Template-Type: ReDIF-Article 1.0 Author-Name: Jitka Stará Author-Workplace-Name: Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: František Kocourek Author-Workplace-Name: Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Title: Evaluation of efficacy of Cydia pomonella granulovirus (CpGV) to control the codling moth (Cydia pomonella L., Lep.: Tortricidae) in field trials Abstract: The efficacy of a CpGV-based preparation of Czech production against codling moth (CM) was tested in an experimental apple orchard at Prague-Ruzyně in 1998-2000. The influence of CpGV treatment on the reduction of CM population density and fruit injury was evaluated in comparison with teflubenzuron. Decline of CpGV efficacy in the orchard was tested in laboratory conditions on apples sampled at different terms after CpGV treatment. The rates of CpGV applied ranged from 0.5 to 1.00 × 1013 granulles/ha, and the number of applications from 3 to 5 per year. The biological efficacy of CpGV to reduce the CM population density ranged from 75.5% to 96.0%, that of teflubenzuron from 90.8% to 97.5%, compared to the untreated control. The CpGV treatment was more efficacious in reducing the CM population density than in reducing fruit injury. The efficacy of CpGV decreased to 50% after 20, 10 and 11 d after treatment in 1998, 1999 and 2000, respectively. According to our results, a 10 d interval for CpGV treatments is recommended in case of further mass egg-laying by CM in the period between CpGV applications. Keywords: Cydia pomonella, Cydia pomonella granulovirus, apple orchards Journal: Plant Protection Science Pages: 117-125 Volume: 39 Issue: 4 Year: 2003 DOI: 10.17221/3830-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/3830-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200304-0001.txt Handle: RePEc:caa:jnlpps:v:39:y:2003:i:4:id:3830-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Eva Hrudová Author-Workplace-Name: Department of Plant Protection, Mendel University of Agriculture and Forestry in Brno, Brno, Czech Republic Title: The presence of non-target lepidopteran species in pheromone traps for fruit tortricid moths Abstract: In the localities Brno-Tuřany (Brno-město) and Prakšice (Uherské Hradiště) pheromone traps were used to monitor tortricid moths Adoxophyes orana, Archips podanus, A. rosanus, Hedya nubiferana, Pandemis heparana, Spilonota ocellana, Cydia pomonella, Cydia funebrana and Cydia molesta. Other moths species were also present in these pheromone traps for target species: Pammene albuginana, Cydia coronilana, Notocelia rosaecolana, Hedya pruniana, noctua pronuba, Ypponomeuta malinellus, Pyrausta rectefascialis and P. aurata. Keywords: fruit tortricid moths, pheromone traps, South Moravia, non-target moth species, Noctuidae Journal: Plant Protection Science Pages: 126-131 Volume: 39 Issue: 4 Year: 2003 DOI: 10.17221/3831-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/3831-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200304-0002.txt Handle: RePEc:caa:jnlpps:v:39:y:2003:i:4:id:3831-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Evangelina T. Oparaocha Author-Workplace-Name: Department of Biological Sciences, Michael Okpara University Agriculture, Umuahia, Nigeria Author-Name: Raphael N. Okigbo Author-Workplace-Name: Department of Biological Sciences, Michael Okpara University Agriculture, Umuahia, Nigeria Title: Thrips (Thysanoptera) of vegetable crops (okro, spinach, garden egg and pumpkin) grown in Southeastern Nigeria Abstract: Eight vegetable crops were sampled at five localities in Southeastern Nigeria and were found to harbour one or more species of thrips. The crops were Amaranthus hybridus, A. spinosus, Basella rubra, Solanum incanum, S. melongena, Hibiscus esculentus, Telfairia occidentalis and a species of Solanum. The sampled localities, with the frequency of occurrence of thrips, were Umuahia (29.6%), Owerri (28.5%), Port Harcourt (25.7%), Calabar (18.7%) and Enugu (6.0%). The studies revealed that crops with very heavy infloresences, e.g. Amaranthus hybridus, had a higher number of thrips (40%) per flower/leaf, while simple flowered crops like Telfairia occidentalis could only harbour a far lower number of thrips per flower/leaf. This showed a preference of these thrips for plants with heavy inflorescences which provided them with more protection, especially their larvae. Taxonomic/microscopic studies identified three species of thrips: Haplothrips gowdeyi that attacked 63% of all the sampled crops, Frankliniella schultzei was hosted by 50% of the crops, and Megalurothrips ventralis also preyed on 50% of the plant samples. Keywords: thrips, vegetable crops, Nigeria Journal: Plant Protection Science Pages: 132-138 Volume: 39 Issue: 4 Year: 2003 DOI: 10.17221/3832-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/3832-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200304-0003.txt Handle: RePEc:caa:jnlpps:v:39:y:2003:i:4:id:3832-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Amine Elleuch Author-Workplace-Name: 1 Author-Name: Mohamed Marrakchi Author-Workplace-Name: 1 Author-Name: Dominique Lévesque Author-Workplace-Name: 1 Author-Name: Nabina Bessais Author-Workplace-Name: 1 Author-Name: Jean Pierre Perreault Author-Workplace-Name: 1 Author-Name: Hatem Fakhfakh Author-Workplace-Name: 1 Title: Molecular variability of Citrus exocortis viroid in a single naturally infected citrus tree Abstract: We report the nucleotide sequence of Citrus exocortis viroid (CEVd) from a single natural infected citrus tree of the clementinier variety (Citrus clementina) in Tunisia. The sequence variability of this viroid from its natural host without using an alternative passage by an indicator host or an artificial inoculation was found to occur at 14 positions, giving a variability of 3.8%. This work confirms that naturally - occurring viroids belonging to the Pospiviroid genera contain a mixture of sequence variants. In addition, this study confirms the worldwide distribution of this viroid. Keywords: nucleotide sequence, CEVd, genome, quasi-species Journal: Plant Protection Science Pages: 139-145 Volume: 39 Issue: 4 Year: 2003 DOI: 10.17221/3833-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/3833-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200304-0004.txt Handle: RePEc:caa:jnlpps:v:39:y:2003:i:4:id:3833-PPS Template-Type: ReDIF-Article 1.0 Author-Name: S. Širca Author-Workplace-Name: Agricultural Institute of Slovenia, 1000 Ljubljana, Slovenia. Author-Name: G. Urek Author-Workplace-Name: Agricultural Institute of Slovenia, 1000 Ljubljana, Slovenia. Author-Name: V. Meglič Author-Workplace-Name: Agricultural Institute of Slovenia, 1000 Ljubljana, Slovenia. Title: Molecular and Biochemical Methods Used for the Identification of Globodera Species in Slovenia (from 6th Conference of European Foundation for Plant Pathology, Prague, Czech Republic, 8-14 September 2002) Abstract: Globodera species identification is based on specific morphological characteristics which are included in the majority of keys used for Globodera identification. To confirm and make more precise the morphometrical method, other methods have been developed. At Agricultural Institute of Slovenia, molecular and biochemical methods are applied. With the use of PCR-RFLP method we try to distinguish between G. rostochiensis, G. pallida and G. tabacum. Patterns of nematode DNA digested with restriction endonucleases and subjected to agarose gel electrophoresis are analysed. Differences in DNA sequence result in the number and size of fragments produced (RFLPs). For the differentiation of all three nematodes we have introduced a method for protein electrophoresis. Samples are compared after being separated by polyacrilamide slab gel electrophoresis. Proteins are visualised after silver staining. Keywords: potato cyst nematode, Globodera, identification, PCR, isoelectric focusing (IEF) method Journal: Plant Protection Science Pages: 151-153 Volume: 39 Issue: 4 Year: 2003 DOI: 10.17221/3835-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/3835-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200304-0005.txt Handle: RePEc:caa:jnlpps:v:39:y:2003:i:4:id:3835-PPS Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: Index Volume 39 Journal: Plant Protection Science Pages: X1 Volume: 39 Issue: 4 Year: 2003 DOI: 10.17221/7324-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/7324-PPS.html File-Format: text/html Handle: RePEc:caa:jnlpps:v:39:y:2003:i:4:id:7324-PPS