Template-Type: ReDIF-Article 1.0 Author-Name: Jaroslav Polák Author-Workplace-Name: Department of Virology, Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Jitka Pívalová Author-Workplace-Name: Department of Virology, Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Jiří Svoboda Author-Workplace-Name: Department of Virology, Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Title: Prune cv. Jojo resistance to different strains of Plum pox virus Abstract: Trees of prune (Prunus domestica L.), cv. Jojo, were inoculated by chip budding with three different strains of PPV isolated from European plum in the Czech Republic. These isolates included Plum pox virus M strain (PPV-M), Plum pox virus D strain (PPV-D) and a PPV-recombinant both strains (PPV-Rec). The results of the evaluation of the inoculated trees over 2 years are presented. Trees of plum cv. Jojo behaven differently to infection with the three PPV strains. A strong hypersensitive reaction appeared a year after inoculation with PPV-M and PPV-Rec strains, although not all inoculated tree died. PPV must have been present in the tissue of cv. Jojo because the virus was transferred to the rootstock St. Julien. Plants of the rootstock became systemically infected with the PPV-M and PPV-Rec strains, showing severe PPV symptoms. The presence of PPV was proved by ELISA in leaves of rootstock St. Julien, but not in leaves of cv. Jojo. Inoculation with strain PPV-D resulted in partial hypersensitive reaction of plants of cv. Jojo, but after initial stunting and partial death of shoots recovering of plants was observed. Keywords: prune, resistance, hypersensitivity, Plum pox virus, virus strains Journal: Plant Protection Science Pages: 47-51 Volume: 41 Issue: 2 Year: 2005 DOI: 10.17221/2742-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/2742-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200502-0001.txt Handle: RePEc:caa:jnlpps:v:41:y:2005:i:2:id:2742-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Milada Šindelářová Author-Workplace-Name: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic Author-Name: Luděk Šindelář Author-Workplace-Name: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic Title: Isolation of pathogenesis-related proteins from TMV-infected tobacco and their influence on infectivity of TMV Abstract: The composition of pathogenesis-related proteins (PR-proteins) in the intercellular fluid (ICF) and leaf tissue of the hypersensitive tobacco cultivar Xanthi-nc inoculated with Tobacco mosaic virus (TMV), and their inhibitory influence on TMV multiplication were studied. The ICF PR-proteins of infected plants were separated after solubilisation by decreasing gradient of ammonium sulphate, the cell PR-proteins were separated after acidic homogenisation of leaf tissues. The ICF and cell PR-proteins were further purified by ion exchange chromatography on DEAE cellulose. Using discontinuous non-denaturating polyacrylamide gel electrophoresis of DEAE cellulose fractions the PR-proteins were detected. Their molecular weights were estimated by SDS-PAGE. The ICF and cell proteins of infected leaves included PR-proteins of the molecular weights 15-16 kDa (Group 1), 27-28 kDa (Group 3: chitinases) and 36-40 kDa (Group 2a: ڨ-1,3-glucanases). Fractions with different PR-proteins were tested for their effect on infectivity of TMV. Particularly the PR3 and PR2a proteins seem to decrease the infectivity of TMV. Keywords: PR-proteins, PAGE, Tobacco mosaic viru Journal: Plant Protection Science Pages: 52-57 Volume: 41 Issue: 2 Year: 2005 DOI: 10.17221/2747-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/2747-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200502-0002.txt Handle: RePEc:caa:jnlpps:v:41:y:2005:i:2:id:2747-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Josef Hýsek Author-Workplace-Name: Department of Mycology, Division of Phytomedicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Marie Váňová Author-Workplace-Name: Agricultural Research Institute, Ltd., Kroměříž, Czech Republic Author-Name: Jana Hajšlová Author-Workplace-Name: Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic Author-Name: Jana Brožová Author-Workplace-Name: Department of Mycology, Division of Phytomedicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Eliška Sychrová Author-Workplace-Name: Department of Mycology, Division of Phytomedicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Zuzana Radová-Sypecká Author-Workplace-Name: Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic Author-Name: Václav Šíp Author-Workplace-Name: Department of Mycology, Division of Phytomedicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Světlana Sýkorová Author-Workplace-Name: Department of Mycology, Division of Phytomedicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Jana Chrpová Author-Workplace-Name: Department of Mycology, Division of Phytomedicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Ludvík Tvarůžek Author-Workplace-Name: Agricultural Research Institute, Ltd., Kroměříž, Czech Republic Title: Variation in the production of trichothecene mycotoxin deoxynivalenol (DON) in spring barley varieties after treatment with the fungicides azoxystrobin and tebuconazole Abstract: Eight varieties of spring barley (Hordeum vulgaris Lin.) were artificially inoculated with a Fusarium culmorum (W.G. Smith) Saccardo - isolate and naturally infected in the middle of the flowering period, and 2 d later treated with the fungicides azoxystrobin or tebuconazol at a dose of 1 l/ha in 250 l of water. In both control and treated samples of grain the content of deoxynivalenol (DON), the main trichothecene mycotoxin produced by F. culmorum, was determined by gas chromatography (GC-ECD). The treatment with either fungicide resulted in elevated levels of DON, an effect that was more pronounced with azoxystrobin. Keywords: deoxynivalenol, Fusarium culmorum, azoxystrobin, tebuconazole Journal: Plant Protection Science Pages: 58-62 Volume: 41 Issue: 2 Year: 2005 DOI: 10.17221/2745-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/2745-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200502-0003.txt Handle: RePEc:caa:jnlpps:v:41:y:2005:i:2:id:2745-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Shawki Mahmoud Abd-Allah Author-Workplace-Name: Faculty of Agrobiology, Food and Natural Resources, Czech University of Agriculture in Prague, Prague, Czech Republic Author-Name: Vladimír Táborský Author-Workplace-Name: Faculty of Agrobiology, Food and Natural Resources, Czech University of Agriculture in Prague, Prague, Czech Republic Author-Name: František Kamler Author-Workplace-Name: Bee Research Institute, Libčice nad Vltavou, Czech Republic Author-Name: Jan Kazda Author-Workplace-Name: Faculty of Agrobiology, Food and Natural Resources, Czech University of Agriculture in Prague, Prague, Czech Republic Title: Effect of two NeemAzalTM formulations on honeybees under semi-field conditions Abstract: The effects of NeemAzalTM formulations: NeemAzalTM T/S (1% azadirachtin) and NeemAzalTM granules (1% azadirachtin) on honeybees, Apis mellifera L., were studied under semi-field conditions. Three plots at 15 m2 each were sown with spring rape seeds Brassica napus cultivar Likolly (Brassicaceae/Cruciferae). In the first plot NeemAzal granules were added with the seeds during sowing. The second plot was sprayed with NeemAzal T/S during full flowering; GreemaxTM was used as a wetting agent. The third one was sprayed with water only during full flowering as a control. For each treatment one tunnel tent (3 × 5 × 2 m) was used during the flowering period. Small bee colonies were exposed to the treated plants for 7 days. Evaluation was carried out by comparing the results in the treatments to the control and, furthermore, by comparing the pre- and post-application. The mortality in the tunnels and the flight activity were checked before, as well as after the treatment. The development of the bee brood was evaluated by using transparent acetate sheets to mark single cells in brood combs with their contents on different assessment dates. The time schedule of the assessment dates was chosen in order to check the bee brood at different expected stages during the development. The development of the bee brood was evaluated by calculation of brood termination rates in percentage and brood indices. The results show that residues of NeemAzal granules did not adversely affect bee mortality, foraging activity or brood development. By contrast, it was noticed that NeemAzal T/S caused some reduction in foraging activity and brood development. Keywords: Apis mellifera, NeemAzalTM, GreemaxTM, mortality, foraging activity, brood development Journal: Plant Protection Science Pages: 63-72 Volume: 41 Issue: 2 Year: 2005 DOI: 10.17221/2744-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/2744-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200502-0004.txt Handle: RePEc:caa:jnlpps:v:41:y:2005:i:2:id:2744-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Josef Šedivý Author-Workplace-Name: Department of Entomology, Division of Phytomedicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Pavel Cihlař Author-Workplace-Name: Faculty of Agrobiology, Food and Natural Resources, Czech University of Agriculte in Prague, Prague, Czech Republic Title: Infestation of poppy cultures with the poppy stem gall wasp (Timaspis papaveris) Cynipidae: Hymenoptera Abstract: The mortality of pupae in stems lying on the ground during hibernation was 51.7% in 2003. The emergence of adults in 2003 and 2004 was monitored from late April to early July by photoeclectors placed on fields that grew poppy the previous year. Most adult gall wasps emerged in April to early May 2004. The occurrence of females ovipositing on poppy stems varied from April to July and the degree of damage to the plants increased. Most frequently the females oviposited in the first stem internodes. The infestation of poppy cultures with the gall wasps was almost uniform, the number of infested plants increasing still in June. In late June, only seven out of 600 plants were not infested. Green sticks covered with non-coagulating glue and placed in the poppy field, indicated the stem heights frequented by ovipositing females. The gall wasp larvae were parasitised by Trichomalus bracteatus Walker and Pseudotorymus papaveris Ruschka, with T. bracteatus predominating. Most frequently, gall wasp larvae inside the seventh internode were parasitised by this species. Keywords: Timaspis papaveris Kiefer, poppy culture, damage, mortality, crop infestation, parasitisation Journal: Plant Protection Science Pages: 73-79 Volume: 41 Issue: 2 Year: 2005 DOI: 10.17221/2746-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/2746-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200502-0005.txt Handle: RePEc:caa:jnlpps:v:41:y:2005:i:2:id:2746-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Marek Renčo Author-Workplace-Name: Parasitological Institute, Slovak Academy of Sciences, Košice, Slovak Republic Title: Current occurrence and distribution of heterodera avenae in the Slovak Republic Abstract: During 2003 and 2004 the occurrence and distribution of Heterodera avenae (Woll.) in the cereal growing areas of the Slovak Republic was studied. 188 soil samples from 27 districts throughout the country were analysed; Heterodera avenae was present in 56.4% of the samples, at an incidence of 2-81 cysts in 100 g soil. At 87 localities the incidence of the parasite was low, at 15 it was medium, and high at 4 localities. Keywords: Heterodera avenae (Woll.), occurrence, Slovak Republic, cereal cyst nematodes Journal: Plant Protection Science Pages: 80-85 Volume: 41 Issue: 2 Year: 2005 DOI: 10.17221/2740-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/2740-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200502-0006.txt Handle: RePEc:caa:jnlpps:v:41:y:2005:i:2:id:2740-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Zuzana Kučerová Author-Workplace-Name: Department of Stored-Product Pest Control, Division of Phytomedicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Radek Aulický Author-Workplace-Name: Department of Stored-Product Pest Control, Division of Phytomedicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Author-Name: Václav Stejskal Author-Workplace-Name: Department of Stored-Product Pest Control, Division of Phytomedicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic Title: Outdoor occurrence of stored-product pests (Coleoptera) in the vicinity of a grain store - Short communication Abstract: The species composition, frequency and abundance of insect pests (Coleoptera) occurring outside a grain store were explored. Wheat bait traps were used for pest monitoring; they were changed every month from April to September. For the first time the outdoor occurrence of primary and secondary stored-product pests was documented in the Czech Republic. Seven species of Coleoptera were found outside the grain store. Pest abundance and frequency were in high correlation. The primary pest Sitophilus granarius was dominant. Keywords: Coleoptera, S. granarius, grain bait traps, outdoor infestation Journal: Plant Protection Science Pages: 86-89 Volume: 41 Issue: 2 Year: 2005 DOI: 10.17221/2739-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/2739-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200502-0007.txt Handle: RePEc:caa:jnlpps:v:41:y:2005:i:2:id:2739-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Kamila Bacigálová Author-Workplace-Name: Institute of Botany, Slovak Academy of Sciences, Bratislava, Slovak Republic Author-Name: Dezider Tóth Author-Workplace-Name: Department of Genetics and Plant Breeding, Faculty of Agrobiology and Food Resources, Slovak Agricultural University in Nitra, Nitra, Slovak Republic Author-Name: Ján Brindza Author-Workplace-Name: Department of Genetics and Plant Breeding, Faculty of Agrobiology and Food Resources, Slovak Agricultural University in Nitra, Nitra, Slovak Republic Title: Powdery mildew Phyllactinia corni causing disease on Cornus mas (Cornaceae) - a new record for Slovakia Abstract: Phyllactinia corni, a powdery mildew on Cornus mas, is reported for the first time from Slovakia. The conidial state and mature cleistothecia found at two locations in Slovakia are described and illustrated. Keywords: Cornus mas, Phyllactinia corni, Phyllactinia guttata, powdery mildew Journal: Plant Protection Science Pages: 90-93 Volume: 41 Issue: 2 Year: 2005 DOI: 10.17221/2741-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/2741-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200502-0008.txt Handle: RePEc:caa:jnlpps:v:41:y:2005:i:2:id:2741-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Karel Veverka Title: Prof. Ing. ZDENĚK ČAČA, DrSc. (1927-2005) - Obituary notice Journal: Plant Protection Science Pages: 94 Volume: 41 Issue: 2 Year: 2005 DOI: 10.17221/2743-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/2743-PPS.html File-Format: text/html Handle: RePEc:caa:jnlpps:v:41:y:2005:i:2:id:2743-PPS