Template-Type: ReDIF-Article 1.0 Author-Name: Roman Hałaj Author-Workplace-Name: The Upper Silesian Nature Society, Katowice, Poland Author-Name: Barbara Osiadacz Title: Woolly ash aphid - is the alien bug posing a threat to European ash trees? - a review Abstract: Prociphilus (Meliarhizophagus) fraxinifolii (Riley, 1879) (Hemiptera: Eriosomatidae) is an alien species in Europe that has been spreading quickly there. This paper presents the origin and current occurrence, classification, description, identification, and biology of this species. It also discusses the harmfulness and threats, especially the impact on host- plants and ecosystem, invasion rate and facilitating factors. Keywords: Aphidoidea, biocapacity, climate changes, invasiveness forecast, species acclimatization, zoogeography Journal: Plant Protection Science Pages: 127-133 Volume: 53 Issue: 3 Year: 2017 DOI: 10.17221/138/2016-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/138/2016-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201703-0001.txt Handle: RePEc:caa:jnlpps:v:53:y:2017:i:3:id:138-2016-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Parisa Mohammadi Author-Workplace-Name: Department of Plant Protection and Author-Name: Elif Tozlu Author-Workplace-Name: Department of Plant Protection and Author-Name: Recep Kotan Author-Workplace-Name: Department of Plant Protection and Author-Name: Merve Şenol Kotan Author-Workplace-Name: Department of Agricultural Biotechnology, Faculty of Agriculture, Ataturk University, Erzurum, Turkey Title: Potential of some bacteria for biological control of postharvest citrus green mould caused by Penicillium digitatum Abstract: Ten bacteria isolate (4 Bacillus subtilis, 2 Bacillus pumilus, 2 Bacillus cereus, 1 Bacillus megaterium, and 1 Agrobacterium radiobacter) were tested in vitro for antagonistic properties against Penicillium digitatum, the causal agent of citrus green mould. The effect of these bacteria was also observed on mycelial growth, spore germination, and spore production of the pathogenic fungus in broth culture. Extracellular enzyme activities of the bacteria were determined. According to the results of in vitro antagonistic tests and enzymes activities, the most promising bacteria were Bacillus subtilis and Agrobacterium radiobacter. These bacteria were tested for disease suppression on lemon fruits. In addition, these bacterial isolates also showed remarkable antifungal activity against the pathogen on lemon fruits. The results of this study showed that Bacillus subtilis and Agrobacterium radiobacter showed remarkable antifungal activity against the pathogen. Chitinase and glucanase enzyme activity of all the tested bacteria was positive. Protease enzyme activity was positive in all tested bacteria with the exception of Agrobacterium radiobacter. In addition, all bacteria inhibited mycelial growth and spore germination (except Agrobacterium radiobacter) of the fungus. Bacillus subtilis, Bacillus cereus, and Agrobacterium radiobacter inhibited spore production in broth culture. Bacillus subtilis and Agrobacterium radiobacter were tested on lemon fruits significantly reduced disease severity. Consequently, these isolates can be used as new biocontrol agents in controlling the post-harvest decay of citrus fruits caused by Penicillium digitatum. Keywords: Bacillus spp., biocontrol, citrus green mould, postharvest disease, Agrobacterium radiobacter Journal: Plant Protection Science Pages: 134-143 Volume: 53 Issue: 3 Year: 2017 DOI: 10.17221/55/2016-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/55/2016-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201703-0002.txt Handle: RePEc:caa:jnlpps:v:53:y:2017:i:3:id:55-2016-PPS Template-Type: ReDIF-Article 1.0 Author-Name: María de Jesús Ramírez-Ramírez Author-Workplace-Name: Postgraduate Studies and Research Division, Technological Institute of Tlajomulco, National Institute of Technology of Mexico, Tlajomulco de Zúñiga, Jal, Mexico Author-Name: N. Alejandra Mancilla-Margalli Author-Workplace-Name: Postgraduate Studies and Research Division, Technological Institute of Tlajomulco, National Institute of Technology of Mexico, Tlajomulco de Zúñiga, Jal, Mexico Author-Name: Lucía Meza-Álvarez Author-Workplace-Name: Postgraduate Studies and Research Division, Technological Institute of Tlajomulco, National Institute of Technology of Mexico, Tlajomulco de Zúñiga, Jal, Mexico Author-Name: Ramón Turincio-Tadeo Author-Workplace-Name: Postgraduate Studies and Research Division, Technological Institute of Tlajomulco, National Institute of Technology of Mexico, Tlajomulco de Zúñiga, Jal, Mexico Author-Name: Doralinda Guzmán-De Pena Author-Workplace-Name: Department of Biotechnology and Biochemistry, Cinvestav Irapuato, Irapuato, Gto., Mexico Author-Name: Martin Eduardo Avila-Miranda Title: Epidemiology of Fusarium agave wilt in Agave tequilana Weber var. azul Abstract: Fusarium oxysporum is reported as the principal causal agent limiting production of Agave tequilana Weber var. azul, but frequent isolation of F. solani, and symptoms typical of F. solani as a pathogen like severe reddish coloured root rot and loss of soil anchorage are frequently associated with diseased agaves. Inoculations of agave plantlets with F. solani induced typical agave root rot symptoms in greenhouse trials. The incidence of both pathogens was determined molecularly with specific primers in the ITS2 sequence. Dispersion patterns of agave wilt, determined in plantations of different age, indicated a tendency to produce aggregated patterns over time as the disease spread from the initial symptomatic plant to adjacent plants. Although both fungi were isolated from agave diseased plants, and in spite of the higher percentage of detection and root rot symptoms, it is concluded that F. solani may have a greater impact in agave wilt. Keywords: Pathozone, soil-borne, plant pathogens, dispersion pattern Journal: Plant Protection Science Pages: 144-152 Volume: 53 Issue: 3 Year: 2017 DOI: 10.17221/142/2016-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/142/2016-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201703-0003.txt Handle: RePEc:caa:jnlpps:v:53:y:2017:i:3:id:142-2016-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Irena Petrželová Author-Name: Michaela Jemelková Author-Workplace-Name: Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Genetic Resources for Vegetables, Medicinal and Special Plants, Crop Research Institute , Olomouc, Czech Republic Author-Name: Ivana Doležalová Author-Workplace-Name: Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Genetic Resources for Vegetables, Medicinal and Special Plants, Crop Research Institute , Olomouc, Czech Republic Author-Name: Vladan Ondřej Author-Workplace-Name: Department of Botany, Faculty of Science, Palacky University in Olomouc, Olomouc, Czech Republic Author-Name: Miloslav Kitner Author-Workplace-Name: Department of Botany, Faculty of Science, Palacky University in Olomouc, Olomouc, Czech Republic Title: Identification of a rust disease of giant knapweed in the Czech Republic - short communication Abstract: During the vegetation seasons 2012-2015, symptoms of severe infections by a rust disease were recorded on plants of the ornamental species Centaurea macrocephala. Based on morphology, the pathogen was identified as Puccinia jaceae or Puccinia hieracii, which have recently been considered as synonyms. However, substantial differences between P. jaceae and P. hieracii in nucleotide sequences of the ITS2 region provide evidence for the molecular identification of the specimen as P. jaceae. Keywords: Centaurea macrocephala, Grossheimia macrocephala, ITS rDNA, Puccinia hieracii, Puccinia jaceae Journal: Plant Protection Science Pages: 153-158 Volume: 53 Issue: 3 Year: 2017 DOI: 10.17221/117/2016-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/117/2016-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201703-0004.txt Handle: RePEc:caa:jnlpps:v:53:y:2017:i:3:id:117-2016-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Vojtěch Hlavjenka Author-Workplace-Name: Department of Plant Protection, Agritec Plant Research, Ltd., Šumperk, Czech Republic Author-Name: Marek Seidenglanz Author-Workplace-Name: Department of Plant Protection, Agritec Plant Research, Ltd., Šumperk, Czech Republic Author-Name: Aleš Dufek Author-Workplace-Name: Agriresearch Rapotín, Ltd., Vikýřovice, Czech Republic Author-Name: Hana Šefrová Author-Workplace-Name: Department of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic$2 Title: Spatial distribution of cabbage root maggot (Delia radicum) and clubroot (Plasmodiophora brassicae) in winter oilseed rape crops in the Czech Republic Abstract: The amount and spatial distribution of plants afflicted with cabbage root maggot (Delia radicum; CRM) and clubroot (Plasmodiophora brassicae) in winter oilseed rape crops were assessed in the Olomouc region (Northern Moravia, Czech Republic) over the course of 2012-2014. A total of 16 commercial rape fields were included in the assessments. Plants with tumours showed a significantly lower (P < 0.001) level of infestation induced by CRM (24% of plants infested) compared to plants without tumours (37% of plants infested). According to a generalised linear mixed model, plants with thicker hypocotyls are predisposed to significantly higher levels (P < 0.001) of root surface damage induced by CRM. The correlation analysis indicates rather weak or intermediate levels of correlation between the two variables (hypocotyls thickness × root surface damage induced by CRM). Both CRM and clubroot symptomatic plants showed a significant tendency for aggregation in rape crops, but not in all cases. Distributions of CRM and clubroot symptomatic plants were either significantly spatially dissociated or not associated in crops. Ovipositing D. radicum females showed some tendency to avoid zones with higher number of plants infected by P. brassicae. Distributions of CRM and hypocotyl thickness levels were significantly spatially associated in crops in several cases. Keywords: within-field distribution of pests in crops, spatial associations between pests in crops, Integrated Plant Management, control of insect pests, winter oilseed rape diseases Journal: Plant Protection Science Pages: 159-168 Volume: 53 Issue: 3 Year: 2017 DOI: 10.17221/181/2015-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/181/2015-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201703-0005.txt Handle: RePEc:caa:jnlpps:v:53:y:2017:i:3:id:181-2015-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Radek Aulicky Author-Workplace-Name: Crop Research Institute, Prague, Czech Republic Author-Name: Vlastimil Kolar Author-Workplace-Name: Podravka-Lagris, Dolní Lhota u Luhačovic, Czech Republic Author-Name: Jan Plachy Author-Workplace-Name: DDD servis s.r.o. Praha, Prague, Czech Republic Author-Name: Vaclav Stejskal Author-Workplace-Name: Crop Research Institute, Prague, Czech Republic Title: Field efficacy of brief exposure of adults of six storage pests to nitrogen-controlled atmospheres Abstract: The efficacy of a brief exposure (1, 7, and 10 days) to a nitrogen-controlled atmosphere (N-CA) for major storagepests in a field validation study in the Czech Republic is reported. The main goal was to estimate how quickly themobile adult stages of six species of storage beetles (Oryzaephilus surinamensis, Cryptolestes ferrugineus, Triboliumconfusum, Tribolium castaneum, Sitophilus granarius, and Sitophilus oryzae) are killed after introduction of theinfested commodity to prevent their further spread to the surrounding storage bins. The trials were conducted in ametal bin containing 25 t of seeds using the system of continual top-down nitrogen filling to replace the oxygen. Thecomposition of N-CA in the silo was measured continually. The target N-CA concentration (i.e., ≤ 1% O2 and 99% N2)was reached at the bottom of the silo after 12 h of the purging phase of nitrogen silo filling. A one-day exposure toN-CA corresponds to top-down filling, which initially gives higher concentrations of N2 in the upper than in the lowerpart of the silo: low efficacy was reached at the silo bottom (0-33.3%), while higher efficacy (16.7-100%) was reachedat the top of the silo bin. The mortality variation at both locations was species dependent: the most sensitive was O.surinamensis, and the least sensitive were S. granarius and S. oryzae. Seven days of N-CA exposure led to 100% mortalityof all tested species except for S. granarius (96.7% mortality at the bottom), while 10 days of N-CA exposure ledto 100% mortality of all adults located at both the bottom and the top of the silo. This experiment showed that oneday of exposure to N-CA caused significant mortality to reduce the spread of insects from the top of the silo but notfrom the silo bottom, and 10 days of exposure completely prevent the adult mobile pest stages of all tested speciesfrom spreading from the treated silo and causing cross-infestation in the storage facility. Keywords: non-chemical pest control, physical methods, modified atmosphere, metal silo bin, stored grain Journal: Plant Protection Science Pages: 169-176 Volume: 53 Issue: 3 Year: 2017 DOI: 10.17221/136/2016-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/136/2016-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201703-0006.txt Handle: RePEc:caa:jnlpps:v:53:y:2017:i:3:id:136-2016-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Rudolf Masarovič Author-Workplace-Name: Department of Environmental Ecology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic Author-Name: Martin Štefánik Author-Workplace-Name: Department of Environmental Ecology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic Author-Name: Martina Zvaríková Author-Workplace-Name: Department of Environmental Ecology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic Author-Name: Jakub Sigmund Author-Workplace-Name: Department of Environmental Ecology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic Author-Name: Peter Fedor Title: First record of a new alien economically important thrips Dichromothrips corbetti (Priesner, 1936) (Thysanoptera: Thripidae) in Slovakia - short communication Abstract: The first record of vanda thrips (Dichromothrips corbetti [Priesner, 1936], Thysanoptera, Thripidae) in Slovakia is presented. An economically important pest with heavy infestations on flowers of Phalaenopsis orchids was recorded as introduced most probably from North African plantations. Potential infiltration of this exotic species into Central European glasshouses refers to the globalisation of trade in biological commodities. Keywords: orchids, pest, Phalaenopsis spp., vanda thrips Journal: Plant Protection Science Pages: 177-180 Volume: 53 Issue: 3 Year: 2017 DOI: 10.17221/141/2016-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/141/2016-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201703-0007.txt Handle: RePEc:caa:jnlpps:v:53:y:2017:i:3:id:141-2016-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Jan Kabíček Title: Phytoseiid mites on Quercus cerris in an urban park - short communication Abstract: The community of phytoseiid mites on the leaves of non-indigenous Quercus cerris was studied in an intensively managed urban park during the years 2012-2014. Five phytoseiid species, namely Kampimodromus aberrans, Typhloseiulus peculiaris, Euseius finlandicus, Typhlodromus (Typhlodromus) pyri, and Paraseiulus triporus, were found on the studied oak leaves; three of them are generalist predators. K. aberrans and T. peculiaris were the dominant species (88.5% of all sampled phytoseiids), of which K. aberrans was the significantly most abundant species on the inspected oak leaves. Non-native Q. cerris can serve as a favourable host plant and refuge for certain phytoseiid species in environmentally unfriendly urban areas. Keywords: Phytoseiidae, Acari, turkey oak, species diversity, host plant Journal: Plant Protection Science Pages: 181-186 Volume: 53 Issue: 3 Year: 2017 DOI: 10.17221/167/2016-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/167/2016-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201703-0008.txt Handle: RePEc:caa:jnlpps:v:53:y:2017:i:3:id:167-2016-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Christoph Kunz Author-Name: Dominic J. Sturm Author-Workplace-Name: Department of Weed Science, Institute of Phytomedicine, University of Hohenheim, Stuttgart, Germany Author-Name: Markus Sökefeld Author-Workplace-Name: Department of Weed Science, Institute of Phytomedicine, University of Hohenheim, Stuttgart, Germany Author-Name: Roland Gerhards Author-Workplace-Name: Department of Weed Science, Institute of Phytomedicine, University of Hohenheim, Stuttgart, Germany Title: Weed suppression and early sugar beet development under different cover crop mulches Abstract: Field experiments were conducted at two locations in 2014-2015 and 2015-2016 to investigate the weed suppressive ability of cover crop mulches in sugar beets. Three cover crops and two cover crop mixtures were tested in all four experiments. Weed densities ranged from 2 up to 210 plants/m2 in Chenopodium album L. and Stellaria media (L.) Vill. as predominant species. Sinapis alba grew significantly faster than Vicia sativa, Raphanus sativus var. niger, and both cover crop mixtures. Sinapis alba, Vicia sativa, Raphanus sativus var. niger reduced weed density by 57, 22, and 15% across all locations, respectively. A mixture of seven different cover crops reduced weed emergence by 64% compared to the control plot without cover crop mulch. Early sugar beet growth was enhanced by all mulch treatments in 2015 and decelerated in 2016. Keywords: Beta vulgaris L., Chenopodium album L., conservation tillage, cover crop mixture, integrated weed management, intercropping, Stellaria media (L.) Journal: Plant Protection Science Pages: 187-193 Volume: 53 Issue: 3 Year: 2017 DOI: 10.17221/109/2016-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/109/2016-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201703-0009.txt Handle: RePEc:caa:jnlpps:v:53:y:2017:i:3:id:109-2016-PPS