Template-Type: ReDIF-Article 1.0 Author-Name: Qaiser Jamal Author-Workplace-Name: Division of Food Technology, Biotechnology and Agrochemistry, Institute of Environmentally-Friendly Agriculture, Chonnam National University, Gwangju, South Korea Author-Name: Yong Seong Lee Author-Workplace-Name: Division of Food Technology, Biotechnology and Agrochemistry, Institute of Environmentally-Friendly Agriculture, Chonnam National University, Gwangju, South Korea Author-Name: Hyeon Deok Jeon Author-Workplace-Name: Division of Food Technology, Biotechnology and Agrochemistry, Institute of Environmentally-Friendly Agriculture, Chonnam National University, Gwangju, South Korea Author-Name: Kil Young Kim Title: Effect of plant growth-promoting bacteria Bacillus amylliquefaciens Y1 on soil properties, pepper seedling growth, rhizosphere bacterial flora and soil enzymes Abstract: The Bacillus amyloliquefaciens Y1 strain was evaluated for its effects on soil properties, pepper seedling growth, rhizosphere bacterial flora and soil enzyme activities. Y1 solubilised insoluble phosphate, produced chitinase, and released siderophores in plate detection assay. In order to evaluate the plant growth promotion potential in vivo, strain Y1 was grown in media containing chitin powder and complex fertiliser. The pot experiment was conducted by treating pepper seedlings with C1/1 (Y1 culture, 50 ml), C2/3 (Y1 culture, 33 ml), C1/2 (Y1 culture, 25 ml), F1/1 (complex fertiliser, 50 ml), F1/2 (complex fertiliser, 25 ml), and W (water) at 10, 20, 30, 40, and 50 days after transplantation (DAT). Plants receiving Y1 had 52% (C1/2) and 68% (C1/1) more root and shoot biomass than W, and 14% (C1/1) and 18% (C2/3) more compared to F1/1 at 80 DAT. Total numbers of flowers per plant at 80 DAT were found significantly higher with the application of Y1 having 34 (C1/1), 35 (C2/3), and 22 (C1/2) compared to 4 (W), 12 (F1/1) and 10 (F1/2). In addition, chlorophyll content in pepper leaves was found to improve with the application of Y1. Furthermore, Y1 has significantly improved nutritional assimilation of total NPK, population of total culturable bacteria and chitinase producing bacteria and activities of chitinase and dehydrogenase in soil. At 60 and 80 DAT, the number of B. amyloliquefaciens at C1/1, C2/3, and C1/2 ranged from 2.3 × 104 to 4.6 × 104 CFU/g of soil. Our results concluded that B. amyloliquefaciens Y1 has positive effects on soil properties and can be suggested as a bio-fertiliser to minimise fertiliser application in modern agriculture. Keywords: siderophore, chitin powder, chitinase, PGPR Journal: Plant Protection Science Pages: 129-137 Volume: 54 Issue: 3 Year: 2018 DOI: 10.17221/154/2016-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/154/2016-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201803-0001.txt Handle: RePEc:caa:jnlpps:v:54:y:2018:i:3:id:154-2016-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Sumer Horuz Author-Workplace-Name: Plant Protection Department, Faculty of Agriculture, Cukurova University, Adana, Turkey Author-Workplace-Name: Plant Protection Department, Faculty of Agriculture, Erciyes University, Kayseri, Turkey Author-Name: Yesim Aysan Author-Workplace-Name: Plant Protection Department, Faculty of Agriculture, Cukurova University, Adana, Turkey Title: Biological control of watermelon seedling blight caused by Acidovorax citrulli using antagonistic bacteria from the genera Curtobacterium, Microbacterium and Pseudomonas Abstract: The biological control of the watermelon seedling blight and fruit blotch disease was investigated by screening the potential use of antagonistic bacteria. Between May and August 2012, totally 322 putative antagonistic bacteria were isolated from symptomless melon and watermelon plants grown in Adana, Hatay, and Osmaniye provinces of the Eastern Mediterranean Region of Turkey. In vitro dual culture tests showed that 54 out of 322 strains inhibited the Acidovorax citrulli (Ac) growth with an appearance of clear zones between 2.3 and 27.0 mm in diameter. However, the remaining 268 strains did not exhibit any antagonistic activity against Ac. Seed treatments with fourteen individual antagonistic bacteria resulted in a significant reduction in disease incidence (DI) and severity (DS) ranging between 14.06-79.47% and between 4.57-41.49%, respectively. The bacteria Pseudomonas oryzihabitans (Antg-12), Microbacterium oxydans (Antg-57), Curtobacterium flaccumfaciens (Antg-198), and Pseudomonas fluorescens (Antg-273) were the most potent antagonistic bacterial isolates which reduced DI and DS as compared to the untreated control. This study suggested the potential of bacterial antagonists Curtobacterium flaccumfaciens, Microbacterium oxydans, Pseudomonas oryzihabitans, and Pseudomonas fluorescens for the biocontrol of Ac-induced bacterial fruit blotch (BFB). Keywords: Acidovorax citrulli, cucurbist, biocontrol, seed treatment Journal: Plant Protection Science Pages: 138-146 Volume: 54 Issue: 3 Year: 2018 DOI: 10.17221/168/2016-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/168/2016-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201803-0002.txt Handle: RePEc:caa:jnlpps:v:54:y:2018:i:3:id:168-2016-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Ilknur Polat Author-Name: Ömür Baysal Author-Workplace-Name: Department of Molecular Biology and Genetics, Faculty of Science, Mugla Sitki Koçman University, Mugla, Turkey Author-Name: Emine Gümrükcü Author-Workplace-Name: Bati Akdeniz Agricultural Research Institute (BATEM), Antalya, Turkey Author-Name: Görkem Sülü Author-Workplace-Name: Bati Akdeniz Agricultural Research Institute (BATEM), Antalya, Turkey Author-Name: Aytül Kitapci Author-Workplace-Name: Bati Akdeniz Agricultural Research Institute (BATEM), Antalya, Turkey Author-Name: Ramazan Özalp Author-Workplace-Name: Bati Akdeniz Agricultural Research Institute (BATEM), Antalya, Turkey Author-Name: Ibrahim Çelik Author-Workplace-Name: Bati Akdeniz Agricultural Research Institute (BATEM), Antalya, Turkey Author-Name: Zübeyir Devran Author-Workplace-Name: Mediterranean University, Faculty of Agriculture, Antalya, Turkey Author-Name: Ersin Polat Author-Workplace-Name: Mediterranean University, Faculty of Agriculture, Antalya, Turkey Title: Molecular diversity and assessment of reactions of pepper pure line germplasm to Botrytis cinerea Abstract: The host resistance level of pure line materials was assessed in the genepool for the purpose of breeding. The highest resistance to the pathogen was observed in bell-type pepper. Moreover, genetic diversity of pure lines was investigated using selected inter-simple sequence repeat (ISSR) primers. Generally, genetic markers showed genetic diversity, so that long-type pure lines were separated from the other accessions. This is the first report on host reactions of Turkish pure lines as breeding material. These results provide significant information for future pepper breeding programs. Keywords: disease severity, genetic diversity, grey mould, ISSR, reaction Journal: Plant Protection Science Pages: 147-152 Volume: 54 Issue: 3 Year: 2018 DOI: 10.17221/44/2017-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/44/2017-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201803-0003.txt Handle: RePEc:caa:jnlpps:v:54:y:2018:i:3:id:44-2017-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Ali Sarkhosh Author-Workplace-Name: Katherine Research Station, Department of Primary Industry and Resources, Northern Territory Government, South Stuart Highway, Katherine, Australia Author-Workplace-Name: Tropical Research and Education Center, University of Florida, Homestead, USA Author-Name: Bruce Schaffer Author-Workplace-Name: Tropical Research and Education Center, University of Florida, Homestead, USA Author-Name: Ana I. Vargas Author-Workplace-Name: Tropical Research and Education Center, University of Florida, Homestead, USA Author-Name: Aaron J. Palmateer Author-Workplace-Name: Tropical Research and Education Center, University of Florida, Homestead, USA Author-Name: Patricia Lopez Author-Workplace-Name: Tropical Research and Education Center, University of Florida, Homestead, USA Author-Name: Asiye Soleymani Author-Workplace-Name: Department of Chemistry and Biochemistry, Florida International University, Miami, USA Title: In vitro evaluation of eight plant essential oils for controlling Colletotrichum, Botryosphaeria, Fusarium and Phytophthora fruit rots of avocado, mango and papaya Abstract: In vitro efficacy of the essential oils extracted from eight plant species was tested at application rates of 100, 250, 500, 1000, or 2000 µl/l for controlling fruit rots. Results showed a 100% reduction of mycelium growth of Colletotrichum, Fusarium, Phytophthora, Botryosphaeria after applying thyme or savory oils at all concentrations tested. Mint and cinnamon oils inhibited mycelium growth of the five isolates at application rates of either 1000 or 2000 µl/l. Tea tree, lavender, myrtle, and eucalyptus oils were slightly effective at controlling mycelium growth of each fungus species tested. Savory oil with major constituent of carvacrol 71.2% and thyme oil with major constituent of thymol 73.3% showed the greatest potential of the essential oils tested for use as natural fungicides. Keywords: fruit rots, mycelium growth, natural fungicides, Satureja khuzistanica Journal: Plant Protection Science Pages: 153-162 Volume: 54 Issue: 3 Year: 2018 DOI: 10.17221/49/2017-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/49/2017-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201803-0004.txt Handle: RePEc:caa:jnlpps:v:54:y:2018:i:3:id:49-2017-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Martin Žabka Author-Name: Roman Pavela Author-Workplace-Name: Secondary Plant Metabolites in Crop Protection, Division of Crop Protection and Plant Health, Crop Research Institute, Prague, Czech Republic Title: Effectiveness of environmentally safe food additives and food supplements in an in vitro growth inhibition of significant Fusarium, Aspergillus and Penicillium species Abstract: We tested 38 legislatively recognised substances such as food additives and supplements for antifungal efficacy, with the aim of providing an alternative to synthetic conventional fungicides. These compounds were tested against 9 significant pathogenic fungal species belonging to the significant genera Fusarium, Penicillium, and Aspergillus. Of these compounds, 6 are proposed as potential candidates to provide a complementary alternative to conventional fungicides. Natamycin provided extreme efficacy expressed as MIC50 (5-31 µg/ml), followed by BHA and then BHT, CaNa2EDTA, PABA, and chitosan expressed as MIC50 (0.7-1.9 mg/ml). Safety and antifungal activity were discussed in terms of the mode of action and molecular structure, as well as in terms of potential practical use and legislative requirements for the introduction into practice. We presume that food additives and food supplements are definitely a great source of antifungal compounds. In developed areas of the world (e.g. in the EU), they could represent legislatively recognised compounds, so-called basic substances. Keywords: pathogenic fungi, toxigenic fungi, plant protection, basic substances, health risk, safe antifungals Journal: Plant Protection Science Pages: 163-173 Volume: 54 Issue: 3 Year: 2018 DOI: 10.17221/86/2017-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/86/2017-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201803-0005.txt Handle: RePEc:caa:jnlpps:v:54:y:2018:i:3:id:86-2017-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Piotr Szulc Author-Workplace-Name: Department of Agronomy and Author-Name: Jan Bocianowski Author-Workplace-Name: Department of Mathematical and Statistical Methods, Poznań University of Life Sciences, Poznań, Poland Author-Name: Kamila Nowosad Author-Workplace-Name: Department of Genetics, Plant Breeding and Seed Production, Wrocław University of Environmental and Life Sciences, Wrocław, Poland Author-Name: Tadeusz Michalski Author-Workplace-Name: Department of Agronomy and Author-Name: Hubert Waligóra Author-Workplace-Name: Department of Agronomy and Author-Name: Paweł Olejarski Author-Workplace-Name: Department of Knowledge Transfer and Innovation, Institute of Plant Protection, National Research Institute, Poznań, Poland$2 Title: Assessment of the influence of fertilisation and environmental conditions on maize health Abstract: Field experiments showed the occurrence of such agrophages as the frit fly (Oscinella frit L.) and the European maize borer (Pyrausta nubilalis Hbn.). Identified diseases included fusariosis (Fusarium ssp.) and maize smut (Ustilago maydis Corda). The incidence of the frit fly was influenced by weather conditions, mainly temperature, in the period from sowing to the BBCH 15-16 phase. Moderate temperature conditions contributed to the occurrence of the pest. The significantly highest percentage of plants damaged by larvae of this pest was recorded on maize fertilised only with potassium (K) and phosphorus with potassium (PK). In turn, the lowest percentage of plants damaged by frit fly larvae was recorded for maize fertilised with nitrogen and potassium (NK). The percentage of plants damaged by the European maize borer was influenced by temperature and humidity conditions in individual years of the study. The highest percentage of plants damaged by larvae of the pest was found in the vegetation season characterised by the highest amount of rainfall with the lowest mean daily air temperature. The presence of potassium in a given fertiliser combination, the application of manure or combined application of manure and mineral fertilisation resulted in an enhanced resistance of maize plants to Fusarium ssp. fungi. The significantly greatest infestation of maize plants by the fungus Ustilago maydis Corda was recorded in the treatment in which only nitrogen was applied. In turn, the lowest percentage of plants with symptoms of this disease was recorded in the treatment with the application of potassium alone and in the application of potassium together with phosphorus. Keywords: Zea mays L., mineral fertilisation, manure, diseases, pests Journal: Plant Protection Science Pages: 174-182 Volume: 54 Issue: 3 Year: 2018 DOI: 10.17221/22/2017-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/22/2017-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201803-0006.txt Handle: RePEc:caa:jnlpps:v:54:y:2018:i:3:id:22-2017-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Jianmin Zeng Author-Workplace-Name: Yunnan Academy of Tobacco Agricultural Sciences, Kunming, P.R. China Author-Name: Zhe Zhang Author-Workplace-Name: Department of Plant Protection, Zhongkai University of Agriculture and Engineering, Guangzhou, P.R. China Author-Name: Meiyun Li Author-Workplace-Name: Yunnan Academy of Tobacco Agricultural Sciences, Kunming, P.R. China Author-Name: Xingfu Wu Author-Workplace-Name: Yunnan Academy of Tobacco Agricultural Sciences, Kunming, P.R. China Author-Name: Yongsan Zeng Author-Workplace-Name: Department of Plant Protection, Zhongkai University of Agriculture and Engineering, Guangzhou, P.R. China Author-Name: Yongping Li Author-Workplace-Name: Yunnan Academy of Tobacco Agricultural Sciences, Kunming, P.R. China Title: Distribution and molecular identification of Meloidogyne spp. parasitising flue-cured tobacco in Yunnan, China Abstract: Twenty-one populations of root-knot nematodes (RKNs) were recovered from diseased roots collected from flue-cured tobacco in 21 locations in Yunnan (China) during 2014-2015. Molecular diagnosis on species was performed based on characteristics of sequences of D2-D3 expansion domains of the 28S rDNA and sequence characterised amplified regions (SCAR). SCAR results identified 13 populations as Meloidogyne arenaria, six as M. javanica and two as M. incognita. Meloidogyne arenaria is the predominant species with a detection rate of 61.9%, followed by M. javanica (28.5%) and M. incognita (9.5%). The tobacco RKNs were mainly distributed in western, central, and southwestern Yunnan, particularly in the western areas. Meloidogyne arenaria and M. javanica mainly occurred in Lincang (southwestern Yunnan) and Yuxi (central Yunnan), and M. incognita only in Zhaotong (northeastern Yunnan). Phylogenetic analysis based on inferences using LSU D2-D3 sequence data revealed that all 21 populations of Meloidogyne in this study are in a monophyletic clade in relation to other RKNs. Keywords: tobacco, 28S LSU rDNA, SCAR, root-knot nematode Journal: Plant Protection Science Pages: 183-189 Volume: 54 Issue: 3 Year: 2018 DOI: 10.17221/82/2017-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/82/2017-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201803-0007.txt Handle: RePEc:caa:jnlpps:v:54:y:2018:i:3:id:82-2017-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Anna Wenda-Piesik Author-Workplace-Name: Department of Plant Growth Principles and Experimental Methodology and Department of Entomology and Molecular Phytopathology, Author-Name: Dariusz Piesik Author-Workplace-Name: UTP University of Science and Technology, Bydgoszcz, Poland Author-Name: Anita Krasińska Author-Workplace-Name: UTP University of Science and Technology, Bydgoszcz, Poland Author-Workplace-Name: Group of Economic and Administration Schools, Bydgoszcz, Poland$2 Title: Response of mated insects of both sexes of granary weevil to blends of volatiles - short communication Abstract: We report the behavioural responses of mated granary weevils to blends of cereal volatiles. Four doses were applied on filter paper (1, 10, 100, and 1000 ng/min in 50 µl of hexane applied on filter paper). A Y-tube experiment revealed that mated females of Sitophilus granarius were attracted to blend 1, 4, and 5 at concentrations of 1000, 100, 1, and 10 ng/min, respectively. Mated males were attracted only to blend 5 at a concentration of 10 ng/minute. Yet, the mated weevil females and males were repelled by the highest concentration (1000 ng/min) for all tested blends. Moreover, both mated sexes were repelled by 100 ng/min in blends 2, 3, 5, and 6. Additionally, females were repelled by 10 ng/min in blend 6. We claim that the blend of volatiles and their concentration can induce the insect behaviour. Keywords: ereal insects, cereal volatiles, attractants, repellents Journal: Plant Protection Science Pages: 190-193 Volume: 54 Issue: 3 Year: 2018 DOI: 10.17221/136/2017-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/136/2017-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201803-0008.txt Handle: RePEc:caa:jnlpps:v:54:y:2018:i:3:id:136-2017-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Chuan-Jie Zhang Author-Workplace-Name: Department of Plant Science, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Gwanak-gu, Seoul, South Korea Author-Name: Do-Soon Kim Title: Using leaf chlorophyll fluorescence for in-season diagnosing herbicide resistance in Echinochloa species at reproductive growth stage Abstract: The chlorophyll fluorescence measurement to diagnose herbicide resistant Echinochloa species at the reproductive (late) growth stage was applied. The significant correlation between Fv/Fm (chlorophyll fluorescence measurements) and fresh weight (whole plant test) and the statistical similarity of R/S ratios between the two tests demonstrated that the chlorophyll fluorescence test could be reliably used to diagnose herbicide resistant Echinochloa spp. at the reproductive growth stage in a shorter period of time (within 10 days) compared with the conventional whole plant test. Keywords: ACCase inhibitor, ALS inhibitor, chlorophyll fluorescence induction, resistance diagnosis, decision-making Journal: Plant Protection Science Pages: 194-202 Volume: 54 Issue: 3 Year: 2018 DOI: 10.17221/51/2017-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/51/2017-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201803-0009.txt Handle: RePEc:caa:jnlpps:v:54:y:2018:i:3:id:51-2017-PPS