Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: Index of Volume 48 Author Index, Author Institution Index, Subject Index Journal: Plant Protection Science Pages: I-VII Volume: 48 Issue: 4 Year: 2012 DOI: 10.17221/6396-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/6396-PPS.html File-Format: text/html Handle: RePEc:caa:jnlpps:v:48:y:2012:i:4:id:6396-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Jaroslav POLÁK Author-Workplace-Name: Department of Virology, Division of Plant Health, Crop Research Institute, Prague-Ruzyně, Czech Republic Author-Name: Petr KOMÍNEK Author-Workplace-Name: Department of Virology, Division of Plant Health, Crop Research Institute, Prague-Ruzyně, Czech Republic Title: Biological evidence for practical immunity of apricot cultivar Harlayne to Plum pox virus Abstract: Ten-year results of the practical immunity investigation of apricot cv. Harlayne are presented. Two-year-old trees of cv. Harlayne were inoculated by chip-budding with six different strains and isolates of Plum pox virus (PPV). PPV inoculated trees grew in the field and were evaluated from 2001 to 2011. No PPV symptoms appeared in the leaves of cv. Harlayne within ten years (2002-2011), and within eight years (2004-2011) in the fruits and stones. None of the six isolates of three different PPV strains was detected in the leaves and fruits by ELISA. Suckers of the rootstock Prunus myrobalana developed around cv. Harlayne trees in 2005-2011 were symptomless and ELISA was negative within seven years. New trees of cv. Harlayne obtained from tested trees by budding on PPV susceptible apricot rootstock MVA-2 in 2007 were PPV free from 2008 through 2011. The presence of PPV was proved by ELISA neither in leaves of cv. Harlayne nor in rootstock MVA-2. Keywords: stone fruits, immunity, PPV strains, symptoms, bud transmission, susceptible rootstock, runners Journal: Plant Protection Science Pages: 143-148 Volume: 48 Issue: 4 Year: 2012 DOI: 10.17221/31/2011-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/31/2011-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201204-0002.txt Handle: RePEc:caa:jnlpps:v:48:y:2012:i:4:id:31-2011-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Rakib A. Al-Ani Author-Workplace-Name: Plant Protection Department, College of Agriculture and Author-Name: Mustafa A. Adhab Author-Workplace-Name: Plant Protection Department, College of Agriculture and Author-Name: Majda H. Mahdi Author-Workplace-Name: Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq Author-Name: Hadi M. Abood Author-Workplace-Name: Ministry of Sciences and Technology, Baghdad, Iraq$2 Title: Rhizobium japonicum as a biocontrol agent of soybean root rot disease caused by Fusarium solani and Macrophomina phaseolina Abstract: The activity of Rhizobium japonicum against the soil-borne pathogens Fusarium solani and Macrophomina phaseolina as causative agents of soybean root rot disease in both culture medium and soil was evaluated. Rhizobial culture filtrate caused an inhibition of the fungal radial growth of Fusarium solani and Macrophomina phaseolina on potato dextrose agar medium amended with the filtrate compared with control. The addition of rhizobial culture suspension to the soil contaminated by the two pathogens, Fusarium solani and Macrophomina phaseolina and their interaction, in pots, improved seed germination percentages and reduced the root rot disease index significantly. The sowing of rhizobial coated seeds in soil contaminated by Fusarium solani and Macrophomina phaseolina separately and in combination, in the field, increased seed germination significantly and induced a high reduction in disease severity for the same previous combination under field conditions. These results indicate that rhizobia could be an important element in root rot disease management. Keywords: biological control, Rhizobium sp., soybean, Fusarium, Macrophomina Journal: Plant Protection Science Pages: 149-155 Volume: 48 Issue: 4 Year: 2012 DOI: 10.17221/16/2012-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/16/2012-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201204-0003.txt Handle: RePEc:caa:jnlpps:v:48:y:2012:i:4:id:16-2012-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Pavel Kopecký Author-Workplace-Name: Centre of the Haná Region for Biotechnological and Agricultural Research, Department of Genetic Resources for Vegetables, Medicinal and Special Plants, Crop Research Institute, Olomouc, Czech Republic Author-Name: Ivana Doležalová Author-Workplace-Name: Centre of the Haná Region for Biotechnological and Agricultural Research, Department of Genetic Resources for Vegetables, Medicinal and Special Plants, Crop Research Institute, Olomouc, Czech Republic Author-Name: Martin Duchoslav Author-Workplace-Name: Department of Botany, Faculty of Science, Palacký University, Olomouc, Czech Republic Author-Name: Karel Dušek Author-Workplace-Name: Centre of the Haná Region for Biotechnological and Agricultural Research, Department of Genetic Resources for Vegetables, Medicinal and Special Plants, Crop Research Institute, Olomouc, Czech Republic Title: Variability in resistance to clubroot in European cauliflower cultivars Abstract: Fifty genotypes of cauliflovwer (Brassica oleracea var. botrytis) were evaluated for resistance to clubroot disease (Plasmodiophora brassicae Wor.) under controlled conditions in a plant growth chamber. The cultivars with the highest resistance were Brilant, Agora, and Bora, while the most susceptible were the cultivars White Top, White Fox, and Octavian. The variation in disease index is probably due to different pathogenicity rates of clubroot pathotypes and genetic heterogeneity of European cauliflower cultivars. The obtained results will be tested in an infested and non-infested field. Keywords: Brassica oleracea var. botrytis, germplasm, Plasmodiophora brassicae Wor., disease resistance Journal: Plant Protection Science Pages: 156-161 Volume: 48 Issue: 4 Year: 2012 DOI: 10.17221/58/2011-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/58/2011-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201204-0004.txt Handle: RePEc:caa:jnlpps:v:48:y:2012:i:4:id:58-2011-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Somayeh Dariush Author-Workplace-Name: Department of Plant Pathology, Faculty of Agricultural Sciences, Guilan University, Guilan, Iran Author-Name: Ali Akbar Ebadi Author-Workplace-Name: Rice Research Institute of Iran, Rasht, Iran Author-Name: Maryam Khoshkdaman Author-Workplace-Name: Rice Research Institute of Iran, Rasht, Iran Author-Name: Babak Rabiei Author-Workplace-Name: Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, Guilan University, Guilan, Iran Author-Name: Ali Elahinia Author-Workplace-Name: Department of Plant Pathology, Faculty of Agricultural Sciences, Guilan University, Guilan, Iran Title: Characterising the genetic diversity of Pseudomonas syringae pv. syringae isolated from rice and wheat in Iran Abstract: Sheath rot of rice and leaf blight of wheat caused by Pseudomonas syringae pv. syringae are the important bacterial pathogens of rice and wheat in Iran. The randomly amplified polymorphic DNA (RAPD) method was used to investigate the genetic diversity of 60 strains of P. s. pv. syringae obtained from rice and wheat in different growth stages. Cluster analysis by UPGMA method showed that strains were grouped into two clusters. The AMOVA analysis indicated that about 18% of the total genetic variation existed between two populations of rice and wheat, which showed the lack of host specialization in P. s. pv. syringae strains among rice and wheat. We confirmed that high genetic heterogeneity existed in the P. s. pv. syringae strains which are detectable by RAPD analysis, and that molecular and statistical analysis of RAPD fragments can be used both to distinguish between strains and to determine relatedness between them. Keywords: bacterial pathogen, population genetic structure, host preference, RAPD Journal: Plant Protection Science Pages: 162-169 Volume: 48 Issue: 4 Year: 2012 DOI: 10.17221/64/2011-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/64/2011-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201204-0005.txt Handle: RePEc:caa:jnlpps:v:48:y:2012:i:4:id:64-2011-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Mohamed S. Khalil Author-Workplace-Name: Central Agricultural Pesticides Laboratory, Agriculture Research Center, El-Sabaheya, Alexandria, Egypt Author-Name: Mohamed E. I. Badawy Author-Workplace-Name: Department of Pesticide Chemistry and Technology, Faculty of Agriculture, Alexandria University, El-Shatby, Alexandria, Egypt Title: Nematicidal activity of a biopolymer chitosan at different molecular weights against root-knot nematode, Meloidogyne incognita Abstract: The nematicidal activity of four molecular weights (2.27 × 105, 3.60 × 105, 5.97 × 105, and 9.47 × 105 g/mol) of a biopolymer chitosan was assayed against the root-knot nematode, Meloidogyne incognita, in vitro and in pot experiments. In laboratory assays, the nematode mortality was significantly influenced by exposure times and chitosan molecular weight. Low molecular weight chitosan (2.27 × 105 g/mol) was the most effective in killing the nematode with EC50 of 283.47 and 124.90 mg/l after 24 and 48 h of treatment, respectively. In a greenhouse bioassay, all the compounds mixed in soil at one- and five-fold concentrations of the LC50 value significantly reduced population, egg mass, and root galling of tomato seedlings compared with the untreated control. In general, the nematicidal activity of these compounds was increased dramatically with a decrease in the molecular weight. The results suggest that the chitosan at low molecular weight may serve as a natural nematicide Keywords: in vitro and plot experiment, molecular weight, natural nematicide, mematode mortality Journal: Plant Protection Science Pages: 170-178 Volume: 48 Issue: 4 Year: 2012 DOI: 10.17221/46/2011-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/46/2011-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201204-0006.txt Handle: RePEc:caa:jnlpps:v:48:y:2012:i:4:id:46-2011-PPS