Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: Index of Volume 46 (2010), Authors Index, Authors Institution Index, List of Reviewers, Subject Index Journal: Plant Protection Science Pages: I-VII Volume: 46 Issue: 4 Year: 2010 DOI: 10.17221/1276-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/1276-PPS.html File-Format: text/html Handle: RePEc:caa:jnlpps:v:46:y:2010:i:4:id:1276-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Jaroslav Polák Author-Workplace-Name: Department of Virology, Division of Plant Health, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Ivan Oukropec Author-Workplace-Name: Mendel University in Brno, Faculty of Horticulture, Lednice na Moravě, Czech Republic Title: Identification of interspecific peach and Prunus sp. hybrids resistant to Plum pox virus infection Abstract: Interspecific hybrids of Prunus persica, Barier, Fire, Cadaman, GF-677, and Prunus sp. hybrids and selections, MRS, NBS 540-73, and Pumiselect were evaluated for resistance to Plum pox virus. Hybrids were grafted onto trees of a peach cultivar artificially infected with PPV and evaluated for six years for resistance to the virus. The relative concentration of PPV protein was determined by semiquantitative ELISA in June every year. The presence of PPV in peach hybrids was confirmed by IC-RT-PCR in 2007-2008. The presence and intensity of PPV symptoms were evaluated monthly from May to September. The hybrid GF-677 (P. amygdalus × P. persica) was confirmed as highly resistant to PPV. Hybrids Cadaman (P. davidiana × P. persica) and Fire (P. amygdalus × P. persica) were characterized as resistant to PPV. Hybrids GF-677, Cadaman and Fire were selected as candidate sources of resistance to be crossed with peach cultivars susceptible to PPV. Keywords: Sharka disease, peach, Prunus sp., sources of resistance, determination, intensity of symptoms, semiquantitative ELISA, IC-RT-PCR detection Journal: Plant Protection Science Pages: 139-144 Volume: 46 Issue: 4 Year: 2010 DOI: 10.17221/11/2010-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/11/2010-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201004-0002.txt Handle: RePEc:caa:jnlpps:v:46:y:2010:i:4:id:11-2010-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Aleš EICHMEIER Author-Workplace-Name: Faculty of Horticulture in Lednice, Mendeleum - Institute of Genetics, Mendel University in Brno, Lednice, Czech Republic Author-Name: Miroslav BARÁNEK Author-Workplace-Name: Faculty of Horticulture in Lednice, Mendeleum - Institute of Genetics, Mendel University in Brno, Lednice, Czech Republic Author-Name: Miroslav PIDRA Author-Workplace-Name: Faculty of Horticulture in Lednice, Mendeleum - Institute of Genetics, Mendel University in Brno, Lednice, Czech Republic Title: Analysis of genetic diversity and phylogeny of partial coat protein domain in Czech and Italian GFLV isolates Abstract: The genetic diversity of Grapevine fanleaf virus (GFLV) was evaluated in 4 isolates sampled from naturally infected grapevines from South Moravia (Czech Republic) and 2 Italian isolates from Bari (Italy). Conserved regions within sequences in databases were found and new primers corresponding to these regions were designed and tested for RT-PCR amplification of the CP codifying region. After sequencing of obtained amplicons the similarity of isolates was analysed via alignments of sequences and by means of dendrograms Keywords: Grapevine fanleaf virus, grapevine, coat protein, sequence Journal: Plant Protection Science Pages: 145-148 Volume: 46 Issue: 4 Year: 2010 DOI: 10.17221/10/2010-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/10/2010-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201004-0003.txt Handle: RePEc:caa:jnlpps:v:46:y:2010:i:4:id:10-2010-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Entsar Ibrahim Rabea Author-Workplace-Name: Department of Pest Control and Environmental Protection, Faculty of Agriculture, Alexandria University, Damanhour, Egypt Author-Name: Walter Steurbaut Author-Workplace-Name: Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Gent, Belgium Title: Chemically modified chitosans as antimicrobial agents against some plant pathogenic bacteria and fungi Abstract: The antimicrobial activity of eight chemically modified chitosans against plant pathogenic bacteria of crown gall disease Agrobacterium tumefaciens and soft mould disease Erwinia carotovora and fungi of early blight disease Alternaria alternata, root rot disease Fusarium oxysporum, and damping off disease Pythium debaryanum was investigated. The minimum inhibitory concentration (MIC) of these compounds against the tested bacteria showed that N-(o,o-dichlorobenzyl)chitosan exhibited a high activity against A. tumefaciens and E. carotovora (MIC = 500 and 480 mg/l, respectively). In addition, the antifungal behaviour was investigated in vitro on mycelial growth and spore germination. N-(p-fluorobenzyl)chitosan was the most active against the mycelial growth of A. alternata with an EC50 of 703.4 mg/l, while the N-(o-chloro,o-fluorobenzyl)chitosan exhibited a high activity against F. oxysporum and P. debaryanum (EC50 = 641.2 and 155.7 mg/l, respectively). Against the fungal spore germination, all modified chitosans showed a higher inhibition of spore germination than unmodified chitosan. The compound of N-(o,o-di fluorobenzyl)chitosan exhibited a high inhibition percentage against A. alternata. However, N-(o-chlorobenzyl)chitosan and N-(p-chlorobenzyl)chitosan were significantly highly active against the spore germination of F. oxysporum. Keywords: chemically modified chitosans, antibacterial activity, antifungal activity, spore germination Journal: Plant Protection Science Pages: 149-158 Volume: 46 Issue: 4 Year: 2010 DOI: 10.17221/9/2009-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/9/2009-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201004-0004.txt Handle: RePEc:caa:jnlpps:v:46:y:2010:i:4:id:9-2009-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Hamdy Mahmoud EL-SHARABASY Author-Workplace-Name: Plant Protection Department and Author-Name: Ahmed IBRAHIM Author-Workplace-Name: Soil and Water Department, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt Title: Communities of oribatid mites and heavy metal accumulation in oribatid species in agricultural soils in Egypt impacted by waste water Abstract: The continued use of waste water for irrigation of agricultural fields in Egypt may lead to accumulation of heavy metals in soils and adverse effects on soil-living communities. We investigated responses of oribatid communities to heavy metal contamination in mango plantations irrigated by the Ismailia canal in the Suez region. Mean concentrations of heavy metals determined in irrigation water were considerably above the recommended levels. Concentrations of metals in agricultural soil were however below the permissible levels. A comparison with concentrations of a typical uncontaminated soil in this area revealed that the Ismailia water canal used for irrigation of agricultural land has elevated levels of heavy metals. The results of our ecological survey showed that the abundance and structure of the soil oribatid communities were not influenced by levels of heavy metals in the soil. We also showed that the diversity index can be a valuable tool for assessing the possible impact of pollutants on different species of oribatid mites. The oribatid species appeared to be accumulating different amounts of heavy metals when characterised by their bioconcentration factors. Most species were poor zinc accumulators. The accumulation of heavy metals in the body of oribatids was not strictly determined by their body size or by the trophic level. In conclusion, our study showed that mango plantations impacted by waste water from the Ismailia canal are accumulating heavy metals in their soils above the background concentrations, but ecological effects on soil-living communities are not apparent yet. Keywords: oribatid mites, diversity, bioindicators, soil pollution, water pollution, heavy metals Journal: Plant Protection Science Pages: 159-170 Volume: 46 Issue: 4 Year: 2010 DOI: 10.17221/31/2010-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/31/2010-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201004-0005.txt Handle: RePEc:caa:jnlpps:v:46:y:2010:i:4:id:31-2010-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Ondřej Douda Author-Workplace-Name: Department of Entomology, Division of Plant Heath, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Miloslav Zouhar Author-Workplace-Name: Department of Plant Protection, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague-Suchdol, Czech Republic Author-Name: Eva Nováková Author-Workplace-Name: Department of Plant Protection, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague-Suchdol, Czech Republic Author-Name: Jana Mazáková Author-Workplace-Name: Department of Plant Protection, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague-Suchdol, Czech Republic Author-Name: Pavel Ryšánek Author-Workplace-Name: Department of Plant Protection, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague-Suchdol, Czech Republic Title: Variability of D2/D3 segment sequences of several populations and pathotypes of potato cyst nematodes (Globodera rostochiensis, Globodera pallida) Abstract: Potato cyst nematodes (Globodera rostochiensis, Globodera pallida) remain a key pest in the main potato growing regions of the Czech Republic. Due to difficult direct management and presence of diverse pathotypes attacking different potato cultivars the rapid and reliable diagnostics is of crucial importance. Currently, efforts are aimed at a description of different pathotypes based on DNA analysis. The main objective of this study was to evaluate the homogeneity of sequences of D2/D3 segments of the 28S rDNA gene obtained from 3 populations of G. rostochiensis and 5 populations of G. pallida and estimate their value for diagnostic purposes. PCR amplification yielded a single fragment of the length of 700 bp approximately in all populations. The alignment score of the vast majority of all pair comparisons of G. rostochiensis and G. pallida populations varied from 98 to 99. In total 14 point deletions and 3 substitutions were observed. The variability of D2/D3 segments of potato cyst nematodes is rather low and this DNA region can be used for diagnostics on a species level because more differences were found after comparing with G. tabacum and G. millefolii sequences obtained from Gene Bank; however the applicability of D2/D3 sequences to routine diagnostics of potato cyst nematodes could be complicated by its similarity to corresponding sequences of the nematode G. artemisiae. Keywords: Globodera rostochiensis, G. pallida, pathotype, D2/D3 segment Journal: Plant Protection Science Pages: 171-180 Volume: 46 Issue: 4 Year: 2010 DOI: 10.17221/1/2010-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/1/2010-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201004-0006.txt Handle: RePEc:caa:jnlpps:v:46:y:2010:i:4:id:1-2010-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Nadežda Ondejková Author-Workplace-Name: Section of Diagnostics, Central Control and Testing Institute of Agriculture in Bratislava, Bratislava, Slovak Republic Author-Name: Michaela Hudecová Author-Workplace-Name: Section of Diagnostics, Central Control and Testing Institute of Agriculture in Bratislava, Bratislava, Slovak Republic Author-Name: Kamila Bacigálová Author-Workplace-Name: Institute of Botany, Slovak Academy of Sciences, Bratislava, Slovak Republic Title: First report on Monilinia fructicola in the Slovak Republic Abstract: The occurrence of Monilinia on stone and pome fruit trees in Slovakia was studied. Commonly distributed species Monilinia laxa, M. fructigena, and American species M. fructicola were determined by the methods used. M. fructicola was identified on the fruits of peach and nectarine imported to our country and on domestic plum fruits as well. To our knowledge, this is the first report on M. fructicola in Slovakia and a new member for Slovak mycobiota. Keywords: Monilinia laxa, Monilinia fructigena, Monilinia fructicola, new record Journal: Plant Protection Science Pages: 181-184 Volume: 46 Issue: 4 Year: 2010 DOI: 10.17221/1271-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/1271-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201004-0007.txt Handle: RePEc:caa:jnlpps:v:46:y:2010:i:4:id:1271-PPS Template-Type: ReDIF-Article 1.0 Author-Name: J. Marková Title: Book Review Bacigálová K.: Mycota (Huby). Ascomycota (Vreckaté huby). Taphrinomycetes: Taphrinales (Grmanníkotvaré), čeľ. Protomycetaceae, čeľ. Taphrinaceae - Flóra Slovenska X/2 Journal: Plant Protection Science Pages: 188 Volume: 46 Issue: 4 Year: 2010 DOI: 10.17221/50/2010-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/50/2010-PPS.html File-Format: text/html Handle: RePEc:caa:jnlpps:v:46:y:2010:i:4:id:50-2010-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Jakub BERÁNEK Author-Workplace-Name: Unit of Integrated Plant Protection Methods, State Phytosanitary Administration, Brno, Czech Republic Author-Name: Ivana ŠAFRÁNKOVÁ Author-Workplace-Name: Department of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Czech Republic Title: First Record of Horidiplosis ficifolii Harris 2003 (Diptera: Cecidomyiidae) in the Czech Republic Abstract: In November 2009 the gall midge species Horidiplosis ficifolii Harris 2003 was detected in the Czech Republic on leaves of an ornamental fig Ficus microcarpa. The insect is native to South East Asia, where it lives on the genus Ficus. No information is currently available on the prevalence of the pest in the countries of its origin. Imported Ficus plants from China into the Netherlands are sometimes heavily infested with the gall midge. In Europe it is also known from Denmark and the United Kingdom. As Horidiplosis ficifolii is a tropical species, no environmental and social damage is expected. Keywords: Diptera, Cecidomyiidae, Holodiplosis ficifolii, Ficus, pest Journal: Plant Protection Science Pages: 189-191 Volume: 46 Issue: 4 Year: 2010 DOI: 10.17221/53/2009-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/53/2009-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201004-0009.txt Handle: RePEc:caa:jnlpps:v:46:y:2010:i:4:id:53-2009-PPS