Template-Type: ReDIF-Article 1.0 Author-Name: Shesh Kumari Author-Workplace-Name: Department of Virology, Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Jiban K. Kundu Author-Workplace-Name: Department of Virology, Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Jaroslav Polák Author-Workplace-Name: Department of Virology, Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Title: Identification of Xiphinema vuittenezi by polymerase chain reaction Abstract: So far, the identification of the nematode species Xiphinema vuittenezi relied mainly on time-consuming morphological and morphometrical studies. Therefore, a polymerase chain reaction (PCR) protocol was optimised that both reliably and rapidly identifies X. vuittenezi. The internal transcribed spacer (ITS) species-specific primer of ribosomal DNA gene of X. vuittenezi was used. Nine populations of this species from Central Bohemia were investigated by means of PCR. Keywords: nematodes, Xiphinema vuittenezi, PCR, ITS primer Journal: Plant Protection Science Pages: 1-4 Volume: 40 Issue: 1 Year: 2004 DOI: 10.17221/3120-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/3120-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200401-0001.txt Handle: RePEc:caa:jnlpps:v:40:y:2004:i:1:id:3120-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Monika Dřímalková Author-Workplace-Name: Department of Plant Protection, Mendel University of Agriculture and Forestry in Brno, Brno, Czech Republic Author-Name: Karel Veverka Author-Workplace-Name: Department of Plant Protection, Mendel University of Agriculture and Forestry in Brno, Brno, Czech Republic Title: Seedlings damping-off of Chenopodium quinoa Willd. Abstract: The causal agents of damping-off of quinoa seedlings were determined in greenhouse experiments. Ascochyta caulina, Fusarium avenaceum, Fusarium spp., Alternaria spp. and Pythium spp. were isolated from infected parts of quinoa seedlings. The most frequent Pythium sp. was P. aphanidermatum. Pathogenicity tests confirmed that P. aphanidermatum and F. avenaceum were the causal agents of damping-off of quinoa seedlings under greenhouse conditions. A comparison of the reaction of quinoa with that of other susceptible plants (spinach, cabbage, sugar beet) showed that quinoa is most susceptible to the pathogen before emergence, during germination till the end of the stage of the first pair of true leaves. Germinable quinoa seeds seemed to have a lower ability to emerge from the soil. This serious problem is caused not only by pre-emergence damping-off from pathogens but more so by a complex of several adverse factors during germination when quinoa is most sensitive. Keywords: seedlings damping-off, Chenopodium quinoa, germination, emergence, Pythium spp., Fusarium spp Journal: Plant Protection Science Pages: 5-10 Volume: 40 Issue: 1 Year: 2004 DOI: 10.17221/3119-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/3119-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200401-0002.txt Handle: RePEc:caa:jnlpps:v:40:y:2004:i:1:id:3119-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Václav Stejskal Author-Workplace-Name: Department of Stored-Product Pest Control, Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Jan Lukáš Author-Workplace-Name: Department of Stored-Product Pest Control, Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Radek Aulický Author-Workplace-Name: Department of Stored-Product Pest Control, Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Title: Temperature-dependent development and mortality of Australian cockroach, Periplaneta australasiae (Fabricius) (Blattodea: Blattidae Abstract: The effect of temperature on the development of the 1st instar of Periplaneta australasiae (Fabr.) was studied at the four constant temperatures of 21°C, 24°C, 27°C and 30°C in temperature-controlled chambers. Mortality was 50% at 30°C, and 10% at 21°C, 24° and 27°C. Thermal constants were established by plotting linear regression to development rate. The thermal threshold for the development was 17.1°C and the thermal constant for 1st instar larvae was 147.1 day-degrees. As "safe temperature" (ts) - the temperature to be maintained in stores or food premises to prevent the development of a pest species - we recommend 16°C. Keywords: Periplaneta australasiae, development, thermal constants, thermal threshold, glasshouses, urban pest Journal: Plant Protection Science Pages: 11-15 Volume: 40 Issue: 1 Year: 2004 DOI: 10.17221/3118-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/3118-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200401-0003.txt Handle: RePEc:caa:jnlpps:v:40:y:2004:i:1:id:3118-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Ogechi N. Agbenin Author-Workplace-Name: Department of Crop Protection, Faculty of Agriculture, Ahmadu Bello University, Zaria, Nigeria Title: Potentials of organic amendments in the control of plant parasitic nematodes Abstract: An increasing number of researchers worldwide are showing interest in organic amendment of soil as means of nematode control. Numerous plant species with nematicidal compounds have been identified. Neem (Azadirachta indica) is considered the best-known example of plants with nematicidal properties and is available commercially in some parts of the world. Its efficiency has been proven locally, with the seed powder giving good control in both field and screenhouse. Several materials are in use as organic amendment. However, the choice of materials for amendment will determine its efficiency in control. The use of organic amendments that are disease-free and with a narrow C:N ratio will improve soil fertility while more efficiently reducing the level of nematodes and minimising the risk of increasing the level of another soil borne pathogens and pest Keywords: plant parasitic nematodes, control, organic amendments Journal: Plant Protection Science Pages: 21-25 Volume: 40 Issue: 1 Year: 2004 DOI: 10.17221/1351-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/1351-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200401-0004.txt Handle: RePEc:caa:jnlpps:v:40:y:2004:i:1:id:1351-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Olalekan Oyeleke Banwo Author-Workplace-Name: Department of Crop Protection, Faculty of Agriculture, Ahmadu Bello University, Zaria, Nigeria Author-Name: Matthew Dada Alegbejo Author-Workplace-Name: Department of Crop Protection, Faculty of Agriculture, Ahmadu Bello University, Zaria, Nigeria Author-Name: Myomodra Emmanuel Abo Author-Workplace-Name: Department of Crop Protection, Faculty of Agriculture, Ahmadu Bello University, Zaria, Nigeria Title: Rice yellow mottle virus genus Sobemovirus: a continental problem in africa Abstract: Rice yellow mottle virus genus sobemovirus (RYMV) was first reported in Kenya in 1966. The disease caused by this virus is fast becoming a major limiting factor in the rice production in Africa and neighbouring islands. It is known to occur in eastern, western and southern African countries and, since very recently, in Central Africa. It is transmitted by insect vectors such as Chaectonema spp., Sesselia pusilla Gerst. and Trichispa sericea Guerin (all Coleoptera) and also by mechanical means. About six strains of the virus now exist. Yield losses caused by the virus range from 20 to 100%. Integrated pest management has been suggested to minimise damage caused by this disease to rice. This paper reviews the characteristics of the virus, symptomatology, host range, distribution, strains, transmission, vector dynamics and virus spread, the economic importance, and management strategies. The need of future research of RYMV is also highlighted. Keywords: Rice yellow mottle virus, Sobemovirus, strains, rice, Africa Journal: Plant Protection Science Pages: 26-36 Volume: 40 Issue: 1 Year: 2004 DOI: 10.17221/1350-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/1350-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200401-0005.txt Handle: RePEc:caa:jnlpps:v:40:y:2004:i:1:id:1350-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Lyuba P.-E. Miteva Author-Workplace-Name: Institute of Plant Physiology Acad. M. Popov, Bulgarian Academy of Sciences, Sofia, Bulgaria Author-Name: Sergei V. Ivanov Author-Workplace-Name: Institute of Plant Physiology Acad. M. Popov, Bulgarian Academy of Sciences, Sofia, Bulgaria Author-Name: Vera S. Alexieva Author-Workplace-Name: Institute of Plant Physiology Acad. M. Popov, Bulgarian Academy of Sciences, Sofia, Bulgaria Author-Name: Emanuil N. Karanov Author-Workplace-Name: Institute of Plant Physiology Acad. M. Popov, Bulgarian Academy of Sciences, Sofia, Bulgaria Title: Effect of atrazine on glutathione levels, glutathione s-transferase and glutathione reductase activities in pea and wheat plants Abstract: Changes were studied in the endogenous level of glutathione (total and oxidised), and in the amount of free thiol groups as caused by the herbicide atrazine on two species of plants with different sensitivity to it. The activities of two enzymes related to glutathione metabolism (glutathione reductase and glutathione S-transferase) were also determined. The application of the herbicide on leaf increased the levels of total and oxidised glutathione in pea and wheat plants. Increased activity glutathione S-transferase in wheat plants was found. Keywords: atrazine, pea, wheat, glutathione, glutathione S-transferase, glutathione reductase Journal: Plant Protection Science Pages: 160-20 Volume: 40 Issue: 1 Year: 2004 DOI: 10.17221/1352-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/1352-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200401-0006.txt Handle: RePEc:caa:jnlpps:v:40:y:2004:i:1:id:1352-PPS