, University of Florida, Apopka, Florida, USA; 4University of South Bohemia, Faculty of Agriculture - Department of Fodder Plants, České Budějovice, Czech Republic Title: Characterization and identification of entomopathogenic and mycoparasitic fungi using RAPD-PCR technique Abstract: Entomopathogenic and mycoparasitic fungi were characterised by RAPD technique, with special attention to evaluate the genetic stability of strains that are used as active ingredients in commercial biopesticides. Strain-specific fingerprints were constructed for Paecilomyces fumosoroseus - strain PFR 97 Apopka, Gliocladium virens - strain GL 21 and Verticillium lecanii - strain MYCOTAL. Genetic stability and homogeneity was confirmed among re-isolates that were obtained from commercial batches of bio-insecticide PFR 97TM 20%WDG and bio-fungicide SoilGardTM12G that had been produced in 1995-1999. RAPD analysis indicated the genetic identity of V. lecanii strains re-isolated from the two different bio-insecticides MYCOTAL® and VERTALEC®. The usefulness of RAPD technique was demonstrated when P. fumosoroseus strain PFR 97 Apopka was reliably identified after having passed through adults of the spruce bark beetle Ips typographus, and by analysis of the relationship between fungi of the genus Gliocladium. Keywords: Gliocladium spp., Gliocladium virens, Paecilomyces fumosoroseus, Verticillium lecanii, entomopathogenic fungi, mycoparasitic fungi, identification using RAPD-PCR Journal: Plant Protection Science Pages: 1-12 Volume: 38 Issue: 1 Year: 2002 DOI: 10.17221/4813-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/4813-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200201-0001.txt Handle: RePEc:caa:jnlpps:v:38:y:2002:i:1:id:4813-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Jiban Kumar Kundu Author-Workplace-Name: Research Institute of Crop Production - Division of Plant Medicine, Prague-Ruzynì, Czech Republic Title: The application of RT-PCR assay for the detection of Apple stem pitting virus and Apple stem grooving virus in four apple cultivars Abstract: The reverse transcription polymerace chain reaction (RT-PCR) assay was successfully used for the detection of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in four apple cultivars of a 25 years old orchard. These two main pome fruit viruses were detected frequently in all tested apple cultivars. ASGV and ASPV occurred in as many as 16 trees (in the cultivar Spartan) and 13 trees (in the cultivar Idared) out of 20 tested trees, respectively. Mixed infection by ASGV and ASPV was found in all tested cultivars (as many as 9 out of 20 tested trees of the cultivar Spartan). Keywords: RT-PCR, ASPV, ASGV, Apple stem pitting virus, Apple stem grooving virus Journal: Plant Protection Science Pages: 13-17 Volume: 38 Issue: 1 Year: 2002 DOI: 10.17221/4816-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/4816-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200201-0002.txt Handle: RePEc:caa:jnlpps:v:38:y:2002:i:1:id:4816-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Bohumír Cagaš Author-Workplace-Name: OSEVA PRO Ltd., Grassland Research Station Rožnov-Zubří, Czech Republic Author-Name: Radek Macháč Author-Workplace-Name: OSEVA PRO Ltd., Grassland Research Station Rožnov-Zubří, Czech Republic Title: Different pathogenicity of ergot isolates (Claviceps purpurea [Fr.] Tul.) on Kentucky bluegrass (Poa pratensis L.) Abstract: Ergot, caused by the fungus Claviceps purpurea (Fr.) Tul., belongs to the main constraints in seed production of Kentucky bluegrass (Poa pratensis L.). The level of ergot occurrence depends on the weather conditions during the growing period, harvest year, storage conditions of seed, and post-harvest treatment. The degree of resistance of the grown cultivar plays an important role. Based on greenhouse trials with four different ergot populations (Zubří, Czech Republic; Leutewitz and Munich, Germany; Pullman, Washington, USA) during 3 years, we found pathogenicity to be one of the most important factors. There were significant differences in the amount of ergot bodies formed between the Central European populations (Zubří, Leutewitz) and the American one from Washington State. The degree of pathogenicity has a major impact on the occurrence of ergot sclerotia in seed of Poa pratensis. Keywords: Poa pratensis L., Claviceps purpurea (Fr.) Tul., isolates, pathogenicity Journal: Plant Protection Science Pages: 18-22 Volume: 38 Issue: 1 Year: 2002 DOI: 10.17221/4815-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/4815-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200201-0003.txt Handle: RePEc:caa:jnlpps:v:38:y:2002:i:1:id:4815-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Jan Macháč Author-Workplace-Name: OSEVA PRO Ltd., Grassland Research Station Rožnov-Zubří, Czech Republic Author-Name: Bohumír Cagaš Author-Workplace-Name: OSEVA PRO Ltd., Grassland Research Station Rožnov-Zubří, Czech Republic Author-Name: Radek Macháč Author-Workplace-Name: OSEVA PRO Ltd., Grassland Research Station Rožnov-Zubří, Czech Republic Title: The effect of some herbicides on seed yield of six grass species - Short Communication Abstract: The tolerance to or phytotoxicity of selected herbicides and their impact on seed yield was investigated in six grasses grown for seed: Phleum pratense cv. Sobol (a), Festuca rubra cv. Táborská (b), Festuca pratensis cv. Otava (c), Poa pratensis cv. Slezanka (d), Arrhenatherum elatius cv. Medián (e) and Dactylis glomerata cv. Zora (f). All species were tolerant to Grodyl 75 WG (amidosulfuron 75%), (a) and (f) were tolerant to Grodyl plus (amidosulfuron 15 g/kg + isoproturon 600 g/kg) which was phytotoxic to the other grass species; all species were tolerant to Stomp 330 E (pendimethalin 330 g/l); Lintur 70 WG (triasulfuron 4.1% + dicamba 65.9%) was phytotoxic only to (c) while the other species were tolerant to it. Keywords: grass, seed, herbicides Journal: Plant Protection Science Pages: 23-28 Volume: 38 Issue: 1 Year: 2002 DOI: 10.17221/4817-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/4817-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200201-0004.txt Handle: RePEc:caa:jnlpps:v:38:y:2002:i:1:id:4817-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Jana Brožová Author-Workplace-Name: Research Institute of Crop Production - Division of Plant Medicine, Prague-Ruzynì, Czech Republic Title: Exploitation of the mycoparasitic fungus Pythium oligandrum in plant protection - A review Abstract: Pythium oligandrum Drechs. belongs to the order Oomycetes. It is parasitic on many fungi from the same or other orders. The antagonism of P. oligandrum is a multifaceted process dependent on the target species involved. P. oligandrum is nonpathogenic on 12 species of crops from six families. It does not attack their tissue but occurs on the root surface, predominantly in the regions of hypocotyl - taproot, together with plant pathogenic fungi. It utilises the root exudates and fungus hyphae on the root surface, including those of the plant pathogens, for its own support. A growth stimulation of plants was observed. P. oligandrum can be utilised for biological control on a wide spectrum of crop plants. Different methods of application have been developed. The effect of a mycoparasite preparation is more preventive. Keywords: biological control, soil fungi, antagonism, rhizosphere, growth stimulation, Botrytis cinerea Journal: Plant Protection Science Pages: 30-35 Volume: 38 Issue: 1 Year: 2002 DOI: 10.17221/4818-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/4818-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200201-0005.txt Handle: RePEc:caa:jnlpps:v:38:y:2002:i:1:id:4818-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Aleš Lebeda Title: R.K. Horst - Westcott's Plant Disease Handbook (6th Edition) - Book Review Journal: Plant Protection Science Pages: 36 Volume: 38 Issue: 1 Year: 2002 DOI: 10.17221/4819-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/4819-PPS.html File-Format: text/html Handle: RePEc:caa:jnlpps:v:38:y:2002:i:1:id:4819-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Jiří Chod Title: Ass. Prof. Ing. Jaroslav Polák, DrSc. - sexagenarian - Biographical Notice Journal: Plant Protection Science Pages: 37-38 Volume: 38 Issue: 1 Year: 2002 DOI: 10.17221/4820-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/4820-PPS.html File-Format: text/html Handle: RePEc:caa:jnlpps:v:38:y:2002:i:1:id:4820-PPS