Template-Type: ReDIF-Article 1.0 Author-Name: Jaroslav Polák Author-Workplace-Name: Department of Virology, Division of Plant Health, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Milan Jokeš Author-Workplace-Name: Department of Virology, Division of Plant Health, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Miloslava Ducháčová Author-Workplace-Name: Department of Virology, Division of Plant Health, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Alena Hauptmanová Author-Workplace-Name: Department of Virology, Division of Plant Health, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Petr Komínek Author-Workplace-Name: Department of Virology, Division of Plant Health, Crop Research Institute, Prague-Ruzyně, Czech Republic Title: Electron microscopy of structures present in embryonic cells of plants infected with Plum pox virus Abstract: Electron microscopy was used to detect the presence of virus particles or inclusions in growth tips and parenchymatic cells of leaves of plum, apricot and peach trees artificially infected with Plum pox virus (PPV). Typical pinwheels were found in ultrathin sections of leaves of PPV infected plums, apricots and peaches. Filamentous particles or their aggregates approximately 750 nm long were found in ultrathin sections of growth tips of plum, apricot, and peach shoots with a diameter of 0.5 mm. Pinwheels were never present in embryonic cells. No virus particles were found in ultrathin sections of growth tips of PPV infected plum, apricot and peach shoots of 0.2 mm in diameter. Embryonic cells of growth tips up to 0.2 mm in diameter are PPV free. PPV particles are present in growth tips at a distance 0.2-0.5 mm from the top; the virus is probably multiplied in this part of the growth tips. Keywords: sharka, herbaceous and woody hosts, meristematic cells, growth tips, PPV aggregates, ultrathin sections Journal: Plant Protection Science Pages: 81-84 Volume: 44 Issue: 3 Year: 2008 DOI: 10.17221/28/2008-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/28/2008-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200803-0001.txt Handle: RePEc:caa:jnlpps:v:44:y:2008:i:3:id:28-2008-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Václav Krejzar Author-Workplace-Name: Department of Bacteriology, Division of Plant Medicine, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Josef Mertelík Author-Workplace-Name: The Silva Tarouca Research Institute for Landscape and Ornamental Gardening, Průhonice, Czech Republic Author-Name: Iveta Pánková Author-Workplace-Name: Department of Bacteriology, Division of Plant Medicine, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Kateřina Kloudová Author-Workplace-Name: The Silva Tarouca Research Institute for Landscape and Ornamental Gardening, Průhonice, Czech Republic Author-Name: Václav Kůdela Author-Workplace-Name: Department of Bacteriology, Division of Plant Medicine, Crop Research Institute, Prague-Ruzyně, Czech Republic Title: Pseudomonas marginalis associated with soft rot of Zantedeschia spp. Abstract: For the first time in the Czech Republic, bacteria identified as Pseudomonas marginalis, Pectobacterium carotovorum subsp. carotovorum and Pseudomonas putida were isolated from tubers of Zantedeschia spp. with symptoms of tuber soft rot. The symptoms occurred on mother tubers as well as on new daughter tubers of different calla lily hybrids with yellow spathe, calla lily cv. Mango with bright orange spathe and Zantedeschia rehmanii with pink spathe. The percentage of diseased plants of the total plants in the plot was around 10%. When inoculated into potato tuber slices, strains of P. marginalis and P. c. subsp. carotovorum produced soft rot. Pectolytic activity of P. marginalis strains was less intensive than that of the P. c. subsp. carotovorum strain. The results confirm that bacterial soft rot of Zantedeschia spp. may have several causes. Keywords: bacterial soft rot, Zantedeschia spp., Pseudomonas marginalis, Pseudomonas putida, Pectobacterium carotovorum subsp. carotovorum, Czech Republic Journal: Plant Protection Science Pages: 85-90 Volume: 44 Issue: 3 Year: 2008 DOI: 10.17221/16/2008-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/16/2008-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200803-0002.txt Handle: RePEc:caa:jnlpps:v:44:y:2008:i:3:id:16-2008-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Josef KORBA Author-Workplace-Name: Department of Bacteriology, Division of Plant Medicine, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Jana ŠILLEROVÁ Author-Workplace-Name: Department of Bacteriology, Division of Plant Medicine, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Václav KŮDELA Author-Workplace-Name: Department of Bacteriology, Division of Plant Medicine, Crop Research Institute, Prague-Ruzyně, Czech Republic Title: Resistance of apple varieties and selections to Erwinia amylovora in the Czech Republic Abstract: Sixty-four apple cultivars and selections of potential interest to apple producers and plant breeders in the Czech Republic were tested for their relative resistance to the fire blight pathogen over six years. Level of fire blight resistance was evaluated according to the extent of lesion development on the shoots tips after artificial inoculation in experimental plots under insectproof nets. Cultivars Quinte (resistant) and Yellow transparent (high susceptible) were included in the tests. Of 64 apple cultivars and selections tested, none were high resistant, 3.1% were evaluated as resistant, 10.9% moderately resistant, 57.8% moderately susceptible, 21.9% susceptible and 6.3% high susceptible. Resistant apple genotypes, showing blight necrosis of shoots of 11-12%, were only cultivars Selena and Quinte. Moderately resistant genotypes (blight necrosis 13.1-25.0%) were Kordona, Golden Smoothee, Julia, HL 323, Melodie, HL 421 and S 634/3. High susceptible genotypes (blight necrosis more then 80.1%) were comprised cultivars Vesna, Topas, Yellow transparent and Vanda. The remaining genotypes were moderately susceptible (blight necrosis 26.1-60.0%) and susceptible (blight necrosis 60.1-80.0%). During six experimental years, quantitative variability was recorded in the blight score. Differences between cultivars in susceptibility to fire blight were often statistically significant. Keywords: apple cultivars, Erwinia amylovora, fire blight resistance Journal: Plant Protection Science Pages: 91-96 Volume: 44 Issue: 3 Year: 2008 DOI: 10.17221/19/2008-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/19/2008-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200803-0003.txt Handle: RePEc:caa:jnlpps:v:44:y:2008:i:3:id:19-2008-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Ivana Šafránková Author-Workplace-Name: Department of Crop Science, Breeding and Plant Medicine, Faculty of Agronomz, Mendel University of Agriculture and Forestry Brno, Brno Czech Republic Title: New records of Chrysomyxa rhododendri on Rhododendron species Abstract: In the spring of 2008, leaf spot was observed on Rhododendron hippophaeoides and Rhododendron obtusum; the causal agent was identified as the rust Chrysomyxa rhododendri. The rust occurs primarily in mountain regions and in the Czech Republic was first observed 105 years ago. In the present study we describe pathogen morphology and symptoms on the two new host species. Keywords: Chrysomyxa rhododendri, Rhododendron hippophaeoides, Rh. Obtusum Journal: Plant Protection Science Pages: 97-100 Volume: 44 Issue: 3 Year: 2008 DOI: 10.17221/27/2008-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/27/2008-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200803-0004.txt Handle: RePEc:caa:jnlpps:v:44:y:2008:i:3:id:27-2008-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Mohd Zainudin Nur Ain Izzati Author-Workplace-Name: Department of Biology, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia Author-Name: Faridah ABDULLAH Author-Workplace-Name: Department of Biology, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia Title: Disease suppression in Ganoderma-infected oil palm seedlings treated with Trichoderma harzianum Abstract: Disease suppression in Ganoderma-infected oil palm seedlings treated with a conidial suspension of Trichoder-ma harzianum FA 1132 was tested in plant house conditions to determine the effectiveness of the fungus as a biocontrol agent. The highest efficacy of control was achieved by treatment right after artificial infection; the total number of infected plants was reduced to give the lowest disease severity index (DSI) value of 5.0%, compared to the infected and non-treated control that had the highest DSI of 70.0%. After conidia suspension of FA 1132 was applied, the colony forming ability by Trichoderma in the soil was dramatically increased, but decreased after some time. Results of the present study are a useful reference basis for further tests in the field and large scale production trials. Keywords: Ganoderma boninense, Trichoderma harzianum, oil palm, biological control, basal stem rot Journal: Plant Protection Science Pages: 101-107 Volume: 44 Issue: 3 Year: 2008 DOI: 10.17221/23/2008-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/23/2008-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200803-0005.txt Handle: RePEc:caa:jnlpps:v:44:y:2008:i:3:id:23-2008-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Monika JELOKOVÁ Author-Workplace-Name: Department of Plant Protection, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Nitra, Slovak Republic Author-Name: Ján Gallo Author-Workplace-Name: Department of Plant Protection, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Nitra, Slovak Republic Title: Parasitoids of cereal leaf beetle, Oulema gallaeciana (Heyden, 1879) Abstract: Cereal leaf beetles are serious animal pests of crops in many areas of western and eastern Slovakia. Research was carried out in these areas in order to receive knowledge on occurrence of natural enemies of Oulema gallaeciana. We were detecting particular species of parasitoids parasitising Oulema gallaeciana and their spreading in particular area. The results showed that dominant parasitoid was Necremnus leucarthros. Other parasitoids with bigger proportion on parasitisation were Pteromalus vibulenus and Diplazon spp. with parasitisation up to 30%. In respect of significant parasitisation of Oulema gallaeciana by parasitoid Necremnus leucarthros, it would be appropriate to use this species for biological control of cereal leaf beetle. Keywords: cereal leaf beetle, Oulema gallaeciana, parasitoids, Necremnus leucarthros, Pteromalus vibulenus, crops, temperature, biological control Journal: Plant Protection Science Pages: 108-113 Volume: 44 Issue: 3 Year: 2008 DOI: 10.17221/1260-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/1260-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200803-0006.txt Handle: RePEc:caa:jnlpps:v:44:y:2008:i:3:id:1260-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Lukáš Varga Author-Workplace-Name: Department of Zoology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic Title: Hercinothrips femoralis (Reuter, 1891) - a new pest thrips (Thysanoptera: Panchaetothripinae) in Slovakia Abstract: Monitoring of pest species in plant production and extended trade on ornamental plant material, including precise identification, are the main goals of modern plant protection. As a result, a new species of phytophagous thrips - Hercinothrips femoralis (Reuter, 1891) was recorded in Slovakia (experimental greenhouse in Comenius University, Bratislava) in mid 2007. Some plant specimens infested by this pest showed signs of lesions especially on young leaves. The species has been reported in more European countries recently. Due to the potential damage it can cause, increased attention should be paid to pest monitoring. Keywords: Hercinothrips femoralis, greenhouse, ornamental plants, Slovakia Journal: Plant Protection Science Pages: 114-118 Volume: 44 Issue: 3 Year: 2008 DOI: 10.17221/13/2008-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/13/2008-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-200803-0007.txt Handle: RePEc:caa:jnlpps:v:44:y:2008:i:3:id:13-2008-PPS Template-Type: ReDIF-Article 1.0 Author-Name: František Kocourek Title: Obituary of Associate Professor RNDr. JOSEF ŠEDIVÝ, DrSc. (1925-2008) Journal: Plant Protection Science Pages: 119-120 Volume: 44 Issue: 3 Year: 2008 DOI: 10.17221/1266-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/1266-PPS.html File-Format: text/html Handle: RePEc:caa:jnlpps:v:44:y:2008:i:3:id:1266-PPS